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The map label for this gene is nth [H]

Identifier: 77460734

GI number: 77460734

Start: 5088734

End: 5089372

Strand: Direct

Name: nth [H]

Synonym: Pfl01_4513

Alternate gene names: 77460734

Gene position: 5088734-5089372 (Clockwise)

Preceding gene: 77460733

Following gene: 77460735

Centisome position: 79.04

GC content: 56.65

Gene sequence:

>639_bases
ATGAATGCCGCAAAACGTCTGGAGATTTTCCGCAGGCTTCACGAGGACAACCCCGAGCCCAAGACCGAACTGGCCTACTC
CTCGCCCTTCGAACTATTGATTGCGGTGATTCTGTCCGCGCAATCCACGGACGTCGGGGTGAACAAGGCGACGGCGAAGC
TGTTTCCGGTGGCGAACACACCGGCGGCCATTCACGCCTTGGGCGTCGAAGGGTTGTCCGAGTACATCAAGACTATCGGC
CTCTATAACAGCAAGGCCAAAAACGTGATCGAGACCTGCCGCCTGCTGGTCGAATTACACAACGGGGAAGTCCCGCAGAC
GCGTGAAGAACTGGAAGCCTTGCCCGGCGTTGGCCGCAAGACCGCCAACGTCGTGCTCAACACCGCGTTCCGCCAGTTGA
CCATGGCCGTGGACACCCACATCTTCCGGGTCAGCAACCGCACCGGCATTGCACCGGGTAAAAATGTGGTCGAGGTGGAA
AAGAAGCTGATGAAGTTTGTGCCAAAAGAATACCTGCTCGACTCGCACCACTGGCTCATTCTTCACGGACGCTATGTGTG
TCTGGCACGCAAACCCCGTTGTGGCAGCTGTCGGATTGAAGATCTGTGCGATTACAAGCACAAAACTTCTGACGATTGA

Upstream 100 bases:

>100_bases
CGACGCTGGCTCCCGGCGCCTTCATATTGCTTGGATTGTTACTGGCCGCCCGTCAGGCCTGGACTCGCCCTGCCGCCTTA
CCCGAGGAAACGCATCACCC

Downstream 100 bases:

>100_bases
GCGACTATTGGTTTTATTGATTTATCGATTGAAAAAATCTTTTTTACCATCTGCTGGAATATCGATATAAGGAGCGCCAA
AGGCAGTCTTAGCCTGGAGT

Product: DNA-(apurinic or apyrimidinic site) lyase / endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MNAAKRLEIFRRLHEDNPEPKTELAYSSPFELLIAVILSAQSTDVGVNKATAKLFPVANTPAAIHALGVEGLSEYIKTIG
LYNSKAKNVIETCRLLVELHNGEVPQTREELEALPGVGRKTANVVLNTAFRQLTMAVDTHIFRVSNRTGIAPGKNVVEVE
KKLMKFVPKEYLLDSHHWLILHGRYVCLARKPRCGSCRIEDLCDYKHKTSDD

Sequences:

>Translated_212_residues
MNAAKRLEIFRRLHEDNPEPKTELAYSSPFELLIAVILSAQSTDVGVNKATAKLFPVANTPAAIHALGVEGLSEYIKTIG
LYNSKAKNVIETCRLLVELHNGEVPQTREELEALPGVGRKTANVVLNTAFRQLTMAVDTHIFRVSNRTGIAPGKNVVEVE
KKLMKFVPKEYLLDSHHWLILHGRYVCLARKPRCGSCRIEDLCDYKHKTSDD
>Mature_212_residues
MNAAKRLEIFRRLHEDNPEPKTELAYSSPFELLIAVILSAQSTDVGVNKATAKLFPVANTPAAIHALGVEGLSEYIKTIG
LYNSKAKNVIETCRLLVELHNGEVPQTREELEALPGVGRKTANVVLNTAFRQLTMAVDTHIFRVSNRTGIAPGKNVVEVE
KKLMKFVPKEYLLDSHHWLILHGRYVCLARKPRCGSCRIEDLCDYKHKTSDD

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA dam

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=29.9435028248588, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1787920, Length=208, Percent_Identity=75.4807692307692, Blast_Score=328, Evalue=1e-91,
Organism=Escherichia coli, GI1789331, Length=129, Percent_Identity=31.0077519379845, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17554540, Length=182, Percent_Identity=31.3186813186813, Blast_Score=94, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6324530, Length=192, Percent_Identity=25, Blast_Score=69, Evalue=6e-13,
Organism=Drosophila melanogaster, GI45550361, Length=181, Percent_Identity=30.3867403314917, Blast_Score=101, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23718; Mature: 23718

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS00764 ENDONUCLEASE_III_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAAKRLEIFRRLHEDNPEPKTELAYSSPFELLIAVILSAQSTDVGVNKATAKLFPVANT
CCHHHHHHHHHHHHCCCCCCCHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHEEEECCCC
PAAIHALGVEGLSEYIKTIGLYNSKAKNVIETCRLLVELHNGEVPQTREELEALPGVGRK
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCH
TANVVLNTAFRQLTMAVDTHIFRVSNRTGIAPGKNVVEVEKKLMKFVPKEYLLDSHHWLI
HHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHCCHHHHCCCCCEEE
LHGRYVCLARKPRCGSCRIEDLCDYKHKTSDD
EECCEEEEECCCCCCCCCHHHHHCCCCCCCCC
>Mature Secondary Structure
MNAAKRLEIFRRLHEDNPEPKTELAYSSPFELLIAVILSAQSTDVGVNKATAKLFPVANT
CCHHHHHHHHHHHHCCCCCCCHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHEEEECCCC
PAAIHALGVEGLSEYIKTIGLYNSKAKNVIETCRLLVELHNGEVPQTREELEALPGVGRK
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCH
TANVVLNTAFRQLTMAVDTHIFRVSNRTGIAPGKNVVEVEKKLMKFVPKEYLLDSHHWLI
HHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHCCHHHHCCCCCEEE
LHGRYVCLARKPRCGSCRIEDLCDYKHKTSDD
EECCEEEEECCCCCCCCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]