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The map label for this gene is yfkH [H]

Identifier: 77460571

GI number: 77460571

Start: 4903123

End: 4904073

Strand: Reverse

Name: yfkH [H]

Synonym: Pfl01_4350

Alternate gene names: 77460571

Gene position: 4904073-4903123 (Counterclockwise)

Preceding gene: 77460572

Following gene: 77460570

Centisome position: 76.17

GC content: 59.41

Gene sequence:

>951_bases
ATGATTTTTCCCGACATGAAAGGTCTGCCCCTGCACCGGGTGATGGTGCGCACGATCACCGAATTCGTCGACGACGAGAT
GTCGACCTATGCCTCGGCATTGGCCTACCAGATGCTGTTCTCGCTGTTCCCGTTCATTCTGTTCCTGATTGCCCTGATCG
GTTTCCTGCACCTGCCGGACTTCTTCTCCTGGCTGCGCCTGCAATCGGAACTGGTGTTGCCGCCCCAGGCGCTGGAGCAG
GTCAACCCGGTGATCGATCAACTGCAGCAATCCAAGGGCGGCCTGCTTTCGGTGGGTATCGTCATCGCCCTGTACACCGC
GTCTGCCGGCGTGCGCCTGATGATGAGCGCGATGAACGCCGCCTACGATGTGGTCGAGGGCCGGCCTCTGTGGAAGCGTT
TTCCGCTGTCTGTCATCTACACCGTGGGCATCGCCGGCATGTTGCTGGTGGCCGCTGCGCTGATGGTGCTCGGCCCGCAG
GTGATGGGCTGGATCGCCGCGCAGGTCGGGTTGGAAGAAGTCATCGTTACCTTGTGGACCATCGCCCGCTGGCCGGTGAT
CGTGATCCTGATGATGGTGGCCGTGGCGCTGATCTACTACGTGATGCCCGACGTCAAACAGGAATTCCGCTTCATCACCC
CCGGCTCGGTGTTGGCGGTGGTGGTGTGGATCATCGCGTCCCTGGGCTTCGCGTTTTACGTCAAGACGTTCGCCAACTAC
AACGCCATGTATGGCAGCATCGGTGCGATCATCGTGCTGTTGCTGTATTTCTATATTTCCTCCGCCGTGTTGTTGCTCGG
CGCGGAGATGAATGCGGTCATCGAACATATGTCCAGCGAGGGCAAGGATTCGGGCGAGAAAGTCCCCGGCGAACTCGATG
AACATCCCAAACAACACGTTTCCGGCCTCGGGCGCGATCATTCGCTCAAGCCGAACACTGACGAAGTCTGA

Upstream 100 bases:

>100_bases
CACAGTGTTTCAAAGTCGCCAAGTAAGCTACTTTGATGAGAGAAAAACGGCCCCTGAGTCGCCACGACTTCAACCTGTTT
TGCGGCGAAAGGAGACGCGA

Downstream 100 bases:

>100_bases
CCATGATCCGTGAAATCCTGAAGATGGGCGACGAACGCCTGCTGCGCATCGCCCCGCCAGTGCCCGCTGAAATGCTCGAC
AGTCCCGAGCTGTGGCAACT

Product: ribonuclease BN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MIFPDMKGLPLHRVMVRTITEFVDDEMSTYASALAYQMLFSLFPFILFLIALIGFLHLPDFFSWLRLQSELVLPPQALEQ
VNPVIDQLQQSKGGLLSVGIVIALYTASAGVRLMMSAMNAAYDVVEGRPLWKRFPLSVIYTVGIAGMLLVAAALMVLGPQ
VMGWIAAQVGLEEVIVTLWTIARWPVIVILMMVAVALIYYVMPDVKQEFRFITPGSVLAVVVWIIASLGFAFYVKTFANY
NAMYGSIGAIIVLLLYFYISSAVLLLGAEMNAVIEHMSSEGKDSGEKVPGELDEHPKQHVSGLGRDHSLKPNTDEV

Sequences:

>Translated_316_residues
MIFPDMKGLPLHRVMVRTITEFVDDEMSTYASALAYQMLFSLFPFILFLIALIGFLHLPDFFSWLRLQSELVLPPQALEQ
VNPVIDQLQQSKGGLLSVGIVIALYTASAGVRLMMSAMNAAYDVVEGRPLWKRFPLSVIYTVGIAGMLLVAAALMVLGPQ
VMGWIAAQVGLEEVIVTLWTIARWPVIVILMMVAVALIYYVMPDVKQEFRFITPGSVLAVVVWIIASLGFAFYVKTFANY
NAMYGSIGAIIVLLLYFYISSAVLLLGAEMNAVIEHMSSEGKDSGEKVPGELDEHPKQHVSGLGRDHSLKPNTDEV
>Mature_316_residues
MIFPDMKGLPLHRVMVRTITEFVDDEMSTYASALAYQMLFSLFPFILFLIALIGFLHLPDFFSWLRLQSELVLPPQALEQ
VNPVIDQLQQSKGGLLSVGIVIALYTASAGVRLMMSAMNAAYDVVEGRPLWKRFPLSVIYTVGIAGMLLVAAALMVLGPQ
VMGWIAAQVGLEEVIVTLWTIARWPVIVILMMVAVALIYYVMPDVKQEFRFITPGSVLAVVVWIIASLGFAFYVKTFANY
NAMYGSIGAIIVLLLYFYISSAVLLLGAEMNAVIEHMSSEGKDSGEKVPGELDEHPKQHVSGLGRDHSLKPNTDEV

Specific function: 3' To 5' Exoribonuclease Necessary For 3'Maturation Of tRNA Molecules. [C]

COG id: COG1295

COG function: function code S; Predicted membrane protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790319, Length=287, Percent_Identity=24.390243902439, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017039
- InterPro:   IPR004664 [H]

Pfam domain/function: PF03631 Ribonuclease_BN [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 34905; Mature: 34905

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIFPDMKGLPLHRVMVRTITEFVDDEMSTYASALAYQMLFSLFPFILFLIALIGFLHLPD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FFSWLRLQSELVLPPQALEQVNPVIDQLQQSKGGLLSVGIVIALYTASAGVRLMMSAMNA
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AYDVVEGRPLWKRFPLSVIYTVGIAGMLLVAAALMVLGPQVMGWIAAQVGLEEVIVTLWT
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IARWPVIVILMMVAVALIYYVMPDVKQEFRFITPGSVLAVVVWIIASLGFAFYVKTFANY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
NAMYGSIGAIIVLLLYFYISSAVLLLGAEMNAVIEHMSSEGKDSGEKVPGELDEHPKQHV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
SGLGRDHSLKPNTDEV
HCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]