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The map label for this gene is dadA [C]

Identifier: 77461062

GI number: 77461062

Start: 5460703

End: 5461803

Strand: Reverse

Name: dadA [C]

Synonym: Pfl01_4841

Alternate gene names: 77461062

Gene position: 5461803-5460703 (Counterclockwise)

Preceding gene: 77461063

Following gene: 77461057

Centisome position: 84.83

GC content: 64.49

Gene sequence:

>1101_bases
ATGACCAGGCAACAGCAAGTGGTGATTGTCGGTGGCGGGGTGATTGGCCTGCTCACTGCGTACAACCTCGCCTCCGAGGT
CGGCAGCGTGGTGCTGCTGGATCGTTCGAACGTCGGCCAGGAATCGTCCTGGGCCGGCGGCGGTATCGTGTCTCCGCTGT
ACCCGTGGCGCTACAGCCCGGCCGTCACCGCGCTGGCGCACTGGTCGCAGGATTTTTATCCACAGCTGGGCGAGCGCCTT
TTTGCCGATACCGGGGTCGACCCCGAAGTGCAGACCACCGGCCTGTATTGGCTGGACCTGGATGACGAAGCCGAAGCGCT
GGCCTGGGCCGCGCGGGAAAACCGTCCGCTGCGGGCTGTGGATATCTCGGCGGCGCATGATGCGGTGCCGGTGCTCGGAG
GCGGTTTTTCCCGGGCGATCTACATGGCCGATGTGGCCAACGTGCGCAATCCGCGGCTGGTGAAGTCTTTGAAAGCGGCG
TTGCAGGCACTGCCGAATGTGACGATTCACGAGCAGTGTGAAGTCAGCGGGTTTGTCCGTGAGGGCGAGCGGGTGGTGGG
CGTGCAGACCTCCACGGGTTTGATCACCGGTGATCAGGTCGTTCTCACCGCAGGGGCGTGGAGCGGCGAACTGCTCAAGA
ACCTGGACCTGTCGCTGCCGGTGGAACCGGTCAAGGGTCAGATGATTCTGTACAAGTGCGCAGCGGATTTCCTGCCGAGC
ATGGTGCTGGCCAAGGGGCGTTATGCGATTCCGCGCCGTGACGGGCACATTCTGATCGGCAGTACGCTGGAACACGAAGG
CTTCGACAAGACCCCGACGGACAACGCACTGGAAAGCCTCAAGGCCTCGGCAGTCGAGTTGCTGCCGGCGCTGGCCGATG
CCGAAGTGGTGGGGCACTGGGCCGGGCTGCGACCGGGTTCGCCCGAGGGCATTCCCTACATTGGCCGGGTTCCGGGGGTT
GACGGTCTATGGCTGAACTGCGGGCATTACCGCAACGGGCTGGTGCTGGCGCCGGCGTCCTGTCAGTTGTTTGCCGATGT
GATGCTGGGGCGCGCGCCGATCATTGATCCGGCGCCGTATGCACCGGCCGGACGGATCTGA

Upstream 100 bases:

>100_bases
ACGCGACCACCGGGCTGACTACCAAGGATTGCTGGGGCCGCTGAGCCGCTTTATTTCGGGCGCCTTTTGCGCCCACTGTC
TTTTTACGGTTGGATCAAAC

Downstream 100 bases:

>100_bases
AGCCATATCAACAAAACGCAGCCGACAAAGGCTGCGTTCCCCTGAATGTTCAATCCAGCCCCAGTTTCTTGAGCCTGTAG
CGCATCGACCGAAACGAAAG

Product: FAD dependent oxidoreductase

Products: FADH2; ammonia; Pyruvate [C]

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MTRQQQVVIVGGGVIGLLTAYNLASEVGSVVLLDRSNVGQESSWAGGGIVSPLYPWRYSPAVTALAHWSQDFYPQLGERL
FADTGVDPEVQTTGLYWLDLDDEAEALAWAARENRPLRAVDISAAHDAVPVLGGGFSRAIYMADVANVRNPRLVKSLKAA
LQALPNVTIHEQCEVSGFVREGERVVGVQTSTGLITGDQVVLTAGAWSGELLKNLDLSLPVEPVKGQMILYKCAADFLPS
MVLAKGRYAIPRRDGHILIGSTLEHEGFDKTPTDNALESLKASAVELLPALADAEVVGHWAGLRPGSPEGIPYIGRVPGV
DGLWLNCGHYRNGLVLAPASCQLFADVMLGRAPIIDPAPYAPAGRI

Sequences:

>Translated_366_residues
MTRQQQVVIVGGGVIGLLTAYNLASEVGSVVLLDRSNVGQESSWAGGGIVSPLYPWRYSPAVTALAHWSQDFYPQLGERL
FADTGVDPEVQTTGLYWLDLDDEAEALAWAARENRPLRAVDISAAHDAVPVLGGGFSRAIYMADVANVRNPRLVKSLKAA
LQALPNVTIHEQCEVSGFVREGERVVGVQTSTGLITGDQVVLTAGAWSGELLKNLDLSLPVEPVKGQMILYKCAADFLPS
MVLAKGRYAIPRRDGHILIGSTLEHEGFDKTPTDNALESLKASAVELLPALADAEVVGHWAGLRPGSPEGIPYIGRVPGV
DGLWLNCGHYRNGLVLAPASCQLFADVMLGRAPIIDPAPYAPAGRI
>Mature_365_residues
TRQQQVVIVGGGVIGLLTAYNLASEVGSVVLLDRSNVGQESSWAGGGIVSPLYPWRYSPAVTALAHWSQDFYPQLGERLF
ADTGVDPEVQTTGLYWLDLDDEAEALAWAARENRPLRAVDISAAHDAVPVLGGGFSRAIYMADVANVRNPRLVKSLKAAL
QALPNVTIHEQCEVSGFVREGERVVGVQTSTGLITGDQVVLTAGAWSGELLKNLDLSLPVEPVKGQMILYKCAADFLPSM
VLAKGRYAIPRRDGHILIGSTLEHEGFDKTPTDNALESLKASAVELLPALADAEVVGHWAGLRPGSPEGIPYIGRVPGVD
GLWLNCGHYRNGLVLAPASCQLFADVMLGRAPIIDPAPYAPAGRI

Specific function: Oxidative Deamination Of D-Amino Acids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=189, Percent_Identity=33.3333333333333, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076
- InterPro:   IPR012727 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.4.99.1 [C]

Molecular weight: Translated: 39046; Mature: 38915

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRQQQVVIVGGGVIGLLTAYNLASEVGSVVLLDRSNVGQESSWAGGGIVSPLYPWRYSP
CCCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCH
AVTALAHWSQDFYPQLGERLFADTGVDPEVQTTGLYWLDLDDEAEALAWAARENRPLRAV
HHHHHHHHCHHHHHHHHHHHHHCCCCCCCEEECEEEEEECCCCHHHHHHHHHCCCCEEEE
DISAAHDAVPVLGGGFSRAIYMADVANVRNPRLVKSLKAALQALPNVTIHEQCEVSGFVR
ECCCCCCCCEEECCCHHHEEEEEHHHCCCCHHHHHHHHHHHHHCCCCEEECHHHHHHHHH
EGERVVGVQTSTGLITGDQVVLTAGAWSGELLKNLDLSLPVEPVKGQMILYKCAADFLPS
CCCEEEEEECCCCEEECCEEEEEECCCCHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHH
MVLAKGRYAIPRRDGHILIGSTLEHEGFDKTPTDNALESLKASAVELLPALADAEVVGHW
HHHHCCCEECCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGLRPGSPEGIPYIGRVPGVDGLWLNCGHYRNGLVLAPASCQLFADVMLGRAPIIDPAPY
CCCCCCCCCCCCEECCCCCCCCEEEECCCCCCCEEECCCHHHHHHHHHHCCCCCCCCCCC
APAGRI
CCCCCC
>Mature Secondary Structure 
TRQQQVVIVGGGVIGLLTAYNLASEVGSVVLLDRSNVGQESSWAGGGIVSPLYPWRYSP
CCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCH
AVTALAHWSQDFYPQLGERLFADTGVDPEVQTTGLYWLDLDDEAEALAWAARENRPLRAV
HHHHHHHHCHHHHHHHHHHHHHCCCCCCCEEECEEEEEECCCCHHHHHHHHHCCCCEEEE
DISAAHDAVPVLGGGFSRAIYMADVANVRNPRLVKSLKAALQALPNVTIHEQCEVSGFVR
ECCCCCCCCEEECCCHHHEEEEEHHHCCCCHHHHHHHHHHHHHCCCCEEECHHHHHHHHH
EGERVVGVQTSTGLITGDQVVLTAGAWSGELLKNLDLSLPVEPVKGQMILYKCAADFLPS
CCCEEEEEECCCCEEECCEEEEEECCCCHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHH
MVLAKGRYAIPRRDGHILIGSTLEHEGFDKTPTDNALESLKASAVELLPALADAEVVGHW
HHHHCCCEECCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGLRPGSPEGIPYIGRVPGVDGLWLNCGHYRNGLVLAPASCQLFADVMLGRAPIIDPAPY
CCCCCCCCCCCCEECCCCCCCCEEEECCCCCCCEEECCCHHHHHHHHHHCCCCCCCCCCC
APAGRI
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {D-proline}} 0.13 {K3Fe(CN)6}} 11 {D-methionine}} 6 {D-phenylalanine}} 12 {D-alpha-aminobutyrate}} 14 {D-glutamine}} 0.63 {dichloroindophenol}} 0.01 {dichloroindophenol}} 0.15 {cytochrome} 19 {D-serine}} 29 {D-alanine}} 6.4

Substrates: D-Alanine; FAD; H2O [C]

Specific reaction: D-Alanine + FAD + H2O --> FADH2 + ammonia + Pyruvate [C]

General reaction: Oxidative deamination; Redox reaction [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8682785; 10984043; 8097014 [H]