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The map label for this gene is hemL [H]

Identifier: 77461168

GI number: 77461168

Start: 5574861

End: 5576144

Strand: Direct

Name: hemL [H]

Synonym: Pfl01_4947

Alternate gene names: 77461168

Gene position: 5574861-5576144 (Clockwise)

Preceding gene: 77461167

Following gene: 77461169

Centisome position: 86.59

GC content: 64.02

Gene sequence:

>1284_bases
ATGTCTCGTTCCGAAACCCTGTTTGCCAACGCCCAGAAACACATTCCCGGTGGCGTGAACTCGCCGGTACGCGCGTTCAA
GAGCGTTGGCGGCACGCCGCTGTTCTTCAAGCACGCCGAAGGCGCCTACGTCACTGACGAAGACGACAAGCGTTATGTGG
ATTACGTCGGCTCGTGGGGCCCGATGATCCTCGGCCACAGCCACCCGGACGTGCTGGACGCGGTGCGCAACCAGTTGCAG
CACGGCCTGTCCTACGGCGCGCCGACCGCGATGGAAACCGAGATGGCCGACCTGGTCTGCTCGCTGGTGCCGTCGCTGGA
GATGGTGCGCATGGTCAGCTCCGGCACCGAAGCGACCATGAGTGCAATCCGTCTGGCCCGTGGCTTCACCGGCCGCGACA
GCATCATCAAGTTCGAAGGCTGCTACCACGGTCACTCCGACAGCCTGCTGGTCAAGGCCGGTTCCGGTGCACTGACCCAA
GGCGTACCAAGCTCTGCCGGCGTACCCGCAGCGTTCGCCAAACACACCCTGACCCTGCCGTTCAACGACATCGACGCCGT
TGAAAAAATGCTCGCCGAAGTCGGCCAGGACGTGGCGTGCATCATCGTCGAGCCCGTGGCCGGCAACATGAACTGCGTAC
CGCCGGCACCGGGTTTCCTCGAAGGCCTGCGCAGCCTGTGCGACAAGCACGGCGTGGTGCTGATTTTCGACGAAGTGATG
ACTGGTTTCCGCGTCGCCCTCGGCGGCGCCCAGGCTTACTACGGCGTAACCCCGGACCTGACCACCTTCGGCAAGATCAT
CGGCGGCGGCATGCCGGTCGGTTGCTTCGGCGGCAAGCGCTTGATCATGGAGCGCATCGCGCCGTTGGGCCCGGTCTATC
AGGCGGGCACCTTGTCGGGTAACCCGCTGGCGATGGCTGCCGGCCTGACCACCCTGCGTCTGATCAGCCGTCCGGGCTTC
CACGCCGAGCTGACCGACTACACCACGCGCCTGCTCGACGGCCTGCAACAGCGCGCCGATGCCGCCGGCATTCCGTTCGT
GACCACCCAGGCGGGCGGCATGTTCGGTCTGTACTTCAGTGGTGCCGACGACATTGTTACTTTTGAAGACGTGATGGCCA
GCGATGCGGCGCTGTTCGGTCGCTTCTTCCACCTGATGCTGGAAGGTGGCGTATATCTCGCACCGAGCGCTTTCGAAGCC
GGTTTCACCTCGATCGCCCATGGCGAAGCCGAGCTGAAACTGACCCTCGACGCCGCCGAACGCGCCTTCGCCAAGTTGAA
GTAA

Upstream 100 bases:

>100_bases
CTGGCCGTGGTCCATGGCCTGTTCGGCGCCAACAGCACCGCCGAAGTGACCCGCCGCGCCCGCGCCTTCAATGAATTACT
AAAAGTCTGAGAGCCCGATC

Downstream 100 bases:

>100_bases
AACGCCCCGCCGCTGACGTTGGCCATTGCCAGCGTCAGCGTTCTCCTACATTCGCGCGCCTTTTCCGACACGCCTGCCAA
AAATCTCCCATAAAGTGGGC

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MSRSETLFANAQKHIPGGVNSPVRAFKSVGGTPLFFKHAEGAYVTDEDDKRYVDYVGSWGPMILGHSHPDVLDAVRNQLQ
HGLSYGAPTAMETEMADLVCSLVPSLEMVRMVSSGTEATMSAIRLARGFTGRDSIIKFEGCYHGHSDSLLVKAGSGALTQ
GVPSSAGVPAAFAKHTLTLPFNDIDAVEKMLAEVGQDVACIIVEPVAGNMNCVPPAPGFLEGLRSLCDKHGVVLIFDEVM
TGFRVALGGAQAYYGVTPDLTTFGKIIGGGMPVGCFGGKRLIMERIAPLGPVYQAGTLSGNPLAMAAGLTTLRLISRPGF
HAELTDYTTRLLDGLQQRADAAGIPFVTTQAGGMFGLYFSGADDIVTFEDVMASDAALFGRFFHLMLEGGVYLAPSAFEA
GFTSIAHGEAELKLTLDAAERAFAKLK

Sequences:

>Translated_427_residues
MSRSETLFANAQKHIPGGVNSPVRAFKSVGGTPLFFKHAEGAYVTDEDDKRYVDYVGSWGPMILGHSHPDVLDAVRNQLQ
HGLSYGAPTAMETEMADLVCSLVPSLEMVRMVSSGTEATMSAIRLARGFTGRDSIIKFEGCYHGHSDSLLVKAGSGALTQ
GVPSSAGVPAAFAKHTLTLPFNDIDAVEKMLAEVGQDVACIIVEPVAGNMNCVPPAPGFLEGLRSLCDKHGVVLIFDEVM
TGFRVALGGAQAYYGVTPDLTTFGKIIGGGMPVGCFGGKRLIMERIAPLGPVYQAGTLSGNPLAMAAGLTTLRLISRPGF
HAELTDYTTRLLDGLQQRADAAGIPFVTTQAGGMFGLYFSGADDIVTFEDVMASDAALFGRFFHLMLEGGVYLAPSAFEA
GFTSIAHGEAELKLTLDAAERAFAKLK
>Mature_426_residues
SRSETLFANAQKHIPGGVNSPVRAFKSVGGTPLFFKHAEGAYVTDEDDKRYVDYVGSWGPMILGHSHPDVLDAVRNQLQH
GLSYGAPTAMETEMADLVCSLVPSLEMVRMVSSGTEATMSAIRLARGFTGRDSIIKFEGCYHGHSDSLLVKAGSGALTQG
VPSSAGVPAAFAKHTLTLPFNDIDAVEKMLAEVGQDVACIIVEPVAGNMNCVPPAPGFLEGLRSLCDKHGVVLIFDEVMT
GFRVALGGAQAYYGVTPDLTTFGKIIGGGMPVGCFGGKRLIMERIAPLGPVYQAGTLSGNPLAMAAGLTTLRLISRPGFH
AELTDYTTRLLDGLQQRADAAGIPFVTTQAGGMFGLYFSGADDIVTFEDVMASDAALFGRFFHLMLEGGVYLAPSAFEAG
FTSIAHGEAELKLTLDAAERAFAKLK

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=290, Percent_Identity=28.2758620689655, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI13994255, Length=336, Percent_Identity=26.4880952380952, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI284507298, Length=210, Percent_Identity=30.952380952381, Blast_Score=82, Evalue=8e-16,
Organism=Escherichia coli, GI1786349, Length=426, Percent_Identity=65.2582159624413, Blast_Score=582, Evalue=1e-167,
Organism=Escherichia coli, GI1789016, Length=354, Percent_Identity=31.638418079096, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1788044, Length=319, Percent_Identity=33.2288401253918, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1789759, Length=310, Percent_Identity=31.2903225806452, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI145693181, Length=314, Percent_Identity=31.5286624203822, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI1787560, Length=317, Percent_Identity=31.8611987381703, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1786991, Length=382, Percent_Identity=26.1780104712042, Blast_Score=95, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI25144271, Length=298, Percent_Identity=29.1946308724832, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71992977, Length=311, Percent_Identity=27.9742765273312, Blast_Score=95, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17541228, Length=342, Percent_Identity=26.9005847953216, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25144274, Length=143, Percent_Identity=34.2657342657343, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323470, Length=300, Percent_Identity=29.6666666666667, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324432, Length=313, Percent_Identity=25.5591054313099, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21357415, Length=327, Percent_Identity=26.2996941896024, Blast_Score=92, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 45123; Mature: 44992

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRSETLFANAQKHIPGGVNSPVRAFKSVGGTPLFFKHAEGAYVTDEDDKRYVDYVGSWG
CCCCCCCCCCCHHCCCCCCCHHHHHHHHCCCCEEEEEECCCCEECCCCCCHHHHHHCCCC
PMILGHSHPDVLDAVRNQLQHGLSYGAPTAMETEMADLVCSLVPSLEMVRMVSSGTEATM
CEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHCCHHHHH
SAIRLARGFTGRDSIIKFEGCYHGHSDSLLVKAGSGALTQGVPSSAGVPAAFAKHTLTLP
HHHHHHHCCCCCCCEEEEECEECCCCCCEEEEECCCCCCCCCCCCCCCCHHHHCCEEECC
FNDIDAVEKMLAEVGQDVACIIVEPVAGNMNCVPPAPGFLEGLRSLCDKHGVVLIFDEVM
CCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEHHHH
TGFRVALGGAQAYYGVTPDLTTFGKIIGGGMPVGCFGGKRLIMERIAPLGPVYQAGTLSG
HHHHHHHCCCCEEECCCCCHHHHHHHHCCCCCEECCCCHHHHHHHHCCCCCCCCCCCCCC
NPLAMAAGLTTLRLISRPGFHAELTDYTTRLLDGLQQRADAAGIPFVTTQAGGMFGLYFS
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEC
GADDIVTFEDVMASDAALFGRFFHLMLEGGVYLAPSAFEAGFTSIAHGEAELKLTLDAAE
CCCCCEEHHHHHHHHHHHHHHHHHHHHHCCEEECCCHHHHCCHHHCCCCEEEEEEEHHHH
RAFAKLK
HHHHHCC
>Mature Secondary Structure 
SRSETLFANAQKHIPGGVNSPVRAFKSVGGTPLFFKHAEGAYVTDEDDKRYVDYVGSWG
CCCCCCCCCCHHCCCCCCCHHHHHHHHCCCCEEEEEECCCCEECCCCCCHHHHHHCCCC
PMILGHSHPDVLDAVRNQLQHGLSYGAPTAMETEMADLVCSLVPSLEMVRMVSSGTEATM
CEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHCCHHHHH
SAIRLARGFTGRDSIIKFEGCYHGHSDSLLVKAGSGALTQGVPSSAGVPAAFAKHTLTLP
HHHHHHHCCCCCCCEEEEECEECCCCCCEEEEECCCCCCCCCCCCCCCCHHHHCCEEECC
FNDIDAVEKMLAEVGQDVACIIVEPVAGNMNCVPPAPGFLEGLRSLCDKHGVVLIFDEVM
CCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEHHHH
TGFRVALGGAQAYYGVTPDLTTFGKIIGGGMPVGCFGGKRLIMERIAPLGPVYQAGTLSG
HHHHHHHCCCCEEECCCCCHHHHHHHHCCCCCEECCCCHHHHHHHHCCCCCCCCCCCCCC
NPLAMAAGLTTLRLISRPGFHAELTDYTTRLLDGLQQRADAAGIPFVTTQAGGMFGLYFS
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEC
GADDIVTFEDVMASDAALFGRFFHLMLEGGVYLAPSAFEAGFTSIAHGEAELKLTLDAAE
CCCCCEEHHHHHHHHHHHHHHHHHHHHHCCEEECCCHHHHCCHHHCCCCEEEEEEEHHHH
RAFAKLK
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA