Click here to switch to the map view.

The map label for this gene is pilS [H]

Identifier: 77461060

GI number: 77461060

Start: 5457721

End: 5459310

Strand: Direct

Name: pilS [H]

Synonym: Pfl01_4839

Alternate gene names: 77461060

Gene position: 5457721-5459310 (Clockwise)

Preceding gene: 77461059

Following gene: 77461061

Centisome position: 84.77

GC content: 61.45

Gene sequence:

>1590_bases
GTGATCGCTGAGGCCGACAACGCCGACAGCAAACAGGCTCAGCGACTGCTGCGCCTCTATCATCTGTACCGTTTGAGTGT
CGGCATCACCCTGGTGTTGCTGATCTCCAGCAACATGGACAACCGCCTGCTGACCTCGGCCAACGACGAGTGGCTGCGCA
ATGGCAGCTGGTTGTACCTGGTGCTGAACATCCTGCTGGTGGTGTTTCTCGAGAACATTCGCCGCCCCGCCCAGTTGTTC
GGCCTGGCACTGGTCGACATCCTGCTGCTATGCGGCGTGTTCTATGCGGCCGGCGGCGTGGCCAGCGCGATCGGCAACCT
GCTGATCGTCTCGGTGGCCATCAGCAACACCTTGCTGCGACGACGTATCGGCTTGCTGATCGCCGCCATCGCCACCCTCG
GCATCGTCGGGCTCAGCTTTCTGCTGAGTTTCAGCCATCCCCTGAGCGCCAACGATTATCTGCAGGCCGGCACCCTCGGC
GCCCTCTGCTTCGCGGCGTCATTGCTGGTGCAAGGTTTGATGCGCCGTCTCGAAGTCAGTGAGAACCTGGCCGAACAGCG
CGCCAGCGAAGTCGTGGGGCTGGAAGCCCTCAACGCATTGATCCTGCAACGCATGCGCACCGGCATTCTGGTGCTCGACG
AACAACGCCGGGTGCAACTGGCCAACCACAGTGCCAGGGTCCTGCTGGGCCAAACGCATCTTGAAGGTGACTTGATCGAC
GAGCACTCGAGCGCATTGGTCGAACGCCTGCAACTGTGGCTGAACAACCCGACCCTGCGGCCGCAAAGCCTGAAAATTGC
CGGCAACGGCCTGGAGCTGCAGCCGAGTTTCATCGCCCTGGAACAGAACCCGAACCGCCAGACCCTGGTGTTTCTTGAAG
ACCTCGCCCAGATAGCCCAGCAGGCCCAACAATTGAAACTGGCTGCGCTGGGCCGGCTGACCGCCGGTATCGCCCATGAA
ATCCGCAATCCGCTGGGGGCCATCAGTCATGCAGCGCAGCTGTTGCAGGAGTCCGAGGAACTCGACAGCGCGGATCGGCG
TCTGACGCAGATCATTCAAGACCACTCCCAGCGCATGAACCGAGTCATCGAAAACGTCCTGCAACTGTCCCGCCGCCAGC
AAAGCGCACCGCAACGGCTCGACCTCAAGCCGTGGCTGGAGCAGTTCGTCAACGAGAGCCGCGAACGCTCCGGCGAGCGC
CAGCACATTCACCTGCACATCGGCCTGGGGGATTTCCGCACGTTGATGGATCCCGGCCAGCTCACGCAGATTCTCGACAA
TCTGTTACGCAACGGCTGGCGCCACAGCGCCCTTTTGCACGACCCGGCCGAGGTCTGGATGACGCTGTTCATCGACCCCG
AGAGCACGCTGGCCGTGCTCGAAGTGCAGGACAACGGCCCCGGCGTGCCGCTGGATCAACAGGCTCACCTGTTCGAACCG
TTTTTTACTACCAGTAGCCAGGGTACCGGCCTTGGGCTTTATCTGTCCCGTGAGCTGTGCGAAAGCAATCAAGCGCGCCT
AGACTTCAAATCACGCCAAGGCGGCGGCTGCTTTCGCATCACCTTTGCTCACGGACGGAAACAAAGTTGA

Upstream 100 bases:

>100_bases
TGCGCCCACTGCGGCGTGCATCTGCCCCGCGACCGCGCGCTGAACCTTCAACAACAGTGGTATTGCAGCCAGGCTCATCT
TGAGCAAGGCCCCGGCTCCA

Downstream 100 bases:

>100_bases
ACACGAGCCCACGGCAAAAAATCCTCATCGTCGACGACGAGCCGGATATCCGCGAACTCCTAGAAATCACCCTGGGACGG
ATGAAACTCGACACCTTCAG

Product: sensor protein PilS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MIAEADNADSKQAQRLLRLYHLYRLSVGITLVLLISSNMDNRLLTSANDEWLRNGSWLYLVLNILLVVFLENIRRPAQLF
GLALVDILLLCGVFYAAGGVASAIGNLLIVSVAISNTLLRRRIGLLIAAIATLGIVGLSFLLSFSHPLSANDYLQAGTLG
ALCFAASLLVQGLMRRLEVSENLAEQRASEVVGLEALNALILQRMRTGILVLDEQRRVQLANHSARVLLGQTHLEGDLID
EHSSALVERLQLWLNNPTLRPQSLKIAGNGLELQPSFIALEQNPNRQTLVFLEDLAQIAQQAQQLKLAALGRLTAGIAHE
IRNPLGAISHAAQLLQESEELDSADRRLTQIIQDHSQRMNRVIENVLQLSRRQQSAPQRLDLKPWLEQFVNESRERSGER
QHIHLHIGLGDFRTLMDPGQLTQILDNLLRNGWRHSALLHDPAEVWMTLFIDPESTLAVLEVQDNGPGVPLDQQAHLFEP
FFTTSSQGTGLGLYLSRELCESNQARLDFKSRQGGGCFRITFAHGRKQS

Sequences:

>Translated_529_residues
MIAEADNADSKQAQRLLRLYHLYRLSVGITLVLLISSNMDNRLLTSANDEWLRNGSWLYLVLNILLVVFLENIRRPAQLF
GLALVDILLLCGVFYAAGGVASAIGNLLIVSVAISNTLLRRRIGLLIAAIATLGIVGLSFLLSFSHPLSANDYLQAGTLG
ALCFAASLLVQGLMRRLEVSENLAEQRASEVVGLEALNALILQRMRTGILVLDEQRRVQLANHSARVLLGQTHLEGDLID
EHSSALVERLQLWLNNPTLRPQSLKIAGNGLELQPSFIALEQNPNRQTLVFLEDLAQIAQQAQQLKLAALGRLTAGIAHE
IRNPLGAISHAAQLLQESEELDSADRRLTQIIQDHSQRMNRVIENVLQLSRRQQSAPQRLDLKPWLEQFVNESRERSGER
QHIHLHIGLGDFRTLMDPGQLTQILDNLLRNGWRHSALLHDPAEVWMTLFIDPESTLAVLEVQDNGPGVPLDQQAHLFEP
FFTTSSQGTGLGLYLSRELCESNQARLDFKSRQGGGCFRITFAHGRKQS
>Mature_529_residues
MIAEADNADSKQAQRLLRLYHLYRLSVGITLVLLISSNMDNRLLTSANDEWLRNGSWLYLVLNILLVVFLENIRRPAQLF
GLALVDILLLCGVFYAAGGVASAIGNLLIVSVAISNTLLRRRIGLLIAAIATLGIVGLSFLLSFSHPLSANDYLQAGTLG
ALCFAASLLVQGLMRRLEVSENLAEQRASEVVGLEALNALILQRMRTGILVLDEQRRVQLANHSARVLLGQTHLEGDLID
EHSSALVERLQLWLNNPTLRPQSLKIAGNGLELQPSFIALEQNPNRQTLVFLEDLAQIAQQAQQLKLAALGRLTAGIAHE
IRNPLGAISHAAQLLQESEELDSADRRLTQIIQDHSQRMNRVIENVLQLSRRQQSAPQRLDLKPWLEQFVNESRERSGER
QHIHLHIGLGDFRTLMDPGQLTQILDNLLRNGWRHSALLHDPAEVWMTLFIDPESTLAVLEVQDNGPGVPLDQQAHLFEP
FFTTSSQGTGLGLYLSRELCESNQARLDFKSRQGGGCFRITFAHGRKQS

Specific function: Member of the two-component regulatory system pilS/pilR involved in the regulation of the expression of type 4 fimbriae. PilS may function as a membrane-associated protein kinase that phosphorylates pilR in response to environmental signals [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=345, Percent_Identity=28.4057971014493, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1790436, Length=215, Percent_Identity=31.6279069767442, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1790300, Length=239, Percent_Identity=29.2887029288703, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1789808, Length=209, Percent_Identity=26.3157894736842, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87082128, Length=222, Percent_Identity=24.7747747747748, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1790861, Length=237, Percent_Identity=25.3164556962025, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1786912, Length=248, Percent_Identity=27.0161290322581, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI145693157, Length=249, Percent_Identity=26.9076305220884, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI87081816, Length=232, Percent_Identity=29.3103448275862, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790346, Length=198, Percent_Identity=29.7979797979798, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786600, Length=250, Percent_Identity=23.6, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 58831; Mature: 58831

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAEADNADSKQAQRLLRLYHLYRLSVGITLVLLISSNMDNRLLTSANDEWLRNGSWLYL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCEECCCCHHHHHCCCHHHH
VLNILLVVFLENIRRPAQLFGLALVDILLLCGVFYAAGGVASAIGNLLIVSVAISNTLLR
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
RRIGLLIAAIATLGIVGLSFLLSFSHPLSANDYLQAGTLGALCFAASLLVQGLMRRLEVS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHH
ENLAEQRASEVVGLEALNALILQRMRTGILVLDEQRRVQLANHSARVLLGQTHLEGDLID
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHCCCCEEEEECCCCCCCHHH
EHSSALVERLQLWLNNPTLRPQSLKIAGNGLELQPSFIALEQNPNRQTLVFLEDLAQIAQ
HHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEECCCEEEEECCCCCCEEHHHHHHHHHHH
QAQQLKLAALGRLTAGIAHEIRNPLGAISHAAQLLQESEELDSADRRLTQIIQDHSQRMN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVIENVLQLSRRQQSAPQRLDLKPWLEQFVNESRERSGERQHIHLHIGLGDFRTLMDPGQ
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECHHHHHHCCCHH
LTQILDNLLRNGWRHSALLHDPAEVWMTLFIDPESTLAVLEVQDNGPGVPLDQQAHLFEP
HHHHHHHHHHCCCCCCCCCCCHHHEEEEEEECCCCCEEEEEEECCCCCCCCCCCCHHHCC
FFTTSSQGTGLGLYLSRELCESNQARLDFKSRQGGGCFRITFAHGRKQS
HHCCCCCCCCEEHHEEHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCC
>Mature Secondary Structure
MIAEADNADSKQAQRLLRLYHLYRLSVGITLVLLISSNMDNRLLTSANDEWLRNGSWLYL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCEECCCCHHHHHCCCHHHH
VLNILLVVFLENIRRPAQLFGLALVDILLLCGVFYAAGGVASAIGNLLIVSVAISNTLLR
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
RRIGLLIAAIATLGIVGLSFLLSFSHPLSANDYLQAGTLGALCFAASLLVQGLMRRLEVS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHH
ENLAEQRASEVVGLEALNALILQRMRTGILVLDEQRRVQLANHSARVLLGQTHLEGDLID
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHCCCCEEEEECCCCCCCHHH
EHSSALVERLQLWLNNPTLRPQSLKIAGNGLELQPSFIALEQNPNRQTLVFLEDLAQIAQ
HHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEECCCEEEEECCCCCCEEHHHHHHHHHHH
QAQQLKLAALGRLTAGIAHEIRNPLGAISHAAQLLQESEELDSADRRLTQIIQDHSQRMN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVIENVLQLSRRQQSAPQRLDLKPWLEQFVNESRERSGERQHIHLHIGLGDFRTLMDPGQ
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECHHHHHHCCCHH
LTQILDNLLRNGWRHSALLHDPAEVWMTLFIDPESTLAVLEVQDNGPGVPLDQQAHLFEP
HHHHHHHHHHCCCCCCCCCCCHHHEEEEEEECCCCCEEEEEEECCCCCCCCCCCCHHHCC
FFTTSSQGTGLGLYLSRELCESNQARLDFKSRQGGGCFRITFAHGRKQS
HHCCCCCCCCEEHHEEHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8097014; 10984043 [H]