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The map label for this gene is btuF [H]

Identifier: 77461230

GI number: 77461230

Start: 5646864

End: 5647664

Strand: Reverse

Name: btuF [H]

Synonym: Pfl01_5009

Alternate gene names: 77461230

Gene position: 5647664-5646864 (Counterclockwise)

Preceding gene: 77461231

Following gene: 77461224

Centisome position: 87.72

GC content: 63.55

Gene sequence:

>801_bases
ATGCGGCGTCTCTGGCTGGCGGTTCTGCTGCTGGCCGTCAGCGGTGAAACGCTGGCCGCCCTGCGGGTGGTCAGCCTTGC
GCCATCCCTGTCTGAAATCGTTGTCGAACTGGATTCGGCCGACCTGTTGGTCGGGGTGCTGGATGGCGGTGAGCGGCCAT
CCGCGATCAAGGACGTGCCTTCGGTGGGCCGTTATGGCCAGCTTGACATGGAGCGGCTGCTCAGCCTCAAACCTGACTTG
TTGCTGTTGTGGCCCGGCAGTGTCGGCCCGGCTCAACGTGATCAGCTCAAGCGCCTGAACATCCCGACGTTCGTTGCCGA
GCCTCACAGTCTCGAACAGCTGACTGCGCAGATTGAGGCCATCGCGATACAGCTCGGTCGTCCTGAGCGTGGGGTCGAAT
GGGCTGCGGCGCTGCGGCGCAACCTTGATGAACTGCGCCAACGCTACCGCCGGGAAACGCCGCTGCGGGTGTTCTATCAG
ATCTGGGACGCGCCGCTGTACACCGTCGGCGGCGGGCAGATCATCAGCGATGCGCTTGAAGTGTGCGGCGCGCGCAATGT
GTTTGCCGATTTGAGCCTGCCCGCGCCGCAGGTGAGCATCGAGGCGGTTTTACAGCGTGATCCCGAAGTGATTCTCGCCG
GCGATCAGCCGCAACTCGATGCGTGGAACGCCTGGCCGCAGGTCACAGCGGTGAAGCATCGGCAATTACTGCTGGTCACC
GACAAAGGTCTGGAGCGCCCGAGCGGGCAGATGATCGAGGCGACCGCCAAACTCTGCCACCTGATCGCGCCAGATCGTTA
G

Upstream 100 bases:

>100_bases
TGGCGGTGTTCGTCGGCCTTGAAGCGGTGCCGCTGATGTTGCTGGCGCTGTCCACGGCGGTGCTGTTCTACCTCTACCGT
ATCCCGCGTCAACTCGCCTG

Downstream 100 bases:

>100_bases
AGCTGCGGTGTCCAGGTCACGCCGAACATGAACGCCCGACCTTCCTCGCGATAGTCATACTGACTGCCGTCATGGCTGTA
CAGCGCCCGGCTGTAACCCT

Product: periplasmic binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MRRLWLAVLLLAVSGETLAALRVVSLAPSLSEIVVELDSADLLVGVLDGGERPSAIKDVPSVGRYGQLDMERLLSLKPDL
LLLWPGSVGPAQRDQLKRLNIPTFVAEPHSLEQLTAQIEAIAIQLGRPERGVEWAAALRRNLDELRQRYRRETPLRVFYQ
IWDAPLYTVGGGQIISDALEVCGARNVFADLSLPAPQVSIEAVLQRDPEVILAGDQPQLDAWNAWPQVTAVKHRQLLLVT
DKGLERPSGQMIEATAKLCHLIAPDR

Sequences:

>Translated_266_residues
MRRLWLAVLLLAVSGETLAALRVVSLAPSLSEIVVELDSADLLVGVLDGGERPSAIKDVPSVGRYGQLDMERLLSLKPDL
LLLWPGSVGPAQRDQLKRLNIPTFVAEPHSLEQLTAQIEAIAIQLGRPERGVEWAAALRRNLDELRQRYRRETPLRVFYQ
IWDAPLYTVGGGQIISDALEVCGARNVFADLSLPAPQVSIEAVLQRDPEVILAGDQPQLDAWNAWPQVTAVKHRQLLLVT
DKGLERPSGQMIEATAKLCHLIAPDR
>Mature_266_residues
MRRLWLAVLLLAVSGETLAALRVVSLAPSLSEIVVELDSADLLVGVLDGGERPSAIKDVPSVGRYGQLDMERLLSLKPDL
LLLWPGSVGPAQRDQLKRLNIPTFVAEPHSLEQLTAQIEAIAIQLGRPERGVEWAAALRRNLDELRQRYRRETPLRVFYQ
IWDAPLYTVGGGQIISDALEVCGARNVFADLSLPAPQVSIEAVLQRDPEVILAGDQPQLDAWNAWPQVTAVKHRQLLLVT
DKGLERPSGQMIEATAKLCHLIAPDR

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786353, Length=249, Percent_Identity=30.1204819277108, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 29342; Mature: 29342

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLWLAVLLLAVSGETLAALRVVSLAPSLSEIVVELDSADLLVGVLDGGERPSAIKDVP
CHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHEECCCCCEEEEEECCCCCCCHHHCCC
SVGRYGQLDMERLLSLKPDLLLLWPGSVGPAQRDQLKRLNIPTFVAEPHSLEQLTAQIEA
CCCCCCCCCHHHHHCCCCCEEEEECCCCCCCHHHHHHHCCCCEEECCCHHHHHHHHHHHH
IAIQLGRPERGVEWAAALRRNLDELRQRYRRETPLRVFYQIWDAPLYTVGGGQIISDALE
HHEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEECCCHHHHHHHH
VCGARNVFADLSLPAPQVSIEAVLQRDPEVILAGDQPQLDAWNAWPQVTAVKHRQLLLVT
HCCCCCCEEECCCCCCHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCEEECCCEEEEEE
DKGLERPSGQMIEATAKLCHLIAPDR
CCCCCCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRRLWLAVLLLAVSGETLAALRVVSLAPSLSEIVVELDSADLLVGVLDGGERPSAIKDVP
CHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHEECCCCCEEEEEECCCCCCCHHHCCC
SVGRYGQLDMERLLSLKPDLLLLWPGSVGPAQRDQLKRLNIPTFVAEPHSLEQLTAQIEA
CCCCCCCCCHHHHHCCCCCEEEEECCCCCCCHHHHHHHCCCCEEECCCHHHHHHHHHHHH
IAIQLGRPERGVEWAAALRRNLDELRQRYRRETPLRVFYQIWDAPLYTVGGGQIISDALE
HHEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEECCCHHHHHHHH
VCGARNVFADLSLPAPQVSIEAVLQRDPEVILAGDQPQLDAWNAWPQVTAVKHRQLLLVT
HCCCCCCEEECCCCCCHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCEEECCCEEEEEE
DKGLERPSGQMIEATAKLCHLIAPDR
CCCCCCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA