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The map label for this gene is ksgA

Identifier: 77461356

GI number: 77461356

Start: 5771184

End: 5772002

Strand: Direct

Name: ksgA

Synonym: Pfl01_5135

Alternate gene names: 77461356

Gene position: 5771184-5772002 (Clockwise)

Preceding gene: 77461355

Following gene: 77461357

Centisome position: 89.64

GC content: 62.88

Gene sequence:

>819_bases
ATGACCGAGCAATACCAACACAAGGCGCGCAAACGCTTTGGCCAGAACTTCCTGCACGATGCCGGCGTCATCGACCGCAT
CCTGCGCTCCATCAATGCCAAGGCCGGCGACCGCATGCTGGAAATCGGGCCGGGCCAGGGCGCACTGACCGCCGGCATCC
TCAACTCCGGCGCACAGCTGGATGTGGTGGAACTGGACAAGGACCTGATCCCGATCCTCAACCAGCAGTTCGCCGGCAAG
AGCAATTTCAACCTGCATCAGGGCGATGCGCTGAAGTTCGACTTCAACAGCCTGAACGCTGCGCCGAACAGCCTGCGTGT
GGTCGGCAACCTGCCGTACAACATCTCCACGCCGCTGATTTTCCATCTGCTGAACAACGCCGGCATCATCCGCGACATGC
ACTTCATGCTGCAGAAGGAAGTGGTCGAGCGTCTGGCCGCCGGTCCCGGTGGCGGTGACTGGGGCCGTCTGTCGATCATG
GTTCAGTACCACTGCCGGGTCGAGCACCTGTTCAACGTCGGCCCGGGCGCATTCAATCCGCCGCCGAAAGTCGACTCGGC
GATCGTCCGCCTGGTACCGCACGCGGTGCTGCCGCATCCGGCCAAGGATCATCGCCTGCTCGAGCGCGTGGTGCGTGAAG
CCTTCAATCAGCGCCGCAAGACCCTGCGCAACACCCTCAAGCAGTTGCTGAGCAATGCCGAAATCGAAGCCGCCGGAGTC
GATGGCAGCCTGCGCCCCGAGCAACTCGATCTGGCCGCGTTCGTACGTCTGGCCGACAAGCTCGCCGAACAGCCGGCGAA
AGCACCTGAAGCCGACTGA

Upstream 100 bases:

>100_bases
ACCATGGCACCGCCCTGGATCTGGCCGGCAGCGGCAGGATCGACACCGGCAGCCTGGAAACCGCCCTGGAAACCGCCTAC
CAGATGGCCGAGACCCGTTT

Downstream 100 bases:

>100_bases
CCGGCAATGATCGCTATCGGGCAGGATGCCAACTCTGGTCTACTGCCCGATACTTGCCTAGACTGAATCCCAATCAGCTA
CGCCCCGCGTCCCGCTTCGT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MTEQYQHKARKRFGQNFLHDAGVIDRILRSINAKAGDRMLEIGPGQGALTAGILNSGAQLDVVELDKDLIPILNQQFAGK
SNFNLHQGDALKFDFNSLNAAPNSLRVVGNLPYNISTPLIFHLLNNAGIIRDMHFMLQKEVVERLAAGPGGGDWGRLSIM
VQYHCRVEHLFNVGPGAFNPPPKVDSAIVRLVPHAVLPHPAKDHRLLERVVREAFNQRRKTLRNTLKQLLSNAEIEAAGV
DGSLRPEQLDLAAFVRLADKLAEQPAKAPEAD

Sequences:

>Translated_272_residues
MTEQYQHKARKRFGQNFLHDAGVIDRILRSINAKAGDRMLEIGPGQGALTAGILNSGAQLDVVELDKDLIPILNQQFAGK
SNFNLHQGDALKFDFNSLNAAPNSLRVVGNLPYNISTPLIFHLLNNAGIIRDMHFMLQKEVVERLAAGPGGGDWGRLSIM
VQYHCRVEHLFNVGPGAFNPPPKVDSAIVRLVPHAVLPHPAKDHRLLERVVREAFNQRRKTLRNTLKQLLSNAEIEAAGV
DGSLRPEQLDLAAFVRLADKLAEQPAKAPEAD
>Mature_271_residues
TEQYQHKARKRFGQNFLHDAGVIDRILRSINAKAGDRMLEIGPGQGALTAGILNSGAQLDVVELDKDLIPILNQQFAGKS
NFNLHQGDALKFDFNSLNAAPNSLRVVGNLPYNISTPLIFHLLNNAGIIRDMHFMLQKEVVERLAAGPGGGDWGRLSIMV
QYHCRVEHLFNVGPGAFNPPPKVDSAIVRLVPHAVLPHPAKDHRLLERVVREAFNQRRKTLRNTLKQLLSNAEIEAAGVD
GSLRPEQLDLAAFVRLADKLAEQPAKAPEAD

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=280, Percent_Identity=35.3571428571429, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI7657198, Length=247, Percent_Identity=33.6032388663968, Blast_Score=125, Evalue=6e-29,
Organism=Escherichia coli, GI1786236, Length=258, Percent_Identity=52.7131782945737, Blast_Score=271, Evalue=3e-74,
Organism=Caenorhabditis elegans, GI25141369, Length=297, Percent_Identity=30.976430976431, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI25146882, Length=212, Percent_Identity=35.377358490566, Blast_Score=126, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6324989, Length=218, Percent_Identity=31.1926605504587, Blast_Score=104, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21358017, Length=220, Percent_Identity=35.9090909090909, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI21357273, Length=294, Percent_Identity=32.312925170068, Blast_Score=122, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_PSEPF (Q3K5T2)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_350863.1
- ProteinModelPortal:   Q3K5T2
- SMR:   Q3K5T2
- STRING:   Q3K5T2
- GeneID:   3717266
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_5135
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   RAENLTP
- ProtClustDB:   PRK00274
- BioCyc:   PFLU205922:PFL_5135-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 29998; Mature: 29867

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: PS01131 RRNA_A_DIMETH; PS00290 IG_MHC

Important sites: BINDING 16-16 BINDING 18-18 BINDING 43-43 BINDING 64-64 BINDING 89-89 BINDING 110-110

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQYQHKARKRFGQNFLHDAGVIDRILRSINAKAGDRMLEIGPGQGALTAGILNSGAQL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHCCCCEE
DVVELDKDLIPILNQQFAGKSNFNLHQGDALKFDFNSLNAAPNSLRVVGNLPYNISTPLI
EHHHHCHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHCCCCCCCEEEEECCCCCCCHHHH
FHLLNNAGIIRDMHFMLQKEVVERLAAGPGGGDWGRLSIMVQYHCRVEHLFNVGPGAFNP
HHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEHHHHHHHHCCCCCCCCC
PPKVDSAIVRLVPHAVLPHPAKDHRLLERVVREAFNQRRKTLRNTLKQLLSNAEIEAAGV
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
DGSLRPEQLDLAAFVRLADKLAEQPAKAPEAD
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TEQYQHKARKRFGQNFLHDAGVIDRILRSINAKAGDRMLEIGPGQGALTAGILNSGAQL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHCCCCEE
DVVELDKDLIPILNQQFAGKSNFNLHQGDALKFDFNSLNAAPNSLRVVGNLPYNISTPLI
EHHHHCHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHCCCCCCCEEEEECCCCCCCHHHH
FHLLNNAGIIRDMHFMLQKEVVERLAAGPGGGDWGRLSIMVQYHCRVEHLFNVGPGAFNP
HHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEHHHHHHHHCCCCCCCCC
PPKVDSAIVRLVPHAVLPHPAKDHRLLERVVREAFNQRRKTLRNTLKQLLSNAEIEAAGV
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
DGSLRPEQLDLAAFVRLADKLAEQPAKAPEAD
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA