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The map label for this gene is trmJ [H]

Identifier: 77460836

GI number: 77460836

Start: 5213793

End: 5214587

Strand: Reverse

Name: trmJ [H]

Synonym: Pfl01_4615

Alternate gene names: 77460836

Gene position: 5214587-5213793 (Counterclockwise)

Preceding gene: 77460838

Following gene: 77460835

Centisome position: 80.99

GC content: 61.38

Gene sequence:

>795_bases
TTGCCGAGTCCGAGAGCGCCCGCCGTGCTGCAAAATATTCGTGTCGTCCTGGTCAACACCAGCCATCCGGGAAACATCGG
CGGGACCGCGCGCGCCATGAAAAACATGGGCCTGTCGCGGCTGGTGCTGGTTGATCCGCGAGTCTTCCCGCATCACGAAG
CCGATGCCCGTGCTTCCGGTGCCGGTGACATCCTTGAAAACGCGCAAGTCGTCGCCACTTTGGAAGACGCCTTGGTCGGT
TGCAATCTGGTGCTCGGCACCAGTGCCCGTGACCGGCGCATTCCCTGGCCATTGCTGGATCCGCGCGAGTGCGGGACCAA
AGTGGTCGAGGAGGCCGGGCAGGGCGCGGAAATCGCGCTGGTGTTCGGTCGTGAGGATTCCGGCCTGACCAATGACGAGC
TGCAGCGATGTCACTATCACGTGCACATCCCCTCCGATCCTGAATTCAGCTCGCTGAACCTCGGGGCGGCGGTGCAGGTG
TTGAGTTATGAAGTGCGCATGGCGTGGCTGGCTGCACAAGGCCAGCCGACCAAGATCGAGAAGGAAGAAGTGGCCTCCGT
GAAAAGCGCAGAGCTTGCGACCATGGATGAGCTGGAGCGGTTCTATGAACACCTGGAGCAGACCCTGGTGGCCATCGAAT
TCCTCGATCCGGAAAAGCCGCGGCACTTGATGGCGCGCCTGCGCCGGTTGTATGGACGCAGCTCGGTCAGCCGGGCGGAA
ATGAATATTTTGCGTGGCATCCTCACGGAAACCCAGAAAGCGGCCCGTGGCGAGCTCCTTAAGCGGAAGGATTAA

Upstream 100 bases:

>100_bases
TAAAGAAAGCCGCGCATTCTATCAGAACTTTTCTTCAGGTGAAGGTCGCTGTTCGCTTTCATAGCTTAACGGTAGGCTGT
TCTGTAAGATTTGCACCCCT

Downstream 100 bases:

>100_bases
TGATGTTCGAGCGTTTGCGTGAAGATATCCAGAGCGTATTCCACCGAGACCCGGCGGCGCGTAACGCTTTTGAAGTCCTG
ACCTGCTACCCCGGCATGCA

Product: RNA methyltransferase TrmH

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MPSPRAPAVLQNIRVVLVNTSHPGNIGGTARAMKNMGLSRLVLVDPRVFPHHEADARASGAGDILENAQVVATLEDALVG
CNLVLGTSARDRRIPWPLLDPRECGTKVVEEAGQGAEIALVFGREDSGLTNDELQRCHYHVHIPSDPEFSSLNLGAAVQV
LSYEVRMAWLAAQGQPTKIEKEEVASVKSAELATMDELERFYEHLEQTLVAIEFLDPEKPRHLMARLRRLYGRSSVSRAE
MNILRGILTETQKAARGELLKRKD

Sequences:

>Translated_264_residues
MPSPRAPAVLQNIRVVLVNTSHPGNIGGTARAMKNMGLSRLVLVDPRVFPHHEADARASGAGDILENAQVVATLEDALVG
CNLVLGTSARDRRIPWPLLDPRECGTKVVEEAGQGAEIALVFGREDSGLTNDELQRCHYHVHIPSDPEFSSLNLGAAVQV
LSYEVRMAWLAAQGQPTKIEKEEVASVKSAELATMDELERFYEHLEQTLVAIEFLDPEKPRHLMARLRRLYGRSSVSRAE
MNILRGILTETQKAARGELLKRKD
>Mature_263_residues
PSPRAPAVLQNIRVVLVNTSHPGNIGGTARAMKNMGLSRLVLVDPRVFPHHEADARASGAGDILENAQVVATLEDALVGC
NLVLGTSARDRRIPWPLLDPRECGTKVVEEAGQGAEIALVFGREDSGLTNDELQRCHYHVHIPSDPEFSSLNLGAAVQVL
SYEVRMAWLAAQGQPTKIEKEEVASVKSAELATMDELERFYEHLEQTLVAIEFLDPEKPRHLMARLRRLYGRSSVSRAEM
NILRGILTETQKAARGELLKRKD

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=249, Percent_Identity=55.8232931726908, Blast_Score=280, Evalue=1e-76,
Organism=Escherichia coli, GI1790865, Length=155, Percent_Identity=40, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 29126; Mature: 28995

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSPRAPAVLQNIRVVLVNTSHPGNIGGTARAMKNMGLSRLVLVDPRVFPHHEADARASG
CCCCCCCHHHCCCEEEEEECCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCC
AGDILENAQVVATLEDALVGCNLVLGTSARDRRIPWPLLDPRECGTKVVEEAGQGAEIAL
CCHHHHCCCCEEEHHHHHHHCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEE
VFGREDSGLTNDELQRCHYHVHIPSDPEFSSLNLGAAVQVLSYEVRMAWLAAQGQPTKIE
EECCCCCCCCHHHHHHCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHEEEECCCCCCCCC
KEEVASVKSAELATMDELERFYEHLEQTLVAIEFLDPEKPRHLMARLRRLYGRSSVSRAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHH
MNILRGILTETQKAARGELLKRKD
HHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PSPRAPAVLQNIRVVLVNTSHPGNIGGTARAMKNMGLSRLVLVDPRVFPHHEADARASG
CCCCCCHHHCCCEEEEEECCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCC
AGDILENAQVVATLEDALVGCNLVLGTSARDRRIPWPLLDPRECGTKVVEEAGQGAEIAL
CCHHHHCCCCEEEHHHHHHHCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEE
VFGREDSGLTNDELQRCHYHVHIPSDPEFSSLNLGAAVQVLSYEVRMAWLAAQGQPTKIE
EECCCCCCCCHHHHHHCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHEEEECCCCCCCCC
KEEVASVKSAELATMDELERFYEHLEQTLVAIEFLDPEKPRHLMARLRRLYGRSSVSRAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHH
MNILRGILTETQKAARGELLKRKD
HHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA