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The map label for this gene is cynR [H]

Identifier: 77461386

GI number: 77461386

Start: 5806458

End: 5807354

Strand: Direct

Name: cynR [H]

Synonym: Pfl01_5165

Alternate gene names: 77461386

Gene position: 5806458-5807354 (Clockwise)

Preceding gene: 77461383

Following gene: 77461398

Centisome position: 90.18

GC content: 60.2

Gene sequence:

>897_bases
ATGGATTTCAAACAACTGCGTTATTTCGTCGCGGTCTATGAAGAAGGGCATGTCGGACGCGCCGCCGAGCGCCTGTCGAT
CTCTCAGCCCGCGCTGTCCCAGCAGATTCGCCAGCTGGAACAAAACCTCGACGTCACCCTGTTCGAACGCAGCAGCAAAC
GCCTGCTGCCGACCCTCGCCGCCCACACCTTGTACAACCACGCCTTGCCACTGCTCGACGGCATGCAGCGGGCGTGTGAG
GCGTTGGGTAACTTCAAAGGGCAGGCACTGCGCACATTGGCGATCGGCGTGCTGCAAACCGTGCACACCAGCCTGGTGCC
GCAAATGCTGGAGAGAGTGCGCAAGGCCCAGCCGCATCTGGTGGTGCAGATTTATGAACTGACGGGGCTTGAGATCGAGC
GGCGACTGCTCAACGGCTCGCTGGACATCGGCATCAGCTATCTACCGCCACGCCAGCCGGGGCTGCACGGCGTGATGTTG
TACGAAGATGAACTGACGCTGGTCATCCCGGCGGATCATCCGCTGCGGGAATTCAAGAAAGTCTCGATGAGTCAGGCAGC
CGAATTGCCGATGTTGCTATTGGGCGAAGAATTTCAGATCCGCCAGATCTGGCAGGCGCAACTGGCCAGCCTCGGACGAC
GCCCGCAAGTGCAGGCCGAATTGAACAATATGGTGGGGATTCTCGACAGCCTTCCGCATACCCGTCTGGCGACGGTGCTG
CCCGGTCGTTCGCAAAAAGAGTACGACGATCAGGATCTGCTGTGGAAACCGTTGAGTGAGCCGCGCGTGCCGTTGAAGGT
CGGGCTGGTGTGTCGCGATGTGCAACGCCAGCAGGCGCCATTGGCCTTGCTGCGAACGTTGCTGGAAGAGGTGATCAGCG
TGAGGACAAACCGCTGA

Upstream 100 bases:

>100_bases
GCGCTTTCCGGCCCTGTAAGCCTTGTGAATGCGGTTAGACTAGGCCGGTTCACGGCGTCAAGCCATTTCGATTTCCCAGC
ATTTTCGATAAGTTTTACTT

Downstream 100 bases:

>100_bases
AAGGGGCGCCGCCGCTGGACGCGTTGAGCTGAAAACTTTTCTGCGGGCATAAGAAAACCCCGCCGAAGCGGGGCTTTGCA
GACTGTTTCCCTGACATCCA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Cyn operon transcriptional activator [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MDFKQLRYFVAVYEEGHVGRAAERLSISQPALSQQIRQLEQNLDVTLFERSSKRLLPTLAAHTLYNHALPLLDGMQRACE
ALGNFKGQALRTLAIGVLQTVHTSLVPQMLERVRKAQPHLVVQIYELTGLEIERRLLNGSLDIGISYLPPRQPGLHGVML
YEDELTLVIPADHPLREFKKVSMSQAAELPMLLLGEEFQIRQIWQAQLASLGRRPQVQAELNNMVGILDSLPHTRLATVL
PGRSQKEYDDQDLLWKPLSEPRVPLKVGLVCRDVQRQQAPLALLRTLLEEVISVRTNR

Sequences:

>Translated_298_residues
MDFKQLRYFVAVYEEGHVGRAAERLSISQPALSQQIRQLEQNLDVTLFERSSKRLLPTLAAHTLYNHALPLLDGMQRACE
ALGNFKGQALRTLAIGVLQTVHTSLVPQMLERVRKAQPHLVVQIYELTGLEIERRLLNGSLDIGISYLPPRQPGLHGVML
YEDELTLVIPADHPLREFKKVSMSQAAELPMLLLGEEFQIRQIWQAQLASLGRRPQVQAELNNMVGILDSLPHTRLATVL
PGRSQKEYDDQDLLWKPLSEPRVPLKVGLVCRDVQRQQAPLALLRTLLEEVISVRTNR
>Mature_298_residues
MDFKQLRYFVAVYEEGHVGRAAERLSISQPALSQQIRQLEQNLDVTLFERSSKRLLPTLAAHTLYNHALPLLDGMQRACE
ALGNFKGQALRTLAIGVLQTVHTSLVPQMLERVRKAQPHLVVQIYELTGLEIERRLLNGSLDIGISYLPPRQPGLHGVML
YEDELTLVIPADHPLREFKKVSMSQAAELPMLLLGEEFQIRQIWQAQLASLGRRPQVQAELNNMVGILDSLPHTRLATVL
PGRSQKEYDDQDLLWKPLSEPRVPLKVGLVCRDVQRQQAPLALLRTLLEEVISVRTNR

Specific function: Positively regulates the cynTSX operon, and negatively regulates its own transcription. Binds specifically to the cynR- cynTSX intergenic region [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=249, Percent_Identity=32.5301204819277, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1788887, Length=261, Percent_Identity=26.8199233716475, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87081904, Length=212, Percent_Identity=31.6037735849057, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1788297, Length=203, Percent_Identity=33.0049261083744, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1787879, Length=293, Percent_Identity=29.3515358361775, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1790399, Length=277, Percent_Identity=27.0758122743682, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI2367136, Length=294, Percent_Identity=27.891156462585, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787601, Length=172, Percent_Identity=31.3953488372093, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788481, Length=265, Percent_Identity=27.9245283018868, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI87082132, Length=171, Percent_Identity=29.2397660818713, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI87081684, Length=174, Percent_Identity=26.4367816091954, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI145693105, Length=271, Percent_Identity=21.0332103321033, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788296, Length=201, Percent_Identity=26.865671641791, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1787530, Length=228, Percent_Identity=25.4385964912281, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1787589, Length=136, Percent_Identity=29.4117647058824, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33772; Mature: 33772

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFKQLRYFVAVYEEGHVGRAAERLSISQPALSQQIRQLEQNLDVTLFERSSKRLLPTLA
CCHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHH
AHTLYNHALPLLDGMQRACEALGNFKGQALRTLAIGVLQTVHTSLVPQMLERVRKAQPHL
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
VVQIYELTGLEIERRLLNGSLDIGISYLPPRQPGLHGVMLYEDELTLVIPADHPLREFKK
EEEEEECCCHHHHHHHHCCCCCCCEEECCCCCCCCCEEEEEECCEEEEECCCCHHHHHHH
VSMSQAAELPMLLLGEEFQIRQIWQAQLASLGRRPQVQAELNNMVGILDSLPHTRLATVL
HHHHHHHCCCHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHC
PGRSQKEYDDQDLLWKPLSEPRVPLKVGLVCRDVQRQQAPLALLRTLLEEVISVRTNR
CCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDFKQLRYFVAVYEEGHVGRAAERLSISQPALSQQIRQLEQNLDVTLFERSSKRLLPTLA
CCHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHH
AHTLYNHALPLLDGMQRACEALGNFKGQALRTLAIGVLQTVHTSLVPQMLERVRKAQPHL
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
VVQIYELTGLEIERRLLNGSLDIGISYLPPRQPGLHGVMLYEDELTLVIPADHPLREFKK
EEEEEECCCHHHHHHHHCCCCCCCEEECCCCCCCCCEEEEEECCEEEEECCCCHHHHHHH
VSMSQAAELPMLLLGEEFQIRQIWQAQLASLGRRPQVQAELNNMVGILDSLPHTRLATVL
HHHHHHHCCCHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHC
PGRSQKEYDDQDLLWKPLSEPRVPLKVGLVCRDVQRQQAPLALLRTLLEEVISVRTNR
CCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]