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The map label for this gene is yddQ [H]

Identifier: 77461263

GI number: 77461263

Start: 5679358

End: 5679912

Strand: Direct

Name: yddQ [H]

Synonym: Pfl01_5042

Alternate gene names: 77461263

Gene position: 5679358-5679912 (Clockwise)

Preceding gene: 77461259

Following gene: 77461264

Centisome position: 88.21

GC content: 64.32

Gene sequence:

>555_bases
ATGGCCAAGCAAGCGCTCATCGTAGTCGATATCCAGAACGACTACTTCCCCCAAGGCAAGTGGCCGCTGGCCGGTGCCGA
CGCCGCCGCCGACAACGCCGCCCGGTTGATCGCGGCCTTCCGCGAGGCCGGCGATTCGGTGGTGCACATCCGCCACGAAT
TCACCTCGCCCGAGGCGCCGTTTTTCACTCCGGGCTCCGAGGGCGCCAAGCTGCATCCGAAAGTGCTCAACCGCGCCGAC
GAGCCGGTGGTCCTCAAGCACTTCGTCAACTCGTTCCGCGAAACCGAGCTGCAATCGATCCTCGACGAGCAAGGCATCAA
GGAGCTGGTGGTGGTCGGCAGCATGAGCCACATGTGCGTCGATGGCGTTACTCGCGCGGCGGCCGATCTGGGTTACACCG
TTACGGTGATCCACGATGCTTGCGCCAGCCGTGACCTGGAATTCAACGGTCTGACCGTGCCCGCCGCCCACGTGCATGCG
GCGTTCATGTCGGCGCTGGGGTTTGCCTATGCCAGCGTGGTATCCACCGACGAGTTCCTCGCTGCCAGCCGCTAA

Upstream 100 bases:

>100_bases
TGTCAGTTTTCCCCTGTGTATTGTCGGTGCGGCCAATCCTCGAAACCGTGGCCAGCGCCAATACTGAACCCACACCCACC
CACACTTCGAGGACACACCC

Downstream 100 bases:

>100_bases
GCCCTGCTCCGACACACCCGGAAAAATCTGCGCCCGCCACGTTGCGGGCGTTTTTTCGCGTGTCATCAAAATCTCACGCG
TGAGCCATTGATGGCACTTT

Product: isochorismatase hydrolase

Products: Nicotinate; pyrazinoic acid; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 184; Mature: 183

Protein sequence:

>184_residues
MAKQALIVVDIQNDYFPQGKWPLAGADAAADNAARLIAAFREAGDSVVHIRHEFTSPEAPFFTPGSEGAKLHPKVLNRAD
EPVVLKHFVNSFRETELQSILDEQGIKELVVVGSMSHMCVDGVTRAAADLGYTVTVIHDACASRDLEFNGLTVPAAHVHA
AFMSALGFAYASVVSTDEFLAASR

Sequences:

>Translated_184_residues
MAKQALIVVDIQNDYFPQGKWPLAGADAAADNAARLIAAFREAGDSVVHIRHEFTSPEAPFFTPGSEGAKLHPKVLNRAD
EPVVLKHFVNSFRETELQSILDEQGIKELVVVGSMSHMCVDGVTRAAADLGYTVTVIHDACASRDLEFNGLTVPAAHVHA
AFMSALGFAYASVVSTDEFLAASR
>Mature_183_residues
AKQALIVVDIQNDYFPQGKWPLAGADAAADNAARLIAAFREAGDSVVHIRHEFTSPEAPFFTPGSEGAKLHPKVLNRADE
PVVLKHFVNSFRETELQSILDEQGIKELVVVGSMSHMCVDGVTRAAADLGYTVTVIHDACASRDLEFNGLTVPAAHVHAA
FMSALGFAYASVVSTDEFLAASR

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 19751; Mature: 19620

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQALIVVDIQNDYFPQGKWPLAGADAAADNAARLIAAFREAGDSVVHIRHEFTSPEAP
CCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
FFTPGSEGAKLHPKVLNRADEPVVLKHFVNSFRETELQSILDEQGIKELVVVGSMSHMCV
EECCCCCCCEECHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCHHHHH
DGVTRAAADLGYTVTVIHDACASRDLEFNGLTVPAAHVHAAFMSALGFAYASVVSTDEFL
HHHHHHHHHCCCEEEEEEHHHHCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
AASR
HCCC
>Mature Secondary Structure 
AKQALIVVDIQNDYFPQGKWPLAGADAAADNAARLIAAFREAGDSVVHIRHEFTSPEAP
CCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
FFTPGSEGAKLHPKVLNRADEPVVLKHFVNSFRETELQSILDEQGIKELVVVGSMSHMCV
EECCCCCCCEECHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCHHHHH
DGVTRAAADLGYTVTVIHDACASRDLEFNGLTVPAAHVHAAFMSALGFAYASVVSTDEFL
HHHHHHHHHCCCEEEEEEHHHHCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
AASR
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Nicotinamide; pyrazinamide; H2O [C]

Specific reaction: Nicotinamide + H2O = Nicotinate + NH3 pyrazinamide + H2O = pyrazinoic acid + NH3 [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]