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The map label for this gene is basS [H]

Identifier: 77460718

GI number: 77460718

Start: 5071892

End: 5073166

Strand: Reverse

Name: basS [H]

Synonym: Pfl01_4497

Alternate gene names: 77460718

Gene position: 5073166-5071892 (Counterclockwise)

Preceding gene: 77460719

Following gene: 77460717

Centisome position: 78.8

GC content: 60.71

Gene sequence:

>1275_bases
ATGGAGTTTAAGCAAAGCCTTGCCCAGCGGATCATCATCGCCTTTGCGTTGATGAGTGCGCTGGTGGCCGGGGCGTTTGC
CATGGGCATCGTCGCGACCGTGCATCTGGTCGAGGAAAAACTGATTTCCGCCGGGCTCGGCGGCGACCTGCAACGACTGC
TGTTGATGGACAATGTTTCGGACTGGAGCCACCGGCCGGAACCGGATCAGTTGTTCTACTTCAGCGGCGGGCCGGGCGAC
TTCGAGCTGCCCAAGGACCTGCGTCACCTGGATTCAGGTTTCCATGAAGTCTTTCGCGAGCAGCTGTCGTACCACGCGAT
GGTCGAGGTGGTCGACGGTCGCCGCTATGTGCTGCTGCAGGATCAAAGCGATTTCGAGGAGCGCGAGCGTGTGCTGTTCG
CCGTGGTGCTGGTGGGCTTTGTGCTCAGTCTGGCACTGGCGGTGTTCCTTGGCTGGGTGCTGGCGCGCAAGGTGATGGCC
CCGGTGGTGCGGCTGGCCCGTCAGGTACGTCATCGCGATCAGTTGCTCGGACTGGCGCCTCCGCTGGCGCCGGATTATGC
GGCAGACGAGGTGGGTGAACTGGCGGTGGCATTCGACGCTACGCTCGGGCGCCTGCGTCAGGCGCTGACGCGGGAGCGTT
TGTTCACCAGTGACGTCAGCCACGAATTGCGTACACCGCTGATGGTATTGGCCAGCTCCTGTGAACTGCTGCTGGAAAAT
CCAGGTATCGATCAGCGCGGCAAGGCTCAGGTTCAGCGCATTGCGCGGGCCAGTGAGGAAATGCGTGAACTGGTGCAGAC
CTTCCTGATGCTCGCCCGTGCCCAGCGTGAAGACGCAGGCGCTGCACCCCAGCATAATCTTTCGCAAGTGGCTGAGAGTT
TGCTGTGCCTGTGGCGCGAGCCCATCGAAGCCAAGGGCTTGCAGCTGATTTTCGAGCCGGGCAATCCGCCCGACACCCGT
TACAACGCGACGCTGCTCAATGCCGTGATGGGCAATTTACTGCGCAACGCCTTGCATTACACCGAGCAGGGGTTCATTCG
CCTGACGCTCAACGAGAATGGTTTCGTGGTCGAGGATTCGGGTGTAGGCATTCCCGAAGAAAAACGCGAAGCCATGTTCC
AGCCGTTTGTACGTGGCAACGAAAAACGCGGCGAAGGGCTGGGGTTGGGTCTGTCATTGGTTCAACGCATCTGCGAGAAC
CAGGGCTGGAGCGTCACCCTGAGCACGATGGAGCCCAATGGCTGTCGTTTCAAGGTCGATCTGGGCAAGCGCTGA

Upstream 100 bases:

>100_bases
AGCCATGTCCACCAGTTGCGCCAGGTGATCGACAAACCGTTCGCCAAGCCATTGCTGCACACCGTGCACGGCGTGGGATA
CCGCTTGGCCGAGGGCCGCG

Downstream 100 bases:

>100_bases
GAGCATTGAATCTCACACGAGAGTGCCGCCAACCTGTCTAATTCCTGTATAACCTTGAAATAGTTTGCCGATTTACATGA
CGTTTTTTTCACAAAGGGAT

Product: periplasmic sensor Signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MEFKQSLAQRIIIAFALMSALVAGAFAMGIVATVHLVEEKLISAGLGGDLQRLLLMDNVSDWSHRPEPDQLFYFSGGPGD
FELPKDLRHLDSGFHEVFREQLSYHAMVEVVDGRRYVLLQDQSDFEERERVLFAVVLVGFVLSLALAVFLGWVLARKVMA
PVVRLARQVRHRDQLLGLAPPLAPDYAADEVGELAVAFDATLGRLRQALTRERLFTSDVSHELRTPLMVLASSCELLLEN
PGIDQRGKAQVQRIARASEEMRELVQTFLMLARAQREDAGAAPQHNLSQVAESLLCLWREPIEAKGLQLIFEPGNPPDTR
YNATLLNAVMGNLLRNALHYTEQGFIRLTLNENGFVVEDSGVGIPEEKREAMFQPFVRGNEKRGEGLGLGLSLVQRICEN
QGWSVTLSTMEPNGCRFKVDLGKR

Sequences:

>Translated_424_residues
MEFKQSLAQRIIIAFALMSALVAGAFAMGIVATVHLVEEKLISAGLGGDLQRLLLMDNVSDWSHRPEPDQLFYFSGGPGD
FELPKDLRHLDSGFHEVFREQLSYHAMVEVVDGRRYVLLQDQSDFEERERVLFAVVLVGFVLSLALAVFLGWVLARKVMA
PVVRLARQVRHRDQLLGLAPPLAPDYAADEVGELAVAFDATLGRLRQALTRERLFTSDVSHELRTPLMVLASSCELLLEN
PGIDQRGKAQVQRIARASEEMRELVQTFLMLARAQREDAGAAPQHNLSQVAESLLCLWREPIEAKGLQLIFEPGNPPDTR
YNATLLNAVMGNLLRNALHYTEQGFIRLTLNENGFVVEDSGVGIPEEKREAMFQPFVRGNEKRGEGLGLGLSLVQRICEN
QGWSVTLSTMEPNGCRFKVDLGKR
>Mature_424_residues
MEFKQSLAQRIIIAFALMSALVAGAFAMGIVATVHLVEEKLISAGLGGDLQRLLLMDNVSDWSHRPEPDQLFYFSGGPGD
FELPKDLRHLDSGFHEVFREQLSYHAMVEVVDGRRYVLLQDQSDFEERERVLFAVVLVGFVLSLALAVFLGWVLARKVMA
PVVRLARQVRHRDQLLGLAPPLAPDYAADEVGELAVAFDATLGRLRQALTRERLFTSDVSHELRTPLMVLASSCELLLEN
PGIDQRGKAQVQRIARASEEMRELVQTFLMLARAQREDAGAAPQHNLSQVAESLLCLWREPIEAKGLQLIFEPGNPPDTR
YNATLLNAVMGNLLRNALHYTEQGFIRLTLNENGFVVEDSGVGIPEEKREAMFQPFVRGNEKRGEGLGLGLSLVQRICEN
QGWSVTLSTMEPNGCRFKVDLGKR

Specific function: Member of the two-component regulatory system BasS/BasR Autophosphorylates and activates BasR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790551, Length=309, Percent_Identity=32.3624595469256, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1789403, Length=312, Percent_Identity=30.1282051282051, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789808, Length=307, Percent_Identity=28.9902280130293, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1786783, Length=334, Percent_Identity=24.8502994011976, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1790346, Length=225, Percent_Identity=28.4444444444444, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1787894, Length=238, Percent_Identity=31.0924369747899, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI48994928, Length=234, Percent_Identity=25.6410256410256, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081816, Length=262, Percent_Identity=28.6259541984733, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786600, Length=244, Percent_Identity=28.6885245901639, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI145693157, Length=226, Percent_Identity=25.6637168141593, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87082128, Length=316, Percent_Identity=26.5822784810127, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790861, Length=302, Percent_Identity=27.1523178807947, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1790436, Length=217, Percent_Identity=25.3456221198157, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1787374, Length=298, Percent_Identity=25.8389261744966, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788393, Length=279, Percent_Identity=27.2401433691756, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1788279, Length=237, Percent_Identity=28.2700421940928, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1789149, Length=348, Percent_Identity=22.7011494252874, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 47313; Mature: 47313

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFKQSLAQRIIIAFALMSALVAGAFAMGIVATVHLVEEKLISAGLGGDLQRLLLMDNVS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
DWSHRPEPDQLFYFSGGPGDFELPKDLRHLDSGFHEVFREQLSYHAMVEVVDGRRYVLLQ
CCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEE
DQSDFEERERVLFAVVLVGFVLSLALAVFLGWVLARKVMAPVVRLARQVRHRDQLLGLAP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PLAPDYAADEVGELAVAFDATLGRLRQALTRERLFTSDVSHELRTPLMVLASSCELLLEN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PGIDQRGKAQVQRIARASEEMRELVQTFLMLARAQREDAGAAPQHNLSQVAESLLCLWRE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHC
PIEAKGLQLIFEPGNPPDTRYNATLLNAVMGNLLRNALHYTEQGFIRLTLNENGFVVEDS
CCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEECC
GVGIPEEKREAMFQPFVRGNEKRGEGLGLGLSLVQRICENQGWSVTLSTMEPNGCRFKVD
CCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEE
LGKR
CCCC
>Mature Secondary Structure
MEFKQSLAQRIIIAFALMSALVAGAFAMGIVATVHLVEEKLISAGLGGDLQRLLLMDNVS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
DWSHRPEPDQLFYFSGGPGDFELPKDLRHLDSGFHEVFREQLSYHAMVEVVDGRRYVLLQ
CCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEE
DQSDFEERERVLFAVVLVGFVLSLALAVFLGWVLARKVMAPVVRLARQVRHRDQLLGLAP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PLAPDYAADEVGELAVAFDATLGRLRQALTRERLFTSDVSHELRTPLMVLASSCELLLEN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PGIDQRGKAQVQRIARASEEMRELVQTFLMLARAQREDAGAAPQHNLSQVAESLLCLWRE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHC
PIEAKGLQLIFEPGNPPDTRYNATLLNAVMGNLLRNALHYTEQGFIRLTLNENGFVVEDS
CCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEECC
GVGIPEEKREAMFQPFVRGNEKRGEGLGLGLSLVQRICENQGWSVTLSTMEPNGCRFKVD
CCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEE
LGKR
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8282725; 7610040; 9278503 [H]