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The map label for this gene is rlmG

Identifier: 77461111

GI number: 77461111

Start: 5514075

End: 5515199

Strand: Direct

Name: rlmG

Synonym: Pfl01_4890

Alternate gene names: 77461111

Gene position: 5514075-5515199 (Clockwise)

Preceding gene: 77461110

Following gene: 77461115

Centisome position: 85.64

GC content: 62.93

Gene sequence:

>1125_bases
ATGCCTTTGCTCGATACGCCCTTCGCCCAGCTTGATCTGATCCGCCAGCCCGAACAGCAGAACGAACCGCTGCAAGCCTT
CGACGCAGCCGACGAATACCTGCTCAATCATCTGGCCGAACAGAATCTGCCGATCGAGACACGGGTTCTGGTGCTCAACG
ACAGTTTCGGCGCGCTGGCCATCAGCCTGTCAGGCAACGTGCAGGTCAGCACCAGCGGCGATTCGTTCCTGGCGTTTCAA
GGCCTTGAAAAAAACCTGCTGCGCAATGGTCAGGCATTCGATGCGCTGCGCGGCATTCCCGCCAGCGAGCCGTTGGTCGG
GCCATTTGACCGGGTGCTGATCCGCGTTCCGAAAACCCTGGCACTGCTGGAAGAACAACTGATTCGCCTGCAAGGCCAAT
TGGCGCCCGGCGCGCAAGTGGTCGCCGCAGCCATGGTCAAACATCTGCCCCGCGCCGCCGGTGATCTGCTGGAACGCTAT
ATCGGCCCGGTTCAGGCTTCGCTGGCGGTGAAGAAGGCACGCTTGCTGATCGCCACGCCCGAGGCGAAGGCACCCGCTGT
CTCGCCCTACCCGACCCGTTATCGCCTCGACGAGCCGGCCATCGAACTGCTCAACCATGCCAACGTGTTCTGCCGTGAAG
GGCTGGACATCGGCACCCGTGCCTTCCTTCCGCACCTGCCGAAAAACCTCGGCGCGGCAGGGGTCGCCGACCTAGGCTGC
GGTAACGGCGTACTGGCCATCGCCAGCGCCCTGCAAAACCCAGATGCGCATTACACGCTGGTGGACGAATCGTTCATGGC
CGTGCAGTCGGCGGCCGAAAACTGGCGCGCGGCGCTGGGTGAACGTGAAGCGATCGTGCGCGCAGGCGACGGCCTTGCCG
GACAGGAAGCGCAATCGCTGGATGTGGTGCTGTGCAACCCGCCGTTTCACCAGCAACAGGTGGTCGGCGACTTCCTTGCC
TGGCGCATGTTCCAGCAGGCCCGCGAAGCACTGGTCGTCGGTGGTGCGCTGTACATCGTCGGCAACCGTCACCTGGGTTA
TCACAGCAAACTGGCGCGACTGTTCCGGGGTGTCGAGCAGGTCGCGGCCACGCCGAAATTCGTGATCCTCAAGGCACGCA
AATAA

Upstream 100 bases:

>100_bases
GTGATGTCGTGAGCGTTCACGTCGCAGTGCACTCGGTTGGTTCCGCCGCACTAAACCCTGGGCCGCGGGACACCCGTCGA
TTACCCAGAGTCCCCGCCCC

Downstream 100 bases:

>100_bases
TCCGGGCAAAAAAAACCCTCCGGTCGGAGGGTTAAAAATCCGTGCCGCAAGGCAGCGGGATGGGAAGTCTTAGTGCGTGG
TCAGGCCTGCTGCATTCATG

Product: 16S rRNA m(2)G 1207 methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G1835 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmG

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MPLLDTPFAQLDLIRQPEQQNEPLQAFDAADEYLLNHLAEQNLPIETRVLVLNDSFGALAISLSGNVQVSTSGDSFLAFQ
GLEKNLLRNGQAFDALRGIPASEPLVGPFDRVLIRVPKTLALLEEQLIRLQGQLAPGAQVVAAAMVKHLPRAAGDLLERY
IGPVQASLAVKKARLLIATPEAKAPAVSPYPTRYRLDEPAIELLNHANVFCREGLDIGTRAFLPHLPKNLGAAGVADLGC
GNGVLAIASALQNPDAHYTLVDESFMAVQSAAENWRAALGEREAIVRAGDGLAGQEAQSLDVVLCNPPFHQQQVVGDFLA
WRMFQQAREALVVGGALYIVGNRHLGYHSKLARLFRGVEQVAATPKFVILKARK

Sequences:

>Translated_374_residues
MPLLDTPFAQLDLIRQPEQQNEPLQAFDAADEYLLNHLAEQNLPIETRVLVLNDSFGALAISLSGNVQVSTSGDSFLAFQ
GLEKNLLRNGQAFDALRGIPASEPLVGPFDRVLIRVPKTLALLEEQLIRLQGQLAPGAQVVAAAMVKHLPRAAGDLLERY
IGPVQASLAVKKARLLIATPEAKAPAVSPYPTRYRLDEPAIELLNHANVFCREGLDIGTRAFLPHLPKNLGAAGVADLGC
GNGVLAIASALQNPDAHYTLVDESFMAVQSAAENWRAALGEREAIVRAGDGLAGQEAQSLDVVLCNPPFHQQQVVGDFLA
WRMFQQAREALVVGGALYIVGNRHLGYHSKLARLFRGVEQVAATPKFVILKARK
>Mature_373_residues
PLLDTPFAQLDLIRQPEQQNEPLQAFDAADEYLLNHLAEQNLPIETRVLVLNDSFGALAISLSGNVQVSTSGDSFLAFQG
LEKNLLRNGQAFDALRGIPASEPLVGPFDRVLIRVPKTLALLEEQLIRLQGQLAPGAQVVAAAMVKHLPRAAGDLLERYI
GPVQASLAVKKARLLIATPEAKAPAVSPYPTRYRLDEPAIELLNHANVFCREGLDIGTRAFLPHLPKNLGAAGVADLGCG
NGVLAIASALQNPDAHYTLVDESFMAVQSAAENWRAALGEREAIVRAGDGLAGQEAQSLDVVLCNPPFHQQQVVGDFLAW
RMFQQAREALVVGGALYIVGNRHLGYHSKLARLFRGVEQVAATPKFVILKARK

Specific function: Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA

COG id: COG2813

COG function: function code J; 16S RNA G1207 methylase RsmC

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmG family

Homologues:

Organism=Escherichia coli, GI87082206, Length=379, Percent_Identity=40.8970976253298, Blast_Score=257, Evalue=9e-70,
Organism=Escherichia coli, GI1790830, Length=364, Percent_Identity=27.1978021978022, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMG_PSEPF (Q3K6H7)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_350618.1
- ProteinModelPortal:   Q3K6H7
- STRING:   Q3K6H7
- GeneID:   3714247
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_4890
- eggNOG:   COG2813
- HOGENOM:   HBG321931
- OMA:   NRHLGYH
- ProtClustDB:   CLSK868782
- BioCyc:   PFLU205922:PFL_4890-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01859
- InterPro:   IPR002052
- InterPro:   IPR017237
- InterPro:   IPR007848
- PIRSF:   PIRSF037565

Pfam domain/function: PF05175 MTS

EC number: =2.1.1.174

Molecular weight: Translated: 40426; Mature: 40295

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLLDTPFAQLDLIRQPEQQNEPLQAFDAADEYLLNHLAEQNLPIETRVLVLNDSFGALA
CCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEE
ISLSGNVQVSTSGDSFLAFQGLEKNLLRNGQAFDALRGIPASEPLVGPFDRVLIRVPKTL
EEECCCEEEEECCCCEEEECCHHHHHHCCCCHHHHHHCCCCCCCCCCHHHHHHHHCCHHH
ALLEEQLIRLQGQLAPGAQVVAAAMVKHLPRAAGDLLERYIGPVQASLAVKKARLLIATP
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCEEEEECC
EAKAPAVSPYPTRYRLDEPAIELLNHANVFCREGLDIGTRAFLPHLPKNLGAAGVADLGC
CCCCCCCCCCCCCEECCHHHHHHHHHCCHHHHCCCCCCHHHHHHHCCHHCCCCCCCCCCC
GNGVLAIASALQNPDAHYTLVDESFMAVQSAAENWRAALGEREAIVRAGDGLAGQEAQSL
CCCHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHCCHHHEEECCCCCCCCCCCCC
DVVLCNPPFHQQQVVGDFLAWRMFQQAREALVVGGALYIVGNRHLGYHSKLARLFRGVEQ
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHH
VAATPKFVILKARK
HHCCCCEEEEEECC
>Mature Secondary Structure 
PLLDTPFAQLDLIRQPEQQNEPLQAFDAADEYLLNHLAEQNLPIETRVLVLNDSFGALA
CCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEE
ISLSGNVQVSTSGDSFLAFQGLEKNLLRNGQAFDALRGIPASEPLVGPFDRVLIRVPKTL
EEECCCEEEEECCCCEEEECCHHHHHHCCCCHHHHHHCCCCCCCCCCHHHHHHHHCCHHH
ALLEEQLIRLQGQLAPGAQVVAAAMVKHLPRAAGDLLERYIGPVQASLAVKKARLLIATP
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCEEEEECC
EAKAPAVSPYPTRYRLDEPAIELLNHANVFCREGLDIGTRAFLPHLPKNLGAAGVADLGC
CCCCCCCCCCCCCEECCHHHHHHHHHCCHHHHCCCCCCHHHHHHHCCHHCCCCCCCCCCC
GNGVLAIASALQNPDAHYTLVDESFMAVQSAAENWRAALGEREAIVRAGDGLAGQEAQSL
CCCHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHCCHHHEEECCCCCCCCCCCCC
DVVLCNPPFHQQQVVGDFLAWRMFQQAREALVVGGALYIVGNRHLGYHSKLARLFRGVEQ
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHH
VAATPKFVILKARK
HHCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA