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The map label for this gene is queA [H]

Identifier: 77460843

GI number: 77460843

Start: 5220664

End: 5221713

Strand: Reverse

Name: queA [H]

Synonym: Pfl01_4622

Alternate gene names: 77460843

Gene position: 5221713-5220664 (Counterclockwise)

Preceding gene: 77460852

Following gene: 77460842

Centisome position: 81.1

GC content: 62.48

Gene sequence:

>1050_bases
ATGCGCGTTGCTGACTTTACCTTCGAGCTTCCTGATTCGCTGATTGCCCGCCATCCTTTGGCCGAGCGTCGCGGTAGTCG
CCTGTTGACCCTGGATGGGGTCAGCGGCGCCCTGGCACACCGTCAATTCACTGATCTGCTGGAGCATTTGCGCCCCGGCG
ATCTGATGGTGTTCAACAATACCCGGGTGATTCCGGCGCGGCTTTTCGGGCAGAAGGCGTCCGGCGGCAAGCTGGAAATC
CTTGTCGAGCGCGTGCTCGATACCCACCGCGTGCTGGCTCATGTGCGTTCCAGCAAGTCGCCGAAACCGGGCTCGAAGAT
CCTGATCGACGGCGGCGGCGAAGCCGAGATGCTGGCGCGTCACGATGCGCTGTTCGAGCTGGGGTTTGCCGAAGAAGTGC
TGCCGCTGCTCGATCGCGTCGGGCACATGCCGTTGCCTCCTTATATAGACCGCCCGGACGAAGGTTCGGATCGCGAGCGC
TATCAGACCGTGTATGCCGAGAAGCTCGGCGCGGTGGCGGCGCCGACTGCCGGGCTGCATTTCGATCAGACGCTGATGGA
AGCGATCTCCGCCAAGGGCGTCGAGACGGCGTTCGTCACGCTGCACGTCGGCGCCGGCACCTTCCAGCCGGTGCGCGTGG
AGAAGATCGAAGATCACCACATGCACACCGAGTGGCTGGAAGTCGGTCAGGATGTGGTCGATGCCGTCGCCGCTTGCCGT
GAGCGTGGCGGTCGCGTCATCGCTGTCGGCACCACCAGCGTGCGTTCGCTGGAAAGCGCCGCCCGCGATGGCGTACTTAA
ACCGTTCAGTGGCGACACCGACATCTTTATCTACCCGGGCCGGCCGTTCCATGTGGTCGACGCCCTGGTCACCAATTTCC
ATTTGCCCGAGTCCACGCTGTTGATGCTGGTATCGGCGTTCGCCGGTTATCCGGAAACCATGGCTGCCTACAAAGCCGCG
GTCGATAACGGATACCGCTTTTTCAGCTACGGTGATGCGATGTTCATCACCCGTAATCCCGCGCCGCGCGGCCCAGAGGA
AACAGTATGA

Upstream 100 bases:

>100_bases
GGCGAAGTATAGAGAGGTGCCTACCGTTGGTCAATCACGTTTCATGGTCAATTTTCGATATTTCCGCTAGACTTTCCGGC
CCTGCTAGACGAACCCCATC

Downstream 100 bases:

>100_bases
GTCGCACCTGTCGTATGTCGTTCGAGCTGCTTGCCACCGATGGCAAGGCCCGTCGTGGTCGTCTGACCTTCCCCCGTGGC
ACCGTCGAGACCCCGGCCTT

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MRVADFTFELPDSLIARHPLAERRGSRLLTLDGVSGALAHRQFTDLLEHLRPGDLMVFNNTRVIPARLFGQKASGGKLEI
LVERVLDTHRVLAHVRSSKSPKPGSKILIDGGGEAEMLARHDALFELGFAEEVLPLLDRVGHMPLPPYIDRPDEGSDRER
YQTVYAEKLGAVAAPTAGLHFDQTLMEAISAKGVETAFVTLHVGAGTFQPVRVEKIEDHHMHTEWLEVGQDVVDAVAACR
ERGGRVIAVGTTSVRSLESAARDGVLKPFSGDTDIFIYPGRPFHVVDALVTNFHLPESTLLMLVSAFAGYPETMAAYKAA
VDNGYRFFSYGDAMFITRNPAPRGPEETV

Sequences:

>Translated_349_residues
MRVADFTFELPDSLIARHPLAERRGSRLLTLDGVSGALAHRQFTDLLEHLRPGDLMVFNNTRVIPARLFGQKASGGKLEI
LVERVLDTHRVLAHVRSSKSPKPGSKILIDGGGEAEMLARHDALFELGFAEEVLPLLDRVGHMPLPPYIDRPDEGSDRER
YQTVYAEKLGAVAAPTAGLHFDQTLMEAISAKGVETAFVTLHVGAGTFQPVRVEKIEDHHMHTEWLEVGQDVVDAVAACR
ERGGRVIAVGTTSVRSLESAARDGVLKPFSGDTDIFIYPGRPFHVVDALVTNFHLPESTLLMLVSAFAGYPETMAAYKAA
VDNGYRFFSYGDAMFITRNPAPRGPEETV
>Mature_349_residues
MRVADFTFELPDSLIARHPLAERRGSRLLTLDGVSGALAHRQFTDLLEHLRPGDLMVFNNTRVIPARLFGQKASGGKLEI
LVERVLDTHRVLAHVRSSKSPKPGSKILIDGGGEAEMLARHDALFELGFAEEVLPLLDRVGHMPLPPYIDRPDEGSDRER
YQTVYAEKLGAVAAPTAGLHFDQTLMEAISAKGVETAFVTLHVGAGTFQPVRVEKIEDHHMHTEWLEVGQDVVDAVAACR
ERGGRVIAVGTTSVRSLESAARDGVLKPFSGDTDIFIYPGRPFHVVDALVTNFHLPESTLLMLVSAFAGYPETMAAYKAA
VDNGYRFFSYGDAMFITRNPAPRGPEETV

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=347, Percent_Identity=70.6051873198847, Blast_Score=490, Evalue=1e-140,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38278; Mature: 38278

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVADFTFELPDSLIARHPLAERRGSRLLTLDGVSGALAHRQFTDLLEHLRPGDLMVFNN
CCCCCEEEECCHHHHHHCCHHHHCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEECC
TRVIPARLFGQKASGGKLEILVERVLDTHRVLAHVRSSKSPKPGSKILIDGGGEAEMLAR
CEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHH
HDALFELGFAEEVLPLLDRVGHMPLPPYIDRPDEGSDRERYQTVYAEKLGAVAAPTAGLH
HHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
FDQTLMEAISAKGVETAFVTLHVGAGTFQPVRVEKIEDHHMHTEWLEVGQDVVDAVAACR
HHHHHHHHHHCCCCEEEEEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
ERGGRVIAVGTTSVRSLESAARDGVLKPFSGDTDIFIYPGRPFHVVDALVTNFHLPESTL
HCCCEEEEEECHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCHHHH
LMLVSAFAGYPETMAAYKAAVDNGYRFFSYGDAMFITRNPAPRGPEETV
HHHHHHHCCCHHHHHHHHHHHCCCEEEEEECCEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MRVADFTFELPDSLIARHPLAERRGSRLLTLDGVSGALAHRQFTDLLEHLRPGDLMVFNN
CCCCCEEEECCHHHHHHCCHHHHCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEECC
TRVIPARLFGQKASGGKLEILVERVLDTHRVLAHVRSSKSPKPGSKILIDGGGEAEMLAR
CEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHH
HDALFELGFAEEVLPLLDRVGHMPLPPYIDRPDEGSDRERYQTVYAEKLGAVAAPTAGLH
HHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
FDQTLMEAISAKGVETAFVTLHVGAGTFQPVRVEKIEDHHMHTEWLEVGQDVVDAVAACR
HHHHHHHHHHCCCCEEEEEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
ERGGRVIAVGTTSVRSLESAARDGVLKPFSGDTDIFIYPGRPFHVVDALVTNFHLPESTL
HCCCEEEEEECHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCHHHH
LMLVSAFAGYPETMAAYKAAVDNGYRFFSYGDAMFITRNPAPRGPEETV
HHHHHHHCCCHHHHHHHHHHHCCCEEEEEECCEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA