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The map label for this gene is proP [H]

Identifier: 77460988

GI number: 77460988

Start: 5373354

End: 5374706

Strand: Reverse

Name: proP [H]

Synonym: Pfl01_4767

Alternate gene names: 77460988

Gene position: 5374706-5373354 (Counterclockwise)

Preceding gene: 77460994

Following gene: 77460987

Centisome position: 83.48

GC content: 63.64

Gene sequence:

>1353_bases
ATGGGCGCCTTGGTTTTTTGCCTGCCAGAGTTCTTTACCATGACTGCCCATTCCACTGCCCGACCGGCGCCGTTCAGCCG
TTCCGACTACAAGACCCTCGGCCTTGCGGCCCTGGGCGGGGCGCTGGAGATCTACGATTTCATCATTTTCGTGTTCTTCG
CCCTGACCCTCAGCCAGTTGTTCTTCCCGCCGGAAATGCCCGAGTGGCTGCGCCTGCTGCAAAGCTTCGGGATCTTCGTC
ACCGGCTATCTGGCGCGCCCGCTGGGCGGGATCCTGATGGCGCATTTCGCCGACCGGTTGGGGCGCAAGAAAGTCTTCAG
CCTGAGCATCCTGATGATGGCGCTGCCGTGCCTGCTGATCGGGATCATGCCGACCTACGCGCAGATCGGCTATTTCGCAC
CATTGTTGTTGTTGGCGTTGCGCATCCTGCAAGGCGCGGCAGTGGGCGGTGAAGTGCCGAGCGCCTGGGTGTTCGTGGCC
GAGCACGCGCCGGCCGGGCATCGCGGTTATGCCCTGGGCTTTCTGCAGGCCGGGCTGACCTTCGGTTATCTGATCGGCGC
ATTGACCGCGACTTTCCTGGCGCAAGTGTTCACCCCGGCCGAAGTGCTCGATTACGCCTGGCGTTATCCGTTCCTGCTCG
GTGGGGTGTTCGGTGTGATCGGCGTCTGGTTGCGTCGCTGGTTGAGCGAAACTCCTGTTTTCATGGCCATGGAAGCGCAG
CGCGAGGCGCGGGTCGAACTCCCGCTGCGCACCGTGCTGCGCGATCATCGACTGGCCATGTTGCCGGCGATGCTGCTGAC
CTGCGTACTGACCTCGGCGGTAGTGGTGTTCGTGGTCATTACCCCGACCATGATGCAGAAAACCTTCGGCATGACCGCCG
GCCATACTTTTGCGTTGAGCGCGCTGGGCATTGTTTTCCTGAACATCGGCTGCGTGATCGCCGGACGGCTGGTCGATCGC
ATCGGCGCCTGGCGCACGGTCATGCTCTACAGTCTGTTGCTGCCACTGGGGATCGGCCTGCTTTACACCTGCCTGATCAG
CGGCGGGGACTGGATCGGTCTGGCCTACGCCATCGCCGGGCTGTGCTGTGGCGTGGTCGGTGCGGTGCCGTCAGTGATGG
TCAGCCTGTTCCCGGCGCGGATCCGCGTTTCCGGGATCTCCTTCACCTACAACATTGCCTACGCCGCCTGGGCCAGTGTC
ACGCCGTTGCTGCTGATCGGTCTGATGCCATGGAGCCCGTGGATCTGCGTGATGTTCTGCGCGGTGATGGGCGCGGTGGG
CGTCGTCAGTGCAGCGTATTTCGGCTCGCGGATGCCGCGCGCGGAGCCGTGTCCGGTGGCCGGCACCGCCTGA

Upstream 100 bases:

>100_bases
CAGCTGCCAACAGTAAATCAACCGGCGGATTTTTGTGACCGTGTTTTGCTGAATCAGACTATGGTCTAACGGGTTGTCCG
GCGGCCCGTTTGCGTAGACC

Downstream 100 bases:

>100_bases
GTTGTCGAGGCTTAACTATTTTTGTAGGACAAGCCTCGCGCGCTCTTCAGAAAAGATTTCCAAGGCCGATGGCTAGGCTG
CATACCCGCTATCCATCCAG

Product: general substrate transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MGALVFCLPEFFTMTAHSTARPAPFSRSDYKTLGLAALGGALEIYDFIIFVFFALTLSQLFFPPEMPEWLRLLQSFGIFV
TGYLARPLGGILMAHFADRLGRKKVFSLSILMMALPCLLIGIMPTYAQIGYFAPLLLLALRILQGAAVGGEVPSAWVFVA
EHAPAGHRGYALGFLQAGLTFGYLIGALTATFLAQVFTPAEVLDYAWRYPFLLGGVFGVIGVWLRRWLSETPVFMAMEAQ
REARVELPLRTVLRDHRLAMLPAMLLTCVLTSAVVVFVVITPTMMQKTFGMTAGHTFALSALGIVFLNIGCVIAGRLVDR
IGAWRTVMLYSLLLPLGIGLLYTCLISGGDWIGLAYAIAGLCCGVVGAVPSVMVSLFPARIRVSGISFTYNIAYAAWASV
TPLLLIGLMPWSPWICVMFCAVMGAVGVVSAAYFGSRMPRAEPCPVAGTA

Sequences:

>Translated_450_residues
MGALVFCLPEFFTMTAHSTARPAPFSRSDYKTLGLAALGGALEIYDFIIFVFFALTLSQLFFPPEMPEWLRLLQSFGIFV
TGYLARPLGGILMAHFADRLGRKKVFSLSILMMALPCLLIGIMPTYAQIGYFAPLLLLALRILQGAAVGGEVPSAWVFVA
EHAPAGHRGYALGFLQAGLTFGYLIGALTATFLAQVFTPAEVLDYAWRYPFLLGGVFGVIGVWLRRWLSETPVFMAMEAQ
REARVELPLRTVLRDHRLAMLPAMLLTCVLTSAVVVFVVITPTMMQKTFGMTAGHTFALSALGIVFLNIGCVIAGRLVDR
IGAWRTVMLYSLLLPLGIGLLYTCLISGGDWIGLAYAIAGLCCGVVGAVPSVMVSLFPARIRVSGISFTYNIAYAAWASV
TPLLLIGLMPWSPWICVMFCAVMGAVGVVSAAYFGSRMPRAEPCPVAGTA
>Mature_449_residues
GALVFCLPEFFTMTAHSTARPAPFSRSDYKTLGLAALGGALEIYDFIIFVFFALTLSQLFFPPEMPEWLRLLQSFGIFVT
GYLARPLGGILMAHFADRLGRKKVFSLSILMMALPCLLIGIMPTYAQIGYFAPLLLLALRILQGAAVGGEVPSAWVFVAE
HAPAGHRGYALGFLQAGLTFGYLIGALTATFLAQVFTPAEVLDYAWRYPFLLGGVFGVIGVWLRRWLSETPVFMAMEAQR
EARVELPLRTVLRDHRLAMLPAMLLTCVLTSAVVVFVVITPTMMQKTFGMTAGHTFALSALGIVFLNIGCVIAGRLVDRI
GAWRTVMLYSLLLPLGIGLLYTCLISGGDWIGLAYAIAGLCCGVVGAVPSVMVSLFPARIRVSGISFTYNIAYAAWASVT
PLLLIGLMPWSPWICVMFCAVMGAVGVVSAAYFGSRMPRAEPCPVAGTA

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=431, Percent_Identity=30.1624129930394, Blast_Score=165, Evalue=7e-42,
Organism=Escherichia coli, GI1788292, Length=401, Percent_Identity=29.426433915212, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1789941, Length=417, Percent_Identity=30.2158273381295, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1788942, Length=397, Percent_Identity=32.7455919395466, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI87082231, Length=241, Percent_Identity=23.6514522821577, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 48644; Mature: 48513

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGALVFCLPEFFTMTAHSTARPAPFSRSDYKTLGLAALGGALEIYDFIIFVFFALTLSQL
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
FFPPEMPEWLRLLQSFGIFVTGYLARPLGGILMAHFADRLGRKKVFSLSILMMALPCLLI
CCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
GIMPTYAQIGYFAPLLLLALRILQGAAVGGEVPSAWVFVAEHAPAGHRGYALGFLQAGLT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHH
FGYLIGALTATFLAQVFTPAEVLDYAWRYPFLLGGVFGVIGVWLRRWLSETPVFMAMEAQ
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHH
REARVELPLRTVLRDHRLAMLPAMLLTCVLTSAVVVFVVITPTMMQKTFGMTAGHTFALS
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
ALGIVFLNIGCVIAGRLVDRIGAWRTVMLYSLLLPLGIGLLYTCLISGGDWIGLAYAIAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LCCGVVGAVPSVMVSLFPARIRVSGISFTYNIAYAAWASVTPLLLIGLMPWSPWICVMFC
HHHHHHHHHHHHHHHHHHHHHEEEEEEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
AVMGAVGVVSAAYFGSRMPRAEPCPVAGTA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GALVFCLPEFFTMTAHSTARPAPFSRSDYKTLGLAALGGALEIYDFIIFVFFALTLSQL
CCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
FFPPEMPEWLRLLQSFGIFVTGYLARPLGGILMAHFADRLGRKKVFSLSILMMALPCLLI
CCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
GIMPTYAQIGYFAPLLLLALRILQGAAVGGEVPSAWVFVAEHAPAGHRGYALGFLQAGLT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHH
FGYLIGALTATFLAQVFTPAEVLDYAWRYPFLLGGVFGVIGVWLRRWLSETPVFMAMEAQ
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHH
REARVELPLRTVLRDHRLAMLPAMLLTCVLTSAVVVFVVITPTMMQKTFGMTAGHTFALS
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
ALGIVFLNIGCVIAGRLVDRIGAWRTVMLYSLLLPLGIGLLYTCLISGGDWIGLAYAIAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LCCGVVGAVPSVMVSLFPARIRVSGISFTYNIAYAAWASVTPLLLIGLMPWSPWICVMFC
HHHHHHHHHHHHHHHHHHHHHEEEEEEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
AVMGAVGVVSAAYFGSRMPRAEPCPVAGTA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]