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The map label for this gene is potD-A [H]

Identifier: 77461345

GI number: 77461345

Start: 5756913

End: 5757956

Strand: Reverse

Name: potD-A [H]

Synonym: Pfl01_5124

Alternate gene names: 77461345

Gene position: 5757956-5756913 (Counterclockwise)

Preceding gene: 77461346

Following gene: 77461344

Centisome position: 89.43

GC content: 60.34

Gene sequence:

>1044_bases
ATGTTGAGATCCCTGAAGTTCACAGCCCTGACCCTGGGCCTGATGGGTGCGGCAAGCGCAATGGCCGCTGGGCCGGATCT
GACCGTGGTGTCGTTTGGCGGGGCGAACAAGGCGGCGCAAGTCAAAGCCTTCTACGCACCGTGGGAAGCAGCGGGCAACG
GCAAGATCGTCGCCGGCGAGTACAACGGTGAGATGGCCAAGGTCAAAGCCATGGTCGACACCAAGAGCGTGTCCTGGGAT
CTGGTCGAGGTTGAATCGCCGGAGCTGTCCCGTGGCTGCGACGAAGACATGTTCGAACAACTGGATCCTGCGCTGTTCGG
CAAATCCGAAGATTACGTCAAAGGCGCGATCCAGCCGTGCGGCGTGGGCTTCTTCGTGTGGTCGACCGTGCTGGCCTACA
ACGCCGACAAGCTGAAAACCGCACCGACCAGCTGGGCGGATTTCTGGGACACCAAGAAATTCCCGGGCAAGCGCGGCCTG
CGCAAAGGCGCCAAGTACACCCTCGAATTCGCACTGATGGCCGACGGCGTTGCGCCGAAAGACGTGTACAAGGAACTGGC
CAGCAAGGGCGGTCAGGATCGCGCATTCAAGAAACTGGATGAGCTGAAACCGAACATCCAGTGGTGGGAAGCCGGCGCTC
AGCCGCCGCAATACCTCGCTTCCGGTGACGTGGTCATGAGCTCGGCCTACAACGGTCGTATCGCTGCGGTGCAGAAAGAG
TCCAACCTGAAAGTGGTGTGGAACGGCGGCATCTACGACTTCGACGCCTGGGCCATCCCGAAAGGCCTGGACGCCAAGCG
CGCCGAAGCGGCGAAGAAATTCATCGCGTTCTCGGTGCAGCCACAACAACAGAAGACCTACTCGGAGAACATCGCCTACG
GCCCGGCCAACTCTCAGGCAGTGCCATTGCTGGACAAGGCGATCCTGAAAGACATGCCGACCACCCCGGAAAACATCGCC
AACCAGGTGCAGATCGATGTCAGCTTCTGGGCTGACAACGGCGAGCAACTGGAGCAGCGCTTCAATTCCTGGGCTGCGAA
GTAA

Upstream 100 bases:

>100_bases
CTGGCAGGTCGAGCACGTTCGCGCGCTCGATCCTCTTCTAGAGGCGCACTGAAACGCCCCGCAATACCAACACCAACCCT
GCACGTGGAGAGAACAATAA

Downstream 100 bases:

>100_bases
GGCTGATGCATACGCGGCGTCACCCCCAGGGTGACGCCGTCATTTAAAAGATTTGCGGAGTACGTCATGGCCATCGCCGT
TCCCCTGAACGAGGGCAACA

Product: extracellular solute-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MLRSLKFTALTLGLMGAASAMAAGPDLTVVSFGGANKAAQVKAFYAPWEAAGNGKIVAGEYNGEMAKVKAMVDTKSVSWD
LVEVESPELSRGCDEDMFEQLDPALFGKSEDYVKGAIQPCGVGFFVWSTVLAYNADKLKTAPTSWADFWDTKKFPGKRGL
RKGAKYTLEFALMADGVAPKDVYKELASKGGQDRAFKKLDELKPNIQWWEAGAQPPQYLASGDVVMSSAYNGRIAAVQKE
SNLKVVWNGGIYDFDAWAIPKGLDAKRAEAAKKFIAFSVQPQQQKTYSENIAYGPANSQAVPLLDKAILKDMPTTPENIA
NQVQIDVSFWADNGEQLEQRFNSWAAK

Sequences:

>Translated_347_residues
MLRSLKFTALTLGLMGAASAMAAGPDLTVVSFGGANKAAQVKAFYAPWEAAGNGKIVAGEYNGEMAKVKAMVDTKSVSWD
LVEVESPELSRGCDEDMFEQLDPALFGKSEDYVKGAIQPCGVGFFVWSTVLAYNADKLKTAPTSWADFWDTKKFPGKRGL
RKGAKYTLEFALMADGVAPKDVYKELASKGGQDRAFKKLDELKPNIQWWEAGAQPPQYLASGDVVMSSAYNGRIAAVQKE
SNLKVVWNGGIYDFDAWAIPKGLDAKRAEAAKKFIAFSVQPQQQKTYSENIAYGPANSQAVPLLDKAILKDMPTTPENIA
NQVQIDVSFWADNGEQLEQRFNSWAAK
>Mature_347_residues
MLRSLKFTALTLGLMGAASAMAAGPDLTVVSFGGANKAAQVKAFYAPWEAAGNGKIVAGEYNGEMAKVKAMVDTKSVSWD
LVEVESPELSRGCDEDMFEQLDPALFGKSEDYVKGAIQPCGVGFFVWSTVLAYNADKLKTAPTSWADFWDTKKFPGKRGL
RKGAKYTLEFALMADGVAPKDVYKELASKGGQDRAFKKLDELKPNIQWWEAGAQPPQYLASGDVVMSSAYNGRIAAVQKE
SNLKVVWNGGIYDFDAWAIPKGLDAKRAEAAKKFIAFSVQPQQQKTYSENIAYGPANSQAVPLLDKAILKDMPTTPENIA
NQVQIDVSFWADNGEQLEQRFNSWAAK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=230, Percent_Identity=28.2608695652174, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37854; Mature: 37854

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRSLKFTALTLGLMGAASAMAAGPDLTVVSFGGANKAAQVKAFYAPWEAAGNGKIVAGE
CCCCEEHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEEECCCCCCCCCEEEEEE
YNGEMAKVKAMVDTKSVSWDLVEVESPELSRGCDEDMFEQLDPALFGKSEDYVKGAIQPC
CCCCEEEEEEEEECCCCCEEEEEECCCHHHCCCCHHHHHHCCHHHCCCCHHHHHCCCCCC
GVGFFVWSTVLAYNADKLKTAPTSWADFWDTKKFPGKRGLRKGAKYTLEFALMADGVAPK
CCHHHHHHHHHHCCCHHHCCCCCCHHHHHCCCCCCCHHHHHCCCCEEEEEEEEECCCCHH
DVYKELASKGGQDRAFKKLDELKPNIQWWEAGAQPPQYLASGDVVMSSAYNGRIAAVQKE
HHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCCCHHHHCCCCEEEECCCCCEEEEEEEC
SNLKVVWNGGIYDFDAWAIPKGLDAKRAEAAKKFIAFSVQPQQQKTYSENIAYGPANSQA
CCEEEEECCCEEECCCCCCCCCCCHHHHHHHHHHHEEECCCHHHHHHHCCCEECCCCCCC
VPLLDKAILKDMPTTPENIANQVQIDVSFWADNGEQLEQRFNSWAAK
CHHHHHHHHHCCCCCHHHHCCEEEEEEEEECCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MLRSLKFTALTLGLMGAASAMAAGPDLTVVSFGGANKAAQVKAFYAPWEAAGNGKIVAGE
CCCCEEHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEEECCCCCCCCCEEEEEE
YNGEMAKVKAMVDTKSVSWDLVEVESPELSRGCDEDMFEQLDPALFGKSEDYVKGAIQPC
CCCCEEEEEEEEECCCCCEEEEEECCCHHHCCCCHHHHHHCCHHHCCCCHHHHHCCCCCC
GVGFFVWSTVLAYNADKLKTAPTSWADFWDTKKFPGKRGLRKGAKYTLEFALMADGVAPK
CCHHHHHHHHHHCCCHHHCCCCCCHHHHHCCCCCCCHHHHHCCCCEEEEEEEEECCCCHH
DVYKELASKGGQDRAFKKLDELKPNIQWWEAGAQPPQYLASGDVVMSSAYNGRIAAVQKE
HHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCCCHHHHCCCCEEEECCCCCEEEEEEEC
SNLKVVWNGGIYDFDAWAIPKGLDAKRAEAAKKFIAFSVQPQQQKTYSENIAYGPANSQA
CCEEEEECCCEEECCCCCCCCCCCHHHHHHHHHHHEEECCCHHHHHHHCCCEECCCCCCC
VPLLDKAILKDMPTTPENIANQVQIDVSFWADNGEQLEQRFNSWAAK
CHHHHHHHHHCCCCCHHHHCCEEEEEEEEECCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]