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The map label for this gene is phrB [H]

Identifier: 77460957

GI number: 77460957

Start: 5341280

End: 5342746

Strand: Direct

Name: phrB [H]

Synonym: Pfl01_4736

Alternate gene names: 77460957

Gene position: 5341280-5342746 (Clockwise)

Preceding gene: 77460956

Following gene: 77460958

Centisome position: 82.96

GC content: 61.9

Gene sequence:

>1467_bases
ATGATTCAGACAGAAACCGACATGCAATTGATCTGGCTGCGCAGCGACTTGCGCCAACATGACAACACTGCCCTCGCAGC
CGCCGCCGAGCGTGGCCCGAGCGTGGCCGTGTACCTGTTGAGCCCGCAGCAATGGCAGGAACATGACGACGCGCCGTGCA
AGATCGATTTCTGGCTGCGCAATCTGCACAAGCTGAGCAAAAGCCTCGGCGACCTGAACATCCCGCTCCTGATCCGCACC
GCGCCTCACTGGGATCAGGCACCGGCGGTGCTGCTCGAGCTGTGTCGGCAACTGAAGATCGAGGCAGTGCACGTCAACGA
GGAATACGGCATCAACGAAAGCCGTCGCGACGCAGCCGTTGCCAAGGCGCTGCAAGCCGAAGGCATAACGTTCCATAGCT
ACCTCGACCAATTGCTGTTCAAGCCCGGCAGCGTGCTGACCCGCACCGGCACTTACTTTCAGGTGTTCAGTCAGTTCCGC
AAAGTCTGCTACGAGCACCTGCACCGTTCGATGCCTTCGCTGAAACAGGCGCCGGGCAAACAGTCAGCGCTGAACATCGC
CAGCGACCCCGTTCCAGAAGCCATCGAAGGATTCCCGACTCCCGGCGCATCCCTGCGCGCCCTGTGGCCCGCCGGTGAAA
CCGAGGCCCGGCGACGCCTCGACACGTTCGCCGATGCACAGATCGACTATTACAAGAGCGAACGTGACTTCCCGGCCAAA
CCCGGCACCAGCCAGCTCTCGGCTTATCTGGCCGCCGGCGTGATCTCGCCGCGCCAATGCCTGCACGCGGCCCTGCAAAG
CAATCAGGGCGAATTCGAAAGCGGCAAGGTCGGCGCTGTCACGTGGATCAATGAATTACTGTGGCGTGAGTTTTACAAAC
ACATCCTCGTCGGCTATCCGCGGGTTTCACGGCATCGCGCATTCCGTCCGGAAACCGAAGACCTGGCCTGGCGCAATGCC
CCCGAGGACCTGGCCGCCTGGCAAGAGGCCCGCACCGGCTTGCCGATCATCGACGCGGCCATGCGCCAACTGCTGGAAAC
CGGCTGGATGCACAACCGCCTGCGAATGGTGGTGGCGATGTTTCTGACCAAGAACCTGTTGATCGACTGGCGCGAAGGCG
AGCGTTTCTTCATGCGCCACCTGATCGACGGCGACCTGGCCGCGAACAACGGCGGCTGGCAGTGGAGCGCCTCGACCGGC
ACCGACTCGGCGCCGTACTTTCGCATCTTCAACCCGGTCAGTCAGTCGGAAAAGTTCGACAGCGAAGGCCTTTTCATCAA
GCACTGGCTGCCGGAACTGGCCGGCCTGAACAAGAAAGAAATCCACAACCCGGCCAGTGTCGGCGGGCTGTTCGGCGTCG
CGGACTATCCGGCGCCAATCGTCAATCTGAGTGCGTCACGGGAACGGGCGCTGGCAGCGTTCAAAAACCTGCCGTCGCGC
TCGTCTCAAGGAGAGAGCGATGAGTGA

Upstream 100 bases:

>100_bases
ACGCCGAGTTGTCCGCCAGAACTTCCGAAATCGCAGATCTGCTGCTGGCCGAAGATCCTCTTTCGGCGCATCGGGCACTC
GTTCAGCGTGGGTTGATTTA

Downstream 100 bases:

>100_bases
ATTTCTGCGCGGTTTCGCCCGGCGGTTCGCACAGTTGAATAAAGACAACTTGCGAAAACTGGACGAACTGTACAGCGAGG
ACATTCATTTCACCGATCCG

Product: deoxyribodipyrimidine photo-lyase type I

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MIQTETDMQLIWLRSDLRQHDNTALAAAAERGPSVAVYLLSPQQWQEHDDAPCKIDFWLRNLHKLSKSLGDLNIPLLIRT
APHWDQAPAVLLELCRQLKIEAVHVNEEYGINESRRDAAVAKALQAEGITFHSYLDQLLFKPGSVLTRTGTYFQVFSQFR
KVCYEHLHRSMPSLKQAPGKQSALNIASDPVPEAIEGFPTPGASLRALWPAGETEARRRLDTFADAQIDYYKSERDFPAK
PGTSQLSAYLAAGVISPRQCLHAALQSNQGEFESGKVGAVTWINELLWREFYKHILVGYPRVSRHRAFRPETEDLAWRNA
PEDLAAWQEARTGLPIIDAAMRQLLETGWMHNRLRMVVAMFLTKNLLIDWREGERFFMRHLIDGDLAANNGGWQWSASTG
TDSAPYFRIFNPVSQSEKFDSEGLFIKHWLPELAGLNKKEIHNPASVGGLFGVADYPAPIVNLSASRERALAAFKNLPSR
SSQGESDE

Sequences:

>Translated_488_residues
MIQTETDMQLIWLRSDLRQHDNTALAAAAERGPSVAVYLLSPQQWQEHDDAPCKIDFWLRNLHKLSKSLGDLNIPLLIRT
APHWDQAPAVLLELCRQLKIEAVHVNEEYGINESRRDAAVAKALQAEGITFHSYLDQLLFKPGSVLTRTGTYFQVFSQFR
KVCYEHLHRSMPSLKQAPGKQSALNIASDPVPEAIEGFPTPGASLRALWPAGETEARRRLDTFADAQIDYYKSERDFPAK
PGTSQLSAYLAAGVISPRQCLHAALQSNQGEFESGKVGAVTWINELLWREFYKHILVGYPRVSRHRAFRPETEDLAWRNA
PEDLAAWQEARTGLPIIDAAMRQLLETGWMHNRLRMVVAMFLTKNLLIDWREGERFFMRHLIDGDLAANNGGWQWSASTG
TDSAPYFRIFNPVSQSEKFDSEGLFIKHWLPELAGLNKKEIHNPASVGGLFGVADYPAPIVNLSASRERALAAFKNLPSR
SSQGESDE
>Mature_488_residues
MIQTETDMQLIWLRSDLRQHDNTALAAAAERGPSVAVYLLSPQQWQEHDDAPCKIDFWLRNLHKLSKSLGDLNIPLLIRT
APHWDQAPAVLLELCRQLKIEAVHVNEEYGINESRRDAAVAKALQAEGITFHSYLDQLLFKPGSVLTRTGTYFQVFSQFR
KVCYEHLHRSMPSLKQAPGKQSALNIASDPVPEAIEGFPTPGASLRALWPAGETEARRRLDTFADAQIDYYKSERDFPAK
PGTSQLSAYLAAGVISPRQCLHAALQSNQGEFESGKVGAVTWINELLWREFYKHILVGYPRVSRHRAFRPETEDLAWRNA
PEDLAAWQEARTGLPIIDAAMRQLLETGWMHNRLRMVVAMFLTKNLLIDWREGERFFMRHLIDGDLAANNGGWQWSASTG
TDSAPYFRIFNPVSQSEKFDSEGLFIKHWLPELAGLNKKEIHNPASVGGLFGVADYPAPIVNLSASRERALAAFKNLPSR
SSQGESDE

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI4758072, Length=479, Percent_Identity=29.8538622129436, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI188536100, Length=485, Percent_Identity=29.4845360824742, Blast_Score=159, Evalue=7e-39,
Organism=Homo sapiens, GI188536103, Length=431, Percent_Identity=29.2343387470998, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI1786926, Length=470, Percent_Identity=44.2553191489362, Blast_Score=395, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6324962, Length=509, Percent_Identity=32.8094302554028, Blast_Score=267, Evalue=2e-72,
Organism=Drosophila melanogaster, GI17137248, Length=484, Percent_Identity=28.3057851239669, Blast_Score=162, Evalue=6e-40,
Organism=Drosophila melanogaster, GI24585455, Length=484, Percent_Identity=28.3057851239669, Blast_Score=162, Evalue=6e-40,
Organism=Drosophila melanogaster, GI24648152, Length=524, Percent_Identity=26.1450381679389, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 54982; Mature: 54982

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQTETDMQLIWLRSDLRQHDNTALAAAAERGPSVAVYLLSPQQWQEHDDAPCKIDFWLR
CCCCCCCCEEEEEHHHHHHCCCHHHHHHHHCCCCEEEEEECCHHHHCCCCCCCCHHHHHH
NLHKLSKSLGDLNIPLLIRTAPHWDQAPAVLLELCRQLKIEAVHVNEEYGINESRRDAAV
HHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHH
AKALQAEGITFHSYLDQLLFKPGSVLTRTGTYFQVFSQFRKVCYEHLHRSMPSLKQAPGK
HHHHHHCCCCHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCC
QSALNIASDPVPEAIEGFPTPGASLRALWPAGETEARRRLDTFADAQIDYYKSERDFPAK
CHHCCCCCCCCCHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHCHHHHHHCCCCCCCC
PGTSQLSAYLAAGVISPRQCLHAALQSNQGEFESGKVGAVTWINELLWREFYKHILVGYP
CCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
RVSRHRAFRPETEDLAWRNAPEDLAAWQEARTGLPIIDAAMRQLLETGWMHNRLRMVVAM
CHHHCCCCCCCHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FLTKNLLIDWREGERFFMRHLIDGDLAANNGGWQWSASTGTDSAPYFRIFNPVSQSEKFD
HHHHHHEEECCCCHHHHHHHHHCCCCEECCCCEEEECCCCCCCCCEEEEECCHHHCCCCC
SEGLFIKHWLPELAGLNKKEIHNPASVGGLFGVADYPAPIVNLSASRERALAAFKNLPSR
CCCEEHHHHHHHHHCCCHHHCCCCHHHCCCCCCCCCCCCCEECCCHHHHHHHHHHCCCCC
SSQGESDE
CCCCCCCC
>Mature Secondary Structure
MIQTETDMQLIWLRSDLRQHDNTALAAAAERGPSVAVYLLSPQQWQEHDDAPCKIDFWLR
CCCCCCCCEEEEEHHHHHHCCCHHHHHHHHCCCCEEEEEECCHHHHCCCCCCCCHHHHHH
NLHKLSKSLGDLNIPLLIRTAPHWDQAPAVLLELCRQLKIEAVHVNEEYGINESRRDAAV
HHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHH
AKALQAEGITFHSYLDQLLFKPGSVLTRTGTYFQVFSQFRKVCYEHLHRSMPSLKQAPGK
HHHHHHCCCCHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCC
QSALNIASDPVPEAIEGFPTPGASLRALWPAGETEARRRLDTFADAQIDYYKSERDFPAK
CHHCCCCCCCCCHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHCHHHHHHCCCCCCCC
PGTSQLSAYLAAGVISPRQCLHAALQSNQGEFESGKVGAVTWINELLWREFYKHILVGYP
CCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
RVSRHRAFRPETEDLAWRNAPEDLAAWQEARTGLPIIDAAMRQLLETGWMHNRLRMVVAM
CHHHCCCCCCCHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FLTKNLLIDWREGERFFMRHLIDGDLAANNGGWQWSASTGTDSAPYFRIFNPVSQSEKFD
HHHHHHEEECCCCHHHHHHHHHCCCCEECCCCEEEECCCCCCCCCEEEEECCHHHCCCCC
SEGLFIKHWLPELAGLNKKEIHNPASVGGLFGVADYPAPIVNLSASRERALAAFKNLPSR
CCCEEHHHHHHHHHCCCHHHCCCCHHHCCCCCCCCCCCCCEECCCHHHHHHHHHHCCCCC
SSQGESDE
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6325460; 8905232; 9278503; 2200511; 7604260 [H]