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The map label for this gene is mraW

Identifier: 77460902

GI number: 77460902

Start: 5284718

End: 5285665

Strand: Reverse

Name: mraW

Synonym: Pfl01_4681

Alternate gene names: 77460902

Gene position: 5285665-5284718 (Counterclockwise)

Preceding gene: 77460903

Following gene: 77460901

Centisome position: 82.1

GC content: 61.92

Gene sequence:

>948_bases
GTGACTATTGATAGCGGCTTTAACCACATCACCGTACTGCTTGACGAAGCCGTCGAGGCTCTCGCCGTACGCCCTGATGG
CTGCTATCTGGACGGCACGTTCGGACGCGGCGGGCATAGCCGGTTGATCCTGAGCAAGCTCGGGCCGGACGGTCGGCTCA
TCGGATTCGACAAGGATCCTCAAGCGATTGCCACCGGGCAAACGCTAGCGGCCGAAGACGGCCGCTTTGTCGTTGTGCAG
CGCAGCTTTGCCGAGCTCGGTTCGGTCGTTGCCGAACGGGGGCTGACAGGCAAGGTCAGCGGCATTCTGCTGGATCTGGG
CGTGTCTTCGCCGCAGCTCGATGACGCCGAGCGCGGCTTCAGTTTTCTCAACGACGGCCCGCTGGACATGCGCATGGATC
CGTCCCGTGGCATCAGCGCAGCCGAGTTCGTCAACACCGCGCCGGTGGAAGAAATTGCCCGTGTCTTCAAAGAGTACGGC
GAAGAACGTTTCTCCGGTCGCATGGCCCGTGCCGTTGTCGAGCGCCGTGATGTCACGCCGTTCGAGCGCACCGCCGATCT
GGCCGAAGTCCTGAAAGTCGCCAACCCGGCGTGGGAAAAGGGCAAGAACCCGGCAACCCGCGCTTTCCAGGGCCTGCGCA
TTCAAGTCAACAACGAACTGGGCGATCTGGAAGCGGGTCTTGAAGCCGCGCTCGAATGCCTGGAAGTGGGCGGTCGTCTG
GTCGTGATCAGCTTCCACTCGCTGGAAGACCGTATTGTCAAACTGTTCATGCGCAAGCTGGTGAAAGGCGAAGCCGACAA
CCTGCCGCGCAACCTGCCGGTTCGCCACGTTGCGTTCGAACCGAAAATCAAAGTCCATGGCAAAGCGCAGACGGCCTCCG
ACGCCGAACTCAAAGCCAACCCACGTTCCCGTAGCGCCGTCATGCGCGTCGCGGAGAAGCTGCGGTGA

Upstream 100 bases:

>100_bases
AACTGTGGGACGAAGATGCCTGGAACGCGGTTTCTGCCGCTGACTTGGCTGCCATACAACAACCGGGCGCCATGCCTGAC
GAATTGCGTGATCTGATCCT

Downstream 100 bases:

>100_bases
GCAAGCTTTTCGCCAAGCCACTGCCCGGCGGCAGCTTTTTCATGCTGCTGCTGTTCATCGGCGTGCTCGTGTCGGCCATC
GGCGTGTCTTACAGCGCCCA

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MTIDSGFNHITVLLDEAVEALAVRPDGCYLDGTFGRGGHSRLILSKLGPDGRLIGFDKDPQAIATGQTLAAEDGRFVVVQ
RSFAELGSVVAERGLTGKVSGILLDLGVSSPQLDDAERGFSFLNDGPLDMRMDPSRGISAAEFVNTAPVEEIARVFKEYG
EERFSGRMARAVVERRDVTPFERTADLAEVLKVANPAWEKGKNPATRAFQGLRIQVNNELGDLEAGLEAALECLEVGGRL
VVISFHSLEDRIVKLFMRKLVKGEADNLPRNLPVRHVAFEPKIKVHGKAQTASDAELKANPRSRSAVMRVAEKLR

Sequences:

>Translated_315_residues
MTIDSGFNHITVLLDEAVEALAVRPDGCYLDGTFGRGGHSRLILSKLGPDGRLIGFDKDPQAIATGQTLAAEDGRFVVVQ
RSFAELGSVVAERGLTGKVSGILLDLGVSSPQLDDAERGFSFLNDGPLDMRMDPSRGISAAEFVNTAPVEEIARVFKEYG
EERFSGRMARAVVERRDVTPFERTADLAEVLKVANPAWEKGKNPATRAFQGLRIQVNNELGDLEAGLEAALECLEVGGRL
VVISFHSLEDRIVKLFMRKLVKGEADNLPRNLPVRHVAFEPKIKVHGKAQTASDAELKANPRSRSAVMRVAEKLR
>Mature_314_residues
TIDSGFNHITVLLDEAVEALAVRPDGCYLDGTFGRGGHSRLILSKLGPDGRLIGFDKDPQAIATGQTLAAEDGRFVVVQR
SFAELGSVVAERGLTGKVSGILLDLGVSSPQLDDAERGFSFLNDGPLDMRMDPSRGISAAEFVNTAPVEEIARVFKEYGE
ERFSGRMARAVVERRDVTPFERTADLAEVLKVANPAWEKGKNPATRAFQGLRIQVNNELGDLEAGLEAALECLEVGGRLV
VISFHSLEDRIVKLFMRKLVKGEADNLPRNLPVRHVAFEPKIKVHGKAQTASDAELKANPRSRSAVMRVAEKLR

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=340, Percent_Identity=37.9411764705882, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI165377202, Length=190, Percent_Identity=40, Blast_Score=125, Evalue=7e-29,
Organism=Escherichia coli, GI1786270, Length=311, Percent_Identity=56.2700964630225, Blast_Score=339, Evalue=1e-94,
Organism=Drosophila melanogaster, GI62472493, Length=329, Percent_Identity=36.7781155015198, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI28571637, Length=329, Percent_Identity=36.7781155015198, Blast_Score=186, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_PSEPF (Q3K736)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_350409.1
- ProteinModelPortal:   Q3K736
- SMR:   Q3K736
- STRING:   Q3K736
- GeneID:   3714581
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_4681
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- BioCyc:   PFLU205922:PFL_4681-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 34330; Mature: 34198

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: BINDING 57-57 BINDING 83-83 BINDING 105-105 BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIDSGFNHITVLLDEAVEALAVRPDGCYLDGTFGRGGHSRLILSKLGPDGRLIGFDKDP
CCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEEEECCCCCCCEECCCCCC
QAIATGQTLAAEDGRFVVVQRSFAELGSVVAERGLTGKVSGILLDLGVSSPQLDDAERGF
CCEECCCEEECCCCCEEEEEHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHHCC
SFLNDGPLDMRMDPSRGISAAEFVNTAPVEEIARVFKEYGEERFSGRMARAVVERRDVTP
HHCCCCCCCEEECCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
FERTADLAEVLKVANPAWEKGKNPATRAFQGLRIQVNNELGDLEAGLEAALECLEVGGRL
HHHHHHHHHHHHHCCCHHHCCCCHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHCCCEE
VVISFHSLEDRIVKLFMRKLVKGEADNLPRNLPVRHVAFEPKIKVHGKAQTASDAELKAN
EEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCEEECCCCCCCCCCCCCCC
PRSRSAVMRVAEKLR
CCHHHHHHHHHHHCC
>Mature Secondary Structure 
TIDSGFNHITVLLDEAVEALAVRPDGCYLDGTFGRGGHSRLILSKLGPDGRLIGFDKDP
CCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEEEECCCCCCCEECCCCCC
QAIATGQTLAAEDGRFVVVQRSFAELGSVVAERGLTGKVSGILLDLGVSSPQLDDAERGF
CCEECCCEEECCCCCEEEEEHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHHCC
SFLNDGPLDMRMDPSRGISAAEFVNTAPVEEIARVFKEYGEERFSGRMARAVVERRDVTP
HHCCCCCCCEEECCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
FERTADLAEVLKVANPAWEKGKNPATRAFQGLRIQVNNELGDLEAGLEAALECLEVGGRL
HHHHHHHHHHHHHCCCHHHCCCCHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHCCCEE
VVISFHSLEDRIVKLFMRKLVKGEADNLPRNLPVRHVAFEPKIKVHGKAQTASDAELKAN
EEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCEEECCCCCCCCCCCCCCC
PRSRSAVMRVAEKLR
CCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA