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The map label for this gene is trpE [H]

Identifier: 77461339

GI number: 77461339

Start: 5750351

End: 5751829

Strand: Reverse

Name: trpE [H]

Synonym: Pfl01_5118

Alternate gene names: 77461339

Gene position: 5751829-5750351 (Counterclockwise)

Preceding gene: 77461340

Following gene: 77461337

Centisome position: 89.34

GC content: 62.68

Gene sequence:

>1479_bases
ATGATCCGCGAAGAATTCCTGCGTCTGGCCGCTGACGGCTACAACCGCATCCCGCTGGCCTGCGAAACCCTGGCCGACTT
CGACACCCCGCTGTCGATCTACCTGAAACTGGCCGACCAGCCCAACTCCTACCTGCTCGAATCGGTGCAGGGCGGCGAGA
AATGGGGCCGTTACTCGATCATCGGCCTGCCGTGCCGCACCGTGCTGCGGGTTCACGATCATCACGTGAGCATCACCGTC
GATGGCGTCGAGACCGAAAGCCACGACGTCGAAGACCCGCTGGCCTTCGTCGAAACTTTCAAGGCCCGCTACAACGTGCC
GACCATCGCCGGTCTGCCGCGCTTCAACGGCGGTCTGGTGGGTTACTTCGGTTACGACTGTGTGCGTTATGTAGAGAAGC
GCCTCGGCAAATGCCCGAATCCGGATCCGCTGGGCGTGCCGGACATTCTGCTGATGGTCTCCGATGCGGTGGTGGTGTTC
GACAACCTCGCCGGCAAGATGCACGCGATTGTGCTGGCCGATCCGGCGCAGGCCGATGCCTACGAACAGGGCCAGGCGCA
ATTGCAGCAACTGCTGGAAAAACTGCGTCAGCCGATCACCCCGCGCCGTGGCCTGGATTTCAGCAAGCAGCAGGCGGCCG
AGCCGGTGTTCCGCTCCAGTTTCACCCAGGACGACTACGAAAAAGCCGTCGACACCATCAAGGAATACATCCTCGCCGGT
GACTGCATGCAGGTCGTGCCGTCCCAGCGCATGTCGATCGACTTCAAGGCGGCGCCGATCGATCTGTACCGCGCGCTGCG
TTGCTTCAACCCGACGCCGTACATGTACTTCTTCAACTTCGGCGACTTCCACGTCGTCGGCAGTTCGCCGGAAGTGCTGG
TGCGGGTCGAAGACAACCTGATCACCGTGCGCCCGATTGCCGGCACCCGTCCTCGCGGGGCTACCGAAGAAGCGGACGTG
GCGCTGGAAGAAGACCTGCTGTCCGACGACAAGGAGATCGCCGAGCACCTGATGCTGATCGACCTCGGTCGCAACGACAC
CGGGCGCGTATCGGAAATCGGCTCGGTGAAACTTACCGAGAAGATGGTCATCGAGCGTTATTCCAACGTGATGCACATCG
TGTCCAACGTCACCGGCCAGTTGAAGGCCGGGCTGACGGCGATGGACGCACTGCGGGCGATCCTGCCGGCCGGCACCTTG
TCCGGCGCACCGAAGATCCGCGCGATGGAAATCATCGACGAGCTGGAGCCGGTCAAGCGTGGTGTGTACGGCGGCGCGGT
CGGATACTTCGCCTGGAACGGCAACATGGACACCGCGATTGCGATCCGTACCGCAGTGATCAAGAACGGCGAGTTGCATG
TGCAGGCCGGTGGCGGCATCGTCGCCGATTCGGTGCCGGCGCTGGAGTGGGAAGAAACCATCAACAAGCGCCGGGCGATG
TTCCGCGCTGTTGCGCTGGCCGAGCAAACCCCGGACTGA

Upstream 100 bases:

>100_bases
CCCTGGCCCGCTGGCGTTGGCGCGCCTGACTTGTTCCTGGCCGGCTTGCCGGCGCGTTTGCACACCTGACTGATTTGCAC
CTCACACCACGAGGCACCCT

Downstream 100 bases:

>100_bases
GTCATTGATGATTCAGCCTAAAAAAATGCGGCGCCTGTGAAGGCGCCGCATTTTTTTTGCCTGCGAATCAGAAGTCGAGC
GCTACGCCGACGTTGATCCC

Product: anthranilate synthase component I

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MIREEFLRLAADGYNRIPLACETLADFDTPLSIYLKLADQPNSYLLESVQGGEKWGRYSIIGLPCRTVLRVHDHHVSITV
DGVETESHDVEDPLAFVETFKARYNVPTIAGLPRFNGGLVGYFGYDCVRYVEKRLGKCPNPDPLGVPDILLMVSDAVVVF
DNLAGKMHAIVLADPAQADAYEQGQAQLQQLLEKLRQPITPRRGLDFSKQQAAEPVFRSSFTQDDYEKAVDTIKEYILAG
DCMQVVPSQRMSIDFKAAPIDLYRALRCFNPTPYMYFFNFGDFHVVGSSPEVLVRVEDNLITVRPIAGTRPRGATEEADV
ALEEDLLSDDKEIAEHLMLIDLGRNDTGRVSEIGSVKLTEKMVIERYSNVMHIVSNVTGQLKAGLTAMDALRAILPAGTL
SGAPKIRAMEIIDELEPVKRGVYGGAVGYFAWNGNMDTAIAIRTAVIKNGELHVQAGGGIVADSVPALEWEETINKRRAM
FRAVALAEQTPD

Sequences:

>Translated_492_residues
MIREEFLRLAADGYNRIPLACETLADFDTPLSIYLKLADQPNSYLLESVQGGEKWGRYSIIGLPCRTVLRVHDHHVSITV
DGVETESHDVEDPLAFVETFKARYNVPTIAGLPRFNGGLVGYFGYDCVRYVEKRLGKCPNPDPLGVPDILLMVSDAVVVF
DNLAGKMHAIVLADPAQADAYEQGQAQLQQLLEKLRQPITPRRGLDFSKQQAAEPVFRSSFTQDDYEKAVDTIKEYILAG
DCMQVVPSQRMSIDFKAAPIDLYRALRCFNPTPYMYFFNFGDFHVVGSSPEVLVRVEDNLITVRPIAGTRPRGATEEADV
ALEEDLLSDDKEIAEHLMLIDLGRNDTGRVSEIGSVKLTEKMVIERYSNVMHIVSNVTGQLKAGLTAMDALRAILPAGTL
SGAPKIRAMEIIDELEPVKRGVYGGAVGYFAWNGNMDTAIAIRTAVIKNGELHVQAGGGIVADSVPALEWEETINKRRAM
FRAVALAEQTPD
>Mature_492_residues
MIREEFLRLAADGYNRIPLACETLADFDTPLSIYLKLADQPNSYLLESVQGGEKWGRYSIIGLPCRTVLRVHDHHVSITV
DGVETESHDVEDPLAFVETFKARYNVPTIAGLPRFNGGLVGYFGYDCVRYVEKRLGKCPNPDPLGVPDILLMVSDAVVVF
DNLAGKMHAIVLADPAQADAYEQGQAQLQQLLEKLRQPITPRRGLDFSKQQAAEPVFRSSFTQDDYEKAVDTIKEYILAG
DCMQVVPSQRMSIDFKAAPIDLYRALRCFNPTPYMYFFNFGDFHVVGSSPEVLVRVEDNLITVRPIAGTRPRGATEEADV
ALEEDLLSDDKEIAEHLMLIDLGRNDTGRVSEIGSVKLTEKMVIERYSNVMHIVSNVTGQLKAGLTAMDALRAILPAGTL
SGAPKIRAMEIIDELEPVKRGVYGGAVGYFAWNGNMDTAIAIRTAVIKNGELHVQAGGGIVADSVPALEWEETINKRRAM
FRAVALAEQTPD

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=408, Percent_Identity=39.7058823529412, Blast_Score=245, Evalue=6e-66,
Organism=Escherichia coli, GI1788114, Length=445, Percent_Identity=33.2584269662921, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1786809, Length=285, Percent_Identity=27.719298245614, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87082077, Length=283, Percent_Identity=26.1484098939929, Blast_Score=80, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6320935, Length=477, Percent_Identity=39.4129979035639, Blast_Score=324, Evalue=2e-89,
Organism=Saccharomyces cerevisiae, GI6324361, Length=393, Percent_Identity=28.498727735369, Blast_Score=133, Evalue=8e-32,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005256
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 54349; Mature: 54349

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIREEFLRLAADGYNRIPLACETLADFDTPLSIYLKLADQPNSYLLESVQGGEKWGRYSI
CCHHHHHHHHHCCCCCCCCHHHHHHCCCCCEEEEEEECCCCCHHHHHHHCCCCCCCCEEE
IGLPCRTVLRVHDHHVSITVDGVETESHDVEDPLAFVETFKARYNVPTIAGLPRFNGGLV
EECCHHHHHHEECCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEE
GYFGYDCVRYVEKRLGKCPNPDPLGVPDILLMVSDAVVVFDNLAGKMHAIVLADPAQADA
EHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCH
YEQGQAQLQQLLEKLRQPITPRRGLDFSKQQAAEPVFRSSFTQDDYEKAVDTIKEYILAG
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHCC
DCMQVVPSQRMSIDFKAAPIDLYRALRCFNPTPYMYFFNFGDFHVVGSSPEVLVRVEDNL
CHHHHCCCCCCEEEEECCCHHHHHHHHHCCCCCEEEEEECCCEEEECCCCCEEEEEECCE
ITVRPIAGTRPRGATEEADVALEEDLLSDDKEIAEHLMLIDLGRNDTGRVSEIGSVKLTE
EEEEECCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHEEEEECCCCCCCCHHCCCCCHHHH
KMVIERYSNVMHIVSNVTGQLKAGLTAMDALRAILPAGTLSGAPKIRAMEIIDELEPVKR
HHHHHHHHHHHHHHHHCCHHHHHCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
GVYGGAVGYFAWNGNMDTAIAIRTAVIKNGELHVQAGGGIVADSVPALEWEETINKRRAM
CCCCCEEEEEEECCCCCCHHEEEEEEEECCEEEEEECCCEEECCCCCCCHHHHHHHHHHH
FRAVALAEQTPD
HHHHHHHHCCCC
>Mature Secondary Structure
MIREEFLRLAADGYNRIPLACETLADFDTPLSIYLKLADQPNSYLLESVQGGEKWGRYSI
CCHHHHHHHHHCCCCCCCCHHHHHHCCCCCEEEEEEECCCCCHHHHHHHCCCCCCCCEEE
IGLPCRTVLRVHDHHVSITVDGVETESHDVEDPLAFVETFKARYNVPTIAGLPRFNGGLV
EECCHHHHHHEECCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEE
GYFGYDCVRYVEKRLGKCPNPDPLGVPDILLMVSDAVVVFDNLAGKMHAIVLADPAQADA
EHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCH
YEQGQAQLQQLLEKLRQPITPRRGLDFSKQQAAEPVFRSSFTQDDYEKAVDTIKEYILAG
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHCC
DCMQVVPSQRMSIDFKAAPIDLYRALRCFNPTPYMYFFNFGDFHVVGSSPEVLVRVEDNL
CHHHHCCCCCCEEEEECCCHHHHHHHHHCCCCCEEEEEECCCEEEECCCCCEEEEEECCE
ITVRPIAGTRPRGATEEADVALEEDLLSDDKEIAEHLMLIDLGRNDTGRVSEIGSVKLTE
EEEEECCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHEEEEECCCCCCCCHHCCCCCHHHH
KMVIERYSNVMHIVSNVTGQLKAGLTAMDALRAILPAGTLSGAPKIRAMEIIDELEPVKR
HHHHHHHHHHHHHHHHCCHHHHHCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
GVYGGAVGYFAWNGNMDTAIAIRTAVIKNGELHVQAGGGIVADSVPALEWEETINKRRAM
CCCCCEEEEEEECCCCCCHHEEEEEEEECCEEEEEECCCEEECCCCCCCHHHHHHHHHHH
FRAVALAEQTPD
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2404959 [H]