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The map label for this gene is acsA2 [H]

Identifier: 77461035

GI number: 77461035

Start: 5431759

End: 5433696

Strand: Direct

Name: acsA2 [H]

Synonym: Pfl01_4814

Alternate gene names: 77461035

Gene position: 5431759-5433696 (Clockwise)

Preceding gene: 77461034

Following gene: 77461036

Centisome position: 84.36

GC content: 62.02

Gene sequence:

>1938_bases
ATGTTCGATATCAGCACGTTCCCCAAAGCCGATGCCGTCCGCCGGGCTGCACAGTTGAGTCAGGACGACTACCAGCGCCT
GTACCGCGAATCCATTGAACACCCCAGCACCTTCTGGGCCGAACAGGCCACCCGCTTCCTCGACTGGAGCACGCCGTGGC
AGACCGTCCAGCGCTATGACCTGAAGACCGGCGACGCCGCCTGGTTTGCCGGTGGCAAGCTGAACGTCAGCTACAACTGC
ATCGACCGCCATCTGGAAACGCGCGGCGATCAGACTGCCATCCTCTGGGAAGGCGACGACCCTGCCGAATCGACGCAAAT
CACCTACAGCAAACTCCATCACCACGTCTGCCGCCTGGCCAACGTGCTGAAAAGCCGTGGCGTAAAAAAAGGCGACCGGG
TGTGCATCTACATGCCGATGATCCCCGAAGCGGCCTACGCCATGCTCGCCTGCGCACGGATCGGCGCGATTCACTCGGTA
GTGTTTGGCGGTTTCTCCCCGGACTCATTGCGCGACCGCATTCTCGACGCCGACTGCCGCACCGTGATCACCGCCGACGA
GGGCGTGCGCGGTGGCAAATTCGTGCCGCTGAAACAGAACGTCGACAAAGCCCTGCAGAGTTGTCCGAATGTCAGCACCG
TCGTGGTGGTCGAGCGCACCCAGAACAAAGTCGACTGGGTCGAAGGCCGCGACCTCTGGTATCACCAGGCCGTGCGCGAC
GTCAGCGACGAATGCCCGCCGGAACCGATGGACGCAGAAGACCCGCTGTTCATCCTCTACACCTCCGGCAGCACCGGCAA
ACCCAAAGGCGTGCTGCACACCACCGGCGGTTACCTGCTGCAAGCGGCGATGACCTTCAAGTACGTCCTCGACTACCGCG
ATGGCGAAGTGTTCTGGTGCACCGCCGACGTCGGCTGGGTTACCGGCCACAGCTATATCGTCTACGGCCCGCTGGCCAAC
GGCGCCACCACACTGATCTTCGAAGGCGTGCCGAGCTACCCGAGCACTTCGCGGTTCTGGCAGGTGATCGACAAACACAA
GGTCAACATCTTCTACACCGCCCCCACCGCCCTGCGCGCATTGATGCGTGAAGGCGCCGGGCCCTTGCAGGAAACGTCGC
GACAAAGCCTCAAATTGCTCGGCAGTGTCGGTGAGCCGATCAACCCGGAAGCGTGGGAATGGTATTTCAACGTCGTAGGC
GAACAGCGCTGCCCGATTGTCGATACGTGGTGGCAGACCGAAACCGGCGGCATCATGCTCAGCCCGTTGGTCAGCGCTCA
GCAGATCAAACCAGGCTGCGCCACCCGGCCGATGTTCGGCGTGCAACCGGTGCTGCTCGATGAGCACGGCAAGGAAATCA
AGGGCGCCGGCAGTGGCGTACTGGCGATCAAGTCGAGCTGGCCGGCGCAGATCCGCAGCGTTTACGGCGATCCGCAGCGC
ATGGTCGACACCTACTTCAAGCCCTACCCCGGTTACTACTTCACCGGCGACGGTGCCCGTCGTGACGAGGACGGCGATTA
CTGGATCACCGGGCGCATCGATGACGTGATCAATGTCTCCGGCCATCGCATCGGCACCGCCGAGGTGGAAAGTGCGTTGG
TGCTGCACGACAGCATTGCAGAGGCCGCCGTGGTCGGTTATCCCCACGACGTCAAAGGTCAGGGGATTTATGCGTTCGTC
ACACCGATGAATGGCACCGAACCCAACGATGAGCTGAAGAAAGAACTGCTGGCCCACGTCAGCAAGGAAATCGGCAGCTT
CGCCAAACCGGACCTGATCCAGTGGGCCCCGGCCCTGCCGAAGACCCGCTCGGGCAAGATCATGCGGCGGATCCTGCGCA
AGATCGCCTGCAACGAGCTCGACAGCCTTGGCGACACCTCGACGCTGGCGGATCCGAGCGTGGTGCAGGGTTTGATCGAT
AAACGCTTGAACCAGTAA

Upstream 100 bases:

>100_bases
CACCCCCTCGCCATGAGGGGGTGTTTGTTCACAGGTATTGAACCCTCTCCGCTCTCGGCGCATCTTTATCCTTGTCGCAC
AACAAGAATAAGGAACCGTC

Downstream 100 bases:

>100_bases
GGCTGCAAAAGCATCGCGGGCAGACTCGCTCCCTCAGGGACTTATGATCGACTGTGGGAGCGAGCCTGCTCACGATGGGA
CCCCCTCGATTGCCTGCCAA

Product: acetyl-CoA synthetase

Products: NA

Alternate protein names: Acetate--CoA ligase 2; Acyl-activating enzyme 2 [H]

Number of amino acids: Translated: 645; Mature: 645

Protein sequence:

>645_residues
MFDISTFPKADAVRRAAQLSQDDYQRLYRESIEHPSTFWAEQATRFLDWSTPWQTVQRYDLKTGDAAWFAGGKLNVSYNC
IDRHLETRGDQTAILWEGDDPAESTQITYSKLHHHVCRLANVLKSRGVKKGDRVCIYMPMIPEAAYAMLACARIGAIHSV
VFGGFSPDSLRDRILDADCRTVITADEGVRGGKFVPLKQNVDKALQSCPNVSTVVVVERTQNKVDWVEGRDLWYHQAVRD
VSDECPPEPMDAEDPLFILYTSGSTGKPKGVLHTTGGYLLQAAMTFKYVLDYRDGEVFWCTADVGWVTGHSYIVYGPLAN
GATTLIFEGVPSYPSTSRFWQVIDKHKVNIFYTAPTALRALMREGAGPLQETSRQSLKLLGSVGEPINPEAWEWYFNVVG
EQRCPIVDTWWQTETGGIMLSPLVSAQQIKPGCATRPMFGVQPVLLDEHGKEIKGAGSGVLAIKSSWPAQIRSVYGDPQR
MVDTYFKPYPGYYFTGDGARRDEDGDYWITGRIDDVINVSGHRIGTAEVESALVLHDSIAEAAVVGYPHDVKGQGIYAFV
TPMNGTEPNDELKKELLAHVSKEIGSFAKPDLIQWAPALPKTRSGKIMRRILRKIACNELDSLGDTSTLADPSVVQGLID
KRLNQ

Sequences:

>Translated_645_residues
MFDISTFPKADAVRRAAQLSQDDYQRLYRESIEHPSTFWAEQATRFLDWSTPWQTVQRYDLKTGDAAWFAGGKLNVSYNC
IDRHLETRGDQTAILWEGDDPAESTQITYSKLHHHVCRLANVLKSRGVKKGDRVCIYMPMIPEAAYAMLACARIGAIHSV
VFGGFSPDSLRDRILDADCRTVITADEGVRGGKFVPLKQNVDKALQSCPNVSTVVVVERTQNKVDWVEGRDLWYHQAVRD
VSDECPPEPMDAEDPLFILYTSGSTGKPKGVLHTTGGYLLQAAMTFKYVLDYRDGEVFWCTADVGWVTGHSYIVYGPLAN
GATTLIFEGVPSYPSTSRFWQVIDKHKVNIFYTAPTALRALMREGAGPLQETSRQSLKLLGSVGEPINPEAWEWYFNVVG
EQRCPIVDTWWQTETGGIMLSPLVSAQQIKPGCATRPMFGVQPVLLDEHGKEIKGAGSGVLAIKSSWPAQIRSVYGDPQR
MVDTYFKPYPGYYFTGDGARRDEDGDYWITGRIDDVINVSGHRIGTAEVESALVLHDSIAEAAVVGYPHDVKGQGIYAFV
TPMNGTEPNDELKKELLAHVSKEIGSFAKPDLIQWAPALPKTRSGKIMRRILRKIACNELDSLGDTSTLADPSVVQGLID
KRLNQ
>Mature_645_residues
MFDISTFPKADAVRRAAQLSQDDYQRLYRESIEHPSTFWAEQATRFLDWSTPWQTVQRYDLKTGDAAWFAGGKLNVSYNC
IDRHLETRGDQTAILWEGDDPAESTQITYSKLHHHVCRLANVLKSRGVKKGDRVCIYMPMIPEAAYAMLACARIGAIHSV
VFGGFSPDSLRDRILDADCRTVITADEGVRGGKFVPLKQNVDKALQSCPNVSTVVVVERTQNKVDWVEGRDLWYHQAVRD
VSDECPPEPMDAEDPLFILYTSGSTGKPKGVLHTTGGYLLQAAMTFKYVLDYRDGEVFWCTADVGWVTGHSYIVYGPLAN
GATTLIFEGVPSYPSTSRFWQVIDKHKVNIFYTAPTALRALMREGAGPLQETSRQSLKLLGSVGEPINPEAWEWYFNVVG
EQRCPIVDTWWQTETGGIMLSPLVSAQQIKPGCATRPMFGVQPVLLDEHGKEIKGAGSGVLAIKSSWPAQIRSVYGDPQR
MVDTYFKPYPGYYFTGDGARRDEDGDYWITGRIDDVINVSGHRIGTAEVESALVLHDSIAEAAVVGYPHDVKGQGIYAFV
TPMNGTEPNDELKKELLAHVSKEIGSFAKPDLIQWAPALPKTRSGKIMRRILRKIACNELDSLGDTSTLADPSVVQGLID
KRLNQ

Specific function: Enables The Cell To Use Acetate During Aerobic Growth To Generate Energy Via The Tca Cycle, And Biosynthetic Compounds Via The Glyoxylate Shunt. Acetylates Chey, The Response Regulator Involved In Flagellar Movement And Chemotaxis. [C]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI8923896, Length=668, Percent_Identity=53.7425149700599, Blast_Score=708, Evalue=0.0,
Organism=Homo sapiens, GI253970489, Length=681, Percent_Identity=53.0102790014684, Blast_Score=707, Evalue=0.0,
Organism=Homo sapiens, GI28416953, Length=619, Percent_Identity=51.8578352180937, Blast_Score=673, Evalue=0.0,
Organism=Homo sapiens, GI13375727, Length=650, Percent_Identity=37.0769230769231, Blast_Score=394, Evalue=1e-109,
Organism=Homo sapiens, GI42544132, Length=566, Percent_Identity=28.4452296819788, Blast_Score=199, Evalue=5e-51,
Organism=Homo sapiens, GI38505220, Length=552, Percent_Identity=28.6231884057971, Blast_Score=197, Evalue=3e-50,
Organism=Homo sapiens, GI122937307, Length=551, Percent_Identity=27.7676950998185, Blast_Score=192, Evalue=6e-49,
Organism=Homo sapiens, GI115511026, Length=538, Percent_Identity=28.2527881040892, Blast_Score=192, Evalue=9e-49,
Organism=Homo sapiens, GI31982927, Length=621, Percent_Identity=24.6376811594203, Blast_Score=182, Evalue=8e-46,
Organism=Homo sapiens, GI58082049, Length=554, Percent_Identity=27.4368231046931, Blast_Score=162, Evalue=1e-39,
Organism=Homo sapiens, GI157311624, Length=551, Percent_Identity=26.1343012704174, Blast_Score=152, Evalue=1e-36,
Organism=Homo sapiens, GI157311622, Length=551, Percent_Identity=26.1343012704174, Blast_Score=152, Evalue=1e-36,
Organism=Homo sapiens, GI42544134, Length=384, Percent_Identity=25.5208333333333, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI195947378, Length=440, Percent_Identity=24.0909090909091, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI187761345, Length=413, Percent_Identity=25.6658595641646, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI187761343, Length=413, Percent_Identity=25.6658595641646, Blast_Score=95, Evalue=2e-19,
Organism=Escherichia coli, GI1790505, Length=629, Percent_Identity=61.0492845786963, Blast_Score=821, Evalue=0.0,
Organism=Escherichia coli, GI1786529, Length=626, Percent_Identity=38.4984025559105, Blast_Score=401, Evalue=1e-113,
Organism=Escherichia coli, GI1788107, Length=567, Percent_Identity=23.9858906525573, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI145693145, Length=527, Percent_Identity=24.6679316888046, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71983001, Length=662, Percent_Identity=49.0936555891239, Blast_Score=615, Evalue=1e-176,
Organism=Caenorhabditis elegans, GI71982997, Length=662, Percent_Identity=49.0936555891239, Blast_Score=613, Evalue=1e-176,
Organism=Caenorhabditis elegans, GI17569507, Length=638, Percent_Identity=22.257053291536, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17557194, Length=539, Percent_Identity=23.9332096474954, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17538037, Length=557, Percent_Identity=23.8779174147217, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17559526, Length=558, Percent_Identity=23.2974910394265, Blast_Score=100, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17558820, Length=539, Percent_Identity=22.4489795918367, Blast_Score=90, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17560140, Length=525, Percent_Identity=21.1428571428571, Blast_Score=84, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI32563687, Length=538, Percent_Identity=21.003717472119, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17531443, Length=558, Percent_Identity=22.5806451612903, Blast_Score=79, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI71985884, Length=394, Percent_Identity=23.3502538071066, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323182, Length=637, Percent_Identity=50.392464678179, Blast_Score=654, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6319264, Length=635, Percent_Identity=51.6535433070866, Blast_Score=648, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6319699, Length=367, Percent_Identity=26.7029972752044, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24667955, Length=662, Percent_Identity=52.1148036253776, Blast_Score=689, Evalue=0.0,
Organism=Drosophila melanogaster, GI62472339, Length=562, Percent_Identity=56.2277580071174, Blast_Score=640, Evalue=0.0,
Organism=Drosophila melanogaster, GI24667959, Length=520, Percent_Identity=56.1538461538462, Blast_Score=596, Evalue=1e-170,
Organism=Drosophila melanogaster, GI21355225, Length=642, Percent_Identity=35.6697819314642, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI18859661, Length=530, Percent_Identity=24.7169811320755, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21355181, Length=543, Percent_Identity=23.572744014733, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI28573631, Length=551, Percent_Identity=21.9600725952813, Blast_Score=90, Evalue=5e-18,
Organism=Drosophila melanogaster, GI20130353, Length=551, Percent_Identity=21.9600725952813, Blast_Score=90, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24656500, Length=376, Percent_Identity=23.1382978723404, Blast_Score=75, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011904
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.1 [H]

Molecular weight: Translated: 71738; Mature: 71738

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDISTFPKADAVRRAAQLSQDDYQRLYRESIEHPSTFWAEQATRFLDWSTPWQTVQRYD
CCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHC
LKTGDAAWFAGGKLNVSYNCIDRHLETRGDQTAILWEGDDPAESTQITYSKLHHHVCRLA
CCCCCEEEEECCEEEEEHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHH
NVLKSRGVKKGDRVCIYMPMIPEAAYAMLACARIGAIHSVVFGGFSPDSLRDRILDADCR
HHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCC
TVITADEGVRGGKFVPLKQNVDKALQSCPNVSTVVVVERTQNKVDWVEGRDLWYHQAVRD
EEEECCCCCCCCEEEECHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHH
VSDECPPEPMDAEDPLFILYTSGSTGKPKGVLHTTGGYLLQAAMTFKYVLDYRDGEVFWC
HHHCCCCCCCCCCCCEEEEEECCCCCCCCCEEECCCHHHHHHHHHHHHEEEECCCEEEEE
TADVGWVTGHSYIVYGPLANGATTLIFEGVPSYPSTSRFWQVIDKHKVNIFYTAPTALRA
ECCCCEEECCCEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHCCCEEEEEECHHHHHH
LMREGAGPLQETSRQSLKLLGSVGEPINPEAWEWYFNVVGEQRCPIVDTWWQTETGGIML
HHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCCCCEEE
SPLVSAQQIKPGCATRPMFGVQPVLLDEHGKEIKGAGSGVLAIKSSWPAQIRSVYGDPQR
CCCCCHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCEEEEECCCCHHHHHHHCCHHH
MVDTYFKPYPGYYFTGDGARRDEDGDYWITGRIDDVINVSGHRIGTAEVESALVLHDSIA
HHHHHCCCCCCEEECCCCCCCCCCCCEEEEECCCCEEECCCCEECHHHHHHHHHHHHHHH
EAAVVGYPHDVKGQGIYAFVTPMNGTEPNDELKKELLAHVSKEIGSFAKPDLIQWAPALP
HHHEECCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCC
KTRSGKIMRRILRKIACNELDSLGDTSTLADPSVVQGLIDKRLNQ
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MFDISTFPKADAVRRAAQLSQDDYQRLYRESIEHPSTFWAEQATRFLDWSTPWQTVQRYD
CCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHC
LKTGDAAWFAGGKLNVSYNCIDRHLETRGDQTAILWEGDDPAESTQITYSKLHHHVCRLA
CCCCCEEEEECCEEEEEHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHH
NVLKSRGVKKGDRVCIYMPMIPEAAYAMLACARIGAIHSVVFGGFSPDSLRDRILDADCR
HHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCC
TVITADEGVRGGKFVPLKQNVDKALQSCPNVSTVVVVERTQNKVDWVEGRDLWYHQAVRD
EEEECCCCCCCCEEEECHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHH
VSDECPPEPMDAEDPLFILYTSGSTGKPKGVLHTTGGYLLQAAMTFKYVLDYRDGEVFWC
HHHCCCCCCCCCCCCEEEEEECCCCCCCCCEEECCCHHHHHHHHHHHHEEEECCCEEEEE
TADVGWVTGHSYIVYGPLANGATTLIFEGVPSYPSTSRFWQVIDKHKVNIFYTAPTALRA
ECCCCEEECCCEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHCCCEEEEEECHHHHHH
LMREGAGPLQETSRQSLKLLGSVGEPINPEAWEWYFNVVGEQRCPIVDTWWQTETGGIML
HHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCCCCEEE
SPLVSAQQIKPGCATRPMFGVQPVLLDEHGKEIKGAGSGVLAIKSSWPAQIRSVYGDPQR
CCCCCHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCEEEEECCCCHHHHHHHCCHHH
MVDTYFKPYPGYYFTGDGARRDEDGDYWITGRIDDVINVSGHRIGTAEVESALVLHDSIA
HHHHHCCCCCCEEECCCCCCCCCCCCEEEEECCCCEEECCCCEECHHHHHHHHHHHHHHH
EAAVVGYPHDVKGQGIYAFVTPMNGTEPNDELKKELLAHVSKEIGSFAKPDLIQWAPALP
HHHEECCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCC
KTRSGKIMRRILRKIACNELDSLGDTSTLADPSVVQGLIDKRLNQ
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]