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The map label for this gene is mcrA [C]

Identifier: 77460849

GI number: 77460849

Start: 5228434

End: 5229405

Strand: Direct

Name: mcrA [C]

Synonym: Pfl01_4628

Alternate gene names: 77460849

Gene position: 5228434-5229405 (Clockwise)

Preceding gene: 77460848

Following gene: 77460850

Centisome position: 81.21

GC content: 51.03

Gene sequence:

>972_bases
ATGGAACCGTCATCCCAGAAACGCGCCTTTCGTACCCCTGAAAGCATCCAAGCCGAAAGTGAAACCCGAGGAATGCTCAA
ACCATTCTTAGAAGGGAGGGGCTTCACCGAGGTTACTGACCGCCGTAAAGGGCATTCTCAATTGATCACGGCCACCAGTC
CTGAAGGTTTGAAGTTCAGCGCTCGGGTCAGATTGTGTTGGCGCTGGGAGGATCAACCGGAAAAATACTCCGCAGCACAG
TTACGCGCGCGGTTAAAAGATGGCGATTGGGATTTAACAATCGCTGAGCTGGTTGGTCGCGAAGCTACCCACGGGGTGAC
ACATGCGCTGCTGGTGCAGCGCTACAAGAGTGATATCAGGTTTGCTGCTTTGATACCTGTCAGCGCCATGACTGGCATCA
TCAACGAGCAGAAAAGAGTCAGCGCTAATCTCATCTCGGCGGGAAAATTAGGACGTCAAAAGAAAAACCATGCGGTCAAT
GGTAATAGTCCAACGATGTGGCTGATGGACAACCGCTCTCAAGAGGGACATCAAGTCTCTGATGTTCTATGGCGATGCGA
TGGAGTGGAGGACATCGCCAAGCGACCACTCACCGCTGATCTTATAGGGATTATCGACGACACCTTCAGTGATGTTGCTG
GCCTCGATTTATCGCTGTTAGGTCGCGACGAAGCTTCACGTTATCAAGCCACGGTATCTAGGGTAGCTAGAGACCCCAGC
GTTCGAAAAGCTGTCATTGACCGTTGCAACGGTCGTTGTGAGCGTCCTGGATGTGGAGCAAGCCGCTCCTACTCTGGTTT
TCTTGATGTACACCACATATTGGGTGTTGAGATAAGCGATCGCGTGGCCAACTGTGTTGCCCTTTGCCCAAATTGCCACC
GAGATGCACATTTTTCAGCGGATAGACAAAAGTTGAATGACTCGTTGCTGATCGTAGCTCAGAATCAGCCTGAGACCGTA
TATACGATTTAA

Upstream 100 bases:

>100_bases
CGAAAAAACGTAGGTTGCCTCAGGTCTCATATGCCTGTTGCTGCAACTGGAAAAGCATCTCCTCCGGCCTACTCATCAAC
TGCTACCTGGTATTTCATGA

Downstream 100 bases:

>100_bases
CAGATCGTCAGTAGGAGCTACGGTGTATCCCCCGCTTGTATCCCTTTGACAACAAATGCTCTGAAGACCAGATATTATAT
GGCGCAGTCGAGTCCCCCTC

Product: HNH endonuclease

Products: NA

Alternate protein names: HNH Endonuclease TypeIV Restriction

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MEPSSQKRAFRTPESIQAESETRGMLKPFLEGRGFTEVTDRRKGHSQLITATSPEGLKFSARVRLCWRWEDQPEKYSAAQ
LRARLKDGDWDLTIAELVGREATHGVTHALLVQRYKSDIRFAALIPVSAMTGIINEQKRVSANLISAGKLGRQKKNHAVN
GNSPTMWLMDNRSQEGHQVSDVLWRCDGVEDIAKRPLTADLIGIIDDTFSDVAGLDLSLLGRDEASRYQATVSRVARDPS
VRKAVIDRCNGRCERPGCGASRSYSGFLDVHHILGVEISDRVANCVALCPNCHRDAHFSADRQKLNDSLLIVAQNQPETV
YTI

Sequences:

>Translated_323_residues
MEPSSQKRAFRTPESIQAESETRGMLKPFLEGRGFTEVTDRRKGHSQLITATSPEGLKFSARVRLCWRWEDQPEKYSAAQ
LRARLKDGDWDLTIAELVGREATHGVTHALLVQRYKSDIRFAALIPVSAMTGIINEQKRVSANLISAGKLGRQKKNHAVN
GNSPTMWLMDNRSQEGHQVSDVLWRCDGVEDIAKRPLTADLIGIIDDTFSDVAGLDLSLLGRDEASRYQATVSRVARDPS
VRKAVIDRCNGRCERPGCGASRSYSGFLDVHHILGVEISDRVANCVALCPNCHRDAHFSADRQKLNDSLLIVAQNQPETV
YTI
>Mature_323_residues
MEPSSQKRAFRTPESIQAESETRGMLKPFLEGRGFTEVTDRRKGHSQLITATSPEGLKFSARVRLCWRWEDQPEKYSAAQ
LRARLKDGDWDLTIAELVGREATHGVTHALLVQRYKSDIRFAALIPVSAMTGIINEQKRVSANLISAGKLGRQKKNHAVN
GNSPTMWLMDNRSQEGHQVSDVLWRCDGVEDIAKRPLTADLIGIIDDTFSDVAGLDLSLLGRDEASRYQATVSRVARDPS
VRKAVIDRCNGRCERPGCGASRSYSGFLDVHHILGVEISDRVANCVALCPNCHRDAHFSADRQKLNDSLLIVAQNQPETV
YTI

Specific function: Restriction Of 5-Methyl And 5-Hydroxymethylcytosines At The Specific DNA Sequence C(Me)Cgg. [C]

COG id: COG1403

COG function: function code V; Restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787406, Length=111, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.1.21.- [C]

Molecular weight: Translated: 35951; Mature: 35951

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPSSQKRAFRTPESIQAESETRGMLKPFLEGRGFTEVTDRRKGHSQLITATSPEGLKFS
CCCCCCCHHCCCCHHHCCCHHHHHHHHHHHCCCCCHHHHHCCCCCCEEEEECCCCCCEEE
ARVRLCWRWEDQPEKYSAAQLRARLKDGDWDLTIAELVGREATHGVTHALLVQRYKSDIR
EEEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEHHHHHCCHHHHHHHHHHHHHHHHHCCE
FAALIPVSAMTGIINEQKRVSANLISAGKLGRQKKNHAVNGNSPTMWLMDNRSQEGHQVS
EEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCEEEEEECCCCCCHHHH
DVLWRCDGVEDIAKRPLTADLIGIIDDTFSDVAGLDLSLLGRDEASRYQATVSRVARDPS
HHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCCHH
VRKAVIDRCNGRCERPGCGASRSYSGFLDVHHILGVEISDRVANCVALCPNCHRDAHFSA
HHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCH
DRQKLNDSLLIVAQNQPETVYTI
HHHHCCCCEEEEECCCCCEEEEC
>Mature Secondary Structure
MEPSSQKRAFRTPESIQAESETRGMLKPFLEGRGFTEVTDRRKGHSQLITATSPEGLKFS
CCCCCCCHHCCCCHHHCCCHHHHHHHHHHHCCCCCHHHHHCCCCCCEEEEECCCCCCEEE
ARVRLCWRWEDQPEKYSAAQLRARLKDGDWDLTIAELVGREATHGVTHALLVQRYKSDIR
EEEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEHHHHHCCHHHHHHHHHHHHHHHHHCCE
FAALIPVSAMTGIINEQKRVSANLISAGKLGRQKKNHAVNGNSPTMWLMDNRSQEGHQVS
EEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCEEEEEECCCCCCHHHH
DVLWRCDGVEDIAKRPLTADLIGIIDDTFSDVAGLDLSLLGRDEASRYQATVSRVARDPS
HHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCCHH
VRKAVIDRCNGRCERPGCGASRSYSGFLDVHHILGVEISDRVANCVALCPNCHRDAHFSA
HHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCH
DRQKLNDSLLIVAQNQPETVYTI
HHHHCCCCEEEEECCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA