| Definition | Pseudomonas fluorescens Pf0-1 chromosome, complete genome. |
|---|---|
| Accession | NC_007492 |
| Length | 6,438,405 |
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The map label for this gene is echA8 [H]
Identifier: 77460670
GI number: 77460670
Start: 5010199
End: 5010948
Strand: Reverse
Name: echA8 [H]
Synonym: Pfl01_4449
Alternate gene names: 77460670
Gene position: 5010948-5010199 (Counterclockwise)
Preceding gene: 77460671
Following gene: 77460668
Centisome position: 77.83
GC content: 64.13
Gene sequence:
>750_bases ATGCCCGAAGCCATCCTGCTGCACCGCGAACGCGGTTTGCTGACCCTGCGCCTCAACCGCCCGGACAAGAAAAACGCCCT GACCCGCGCCATGTACAGCCAATTGGCCGATGCGCTCAAGCAGGCGAATCACGACCCCGAAATCAACGCCGTGCTGATCA CCGGCAGCGCCGAGTGCTTCACCGCCGGCAACGACATCGCCGACTTCATCCAACAACCGCCGAGCGACCTCGACAGCCCG GTGTTTCACTTCATGCTCAACTTGCTTGAATGCCGTAAACCGGTGATCGCCGCCGTGGCCGGGGCGGCGGTGGGGATTGG CACGACGCTGCTGTTGCATTGCGATCTGGTGTACGTGGCGCGGGATGCACGGTTACGCATGCCGTTCGTCAATCTAGGGT TGTGCCCCGAGTTTGGCTCCAGCCTGATCCTGCCGCGCTTGCTCGGGCAGGCCAAAGCCTCGGAGTTGCTGCTGCTCGGT GAAGGCTTCACAGGTGAGCAAGCCGCGCAATGGGGGATCGCCACCGAGGCGCTGGGCAGCGGCGAAGCCGCATTGACCAA GGCGCGGGAGATGGCGCTGCGGTTTGACGAGTTGCCGGCCGAGGCGGTGCGGATCAGCAAGCAATTGATGCGCGCGCCGG ATCGCGAGTTGATTCGCAAAGTGATCGAGGAGGAGGGCGCACTGTTCACCCAGCGGCTGCGCTCGCCGGAGGCGCTGGCG GCGTTGACGGGGTTCATCAAACGGCATTGA
Upstream 100 bases:
>100_bases CCAAGCGTCTGTTCCTGGCCGGACTGCCGCGCAATCAACTGCTGGCCGATGTCTTCCTGAGCCGTGGTTGAGCGCTGAAT TCTCAGACGCGAGACCTGCC
Downstream 100 bases:
>100_bases GATATCCGCCGACCGCTCCGCGCATCGATCGCCCCCGCACTCCGCGCATCGACCGCGGCCACACTGCGCGCACGGACCGC CGCCGCACTCCGCGCACCGA
Product: enoyl-CoA hydratase
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 249; Mature: 248
Protein sequence:
>249_residues MPEAILLHRERGLLTLRLNRPDKKNALTRAMYSQLADALKQANHDPEINAVLITGSAECFTAGNDIADFIQQPPSDLDSP VFHFMLNLLECRKPVIAAVAGAAVGIGTTLLLHCDLVYVARDARLRMPFVNLGLCPEFGSSLILPRLLGQAKASELLLLG EGFTGEQAAQWGIATEALGSGEAALTKAREMALRFDELPAEAVRISKQLMRAPDRELIRKVIEEEGALFTQRLRSPEALA ALTGFIKRH
Sequences:
>Translated_249_residues MPEAILLHRERGLLTLRLNRPDKKNALTRAMYSQLADALKQANHDPEINAVLITGSAECFTAGNDIADFIQQPPSDLDSP VFHFMLNLLECRKPVIAAVAGAAVGIGTTLLLHCDLVYVARDARLRMPFVNLGLCPEFGSSLILPRLLGQAKASELLLLG EGFTGEQAAQWGIATEALGSGEAALTKAREMALRFDELPAEAVRISKQLMRAPDRELIRKVIEEEGALFTQRLRSPEALA ALTGFIKRH >Mature_248_residues PEAILLHRERGLLTLRLNRPDKKNALTRAMYSQLADALKQANHDPEINAVLITGSAECFTAGNDIADFIQQPPSDLDSPV FHFMLNLLECRKPVIAAVAGAAVGIGTTLLLHCDLVYVARDARLRMPFVNLGLCPEFGSSLILPRLLGQAKASELLLLGE GFTGEQAAQWGIATEALGSGEAALTKAREMALRFDELPAEAVRISKQLMRAPDRELIRKVIEEEGALFTQRLRSPEALAA LTGFIKRH
Specific function: Could possibly oxidize fatty acids using specific components [H]
COG id: NA
COG function: NA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]
Homologues:
Organism=Homo sapiens, GI260274832, Length=256, Percent_Identity=34.765625, Blast_Score=145, Evalue=4e-35, Organism=Homo sapiens, GI45643119, Length=256, Percent_Identity=34.765625, Blast_Score=145, Evalue=4e-35, Organism=Homo sapiens, GI260275230, Length=256, Percent_Identity=34.765625, Blast_Score=145, Evalue=4e-35, Organism=Homo sapiens, GI70995211, Length=187, Percent_Identity=33.1550802139037, Blast_Score=101, Evalue=6e-22, Organism=Homo sapiens, GI194097323, Length=236, Percent_Identity=28.3898305084746, Blast_Score=98, Evalue=7e-21, Organism=Homo sapiens, GI4757968, Length=204, Percent_Identity=26.4705882352941, Blast_Score=96, Evalue=3e-20, Organism=Homo sapiens, GI49355787, Length=204, Percent_Identity=26.4705882352941, Blast_Score=96, Evalue=3e-20, Organism=Homo sapiens, GI25453481, Length=204, Percent_Identity=25.9803921568627, Blast_Score=94, Evalue=1e-19, Organism=Homo sapiens, GI51538573, Length=204, Percent_Identity=25.9803921568627, Blast_Score=94, Evalue=1e-19, Organism=Homo sapiens, GI4757966, Length=202, Percent_Identity=26.2376237623762, Blast_Score=94, Evalue=1e-19, Organism=Homo sapiens, GI51538581, Length=202, Percent_Identity=26.2376237623762, Blast_Score=94, Evalue=1e-19, Organism=Homo sapiens, GI221136756, Length=256, Percent_Identity=23.828125, Blast_Score=90, Evalue=2e-18, Organism=Homo sapiens, GI221136753, Length=256, Percent_Identity=23.828125, Blast_Score=90, Evalue=2e-18, Organism=Homo sapiens, GI221307494, Length=255, Percent_Identity=23.921568627451, Blast_Score=89, Evalue=4e-18, Organism=Homo sapiens, GI116235487, Length=254, Percent_Identity=25.1968503937008, Blast_Score=88, Evalue=8e-18, Organism=Homo sapiens, GI37594469, Length=199, Percent_Identity=31.6582914572864, Blast_Score=82, Evalue=6e-16, Organism=Homo sapiens, GI37594471, Length=199, Percent_Identity=31.6582914572864, Blast_Score=81, Evalue=7e-16, Organism=Homo sapiens, GI68989263, Length=241, Percent_Identity=29.8755186721992, Blast_Score=79, Evalue=5e-15, Organism=Homo sapiens, GI20127408, Length=222, Percent_Identity=26.5765765765766, Blast_Score=70, Evalue=2e-12, Organism=Homo sapiens, GI31542718, Length=177, Percent_Identity=27.1186440677966, Blast_Score=69, Evalue=5e-12, Organism=Homo sapiens, GI157694516, Length=243, Percent_Identity=23.8683127572016, Blast_Score=67, Evalue=2e-11, Organism=Homo sapiens, GI213417737, Length=246, Percent_Identity=24.390243902439, Blast_Score=67, Evalue=2e-11, Organism=Homo sapiens, GI4502327, Length=252, Percent_Identity=25.7936507936508, Blast_Score=64, Evalue=9e-11, Organism=Escherichia coli, GI1787660, Length=257, Percent_Identity=35.0194552529183, Blast_Score=114, Evalue=8e-27, Organism=Escherichia coli, GI1787659, Length=249, Percent_Identity=32.1285140562249, Blast_Score=113, Evalue=1e-26, Organism=Escherichia coli, GI221142681, Length=214, Percent_Identity=30.8411214953271, Blast_Score=96, Evalue=3e-21, Organism=Escherichia coli, GI1790281, Length=170, Percent_Identity=30.5882352941176, Blast_Score=74, Evalue=1e-14, Organism=Escherichia coli, GI1788597, Length=173, Percent_Identity=27.7456647398844, Blast_Score=72, Evalue=4e-14, Organism=Caenorhabditis elegans, GI17535521, Length=256, Percent_Identity=30.078125, Blast_Score=124, Evalue=6e-29, Organism=Caenorhabditis elegans, GI17549921, Length=211, Percent_Identity=34.1232227488152, Blast_Score=110, Evalue=6e-25, Organism=Caenorhabditis elegans, GI17554946, Length=245, Percent_Identity=29.7959183673469, Blast_Score=104, Evalue=4e-23, Organism=Caenorhabditis elegans, GI25145438, Length=239, Percent_Identity=27.6150627615063, Blast_Score=93, Evalue=1e-19, Organism=Caenorhabditis elegans, GI17540714, Length=195, Percent_Identity=29.2307692307692, Blast_Score=85, Evalue=4e-17, Organism=Caenorhabditis elegans, GI17534483, Length=195, Percent_Identity=26.1538461538462, Blast_Score=77, Evalue=1e-14, Organism=Caenorhabditis elegans, GI17560910, Length=192, Percent_Identity=27.6041666666667, Blast_Score=76, Evalue=2e-14, Organism=Caenorhabditis elegans, GI17536985, Length=216, Percent_Identity=23.6111111111111, Blast_Score=74, Evalue=8e-14, Organism=Caenorhabditis elegans, GI17540306, Length=181, Percent_Identity=29.2817679558011, Blast_Score=67, Evalue=7e-12, Organism=Saccharomyces cerevisiae, GI6323314, Length=192, Percent_Identity=26.0416666666667, Blast_Score=67, Evalue=2e-12, Organism=Saccharomyces cerevisiae, GI6320241, Length=203, Percent_Identity=26.1083743842365, Blast_Score=65, Evalue=1e-11, Organism=Drosophila melanogaster, GI24654903, Length=255, Percent_Identity=32.156862745098, Blast_Score=137, Evalue=6e-33, Organism=Drosophila melanogaster, GI20129971, Length=199, Percent_Identity=30.6532663316583, Blast_Score=101, Evalue=4e-22, Organism=Drosophila melanogaster, GI24653477, Length=199, Percent_Identity=30.6532663316583, Blast_Score=101, Evalue=4e-22, Organism=Drosophila melanogaster, GI19920382, Length=223, Percent_Identity=27.3542600896861, Blast_Score=82, Evalue=4e-16, Organism=Drosophila melanogaster, GI24653139, Length=191, Percent_Identity=29.3193717277487, Blast_Score=76, Evalue=2e-14, Organism=Drosophila melanogaster, GI19922422, Length=183, Percent_Identity=24.5901639344262, Blast_Score=71, Evalue=6e-13, Organism=Drosophila melanogaster, GI21357171, Length=214, Percent_Identity=23.8317757009346, Blast_Score=64, Evalue=9e-11,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001753 - InterPro: IPR018376 [H]
Pfam domain/function: PF00378 ECH [H]
EC number: =4.2.1.17 [H]
Molecular weight: Translated: 27188; Mature: 27057
Theoretical pI: Translated: 6.67; Mature: 6.67
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.6 %Cys (Translated Protein) 2.4 %Met (Translated Protein) 4.0 %Cys+Met (Translated Protein) 1.6 %Cys (Mature Protein) 2.0 %Met (Mature Protein) 3.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MPEAILLHRERGLLTLRLNRPDKKNALTRAMYSQLADALKQANHDPEINAVLITGSAECF CCCCEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEE TAGNDIADFIQQPPSDLDSPVFHFMLNLLECRKPVIAAVAGAAVGIGTTLLLHCDLVYVA ECCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE RDARLRMPFVNLGLCPEFGSSLILPRLLGQAKASELLLLGEGFTGEQAAQWGIATEALGS CCCEEECCEECCCCCHHHCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHCCHHHHCCC GEAALTKAREMALRFDELPAEAVRISKQLMRAPDRELIRKVIEEEGALFTQRLRSPEALA CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHH ALTGFIKRH HHHHHHHCC >Mature Secondary Structure PEAILLHRERGLLTLRLNRPDKKNALTRAMYSQLADALKQANHDPEINAVLITGSAECF CCCEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEE TAGNDIADFIQQPPSDLDSPVFHFMLNLLECRKPVIAAVAGAAVGIGTTLLLHCDLVYVA ECCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE RDARLRMPFVNLGLCPEFGSSLILPRLLGQAKASELLLLGEGFTGEQAAQWGIATEALGS CCCEEECCEECCCCCHHHCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHCCHHHHCCC GEAALTKAREMALRFDELPAEAVRISKQLMRAPDRELIRKVIEEEGALFTQRLRSPEALA CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHH ALTGFIKRH HHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 12788972 [H]