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The map label for this gene is eutB [H]

Identifier: 77461212

GI number: 77461212

Start: 5623220

End: 5624614

Strand: Direct

Name: eutB [H]

Synonym: Pfl01_4991

Alternate gene names: 77461212

Gene position: 5623220-5624614 (Clockwise)

Preceding gene: 77461211

Following gene: 77461213

Centisome position: 87.34

GC content: 62.94

Gene sequence:

>1395_bases
ATGGCCGCATTTGCCCATACCGTCGGCGCCCAGACCTACCGCTTCGACAGCCTCAAGGACGTGATGGCCAAGGCCAGCCC
GGCACGTTCCGGGGATTTCCTGGCCGGCGTCGCCGCGCTCAACGATGGCGAGCGGGTGGCCGCGCAAATGGCGCTGGCCG
ACATCCCGCTCACGCATTTCCTGCAGGAAGCGCTGATTCCCTACGAAGCCGATGAAGTCACCCGGCTGATCATCGACAGC
CATGACAAACAGGCCTTCGCAGTCGTCAGCCATCTCACGGTTGGCGGCTTTCGCGACTGGTTGTTGAGCGAAGCGGCCGA
TGAATCCAGCCTGCGTGCCCTTGCCCCCGGCCTGACGCCGGAAATGGTCGCTGCCGTGTCGAAGATCATGCGCGTGCAGG
ATCTGGTGCTGGTGGCGCAGAAGATCCGCGTCGTCACGAAGTTTCGCGGCACCCTCGGTCTGCGCGGGCGCTTATCCACA
AGGCTGCAACCCAATCACCCGACCGACGAGCCGTCCGGCATCGCCGCAAGCATTCTCGACGGCCTGCTGTACGGCAACGG
CGACGCGATGATCGGCATTAACCCGGCCACCGACAGCATCGCCTCGATCTGCGCGATGCTGGAAATGCTCGACGCGATTA
TCCAGCGCTACGACATTCCGACCCAGGCCTGCGTGCTGACCCACGTCACCACGTCCATAGAAGCGATCAACCGTGGCGTG
CCGCTGGATCTGGTGTTCCAGTCGATTGCCGGCACCGAAGCGGCCAACGCCAGTTTCGGCATCAACCTGAACATTTTGCA
GGAAGGTTATGAAGCGGGCCTGAGCCTCAATCGCGGTACGCTCGGGCAGAACCTGATGTATTTCGAAACCGGTCAGGGCA
GTGCGCTGTCAGCCAACGCTCACCACGGCGTCGATCAACAGACCTGCGAGACCCGGGCCTACGCCGTGGCGCGCCATTTC
AAGCCATTTCTGGTGAACACCGTCGTAGGATTCATCGGCCCGGAATACCTCTACAACGGCAAGCAGATCATCCGCGCCGG
CCTCGAAGACCACTTCTGCGGCAAGCTGCTCGGCGTGCCGATGGGCTGCGACATCTGCTACACCAACCACGCCGAAGCCG
ACCAGGATGACATGGACACCCTGCTCACCTTGCTGGGCGTGGCCGGGATCAACTTCATCATGGGCATCCCCGGCTCCGAC
GACATCATGCTCAACTACCAGACCACCTCGTTCCACGACGCGCTCTACGCGCGCCAGACCCTGGGCCTGAAACCGGCGCC
GGAATTCGAACAGTGGCTGGCGAAGATGGGCATCTTCACCCAGGCGGACGGCAAGGTCCGCTTTGGCAACAACCTGCCGC
CGGCCTTCCGCCAGGCGTTGGCGCAACTGGGATGA

Upstream 100 bases:

>100_bases
CTATGCTCGAAGGTATCTAGAAGTTATCCGAGCTGCACCGGCACCGGGCTTCGTGTCCGGTGCCTGCACTAAAAGCATTC
AATCAGGAGGAACGCGCCCC

Downstream 100 bases:

>100_bases
GTCAGATGGAAAAACCGCCCGTCGATCCGCAAAACCCGTGGCTGGAGCTGCGTCGTCTGACCCCGGCGCGCATTGCCCTC
GGTCGTACCGGCACCAGTCT

Product: ethanolamine ammonia-lyase heavy chain

Products: NA

Alternate protein names: Ethanolamine ammonia-lyase large subunit [H]

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MAAFAHTVGAQTYRFDSLKDVMAKASPARSGDFLAGVAALNDGERVAAQMALADIPLTHFLQEALIPYEADEVTRLIIDS
HDKQAFAVVSHLTVGGFRDWLLSEAADESSLRALAPGLTPEMVAAVSKIMRVQDLVLVAQKIRVVTKFRGTLGLRGRLST
RLQPNHPTDEPSGIAASILDGLLYGNGDAMIGINPATDSIASICAMLEMLDAIIQRYDIPTQACVLTHVTTSIEAINRGV
PLDLVFQSIAGTEAANASFGINLNILQEGYEAGLSLNRGTLGQNLMYFETGQGSALSANAHHGVDQQTCETRAYAVARHF
KPFLVNTVVGFIGPEYLYNGKQIIRAGLEDHFCGKLLGVPMGCDICYTNHAEADQDDMDTLLTLLGVAGINFIMGIPGSD
DIMLNYQTTSFHDALYARQTLGLKPAPEFEQWLAKMGIFTQADGKVRFGNNLPPAFRQALAQLG

Sequences:

>Translated_464_residues
MAAFAHTVGAQTYRFDSLKDVMAKASPARSGDFLAGVAALNDGERVAAQMALADIPLTHFLQEALIPYEADEVTRLIIDS
HDKQAFAVVSHLTVGGFRDWLLSEAADESSLRALAPGLTPEMVAAVSKIMRVQDLVLVAQKIRVVTKFRGTLGLRGRLST
RLQPNHPTDEPSGIAASILDGLLYGNGDAMIGINPATDSIASICAMLEMLDAIIQRYDIPTQACVLTHVTTSIEAINRGV
PLDLVFQSIAGTEAANASFGINLNILQEGYEAGLSLNRGTLGQNLMYFETGQGSALSANAHHGVDQQTCETRAYAVARHF
KPFLVNTVVGFIGPEYLYNGKQIIRAGLEDHFCGKLLGVPMGCDICYTNHAEADQDDMDTLLTLLGVAGINFIMGIPGSD
DIMLNYQTTSFHDALYARQTLGLKPAPEFEQWLAKMGIFTQADGKVRFGNNLPPAFRQALAQLG
>Mature_463_residues
AAFAHTVGAQTYRFDSLKDVMAKASPARSGDFLAGVAALNDGERVAAQMALADIPLTHFLQEALIPYEADEVTRLIIDSH
DKQAFAVVSHLTVGGFRDWLLSEAADESSLRALAPGLTPEMVAAVSKIMRVQDLVLVAQKIRVVTKFRGTLGLRGRLSTR
LQPNHPTDEPSGIAASILDGLLYGNGDAMIGINPATDSIASICAMLEMLDAIIQRYDIPTQACVLTHVTTSIEAINRGVP
LDLVFQSIAGTEAANASFGINLNILQEGYEAGLSLNRGTLGQNLMYFETGQGSALSANAHHGVDQQTCETRAYAVARHFK
PFLVNTVVGFIGPEYLYNGKQIIRAGLEDHFCGKLLGVPMGCDICYTNHAEADQDDMDTLLTLLGVAGINFIMGIPGSDD
IMLNYQTTSFHDALYARQTLGLKPAPEFEQWLAKMGIFTQADGKVRFGNNLPPAFRQALAQLG

Specific function: Ethanolamine utilization. [C]

COG id: COG4303

COG function: function code E; Ethanolamine ammonia-lyase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082106, Length=447, Percent_Identity=51.006711409396, Blast_Score=453, Evalue=1e-129,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010628 [H]

Pfam domain/function: PF06751 EutB [H]

EC number: =4.3.1.7 [H]

Molecular weight: Translated: 49958; Mature: 49827

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAFAHTVGAQTYRFDSLKDVMAKASPARSGDFLAGVAALNDGERVAAQMALADIPLTHF
CCCHHHHHCCCCEEHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCHHHH
LQEALIPYEADEVTRLIIDSHDKQAFAVVSHLTVGGFRDWLLSEAADESSLRALAPGLTP
HHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCH
EMVAAVSKIMRVQDLVLVAQKIRVVTKFRGTLGLRGRLSTRLQPNHPTDEPSGIAASILD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
GLLYGNGDAMIGINPATDSIASICAMLEMLDAIIQRYDIPTQACVLTHVTTSIEAINRGV
HHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
PLDLVFQSIAGTEAANASFGINLNILQEGYEAGLSLNRGTLGQNLMYFETGQGSALSANA
CHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCEEECCC
HHGVDQQTCETRAYAVARHFKPFLVNTVVGFIGPEYLYNGKQIIRAGLEDHFCGKLLGVP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCHHHHHHCCCHHHHHHHHCCC
MGCDICYTNHAEADQDDMDTLLTLLGVAGINFIMGIPGSDDIMLNYQTTSFHDALYARQT
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEEECCHHHHHHHHHHH
LGLKPAPEFEQWLAKMGIFTQADGKVRFGNNLPPAFRQALAQLG
CCCCCCCHHHHHHHHHCCEEECCCEEEECCCCCHHHHHHHHHCC
>Mature Secondary Structure 
AAFAHTVGAQTYRFDSLKDVMAKASPARSGDFLAGVAALNDGERVAAQMALADIPLTHF
CCHHHHHCCCCEEHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCHHHH
LQEALIPYEADEVTRLIIDSHDKQAFAVVSHLTVGGFRDWLLSEAADESSLRALAPGLTP
HHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCH
EMVAAVSKIMRVQDLVLVAQKIRVVTKFRGTLGLRGRLSTRLQPNHPTDEPSGIAASILD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
GLLYGNGDAMIGINPATDSIASICAMLEMLDAIIQRYDIPTQACVLTHVTTSIEAINRGV
HHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
PLDLVFQSIAGTEAANASFGINLNILQEGYEAGLSLNRGTLGQNLMYFETGQGSALSANA
CHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCEEECCC
HHGVDQQTCETRAYAVARHFKPFLVNTVVGFIGPEYLYNGKQIIRAGLEDHFCGKLLGVP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCHHHHHHCCCHHHHHHHHCCC
MGCDICYTNHAEADQDDMDTLLTLLGVAGINFIMGIPGSDDIMLNYQTTSFHDALYARQT
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEEECCHHHHHHHHHHH
LGLKPAPEFEQWLAKMGIFTQADGKVRFGNNLPPAFRQALAQLG
CCCCCCCHHHHHHHHHCCEEECCCEEEECCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]