Click here to switch to the map view.

The map label for this gene is birA [H]

Identifier: 77461317

GI number: 77461317

Start: 5725257

End: 5726210

Strand: Reverse

Name: birA [H]

Synonym: Pfl01_5096

Alternate gene names: 77461317

Gene position: 5726210-5725257 (Counterclockwise)

Preceding gene: 77461319

Following gene: 77461316

Centisome position: 88.94

GC content: 54.51

Gene sequence:

>954_bases
ATGCTGACGTTGTTGAACCTTCTGAAGGATGGTCGATTCCATTCCGGTCAGGCTCTGGGGGCTGCCCTGGGAATTAGTCG
AAGTGCGGTGTGGAAGCAGCTTCAGCACCTGGAGGCTGAGCTTGGGTTATCCATACATAAAGTCCGGGGGCGGGGCTATC
AATTAGCCTCGCCATTGACTTTGCTTGACTCGCTCGAGATAGCGGAAAGTCTTCCTGGCTGGTCTGTCAGTATCTTTGAT
TCCGTCGACTCTACTAATGCTGAATCGTTGCGCGCGGTGGATCGGGGGCGGGCGGCTCCCTTTCTTGTACTGGCCGAGCT
CCAAAGATCAGGGCGTGGGCGCAGGGGGCGTAAATGGGTGAGTCCGTTTGCTGAAAACCTCTATTACAGCCTCGTGTTGC
GCATCGATGGTGGGATGCGTCAGCTTGAGGGGTTAAGTCTTGTTGTTGGTCTCGCGGTCATGCAGGCCTTGCATGCGCTT
GGGGTGCCAGGTGCAGGGCTGAAATGGCCCAATGATGTATTGGTCGGGCGAAAAAAAATCGCCGGGATCCTGTTGGAGCT
GGTTGGCGATCCGGCAGACGTGTGTCATGTAGTGTTGGGCATAGGCATTAATGTGAACATGCAAGCTACAGATGAAGTCG
ATCAGCAATGGACTTCAATGCGTCTGGAGTCGGGTAAATCTTGTGATCGCAATATTCTGGCGGTTGAGCTGAGTAAGCAG
CTGCAACTCTATTTGCAGCGTCACCAATCGGGCGGCTTCTCTGTGCTTCGGGCGGAGTGGGAGGCGAGCCATTTGTGGCA
GGGGCAGCCGGTTTCTCTCACTGCGGGGGTCAATCAAGTAGATGGTGTGGTGCTGGGAATCGATAATCAAGGCGCACTGC
GGCTAAAGGTAGACGGTGTGGAAAAAGTCTTTAGTGGTGGTGAGCTCAGCTTGAGGTTGCGTGATGATTCTTGA

Upstream 100 bases:

>100_bases
TGGGGTTTTGTTTTGCCCGCAGGAAATTGGATTGTTTGAATTCTATGCAGCGGCCCGGTGCATGGCTATTATTCGGGCCT
CATTCTGAGGCGAGACCTGC

Downstream 100 bases:

>100_bases
GCTTGATTGTGGAAACAGCTTTATCAAGTGGCGTGTGCTGCGGGCTGAGGCGGGAGCCTTGATCGCGGAAGGAGTGGTCG
ACTCTGATGGCGCCTTGGTC

Product: biotin--protein ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MLTLLNLLKDGRFHSGQALGAALGISRSAVWKQLQHLEAELGLSIHKVRGRGYQLASPLTLLDSLEIAESLPGWSVSIFD
SVDSTNAESLRAVDRGRAAPFLVLAELQRSGRGRRGRKWVSPFAENLYYSLVLRIDGGMRQLEGLSLVVGLAVMQALHAL
GVPGAGLKWPNDVLVGRKKIAGILLELVGDPADVCHVVLGIGINVNMQATDEVDQQWTSMRLESGKSCDRNILAVELSKQ
LQLYLQRHQSGGFSVLRAEWEASHLWQGQPVSLTAGVNQVDGVVLGIDNQGALRLKVDGVEKVFSGGELSLRLRDDS

Sequences:

>Translated_317_residues
MLTLLNLLKDGRFHSGQALGAALGISRSAVWKQLQHLEAELGLSIHKVRGRGYQLASPLTLLDSLEIAESLPGWSVSIFD
SVDSTNAESLRAVDRGRAAPFLVLAELQRSGRGRRGRKWVSPFAENLYYSLVLRIDGGMRQLEGLSLVVGLAVMQALHAL
GVPGAGLKWPNDVLVGRKKIAGILLELVGDPADVCHVVLGIGINVNMQATDEVDQQWTSMRLESGKSCDRNILAVELSKQ
LQLYLQRHQSGGFSVLRAEWEASHLWQGQPVSLTAGVNQVDGVVLGIDNQGALRLKVDGVEKVFSGGELSLRLRDDS
>Mature_317_residues
MLTLLNLLKDGRFHSGQALGAALGISRSAVWKQLQHLEAELGLSIHKVRGRGYQLASPLTLLDSLEIAESLPGWSVSIFD
SVDSTNAESLRAVDRGRAAPFLVLAELQRSGRGRRGRKWVSPFAENLYYSLVLRIDGGMRQLEGLSLVVGLAVMQALHAL
GVPGAGLKWPNDVLVGRKKIAGILLELVGDPADVCHVVLGIGINVNMQATDEVDQQWTSMRLESGKSCDRNILAVELSKQ
LQLYLQRHQSGGFSVLRAEWEASHLWQGQPVSLTAGVNQVDGVVLGIDNQGALRLKVDGVEKVFSGGELSLRLRDDS

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=313, Percent_Identity=40.5750798722045, Blast_Score=224, Evalue=8e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR004409
- InterPro:   IPR003142
- InterPro:   IPR004143
- InterPro:   IPR013196
- InterPro:   IPR008988
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB; PF08279 HTH_11 [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 34380; Mature: 34380

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTLLNLLKDGRFHSGQALGAALGISRSAVWKQLQHLEAELGLSIHKVRGRGYQLASPLT
CHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCEEEEECCCCEECCCCHH
LLDSLEIAESLPGWSVSIFDSVDSTNAESLRAVDRGRAAPFLVLAELQRSGRGRRGRKWV
HHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHH
SPFAENLYYSLVLRIDGGMRQLEGLSLVVGLAVMQALHALGVPGAGLKWPNDVLVGRKKI
HHHHHHHEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
AGILLELVGDPADVCHVVLGIGINVNMQATDEVDQQWTSMRLESGKSCDRNILAVELSKQ
HHHHHHHHCCHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCEEEEHHHHH
LQLYLQRHQSGGFSVLRAEWEASHLWQGQPVSLTAGVNQVDGVVLGIDNQGALRLKVDGV
HHHHHHHHCCCCCEEEEECCCHHHCCCCCCEEEECCCCCCCEEEEEECCCCEEEEEECCH
EKVFSGGELSLRLRDDS
HHHHCCCEEEEEEECCC
>Mature Secondary Structure
MLTLLNLLKDGRFHSGQALGAALGISRSAVWKQLQHLEAELGLSIHKVRGRGYQLASPLT
CHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCEEEEECCCCEECCCCHH
LLDSLEIAESLPGWSVSIFDSVDSTNAESLRAVDRGRAAPFLVLAELQRSGRGRRGRKWV
HHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHH
SPFAENLYYSLVLRIDGGMRQLEGLSLVVGLAVMQALHALGVPGAGLKWPNDVLVGRKKI
HHHHHHHEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
AGILLELVGDPADVCHVVLGIGINVNMQATDEVDQQWTSMRLESGKSCDRNILAVELSKQ
HHHHHHHHCCHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCEEEEHHHHH
LQLYLQRHQSGGFSVLRAEWEASHLWQGQPVSLTAGVNQVDGVVLGIDNQGALRLKVDGV
HHHHHHHHCCCCCEEEEECCCHHHCCCCCCEEEECCCCCCCEEEEEECCCCEEEEEECCH
EKVFSGGELSLRLRDDS
HHHHCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3899863; 3536662; 8265357; 9278503; 1311302; 1328157; 1409631; 11353844 [H]