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The map label for this gene is mscL [H]

Identifier: 77461108

GI number: 77461108

Start: 5511613

End: 5512077

Strand: Direct

Name: mscL [H]

Synonym: Pfl01_4887

Alternate gene names: 77461108

Gene position: 5511613-5512077 (Clockwise)

Preceding gene: 77461105

Following gene: 77461110

Centisome position: 85.61

GC content: 57.2

Gene sequence:

>465_bases
ATGCTGATTTACACTGCGTACACCAAACTCATCTGTAACAAGGAAATAACTATGGGCGTGCTCAGCGAGTTCAAGGCCTT
CGCGGTCAAAGGCAATGTGGTCGACATGGCCGTCGGTATCATCATCGGCGCGGCCTTCGGCAAAATCGTTTCGTCGTTTG
TCGGCGATGTGATCATGCCGCCAATCGGCCTGCTGATCGGCGGAGTGGATTTCAGTGACCTGGCCATCACGCTGAAAGCC
GCCGAGGGCAGCGCCCCTGCGGTGGTGCTGGCGTACGGTAAATTCATCCAGAGCGTTCTTGACTTCGTGATCGTCGCCTT
CGCGATTTTCATGGGCGTCAAAGCCATCAACCGCTTGAAACGCGAAGAAGCCGTAGCGCCTACCTTGCCGCCCGTGCCGA
CCAAGGAAGAAGAACTGCTGGGCGAGATCCGCGATCTGCTCAAGGCCCAGAACAACCGGCCCTGA

Upstream 100 bases:

>100_bases
CGTATTTCGCGCGCGATTAGTGGCACTTTGCAAACTAATTGCGAAATGGTGTGTCGATAAAAACGGGCTTTGTAAGAAGG
TGTTACGCGCAGGACGCGTT

Downstream 100 bases:

>100_bases
AGACTGAAACGGCGCCTGAGGGCGCCGTTTTCTTACCAGTAGTTTTCCACCGCGACCTGCCCCGGTCGCCGGGTCAGACT
CAAGGCCATGCCACGTTGCT

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MLIYTAYTKLICNKEITMGVLSEFKAFAVKGNVVDMAVGIIIGAAFGKIVSSFVGDVIMPPIGLLIGGVDFSDLAITLKA
AEGSAPAVVLAYGKFIQSVLDFVIVAFAIFMGVKAINRLKREEAVAPTLPPVPTKEEELLGEIRDLLKAQNNRP

Sequences:

>Translated_154_residues
MLIYTAYTKLICNKEITMGVLSEFKAFAVKGNVVDMAVGIIIGAAFGKIVSSFVGDVIMPPIGLLIGGVDFSDLAITLKA
AEGSAPAVVLAYGKFIQSVLDFVIVAFAIFMGVKAINRLKREEAVAPTLPPVPTKEEELLGEIRDLLKAQNNRP
>Mature_154_residues
MLIYTAYTKLICNKEITMGVLSEFKAFAVKGNVVDMAVGIIIGAAFGKIVSSFVGDVIMPPIGLLIGGVDFSDLAITLKA
AEGSAPAVVLAYGKFIQSVLDFVIVAFAIFMGVKAINRLKREEAVAPTLPPVPTKEEELLGEIRDLLKAQNNRP

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell [H]

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family [H]

Homologues:

Organism=Escherichia coli, GI1789686, Length=136, Percent_Identity=65.4411764705882, Blast_Score=184, Evalue=3e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019823
- InterPro:   IPR001185 [H]

Pfam domain/function: PF01741 MscL [H]

EC number: NA

Molecular weight: Translated: 16426; Mature: 16426

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIYTAYTKLICNKEITMGVLSEFKAFAVKGNVVDMAVGIIIGAAFGKIVSSFVGDVIMP
CEEHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PIGLLIGGVDFSDLAITLKAAEGSAPAVVLAYGKFIQSVLDFVIVAFAIFMGVKAINRLK
CHHHHHCCCCHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REEAVAPTLPPVPTKEEELLGEIRDLLKAQNNRP
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLIYTAYTKLICNKEITMGVLSEFKAFAVKGNVVDMAVGIIIGAAFGKIVSSFVGDVIMP
CEEHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PIGLLIGGVDFSDLAITLKAAEGSAPAVVLAYGKFIQSVLDFVIVAFAIFMGVKAINRLK
CHHHHHCCCCHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REEAVAPTLPPVPTKEEELLGEIRDLLKAQNNRP
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA