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The map label for this gene is aspS [H]

Identifier: 77460632

GI number: 77460632

Start: 4970037

End: 4971812

Strand: Reverse

Name: aspS [H]

Synonym: Pfl01_4411

Alternate gene names: 77460632

Gene position: 4971812-4970037 (Counterclockwise)

Preceding gene: 77460633

Following gene: 77460631

Centisome position: 77.22

GC content: 61.04

Gene sequence:

>1776_bases
ATGATGCGCAGCCATTATTGCGGCCAACTGAACGAAAGCCTGGAAGGCCAGGAAATTACCCTTTGCGGATGGGTTCACCG
TCGTCGCGACCACGGCGGGGTGATTTTCCTCGATATCCGTGATCGTGACGGTCTGGCCCAGGTAGTGTTCGATCCGGATC
GCGCCGAGAGCTTCGCCGCCGCCGATCGCGTGCGCAGCGAATACGTCGTGAAGATCACCGGCAAGGTGCGTCTGCGTCCG
GCCGGTGCCACCAACGCCAATATGGCGTCGGGCATGATCGAAGTGCTGGGCTACGAACTGGAAGTGCTGAACGAGTCGGA
AACCCCGCCGTTCCCGCTGAACGAGTTCTCCGACGTCGGCGAAGAAACCCGCCTGCGTTATCGCTTCCTGGACCTGCGTC
GTCCGGAGATGGCCGAGAAGCTGCGTCTGCGCTCGCGTATGACCACCAGTATCCGTCGCTATCTGGACGAGAACGGCTTC
CTCGACGTCGAAACGCCGATCCTGACCCGTGCCACTCCGGAAGGCGCGCGCGACTACCTCGTACCGAGCCGTACCCACGC
CGGCTCGTTCTTCGCGCTGCCGCAATCGCCACAGCTGTTCAAGCAACTGCTGATGGTCGCCGGCTTCGACCGTTACTACC
AGATCGCCAAGTGCTTCCGCGACGAAGACCTGCGTGCCGACCGCCAGCCTGAGTTCACTCAGATCGACATCGAGACCAGC
TTCCTCGACGAAAAAGACATCATGGGCCTGACCGAAGGCATGATCCGCAACCTGTTCAAGGAAGTGCTGGATCTGGAATT
CGGCGAATTCCCGCACATGACATTCGAAGAAGCCATGCGCCGTTACGGTTCCGACAAGCCGGATCTGCGTAACCCGCTGG
AGCTGGTCGACGTGGCCGATCAGCTGAAAGAAGTGGATTTCAAGGTCTTCAGCGGCCCTGCCAACGATCCGAAATGCCGT
ATCGCCGCACTGCGCGTTCCAGGCGGGGCGAGCATGCCGCGCAAGCAGATCGACGACTACACCAAGTTCGTCGGCATCTA
CGGTGCCAAGGGTCTGGCGTACATCAAGGTCAACGAGCGTGCTGCCGGTGTTGAAGGTCTGCAATCGCCGATCGTGAAAA
ACATCCCGGAAGCCAACCTGAACGTGATCCTCGACCGCGTTGGCGCAGTCGACGGCGACATCGTGTTCTTCGGCGCCGAC
AAGGCCAAGATCGTCAGCGAAGCCCTGGGCGCGCTGCGTATCAAGCTCGGTCACGACCTGAAGCTGCTGACCTGCGAATG
GGCTCCGATGTGGGTTGTCGACTTCCCGATGTTCGAAGAGAACGACGACGGCAGCTTCTCCGCGCTGCACCACCCGTTCA
CCGCACCGAAGTGCTCGCCGGAAGAGCTGGAAGCCAACCCGGCCGGCGCTCTGTCCCGTGCCTACGACATGGTGCTGAAC
GGCACCGAACTGGGTGGCGGTTCGATCCGTATCCACCGTAAAGAGATGCAACAGGCGGTGTTCCGTCTGCTGGGCATCAA
CGAAGCGGAACAGGAAGAGAAGTTCGGCTTCCTGCTCGACGCCCTGAAATACGGCGCACCGCCACACGGTGGTCTGGCCT
TCGGTCTGGACCGTCTGGTGATGCTGATGACTGGCGCCCAGTCGATCCGTGAAGTGATCGCCTTCCCGAAAACCCAGAGT
GCTGCCGACGTGATGACGCAGGCGCCGGGTGTCGTGGACGCGAAGGCATTGCGCGAACTGCACATTCGTTTGCGCGAAAC
GCCGAAGGCTGAGTAA

Upstream 100 bases:

>100_bases
CCCAGGTCGAACGACACGCGCGAGTTTCCACAGGTTGCACAGTGCTTGAGTGCAGCGGGATCTCCACCGAATTTCGAATT
ACGAGAAGCGAACCACTACC

Downstream 100 bases:

>100_bases
GACGGGCCTGAAGGCGCATCTTCGGATGCGCCTTTTTTCTTTCTGCAGTGATTGAAAACAAGATTTTTTTGCTGGCCGAT
GCACCTGCATGGCGGGCATT

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 591; Mature: 591

Protein sequence:

>591_residues
MMRSHYCGQLNESLEGQEITLCGWVHRRRDHGGVIFLDIRDRDGLAQVVFDPDRAESFAAADRVRSEYVVKITGKVRLRP
AGATNANMASGMIEVLGYELEVLNESETPPFPLNEFSDVGEETRLRYRFLDLRRPEMAEKLRLRSRMTTSIRRYLDENGF
LDVETPILTRATPEGARDYLVPSRTHAGSFFALPQSPQLFKQLLMVAGFDRYYQIAKCFRDEDLRADRQPEFTQIDIETS
FLDEKDIMGLTEGMIRNLFKEVLDLEFGEFPHMTFEEAMRRYGSDKPDLRNPLELVDVADQLKEVDFKVFSGPANDPKCR
IAALRVPGGASMPRKQIDDYTKFVGIYGAKGLAYIKVNERAAGVEGLQSPIVKNIPEANLNVILDRVGAVDGDIVFFGAD
KAKIVSEALGALRIKLGHDLKLLTCEWAPMWVVDFPMFEENDDGSFSALHHPFTAPKCSPEELEANPAGALSRAYDMVLN
GTELGGGSIRIHRKEMQQAVFRLLGINEAEQEEKFGFLLDALKYGAPPHGGLAFGLDRLVMLMTGAQSIREVIAFPKTQS
AADVMTQAPGVVDAKALRELHIRLRETPKAE

Sequences:

>Translated_591_residues
MMRSHYCGQLNESLEGQEITLCGWVHRRRDHGGVIFLDIRDRDGLAQVVFDPDRAESFAAADRVRSEYVVKITGKVRLRP
AGATNANMASGMIEVLGYELEVLNESETPPFPLNEFSDVGEETRLRYRFLDLRRPEMAEKLRLRSRMTTSIRRYLDENGF
LDVETPILTRATPEGARDYLVPSRTHAGSFFALPQSPQLFKQLLMVAGFDRYYQIAKCFRDEDLRADRQPEFTQIDIETS
FLDEKDIMGLTEGMIRNLFKEVLDLEFGEFPHMTFEEAMRRYGSDKPDLRNPLELVDVADQLKEVDFKVFSGPANDPKCR
IAALRVPGGASMPRKQIDDYTKFVGIYGAKGLAYIKVNERAAGVEGLQSPIVKNIPEANLNVILDRVGAVDGDIVFFGAD
KAKIVSEALGALRIKLGHDLKLLTCEWAPMWVVDFPMFEENDDGSFSALHHPFTAPKCSPEELEANPAGALSRAYDMVLN
GTELGGGSIRIHRKEMQQAVFRLLGINEAEQEEKFGFLLDALKYGAPPHGGLAFGLDRLVMLMTGAQSIREVIAFPKTQS
AADVMTQAPGVVDAKALRELHIRLRETPKAE
>Mature_591_residues
MMRSHYCGQLNESLEGQEITLCGWVHRRRDHGGVIFLDIRDRDGLAQVVFDPDRAESFAAADRVRSEYVVKITGKVRLRP
AGATNANMASGMIEVLGYELEVLNESETPPFPLNEFSDVGEETRLRYRFLDLRRPEMAEKLRLRSRMTTSIRRYLDENGF
LDVETPILTRATPEGARDYLVPSRTHAGSFFALPQSPQLFKQLLMVAGFDRYYQIAKCFRDEDLRADRQPEFTQIDIETS
FLDEKDIMGLTEGMIRNLFKEVLDLEFGEFPHMTFEEAMRRYGSDKPDLRNPLELVDVADQLKEVDFKVFSGPANDPKCR
IAALRVPGGASMPRKQIDDYTKFVGIYGAKGLAYIKVNERAAGVEGLQSPIVKNIPEANLNVILDRVGAVDGDIVFFGAD
KAKIVSEALGALRIKLGHDLKLLTCEWAPMWVVDFPMFEENDDGSFSALHHPFTAPKCSPEELEANPAGALSRAYDMVLN
GTELGGGSIRIHRKEMQQAVFRLLGINEAEQEEKFGFLLDALKYGAPPHGGLAFGLDRLVMLMTGAQSIREVIAFPKTQS
AADVMTQAPGVVDAKALRELHIRLRETPKAE

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=599, Percent_Identity=40.567612687813, Blast_Score=426, Evalue=1e-119,
Organism=Homo sapiens, GI5031815, Length=249, Percent_Identity=27.710843373494, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI194272210, Length=249, Percent_Identity=27.710843373494, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI4758762, Length=265, Percent_Identity=27.5471698113208, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI45439306, Length=238, Percent_Identity=28.1512605042017, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI226491502, Length=260, Percent_Identity=25.7692307692308, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1788173, Length=585, Percent_Identity=61.8803418803419, Blast_Score=773, Evalue=0.0,
Organism=Escherichia coli, GI1789256, Length=310, Percent_Identity=27.741935483871, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1790571, Length=317, Percent_Identity=27.4447949526814, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1787161, Length=270, Percent_Identity=26.6666666666667, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI87082379, Length=309, Percent_Identity=26.8608414239482, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI32566633, Length=601, Percent_Identity=35.4409317803661, Blast_Score=335, Evalue=6e-92,
Organism=Caenorhabditis elegans, GI71994340, Length=250, Percent_Identity=26.8, Blast_Score=102, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17535927, Length=250, Percent_Identity=26.8, Blast_Score=102, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17535925, Length=250, Percent_Identity=26.8, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17551876, Length=277, Percent_Identity=23.4657039711191, Blast_Score=77, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6325153, Length=643, Percent_Identity=32.5038880248834, Blast_Score=285, Evalue=2e-77,
Organism=Saccharomyces cerevisiae, GI6320242, Length=262, Percent_Identity=26.7175572519084, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6321807, Length=253, Percent_Identity=28.4584980237154, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323011, Length=247, Percent_Identity=27.9352226720648, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324256, Length=172, Percent_Identity=23.2558139534884, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24584738, Length=610, Percent_Identity=36.8852459016393, Blast_Score=348, Evalue=9e-96,
Organism=Drosophila melanogaster, GI24640849, Length=257, Percent_Identity=28.0155642023346, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24640851, Length=257, Percent_Identity=28.0155642023346, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI17136276, Length=264, Percent_Identity=27.2727272727273, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19921528, Length=226, Percent_Identity=26.9911504424779, Blast_Score=67, Evalue=5e-11,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 66254; Mature: 66254

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRSHYCGQLNESLEGQEITLCGWVHRRRDHGGVIFLDIRDRDGLAQVVFDPDRAESFAA
CCCCCCCCHHHCCCCCCEEEEEEHHHHHCCCCCEEEEEEECCCCCEEEEECCCHHHHHHH
ADRVRSEYVVKITGKVRLRPAGATNANMASGMIEVLGYELEVLNESETPPFPLNEFSDVG
HHHHCCCEEEEEECEEEEEECCCCCCHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHCC
EETRLRYRFLDLRRPEMAEKLRLRSRMTTSIRRYLDENGFLDVETPILTRATPEGARDYL
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEECCCCCCCCCCC
VPSRTHAGSFFALPQSPQLFKQLLMVAGFDRYYQIAKCFRDEDLRADRQPEFTQIDIETS
CCCCCCCCCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHH
FLDEKDIMGLTEGMIRNLFKEVLDLEFGEFPHMTFEEAMRRYGSDKPDLRNPLELVDVAD
HCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHH
QLKEVDFKVFSGPANDPKCRIAALRVPGGASMPRKQIDDYTKFVGIYGAKGLAYIKVNER
HHHHCCEEEECCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCC
AAGVEGLQSPIVKNIPEANLNVILDRVGAVDGDIVFFGADKAKIVSEALGALRIKLGHDL
CCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHEEEEECCCE
KLLTCEWAPMWVVDFPMFEENDDGSFSALHHPFTAPKCSPEELEANPAGALSRAYDMVLN
EEEEECCCCEEEEECCCCCCCCCCCCHHHCCCCCCCCCCHHHCCCCCCHHHHHHHHHHCC
GTELGGGSIRIHRKEMQQAVFRLLGINEAEQEEKFGFLLDALKYGAPPHGGLAFGLDRLV
CCCCCCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
MLMTGAQSIREVIAFPKTQSAADVMTQAPGVVDAKALRELHIRLRETPKAE
HHHHCHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MMRSHYCGQLNESLEGQEITLCGWVHRRRDHGGVIFLDIRDRDGLAQVVFDPDRAESFAA
CCCCCCCCHHHCCCCCCEEEEEEHHHHHCCCCCEEEEEEECCCCCEEEEECCCHHHHHHH
ADRVRSEYVVKITGKVRLRPAGATNANMASGMIEVLGYELEVLNESETPPFPLNEFSDVG
HHHHCCCEEEEEECEEEEEECCCCCCHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHCC
EETRLRYRFLDLRRPEMAEKLRLRSRMTTSIRRYLDENGFLDVETPILTRATPEGARDYL
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEECCCCCCCCCCC
VPSRTHAGSFFALPQSPQLFKQLLMVAGFDRYYQIAKCFRDEDLRADRQPEFTQIDIETS
CCCCCCCCCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHH
FLDEKDIMGLTEGMIRNLFKEVLDLEFGEFPHMTFEEAMRRYGSDKPDLRNPLELVDVAD
HCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHH
QLKEVDFKVFSGPANDPKCRIAALRVPGGASMPRKQIDDYTKFVGIYGAKGLAYIKVNER
HHHHCCEEEECCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCC
AAGVEGLQSPIVKNIPEANLNVILDRVGAVDGDIVFFGADKAKIVSEALGALRIKLGHDL
CCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHEEEEECCCE
KLLTCEWAPMWVVDFPMFEENDDGSFSALHHPFTAPKCSPEELEANPAGALSRAYDMVLN
EEEEECCCCEEEEECCCCCCCCCCCCHHHCCCCCCCCCCHHHCCCCCCHHHHHHHHHHCC
GTELGGGSIRIHRKEMQQAVFRLLGINEAEQEEKFGFLLDALKYGAPPHGGLAFGLDRLV
CCCCCCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
MLMTGAQSIREVIAFPKTQSAADVMTQAPGVVDAKALRELHIRLRETPKAE
HHHHCHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA