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The map label for this gene is thiE

Identifier: 77461167

GI number: 77461167

Start: 5574227

End: 5574850

Strand: Direct

Name: thiE

Synonym: Pfl01_4946

Alternate gene names: 77461167

Gene position: 5574227-5574850 (Clockwise)

Preceding gene: 77461166

Following gene: 77461168

Centisome position: 86.58

GC content: 64.42

Gene sequence:

>624_bases
ATGAAACTACGTGGCCTGTATGCCATCACCGACAGCCAGTTGCTGGCCGGCAAGTTTCTGTCCTACGTGGAGGCGGCGCT
GGAAGGCGGCGTCACCCTGCTGCAATACCGTGACAAGAGCAGCGACGAAGCCCGCCGTCTGCGTGAGGCCGAAGCCCTGC
GCGATCTGTGCGAACGCTACAAGACGCAACTGATCATCAACGATGACGCCGAACTGGCCGCACGCCTGAACGTCGGCGTG
CACCTGGGCCAGACCGACGGCCCGCTGTCGCCGACCCGCGCCCTGCTCGGCTACAAGGCGATCATCGGTTCGACCTGCCA
CGCGCAGCTTGAACTGGCCGAACAGGCCGCCAGGGAAGGCGCGAGTTACGTCGCCTTCGGTCGTTTCTTCAACTCCAACA
CCAAACCCGGTGCACCGAGTTGCAGCCTCGATCTGCTCGACGAGGCCAAACGCACCCTGCACCTGCCGATCTGCGCGATC
GGCGGCATCACCCTCGACAACGCCGCGCCGCTGGTGGCCCACGGTGTCGATCTGCTGGCCGTGGTCCATGGCCTGTTCGG
CGCCAACAGCACCGCCGAAGTGACCCGCCGCGCCCGCGCCTTCAATGAATTACTAAAAGTCTGA

Upstream 100 bases:

>100_bases
CACCCTGCGCGATGCCGAACAGCTGGGCAAAGGCCAGTTCGTGCCGCGTCGCCTGCCGCTGGATTTCTGTTCCTGATGTT
TCGAGCCTGAGGTCTGCGCG

Downstream 100 bases:

>100_bases
GAGCCCGATCATGTCTCGTTCCGAAACCCTGTTTGCCAACGCCCAGAAACACATTCCCGGTGGCGTGAACTCGCCGGTAC
GCGCGTTCAAGAGCGTTGGC

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MKLRGLYAITDSQLLAGKFLSYVEAALEGGVTLLQYRDKSSDEARRLREAEALRDLCERYKTQLIINDDAELAARLNVGV
HLGQTDGPLSPTRALLGYKAIIGSTCHAQLELAEQAAREGASYVAFGRFFNSNTKPGAPSCSLDLLDEAKRTLHLPICAI
GGITLDNAAPLVAHGVDLLAVVHGLFGANSTAEVTRRARAFNELLKV

Sequences:

>Translated_207_residues
MKLRGLYAITDSQLLAGKFLSYVEAALEGGVTLLQYRDKSSDEARRLREAEALRDLCERYKTQLIINDDAELAARLNVGV
HLGQTDGPLSPTRALLGYKAIIGSTCHAQLELAEQAAREGASYVAFGRFFNSNTKPGAPSCSLDLLDEAKRTLHLPICAI
GGITLDNAAPLVAHGVDLLAVVHGLFGANSTAEVTRRARAFNELLKV
>Mature_207_residues
MKLRGLYAITDSQLLAGKFLSYVEAALEGGVTLLQYRDKSSDEARRLREAEALRDLCERYKTQLIINDDAELAARLNVGV
HLGQTDGPLSPTRALLGYKAIIGSTCHAQLELAEQAAREGASYVAFGRFFNSNTKPGAPSCSLDLLDEAKRTLHLPICAI
GGITLDNAAPLVAHGVDLLAVVHGLFGANSTAEVTRRARAFNELLKV

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=185, Percent_Identity=34.0540540540541, Blast_Score=77, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6325042, Length=218, Percent_Identity=30.7339449541284, Blast_Score=90, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_PSEPF (Q3K6C1)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_350674.1
- ProteinModelPortal:   Q3K6C1
- STRING:   Q3K6C1
- GeneID:   3714029
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_4946
- eggNOG:   COG0352
- HOGENOM:   HBG754477
- OMA:   CERYKTR
- ProtClustDB:   PRK00043
- BioCyc:   PFLU205922:PFL_4946-MONOMER
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 22291; Mature: 22291

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRGLYAITDSQLLAGKFLSYVEAALEGGVTLLQYRDKSSDEARRLREAEALRDLCERY
CCCCEEEEECCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
KTQLIINDDAELAARLNVGVHLGQTDGPLSPTRALLGYKAIIGSTCHAQLELAEQAAREG
HHEEEECCCHHHHHHHHCCEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCC
ASYVAFGRFFNSNTKPGAPSCSLDLLDEAKRTLHLPICAIGGITLDNAAPLVAHGVDLLA
CCEEEHHHHHCCCCCCCCCCCCHHHHHHHHHHEECHHHHCCCEEECCCCCHHHHHHHHHH
VVHGLFGANSTAEVTRRARAFNELLKV
HHHHHHCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLRGLYAITDSQLLAGKFLSYVEAALEGGVTLLQYRDKSSDEARRLREAEALRDLCERY
CCCCEEEEECCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
KTQLIINDDAELAARLNVGVHLGQTDGPLSPTRALLGYKAIIGSTCHAQLELAEQAAREG
HHEEEECCCHHHHHHHHCCEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCC
ASYVAFGRFFNSNTKPGAPSCSLDLLDEAKRTLHLPICAIGGITLDNAAPLVAHGVDLLA
CCEEEHHHHHCCCCCCCCCCCCHHHHHHHHHHEECHHHHCCCEEECCCCCHHHHHHHHHH
VVHGLFGANSTAEVTRRARAFNELLKV
HHHHHHCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA