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The map label for this gene is pilR [H]

Identifier: 77461061

GI number: 77461061

Start: 5459307

End: 5460653

Strand: Direct

Name: pilR [H]

Synonym: Pfl01_4840

Alternate gene names: 77461061

Gene position: 5459307-5460653 (Clockwise)

Preceding gene: 77461060

Following gene: 77461069

Centisome position: 84.79

GC content: 61.84

Gene sequence:

>1347_bases
TTGAACACGAGCCCACGGCAAAAAATCCTCATCGTCGACGACGAGCCGGATATCCGCGAACTCCTAGAAATCACCCTGGG
ACGGATGAAACTCGACACCTTCAGCGCACGCAATCTCGAAGAAGCCCAGGCACTGCTGCACCGCGAGACCTTCGATCTGT
GCCTGACCGACATGCGCCTGCCCGACGGCACCGGCCTGGAGTTGGTGCAGCACATTCAGCAACGTTATCCACAACTGCCG
GTGGCGATGATCACCGCCTACGGCAGCCTGGAAACGGCAATCAACGCCCTGAAGGCCGGGGCATTCGACTTCCTGACCAA
ACCGGTGGACCTGACACGCCTGCGCGAGCTGGTCGGCTCGGCATTGCGCATGCCGGCGCCGGGCAATGTCTGCACTTCGA
TCGACCGGCGGTTGCTCGGCGATTCACCGCCGATGCGCAACCTGCGCAAGCAGATCGACAAACTGGCCCGCAGCCAGGCA
CCGGTCTACATCAGCGGCGAGTCCGGCAGCGGCAAGGAGCTGGTGGCCCGACTGATTCATGAACAGGGCCCGCGCGCCAG
CCAGCCGTTCGTGCCGGTGAACTGCGGGGCGATTCCGTCTGAGTTGATGGAAAGCGAGTTCTTCGGCCACCGCAAAGGCA
GTTTCAGCGGCGCGGTGGAGGATAAACCGGGGCTGTTTCAGGCGGCTCATGGCGGCACACTGTTTCTCGATGAAGTGGCG
GACCTGCCACTGCCGATGCAGGTCAAGCTGCTGCGGGCGATCCAGGAAAAAGCCGTGCGCAGCGTCGGCGGCCAACAGGA
AACCGTGGTCGATGTGCGCATACTCTGCGCGACGCACAAGGATCTCGATGCTGAAGTGGCTGCCGAGCGATTCCGTCAGG
ATCTGTATTACCGGCTGAACGTGATCGAACTGCGTGTACCGTCCCTGCGCGAACGTCGCGAGGACATCGAAGCACTGGCC
GGTCACATGCTCAAGCGCCTGGCCAATGGCACCGGGCAGCCGGCGGCACGCCTTCATCCCCATGCCCTCGAGGCGCTGAA
AAACTACCGTTTTCCGGGCAACGTGAGGGAGCTGGAGAACGTGCTTGAACGGGCGCACACCCTGTGTGAAAACCGCACGA
TCGAGGCCGAGGATTTACGCCTGAGTGAAGGTAATGGCGCCGCCGAGGCCGGGATTGCCGATCTGACGCAGATCGACAAC
CTGGAAGACTATCTGGAAAGCGTCGAACGCAAACTGATTCTGCAGGCGCTGGAAGAAACCCGCTGGAATCGCACGGCGGC
GGCGCAGCGGTTGAGTCTTTCGTTTCGGTCGATGCGCTACAGGCTCAAGAAACTGGGGCTGGATTGA

Upstream 100 bases:

>100_bases
CCCGTGAGCTGTGCGAAAGCAATCAAGCGCGCCTAGACTTCAAATCACGCCAAGGCGGCGGCTGCTTTCGCATCACCTTT
GCTCACGGACGGAAACAAAG

Downstream 100 bases:

>100_bases
ACATTCAGGGGAACGCAGCCTTTGTCGGCTGCGTTTTGTTGATATGGCTTCAGATCCGTCCGGCCGGTGCATACGGCGCC
GGATCAATGATCGGCGCGCG

Product: two-component response regulator PilR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MNTSPRQKILIVDDEPDIRELLEITLGRMKLDTFSARNLEEAQALLHRETFDLCLTDMRLPDGTGLELVQHIQQRYPQLP
VAMITAYGSLETAINALKAGAFDFLTKPVDLTRLRELVGSALRMPAPGNVCTSIDRRLLGDSPPMRNLRKQIDKLARSQA
PVYISGESGSGKELVARLIHEQGPRASQPFVPVNCGAIPSELMESEFFGHRKGSFSGAVEDKPGLFQAAHGGTLFLDEVA
DLPLPMQVKLLRAIQEKAVRSVGGQQETVVDVRILCATHKDLDAEVAAERFRQDLYYRLNVIELRVPSLRERREDIEALA
GHMLKRLANGTGQPAARLHPHALEALKNYRFPGNVRELENVLERAHTLCENRTIEAEDLRLSEGNGAAEAGIADLTQIDN
LEDYLESVERKLILQALEETRWNRTAAAQRLSLSFRSMRYRLKKLGLD

Sequences:

>Translated_448_residues
MNTSPRQKILIVDDEPDIRELLEITLGRMKLDTFSARNLEEAQALLHRETFDLCLTDMRLPDGTGLELVQHIQQRYPQLP
VAMITAYGSLETAINALKAGAFDFLTKPVDLTRLRELVGSALRMPAPGNVCTSIDRRLLGDSPPMRNLRKQIDKLARSQA
PVYISGESGSGKELVARLIHEQGPRASQPFVPVNCGAIPSELMESEFFGHRKGSFSGAVEDKPGLFQAAHGGTLFLDEVA
DLPLPMQVKLLRAIQEKAVRSVGGQQETVVDVRILCATHKDLDAEVAAERFRQDLYYRLNVIELRVPSLRERREDIEALA
GHMLKRLANGTGQPAARLHPHALEALKNYRFPGNVRELENVLERAHTLCENRTIEAEDLRLSEGNGAAEAGIADLTQIDN
LEDYLESVERKLILQALEETRWNRTAAAQRLSLSFRSMRYRLKKLGLD
>Mature_448_residues
MNTSPRQKILIVDDEPDIRELLEITLGRMKLDTFSARNLEEAQALLHRETFDLCLTDMRLPDGTGLELVQHIQQRYPQLP
VAMITAYGSLETAINALKAGAFDFLTKPVDLTRLRELVGSALRMPAPGNVCTSIDRRLLGDSPPMRNLRKQIDKLARSQA
PVYISGESGSGKELVARLIHEQGPRASQPFVPVNCGAIPSELMESEFFGHRKGSFSGAVEDKPGLFQAAHGGTLFLDEVA
DLPLPMQVKLLRAIQEKAVRSVGGQQETVVDVRILCATHKDLDAEVAAERFRQDLYYRLNVIELRVPSLRERREDIEALA
GHMLKRLANGTGQPAARLHPHALEALKNYRFPGNVRELENVLERAHTLCENRTIEAEDLRLSEGNGAAEAGIADLTQIDN
LEDYLESVERKLILQALEETRWNRTAAAQRLSLSFRSMRYRLKKLGLD

Specific function: Member of the two-component regulatory system pilS/pilR that regulates the expression of type 4 fimbriae. When activated by pilS it acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=452, Percent_Identity=40.4867256637168, Blast_Score=336, Evalue=2e-93,
Organism=Escherichia coli, GI1790437, Length=440, Percent_Identity=42.5, Blast_Score=333, Evalue=2e-92,
Organism=Escherichia coli, GI1788905, Length=370, Percent_Identity=42.972972972973, Blast_Score=301, Evalue=6e-83,
Organism=Escherichia coli, GI1790299, Length=467, Percent_Identity=38.5438972162741, Blast_Score=300, Evalue=2e-82,
Organism=Escherichia coli, GI87082117, Length=320, Percent_Identity=40.9375, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI87082152, Length=317, Percent_Identity=41.6403785488959, Blast_Score=228, Evalue=7e-61,
Organism=Escherichia coli, GI1789233, Length=323, Percent_Identity=39.6284829721362, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI1789087, Length=345, Percent_Identity=39.1304347826087, Blast_Score=218, Evalue=7e-58,
Organism=Escherichia coli, GI1786524, Length=241, Percent_Identity=46.4730290456432, Blast_Score=209, Evalue=4e-55,
Organism=Escherichia coli, GI1787583, Length=311, Percent_Identity=36.0128617363344, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI87081872, Length=318, Percent_Identity=37.4213836477987, Blast_Score=189, Evalue=5e-49,
Organism=Escherichia coli, GI1789828, Length=243, Percent_Identity=35.8024691358025, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI87081858, Length=324, Percent_Identity=30.2469135802469, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1787487, Length=140, Percent_Identity=37.1428571428571, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786599, Length=139, Percent_Identity=30.2158273381295, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1789809, Length=116, Percent_Identity=34.4827586206897, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50105; Mature: 50105

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTSPRQKILIVDDEPDIRELLEITLGRMKLDTFSARNLEEAQALLHRETFDLCLTDMRL
CCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHCCC
PDGTGLELVQHIQQRYPQLPVAMITAYGSLETAINALKAGAFDFLTKPVDLTRLRELVGS
CCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCHHHHHHHHHH
ALRMPAPGNVCTSIDRRLLGDSPPMRNLRKQIDKLARSQAPVYISGESGSGKELVARLIH
HHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHH
EQGPRASQPFVPVNCGAIPSELMESEFFGHRKGSFSGAVEDKPGLFQAAHGGTLFLDEVA
HCCCCCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCEEEHHHHH
DLPLPMQVKLLRAIQEKAVRSVGGQQETVVDVRILCATHKDLDAEVAAERFRQDLYYRLN
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
VIELRVPSLRERREDIEALAGHMLKRLANGTGQPAARLHPHALEALKNYRFPGNVRELEN
HEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHCCCCCCHHHHHH
VLERAHTLCENRTIEAEDLRLSEGNGAAEAGIADLTQIDNLEDYLESVERKLILQALEET
HHHHHHHHHHCCCCCHHHCEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
RWNRTAAAQRLSLSFRSMRYRLKKLGLD
HCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNTSPRQKILIVDDEPDIRELLEITLGRMKLDTFSARNLEEAQALLHRETFDLCLTDMRL
CCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHCCC
PDGTGLELVQHIQQRYPQLPVAMITAYGSLETAINALKAGAFDFLTKPVDLTRLRELVGS
CCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCHHHHHHHHHH
ALRMPAPGNVCTSIDRRLLGDSPPMRNLRKQIDKLARSQAPVYISGESGSGKELVARLIH
HHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHH
EQGPRASQPFVPVNCGAIPSELMESEFFGHRKGSFSGAVEDKPGLFQAAHGGTLFLDEVA
HCCCCCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCEEEHHHHH
DLPLPMQVKLLRAIQEKAVRSVGGQQETVVDVRILCATHKDLDAEVAAERFRQDLYYRLN
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
VIELRVPSLRERREDIEALAGHMLKRLANGTGQPAARLHPHALEALKNYRFPGNVRELEN
HEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHCCCCCCHHHHHH
VLERAHTLCENRTIEAEDLRLSEGNGAAEAGIADLTQIDNLEDYLESVERKLILQALEET
HHHHHHHHHHCCCCCHHHCEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
RWNRTAAAQRLSLSFRSMRYRLKKLGLD
HCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1317379; 8097014; 7911557; 10984043 [H]