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The map label for this gene is mltB [H]

Identifier: 77461182

GI number: 77461182

Start: 5589057

End: 5590379

Strand: Direct

Name: mltB [H]

Synonym: Pfl01_4961

Alternate gene names: 77461182

Gene position: 5589057-5590379 (Clockwise)

Preceding gene: 77461181

Following gene: 77461196

Centisome position: 86.81

GC content: 63.95

Gene sequence:

>1323_bases
ATGCCCCTCAGTCTTTCCCGTCGTTGGCCCTTCCGCCAACTGATCGCTGCCTCCAGCTTCGTACTGCTCGTCGCCTGCGC
GGAAAAACCCACCGCCGCCGACGCTCAACCGCTTCAGACCGCCCCCGTTGCCACAGCCCCAGCGATCCTCCCGCCGGTAG
TGCCGTCCGGTGACAACCTCGACCTGCAACCGACCCAGACCTTTGCCGAATGGCAGGCCGGTTTCCGCAAGGAAGCTCTG
GCCGCCGGCATCCGTGCCGACCTGTTTGACCGCGCATTTGCCAATGTCAGCTTCGATGCCAGCGTGATTCGCGCCGACCG
CAGCCAGCCGGAATTCGCCCGGCCCGTCTGGGAATACCTGGACGGTGCCCTTTCGCCCTTGCGCGTGCGCAAAGGCCAGG
CCCTGATCAGCCAGTACGCCGATATCCTGCAAAGCATCGAGCAGCGCTATGGTGTCGATCGCCAGGCGCTGGTGTCGGTG
TGGGGGATGGAAAGCAACTTCGGCCAGTTCCAGGGCAGCAAGTCGGTGATCAATTCGCTGGCCACTCTGGCCTATGAAGG
CCGCCGCCCGGGCTTTGCCCATGCGCAGTTGATTGCGGCGTTGCAGATCCTGCAACAGGGCGACATCACGCCGGAAAAAA
TGCTCGGTTCCTGGGCTGGCGCCATGGGCCAGACCCAGTTCATCCCGACCACCTACAACACCCACGCCGTGGACTTCGAC
GGTGACGGTCGCCGCGACATCTGGGGCAGCCCGGCTGACGCTCTGGCATCCACCGCGCATTACCTGCAAAGCTCCGGCTG
GCAGCGTGGCCAGCCGTGGGGCTTCGAAGTCCAGCTACCAAGCGGTTTCAACTACACCCTGGCCGACGGCGCGATTCGTA
AAAGCGTTGCTGAATGGCGGCAACTGGGCGTGATCCTGCCGAACGGAGGCCAGGTTCCAGCCGGTTCCGAGCAACTGTCC
GCTGCCCTGCTGCTGCCGGCCGGCTATCGCGGACCGGCGTTCCTGATCCTCGACAACTTCCGGGCGATCCTCAAGTACAA
CAACTCGTCGTCCTACGCACTGGCCGTCAGCCTGTTGTCCGAGCGCTTCAACGGTGCCGGCCTGATCAATGGCACCTGGC
CAAAGGATGACCTGCCGCTGAGCCGCACTGAGCGGATCGAACTGCAAAACCTGCTCACTGCGCGCAACTACGACGCCGGT
GCGGCAGACGGGATCATCGGCGCCAATACGCGCAAGGCGATCCGCAGTGCGCAGCAGTCATTCGGCTGGCCGGCGGACGG
TTATCCGACGCACAAACTGCTGGAAAGCCTGCGTAACCAGTAA

Upstream 100 bases:

>100_bases
TCTGACAGCTGGGAGGCTTCTTACTTTCTGGCCGCTTGAAGCACAGCACCCCTTCCTCATGTTAAGGTCTGCACCTGATT
CGTATTCCCTGGACCTGTGC

Downstream 100 bases:

>100_bases
AGACAAAAGACCGCCGCCTCTTCAGGCGGCGGTCTTTTTTTGTGGCTCGATCAGCGAATCACTACATCCTGTTCCAGCAT
CAATTCTTTCTTCCCTGCAT

Product: lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MPLSLSRRWPFRQLIAASSFVLLVACAEKPTAADAQPLQTAPVATAPAILPPVVPSGDNLDLQPTQTFAEWQAGFRKEAL
AAGIRADLFDRAFANVSFDASVIRADRSQPEFARPVWEYLDGALSPLRVRKGQALISQYADILQSIEQRYGVDRQALVSV
WGMESNFGQFQGSKSVINSLATLAYEGRRPGFAHAQLIAALQILQQGDITPEKMLGSWAGAMGQTQFIPTTYNTHAVDFD
GDGRRDIWGSPADALASTAHYLQSSGWQRGQPWGFEVQLPSGFNYTLADGAIRKSVAEWRQLGVILPNGGQVPAGSEQLS
AALLLPAGYRGPAFLILDNFRAILKYNNSSSYALAVSLLSERFNGAGLINGTWPKDDLPLSRTERIELQNLLTARNYDAG
AADGIIGANTRKAIRSAQQSFGWPADGYPTHKLLESLRNQ

Sequences:

>Translated_440_residues
MPLSLSRRWPFRQLIAASSFVLLVACAEKPTAADAQPLQTAPVATAPAILPPVVPSGDNLDLQPTQTFAEWQAGFRKEAL
AAGIRADLFDRAFANVSFDASVIRADRSQPEFARPVWEYLDGALSPLRVRKGQALISQYADILQSIEQRYGVDRQALVSV
WGMESNFGQFQGSKSVINSLATLAYEGRRPGFAHAQLIAALQILQQGDITPEKMLGSWAGAMGQTQFIPTTYNTHAVDFD
GDGRRDIWGSPADALASTAHYLQSSGWQRGQPWGFEVQLPSGFNYTLADGAIRKSVAEWRQLGVILPNGGQVPAGSEQLS
AALLLPAGYRGPAFLILDNFRAILKYNNSSSYALAVSLLSERFNGAGLINGTWPKDDLPLSRTERIELQNLLTARNYDAG
AADGIIGANTRKAIRSAQQSFGWPADGYPTHKLLESLRNQ
>Mature_439_residues
PLSLSRRWPFRQLIAASSFVLLVACAEKPTAADAQPLQTAPVATAPAILPPVVPSGDNLDLQPTQTFAEWQAGFRKEALA
AGIRADLFDRAFANVSFDASVIRADRSQPEFARPVWEYLDGALSPLRVRKGQALISQYADILQSIEQRYGVDRQALVSVW
GMESNFGQFQGSKSVINSLATLAYEGRRPGFAHAQLIAALQILQQGDITPEKMLGSWAGAMGQTQFIPTTYNTHAVDFDG
DGRRDIWGSPADALASTAHYLQSSGWQRGQPWGFEVQLPSGFNYTLADGAIRKSVAEWRQLGVILPNGGQVPAGSEQLSA
ALLLPAGYRGPAFLILDNFRAILKYNNSSSYALAVSLLSERFNGAGLINGTWPKDDLPLSRTERIELQNLLTARNYDAGA
ADGIIGANTRKAIRSAQQSFGWPADGYPTHKLLESLRNQ

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=360, Percent_Identity=29.7222222222222, Blast_Score=125, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 47831; Mature: 47700

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLSLSRRWPFRQLIAASSFVLLVACAEKPTAADAQPLQTAPVATAPAILPPVVPSGDNL
CCCCCCCCCCHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DLQPTQTFAEWQAGFRKEALAAGIRADLFDRAFANVSFDASVIRADRSQPEFARPVWEYL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHHHH
DGALSPLRVRKGQALISQYADILQSIEQRYGVDRQALVSVWGMESNFGQFQGSKSVINSL
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHCCCHHHHHHHH
ATLAYEGRRPGFAHAQLIAALQILQQGDITPEKMLGSWAGAMGQTQFIPTTYNTHAVDFD
HHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEECCCCCCEEEECC
GDGRRDIWGSPADALASTAHYLQSSGWQRGQPWGFEVQLPSGFNYTLADGAIRKSVAEWR
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCEECCCHHHHHHHHHHH
QLGVILPNGGQVPAGSEQLSAALLLPAGYRGPAFLILDNFRAILKYNNSSSYALAVSLLS
HCCEECCCCCCCCCCHHHHCEEEEECCCCCCCEEEEECCCEEEEEECCCCCHHHHHHHHH
ERFNGAGLINGTWPKDDLPLSRTERIELQNLLTARNYDAGAADGIIGANTRKAIRSAQQS
HHCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
FGWPADGYPTHKLLESLRNQ
CCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
PLSLSRRWPFRQLIAASSFVLLVACAEKPTAADAQPLQTAPVATAPAILPPVVPSGDNL
CCCCCCCCCHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DLQPTQTFAEWQAGFRKEALAAGIRADLFDRAFANVSFDASVIRADRSQPEFARPVWEYL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHHHH
DGALSPLRVRKGQALISQYADILQSIEQRYGVDRQALVSVWGMESNFGQFQGSKSVINSL
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHCCCHHHHHHHH
ATLAYEGRRPGFAHAQLIAALQILQQGDITPEKMLGSWAGAMGQTQFIPTTYNTHAVDFD
HHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEECCCCCCEEEECC
GDGRRDIWGSPADALASTAHYLQSSGWQRGQPWGFEVQLPSGFNYTLADGAIRKSVAEWR
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCEECCCHHHHHHHHHHH
QLGVILPNGGQVPAGSEQLSAALLLPAGYRGPAFLILDNFRAILKYNNSSSYALAVSLLS
HCCEECCCCCCCCCCHHHHCEEEEECCCCCCCEEEEECCCEEEEEECCCCCHHHHHHHHH
ERFNGAGLINGTWPKDDLPLSRTERIELQNLLTARNYDAGAADGIIGANTRKAIRSAQQS
HHCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
FGWPADGYPTHKLLESLRNQ
CCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]