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The map label for this gene is miaB [H]

Identifier: 77461171

GI number: 77461171

Start: 5577486

End: 5578814

Strand: Direct

Name: miaB [H]

Synonym: Pfl01_4950

Alternate gene names: 77461171

Gene position: 5577486-5578814 (Clockwise)

Preceding gene: 77461169

Following gene: 77461172

Centisome position: 86.63

GC content: 62.75

Gene sequence:

>1329_bases
ATGGCCAAGAAGCTTTACATCGAAACCCACGGTTGCCAGATGAACGAGTACGACAGCTCGCGCATGGTCGATCTGCTGGG
CGAACACCAGGCCCTGGAAGTCACGGCGCGCGCCGAAGATGCCGACGTGATCCTGCTCAACACCTGCTCGATCCGCGAAC
GCGCCCAGGACCGGGTGTATTCCCAGCTCGGCCGCTGGCGCGAACTGAAACTGGCCAACCCGGACATGGTGATCGCCGTC
GGCGGTTGCGTGGCCAGCCAGGAAGGCGCGGCGATCCGCGATCGCGCGCCGTACGTCGACGTGGTGTTCGGCCCGCAGAC
CCTGCACCGTCTGCCGGAAATGATCGACGCCGCGCGCATCAGCAAGCTGCCGCAAGTCGACGTGTCGTTCCCGGAAATCG
AAAAATTCGACCACCTGCCCGAGCCGCGTATCGACGGCCCGAGCGCCTATGTGTCGGTGATGGAGGGCTGCAGCAAGTAC
TGCACGTTCTGCGTGGTGCCTTACACCCGCGGCGAAGAAGTCAGCCGACCGTTCGACGACGTGATCGCCGAGATCATCCA
CCTGGCCGAAAACGGTGTGCGTGAAGTGACCCTGCTGGGGCAGAACGTCAACGGCTATCGCGGGCTGACCCACGACGGTC
GCCTGGCGGACCTGGCCGAACTGATCCGCGTGGTCGCGGCGGTCGACGGCATCGACCGCATCCGCTACACCACTTCGCAC
CCGCTGGAGTTCTCCGACAGCCTGATCCAGGCCCACGCCGAAGTGCCGGAACTGGTCAAGCATCTGCACTTGCCGGTGCA
GTCGGGTTCGGACCGGATCCTGGCCGCGATGAAGCGCAACCACACGGCGCTGGAATACAAATCCAAGCTGCGCAAACTGC
GTGCCGCCGTACCGGGCATCTGCATCAGTTCGGACTTCATCGTCGGCTTCCCGGGGGAAACCGAGAAAGATTTCGAACAG
ACCATGAAGCTGATTGCCGACGTCGGTTTCGACTTTTCCTACTCGTTCGTTTACAGCCAACGCCCGGGCACCCCGGCTGC
CGACCTGGCCGACGACACCCCGGAAGAGCTGAAGAAAGAGCGCCTGAACGCCTTGCAGCACCGTTTGAACCAGCAAGGGT
TCGAGATCAGCCGACAAATGGTCGGTTCGGTCCAGCGGATTCTGGTGACCGATTATTCGAAGAAAGACCCCGGCGAGCTG
CAAGGGCGGACCGAGAATAACCGTATCGTCAACTTCCGCTGCGATAATCCGACCCTGATCGGCCAGTTCGCCGACGTCCA
CATCGACGCGGCGCAACCGCACTCGCTGCGCGGCTCGCTGATCCAGTAA

Upstream 100 bases:

>100_bases
GCGCGAACTATAGCATAGCCACCGGACGCGCAGCGGTAGGAACCTGGGGACATGTTCCCTATAATGCGCGGTTCTTTGAA
CCACGTGACGACCCTATTCC

Downstream 100 bases:

>100_bases
CACCGCAAGGAGACCGCGCCAGACGGCTGAATCGATGCAAGGCTTTTTAAGAGCTTTCGCACCCACGCCGCTGGCGTTAT
CCTTGATTTCATCCTAATTG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MAKKLYIETHGCQMNEYDSSRMVDLLGEHQALEVTARAEDADVILLNTCSIRERAQDRVYSQLGRWRELKLANPDMVIAV
GGCVASQEGAAIRDRAPYVDVVFGPQTLHRLPEMIDAARISKLPQVDVSFPEIEKFDHLPEPRIDGPSAYVSVMEGCSKY
CTFCVVPYTRGEEVSRPFDDVIAEIIHLAENGVREVTLLGQNVNGYRGLTHDGRLADLAELIRVVAAVDGIDRIRYTTSH
PLEFSDSLIQAHAEVPELVKHLHLPVQSGSDRILAAMKRNHTALEYKSKLRKLRAAVPGICISSDFIVGFPGETEKDFEQ
TMKLIADVGFDFSYSFVYSQRPGTPAADLADDTPEELKKERLNALQHRLNQQGFEISRQMVGSVQRILVTDYSKKDPGEL
QGRTENNRIVNFRCDNPTLIGQFADVHIDAAQPHSLRGSLIQ

Sequences:

>Translated_442_residues
MAKKLYIETHGCQMNEYDSSRMVDLLGEHQALEVTARAEDADVILLNTCSIRERAQDRVYSQLGRWRELKLANPDMVIAV
GGCVASQEGAAIRDRAPYVDVVFGPQTLHRLPEMIDAARISKLPQVDVSFPEIEKFDHLPEPRIDGPSAYVSVMEGCSKY
CTFCVVPYTRGEEVSRPFDDVIAEIIHLAENGVREVTLLGQNVNGYRGLTHDGRLADLAELIRVVAAVDGIDRIRYTTSH
PLEFSDSLIQAHAEVPELVKHLHLPVQSGSDRILAAMKRNHTALEYKSKLRKLRAAVPGICISSDFIVGFPGETEKDFEQ
TMKLIADVGFDFSYSFVYSQRPGTPAADLADDTPEELKKERLNALQHRLNQQGFEISRQMVGSVQRILVTDYSKKDPGEL
QGRTENNRIVNFRCDNPTLIGQFADVHIDAAQPHSLRGSLIQ
>Mature_441_residues
AKKLYIETHGCQMNEYDSSRMVDLLGEHQALEVTARAEDADVILLNTCSIRERAQDRVYSQLGRWRELKLANPDMVIAVG
GCVASQEGAAIRDRAPYVDVVFGPQTLHRLPEMIDAARISKLPQVDVSFPEIEKFDHLPEPRIDGPSAYVSVMEGCSKYC
TFCVVPYTRGEEVSRPFDDVIAEIIHLAENGVREVTLLGQNVNGYRGLTHDGRLADLAELIRVVAAVDGIDRIRYTTSHP
LEFSDSLIQAHAEVPELVKHLHLPVQSGSDRILAAMKRNHTALEYKSKLRKLRAAVPGICISSDFIVGFPGETEKDFEQT
MKLIADVGFDFSYSFVYSQRPGTPAADLADDTPEELKKERLNALQHRLNQQGFEISRQMVGSVQRILVTDYSKKDPGELQ
GRTENNRIVNFRCDNPTLIGQFADVHIDAAQPHSLRGSLIQ

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=478, Percent_Identity=35.5648535564854, Blast_Score=276, Evalue=3e-74,
Organism=Homo sapiens, GI28872782, Length=478, Percent_Identity=35.5648535564854, Blast_Score=275, Evalue=6e-74,
Organism=Homo sapiens, GI93277076, Length=456, Percent_Identity=27.6315789473684, Blast_Score=161, Evalue=1e-39,
Organism=Escherichia coli, GI1786882, Length=442, Percent_Identity=65.8371040723982, Blast_Score=619, Evalue=1e-178,
Organism=Escherichia coli, GI1787057, Length=455, Percent_Identity=27.4725274725275, Blast_Score=158, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI17553146, Length=464, Percent_Identity=33.6206896551724, Blast_Score=247, Evalue=9e-66,
Organism=Caenorhabditis elegans, GI71996771, Length=259, Percent_Identity=32.046332046332, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI21356207, Length=434, Percent_Identity=34.331797235023, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI19922432, Length=454, Percent_Identity=28.4140969162996, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 49396; Mature: 49264

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKLYIETHGCQMNEYDSSRMVDLLGEHQALEVTARAEDADVILLNTCSIRERAQDRVY
CCCEEEEEECCCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCEEEEECCCHHHHHHHHHH
SQLGRWRELKLANPDMVIAVGGCVASQEGAAIRDRAPYVDVVFGPQTLHRLPEMIDAARI
HHHCCCEEEEECCCCEEEEECCEEECCCCCEECCCCCEEEEEECHHHHHHHHHHHHHHHH
SKLPQVDVSFPEIEKFDHLPEPRIDGPSAYVSVMEGCSKYCTFCVVPYTRGEEVSRPFDD
HCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCCCHHCCCHHH
VIAEIIHLAENGVREVTLLGQNVNGYRGLTHDGRLADLAELIRVVAAVDGIDRIRYTTSH
HHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHEECCCC
PLEFSDSLIQAHAEVPELVKHLHLPVQSGSDRILAAMKRNHTALEYKSKLRKLRAAVPGI
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCE
CISSDFIVGFPGETEKDFEQTMKLIADVGFDFSYSFVYSQRPGTPAADLADDTPEELKKE
EECCCEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHH
RLNALQHRLNQQGFEISRQMVGSVQRILVTDYSKKDPGELQGRTENNRIVNFRCDNPTLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCEEE
GQFADVHIDAAQPHSLRGSLIQ
EEEEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
AKKLYIETHGCQMNEYDSSRMVDLLGEHQALEVTARAEDADVILLNTCSIRERAQDRVY
CCEEEEEECCCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCEEEEECCCHHHHHHHHHH
SQLGRWRELKLANPDMVIAVGGCVASQEGAAIRDRAPYVDVVFGPQTLHRLPEMIDAARI
HHHCCCEEEEECCCCEEEEECCEEECCCCCEECCCCCEEEEEECHHHHHHHHHHHHHHHH
SKLPQVDVSFPEIEKFDHLPEPRIDGPSAYVSVMEGCSKYCTFCVVPYTRGEEVSRPFDD
HCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCCCHHCCCHHH
VIAEIIHLAENGVREVTLLGQNVNGYRGLTHDGRLADLAELIRVVAAVDGIDRIRYTTSH
HHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHEECCCC
PLEFSDSLIQAHAEVPELVKHLHLPVQSGSDRILAAMKRNHTALEYKSKLRKLRAAVPGI
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCE
CISSDFIVGFPGETEKDFEQTMKLIADVGFDFSYSFVYSQRPGTPAADLADDTPEELKKE
EECCCEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHH
RLNALQHRLNQQGFEISRQMVGSVQRILVTDYSKKDPGELQGRTENNRIVNFRCDNPTLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCEEE
GQFADVHIDAAQPHSLRGSLIQ
EEEEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA