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The map label for this gene is def [H]

Identifier: 77460570

GI number: 77460570

Start: 4902581

End: 4903120

Strand: Reverse

Name: def [H]

Synonym: Pfl01_4349

Alternate gene names: 77460570

Gene position: 4903120-4902581 (Counterclockwise)

Preceding gene: 77460571

Following gene: 77460568

Centisome position: 76.15

GC content: 61.3

Gene sequence:

>540_bases
ATGATCCGTGAAATCCTGAAGATGGGCGACGAACGCCTGCTGCGCATCGCCCCGCCAGTGCCCGCTGAAATGCTCGACAG
TCCCGAGCTGTGGCAACTGATCGACGACATGTTCCAGACCATGGAAAGTGTCGGCGGTGTCGGCCTGGCCGCGCCGCAGA
TCGGCGTCGACCTGCAATTGGTGATCTTCGGTTTCGAGCACAGCGAACGCTACCCGGACGCCGAAGCCGTGCCGCAGACG
ATCCTGATCAACCCGCTGATCACGCCGCTCAGCCCGCTGATGGAAGAGGGCTTCGAAGGTTGCCTTTCTGTGCCCGGCCT
GCGGGGCGCGGTGGATCGTTATCAACAGATCCGTTACGAAGGCGTCGATCCCAAGGGTGAGCCGATCGTGCGCGTGGCCT
CTGGCTTCCATGCGCGGGTAGTGCAGCACGAATGCGATCACCTGATCGGGCGCCTGTACCCATCGCGCATTACCGATTTC
AGCAAATTCGGTTTCACCGAAGTGATGTTTCCGGACCTCGATCCCAACGCCGACGACTGA

Upstream 100 bases:

>100_bases
GCGAGAAAGTCCCCGGCGAACTCGATGAACATCCCAAACAACACGTTTCCGGCCTCGGGCGCGATCATTCGCTCAAGCCG
AACACTGACGAAGTCTGACC

Downstream 100 bases:

>100_bases
CTAGTCCGTGCGCGGCGCCAGCTCCATGGCAATCATCGGCTTGCTCCGCGCATAGCGGCTCAAACGTTCGGCCATGGCCT
GGGGCAGGGCGGGGTCGAAG

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MIREILKMGDERLLRIAPPVPAEMLDSPELWQLIDDMFQTMESVGGVGLAAPQIGVDLQLVIFGFEHSERYPDAEAVPQT
ILINPLITPLSPLMEEGFEGCLSVPGLRGAVDRYQQIRYEGVDPKGEPIVRVASGFHARVVQHECDHLIGRLYPSRITDF
SKFGFTEVMFPDLDPNADD

Sequences:

>Translated_179_residues
MIREILKMGDERLLRIAPPVPAEMLDSPELWQLIDDMFQTMESVGGVGLAAPQIGVDLQLVIFGFEHSERYPDAEAVPQT
ILINPLITPLSPLMEEGFEGCLSVPGLRGAVDRYQQIRYEGVDPKGEPIVRVASGFHARVVQHECDHLIGRLYPSRITDF
SKFGFTEVMFPDLDPNADD
>Mature_179_residues
MIREILKMGDERLLRIAPPVPAEMLDSPELWQLIDDMFQTMESVGGVGLAAPQIGVDLQLVIFGFEHSERYPDAEAVPQT
ILINPLITPLSPLMEEGFEGCLSVPGLRGAVDRYQQIRYEGVDPKGEPIVRVASGFHARVVQHECDHLIGRLYPSRITDF
SKFGFTEVMFPDLDPNADD

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=180, Percent_Identity=33.8888888888889, Blast_Score=92, Evalue=2e-19,
Organism=Escherichia coli, GI1789682, Length=151, Percent_Identity=35.0993377483444, Blast_Score=88, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24645726, Length=169, Percent_Identity=36.094674556213, Blast_Score=100, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24645728, Length=171, Percent_Identity=33.9181286549708, Blast_Score=100, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19977; Mature: 19977

Theoretical pI: Translated: 4.23; Mature: 4.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIREILKMGDERLLRIAPPVPAEMLDSPELWQLIDDMFQTMESVGGVGLAAPQIGVDLQL
CHHHHHHCCCHHEEEECCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEE
VIFGFEHSERYPDAEAVPQTILINPLITPLSPLMEEGFEGCLSVPGLRGAVDRYQQIRYE
EEEECCCCCCCCCHHHCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
GVDPKGEPIVRVASGFHARVVQHECDHLIGRLYPSRITDFSKFGFTEVMFPDLDPNADD
CCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCC
>Mature Secondary Structure
MIREILKMGDERLLRIAPPVPAEMLDSPELWQLIDDMFQTMESVGGVGLAAPQIGVDLQL
CHHHHHHCCCHHEEEECCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEE
VIFGFEHSERYPDAEAVPQTILINPLITPLSPLMEEGFEGCLSVPGLRGAVDRYQQIRYE
EEEECCCCCCCCCHHHCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
GVDPKGEPIVRVASGFHARVVQHECDHLIGRLYPSRITDFSKFGFTEVMFPDLDPNADD
CCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]