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The map label for this gene is leuA [H]

Identifier: 77460815

GI number: 77460815

Start: 5194022

End: 5195701

Strand: Direct

Name: leuA [H]

Synonym: Pfl01_4594

Alternate gene names: 77460815

Gene position: 5194022-5195701 (Clockwise)

Preceding gene: 77460814

Following gene: 77460837

Centisome position: 80.67

GC content: 61.85

Gene sequence:

>1680_bases
ATGAGCATGCTCAAAGACCCGTCTTCGAAATACCGTGCGTTTCCAGTGATCAACCTGCCGGATCGCACCTGGCCGTCGAA
AACCATCGATGCCGCGCCAATCTGGTGCAGCTCCGACCTGCGTGACGGCAACCAGTCGTTGATCGAGCCAATGGACGCGG
TGAAAAAGCTGCGCTTCTGGAAAACCCTGGTGCAAGTGGGCGTGAAGGAAATCGAAGCGTCGTTCCCGGCCGCTTCGCAA
ACCGACTTCGACTTCGTGCGTACCCTGATCGAGGACGGCCACATCCCGGACGACACCACCATTCAGGTGCTGACCCAGGG
CCGTGAAGACCTGATCGAGCGCACCTTCGAATCCCTGCGCGGGGCGAAGAAAGCCATCGTTCATCTGTACAACGCCACCT
CCCCTTCTTTCCGTCGCATCGTTTTCAACCAGGACAAGGACGGGATCAAGGCCATCGCCGTGAACGCCGCCAAGCTGTTC
GTCAAATACGCGGCCATGCAGCCGGACACCGAGTGGACCTTCGAGTACTCGCCGGAAACCTTCAGCGCCACCGAACTGGA
ATTCGCCAAGGAAGTCTGTGACGCGGTGATCGAGGTCTGGAACCCGACGCCTGAGCACAAGATCATCCTCAACCTGCCGG
CCACCGTTGAATGTGCGACCCCGAACGTCTACGCCGACCAGATCGAGTGGTTCCACCGCAACATCAACCGCCGTGACAGC
GTGATCATCAGCCTGCACACCCACAACGACCGCGGCACTGGCGTGGCCGCCACCGAGCTGGGCCTGATGGCCGGCGCCGA
TCGCGTCGAAGGCTGCCTGTTCGGCAACGGCGAGCGTACCGGTAACGTCGACCTCGTGACTGTGGCACTGAACATGTACA
CCCAGGGCATCAATCCTGACCTGGACTTTTCCGACATCGACGGCGTGCGCAAAGTCGTCGAAGAGTGCAACCAGATCCAG
GTTCACCCGCGTCACCCGTACGTCGGCGACCTGGTGCACACAGCGTTCTCCGGCTCCCACCAGGACGCGATCCGCAAGGG
CTTCGCCCAGCAGAAACCGGACACCCTGTGGGAAGTGCCGTACCTGCCGATCGACCCGGCCGACATCGGCCGCAGCTACG
AGGCGGTGATCCGCGTCAACAGCCAGTCGGGCAAGGGCGGCATCGCCTACCTGCTGGAGCAGGAATACGGCATCAGCCTG
CCACGTCGCATGCAGATCGAATTCAGCCAGGTCGTGCAGCGTGAAACCGATCGCCTGGGCCTGGAGATGACCGCGCAGCA
AATCCACGCGCTGCTGCACAGCGAATATCTGCAAGCCAACACCCCGTACGCGCTGGTCAGCCATCGCCTGCAGGAAGAAA
ACGGTAACAGCGCCGTCGAAGTGGAAGTGGCGAGCAAAGGTCAGGGCGAAACCAATCTGCACTGGCGCGGCAAGGGCAAC
GGCGCGCTGGAAGCCCTGGTGGCCGGTCTGCCGATTCCGGTGGAAATCATGGACTACAACGAACACGCCATCGGCGCGGG
CACTAACGCCAAGGCGGCAGCCTACATCGAGCTGCGTGTGAACGGCGAACGTGCGGTGCACGGCGTGGGCATCGATGAAA
ACATCACCACCGCCAGCTTCAAGGCACTGTTCAGCGCGCTGAACCGCTCGCTGAGCCAGCCGGAAGCGAAAGCGGCGTAA

Upstream 100 bases:

>100_bases
TGCCGGGCTGAATCGCTGCACCTCGTCCCACGCTTTTCCAAGAACGTTCGTTCCACCGGCAGGCCGCCTCTTTTCGGCCC
AGACAACAAGGAATTTTCCG

Downstream 100 bases:

>100_bases
CTGACCGCTGCAATGCAAAAGGCCCCGGGGTTTGCACCTCGGGGCCTTTTTATTGCTTCTTCTGATCGTTCCCACGCAGA
GCATGGGAACGATCAGGCGC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 559; Mature: 558

Protein sequence:

>559_residues
MSMLKDPSSKYRAFPVINLPDRTWPSKTIDAAPIWCSSDLRDGNQSLIEPMDAVKKLRFWKTLVQVGVKEIEASFPAASQ
TDFDFVRTLIEDGHIPDDTTIQVLTQGREDLIERTFESLRGAKKAIVHLYNATSPSFRRIVFNQDKDGIKAIAVNAAKLF
VKYAAMQPDTEWTFEYSPETFSATELEFAKEVCDAVIEVWNPTPEHKIILNLPATVECATPNVYADQIEWFHRNINRRDS
VIISLHTHNDRGTGVAATELGLMAGADRVEGCLFGNGERTGNVDLVTVALNMYTQGINPDLDFSDIDGVRKVVEECNQIQ
VHPRHPYVGDLVHTAFSGSHQDAIRKGFAQQKPDTLWEVPYLPIDPADIGRSYEAVIRVNSQSGKGGIAYLLEQEYGISL
PRRMQIEFSQVVQRETDRLGLEMTAQQIHALLHSEYLQANTPYALVSHRLQEENGNSAVEVEVASKGQGETNLHWRGKGN
GALEALVAGLPIPVEIMDYNEHAIGAGTNAKAAAYIELRVNGERAVHGVGIDENITTASFKALFSALNRSLSQPEAKAA

Sequences:

>Translated_559_residues
MSMLKDPSSKYRAFPVINLPDRTWPSKTIDAAPIWCSSDLRDGNQSLIEPMDAVKKLRFWKTLVQVGVKEIEASFPAASQ
TDFDFVRTLIEDGHIPDDTTIQVLTQGREDLIERTFESLRGAKKAIVHLYNATSPSFRRIVFNQDKDGIKAIAVNAAKLF
VKYAAMQPDTEWTFEYSPETFSATELEFAKEVCDAVIEVWNPTPEHKIILNLPATVECATPNVYADQIEWFHRNINRRDS
VIISLHTHNDRGTGVAATELGLMAGADRVEGCLFGNGERTGNVDLVTVALNMYTQGINPDLDFSDIDGVRKVVEECNQIQ
VHPRHPYVGDLVHTAFSGSHQDAIRKGFAQQKPDTLWEVPYLPIDPADIGRSYEAVIRVNSQSGKGGIAYLLEQEYGISL
PRRMQIEFSQVVQRETDRLGLEMTAQQIHALLHSEYLQANTPYALVSHRLQEENGNSAVEVEVASKGQGETNLHWRGKGN
GALEALVAGLPIPVEIMDYNEHAIGAGTNAKAAAYIELRVNGERAVHGVGIDENITTASFKALFSALNRSLSQPEAKAA
>Mature_558_residues
SMLKDPSSKYRAFPVINLPDRTWPSKTIDAAPIWCSSDLRDGNQSLIEPMDAVKKLRFWKTLVQVGVKEIEASFPAASQT
DFDFVRTLIEDGHIPDDTTIQVLTQGREDLIERTFESLRGAKKAIVHLYNATSPSFRRIVFNQDKDGIKAIAVNAAKLFV
KYAAMQPDTEWTFEYSPETFSATELEFAKEVCDAVIEVWNPTPEHKIILNLPATVECATPNVYADQIEWFHRNINRRDSV
IISLHTHNDRGTGVAATELGLMAGADRVEGCLFGNGERTGNVDLVTVALNMYTQGINPDLDFSDIDGVRKVVEECNQIQV
HPRHPYVGDLVHTAFSGSHQDAIRKGFAQQKPDTLWEVPYLPIDPADIGRSYEAVIRVNSQSGKGGIAYLLEQEYGISLP
RRMQIEFSQVVQRETDRLGLEMTAQQIHALLHSEYLQANTPYALVSHRLQEENGNSAVEVEVASKGQGETNLHWRGKGNG
ALEALVAGLPIPVEIMDYNEHAIGAGTNAKAAAYIELRVNGERAVHGVGIDENITTASFKALFSALNRSLSQPEAKAA

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=529, Percent_Identity=24.5746691871456, Blast_Score=111, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324225, Length=567, Percent_Identity=52.0282186948854, Blast_Score=569, Evalue=1e-163,
Organism=Saccharomyces cerevisiae, GI6324682, Length=571, Percent_Identity=50.6129597197898, Blast_Score=568, Evalue=1e-162,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 61937; Mature: 61806

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMLKDPSSKYRAFPVINLPDRTWPSKTIDAAPIWCSSDLRDGNQSLIEPMDAVKKLRFW
CCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHH
KTLVQVGVKEIEASFPAASQTDFDFVRTLIEDGHIPDDTTIQVLTQGREDLIERTFESLR
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHHHHH
GAKKAIVHLYNATSPSFRRIVFNQDKDGIKAIAVNAAKLFVKYAAMQPDTEWTFEYSPET
HHHHHHEEEECCCCCCEEEEEECCCCCCCEEEEHHHHHHHHHHHHCCCCCCEEEEECCCC
FSATELEFAKEVCDAVIEVWNPTPEHKIILNLPATVECATPNVYADQIEWFHRNINRRDS
CCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEECCCCCHHHHHHHHHHCCCCCCC
VIISLHTHNDRGTGVAATELGLMAGADRVEGCLFGNGERTGNVDLVTVALNMYTQGINPD
EEEEEEECCCCCCCEEHHHHHHHCCCHHHCEEEECCCCCCCCEEEEEEEEEHHHCCCCCC
LDFSDIDGVRKVVEECNQIQVHPRHPYVGDLVHTAFSGSHQDAIRKGFAQQKPDTLWEVP
CCCHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEECC
YLPIDPADIGRSYEAVIRVNSQSGKGGIAYLLEQEYGISLPRRMQIEFSQVVQRETDRLG
CCCCCHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCEEEHHHHHHHHHHHHHCC
LEMTAQQIHALLHSEYLQANTPYALVSHRLQEENGNSAVEVEVASKGQGETNLHWRGKGN
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEECCCC
GALEALVAGLPIPVEIMDYNEHAIGAGTNAKAAAYIELRVNGERAVHGVGIDENITTASF
HHHHHHHHCCCCCEEEEECCCCEECCCCCCCEEEEEEEEECCCEEEEECCCCCCCCHHHH
KALFSALNRSLSQPEAKAA
HHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SMLKDPSSKYRAFPVINLPDRTWPSKTIDAAPIWCSSDLRDGNQSLIEPMDAVKKLRFW
CCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHH
KTLVQVGVKEIEASFPAASQTDFDFVRTLIEDGHIPDDTTIQVLTQGREDLIERTFESLR
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHHHHH
GAKKAIVHLYNATSPSFRRIVFNQDKDGIKAIAVNAAKLFVKYAAMQPDTEWTFEYSPET
HHHHHHEEEECCCCCCEEEEEECCCCCCCEEEEHHHHHHHHHHHHCCCCCCEEEEECCCC
FSATELEFAKEVCDAVIEVWNPTPEHKIILNLPATVECATPNVYADQIEWFHRNINRRDS
CCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEECCCCCHHHHHHHHHHCCCCCCC
VIISLHTHNDRGTGVAATELGLMAGADRVEGCLFGNGERTGNVDLVTVALNMYTQGINPD
EEEEEEECCCCCCCEEHHHHHHHCCCHHHCEEEECCCCCCCCEEEEEEEEEHHHCCCCCC
LDFSDIDGVRKVVEECNQIQVHPRHPYVGDLVHTAFSGSHQDAIRKGFAQQKPDTLWEVP
CCCHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEECC
YLPIDPADIGRSYEAVIRVNSQSGKGGIAYLLEQEYGISLPRRMQIEFSQVVQRETDRLG
CCCCCHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCEEEHHHHHHHHHHHHHCC
LEMTAQQIHALLHSEYLQANTPYALVSHRLQEENGNSAVEVEVASKGQGETNLHWRGKGN
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEECCCC
GALEALVAGLPIPVEIMDYNEHAIGAGTNAKAAAYIELRVNGERAVHGVGIDENITTASF
HHHHHHHHCCCCCEEEEECCCCEECCCCCCCEEEEEEEEECCCEEEEECCCCCCCCHHHH
KALFSALNRSLSQPEAKAA
HHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA