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The map label for this gene is mcrA [H]

Identifier: 77460518

GI number: 77460518

Start: 4854847

End: 4855560

Strand: Direct

Name: mcrA [H]

Synonym: Pfl01_4297

Alternate gene names: 77460518

Gene position: 4854847-4855560 (Clockwise)

Preceding gene: 77460517

Following gene: 77460519

Centisome position: 75.4

GC content: 48.88

Gene sequence:

>714_bases
ATGGACACTTCGAAAGAAAAAACTGGCTGGAGTGATGAGGAGCTAGAAGCTTCTGTAGACGCTTATCTAAAAATGCTGGT
TCTTGAACAACAAAATCAGCCATTCAATAAAGCGGACGAGAATCGACTCTTGCGAAAAGGCCCGCTGAGCAAACGCAGTG
CCTCGTCAGTCGAGTACCGCATGCAGAACATCTCTGCTGTGATGGATTTAATGGGACTGCCGAGAATTACAGGCTATGTT
TCGGCGAAGAATCTCGGCTCCGGTGTCATTACGCGTATCAAAGAGATCCTGGCTAAAAAAGACATGGAAGCGATACGAAA
CAGCTCACGAAGAGGCACTCAGCTGACTCGATTCCGGCTGATCGAACCTTCCGCACCTGAATTGAATGATGCGCCCCCGG
GTGTACTCAACCCGAAGCAGACTAGCGCGACGGTAGTTAGGCTTTCGCGCAAATTAAATGTGAAAAAATGGGTTCTGAAA
CGTTCTCAAGGTATTTGCGAAGGGTGTGGCCAAAAAGCGCCATTCCAAGATGCTGATGGCAAACCTTTTCTTGAAGTTCA
TCATCTGAAACATCTCGTTAACGGGGGGACAGACCGTACGAGCAACGCAGTAGCCCTCTGCCCGAACTGCCATCGACGTT
GCCATTACTCGAGCGACAAAGAAGAGTTCACTGCTAAGTTTTATAGGAGCGTCGAGGGGTTAGAGGCTGAATAG

Upstream 100 bases:

>100_bases
GGACCTTGCAAGATCCGTCGATATTTTCTGTAGGAATTTTCGTAGACAGCTGTGGTGGCCACTCAGTATCGTCCGAGGGT
TTTCAACCCTCGGCGACTGT

Downstream 100 bases:

>100_bases
CTACAAGGTATTTGGCAGTCAGCCAGTTAGAACGCGTTCACCCAGTTGTCCGTTTTGGGGCAGCTTCACAACCCATCGGG
GATAATACCCCTCCCCCCAC

Product: HNH endonuclease

Products: NA

Alternate protein names: EcoKMcrA [H]

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MDTSKEKTGWSDEELEASVDAYLKMLVLEQQNQPFNKADENRLLRKGPLSKRSASSVEYRMQNISAVMDLMGLPRITGYV
SAKNLGSGVITRIKEILAKKDMEAIRNSSRRGTQLTRFRLIEPSAPELNDAPPGVLNPKQTSATVVRLSRKLNVKKWVLK
RSQGICEGCGQKAPFQDADGKPFLEVHHLKHLVNGGTDRTSNAVALCPNCHRRCHYSSDKEEFTAKFYRSVEGLEAE

Sequences:

>Translated_237_residues
MDTSKEKTGWSDEELEASVDAYLKMLVLEQQNQPFNKADENRLLRKGPLSKRSASSVEYRMQNISAVMDLMGLPRITGYV
SAKNLGSGVITRIKEILAKKDMEAIRNSSRRGTQLTRFRLIEPSAPELNDAPPGVLNPKQTSATVVRLSRKLNVKKWVLK
RSQGICEGCGQKAPFQDADGKPFLEVHHLKHLVNGGTDRTSNAVALCPNCHRRCHYSSDKEEFTAKFYRSVEGLEAE
>Mature_237_residues
MDTSKEKTGWSDEELEASVDAYLKMLVLEQQNQPFNKADENRLLRKGPLSKRSASSVEYRMQNISAVMDLMGLPRITGYV
SAKNLGSGVITRIKEILAKKDMEAIRNSSRRGTQLTRFRLIEPSAPELNDAPPGVLNPKQTSATVVRLSRKLNVKKWVLK
RSQGICEGCGQKAPFQDADGKPFLEVHHLKHLVNGGTDRTSNAVALCPNCHRRCHYSSDKEEFTAKFYRSVEGLEAE

Specific function: Restriction of 5-methyl and 5-hydroxymethylcytosines at the specific DNA sequence C(me)CGG [H]

COG id: COG1403

COG function: function code V; Restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787406, Length=82, Percent_Identity=52.4390243902439, Blast_Score=104, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002711
- InterPro:   IPR003615 [H]

Pfam domain/function: PF01844 HNH [H]

EC number: 3.1.21.- [C]

Molecular weight: Translated: 26547; Mature: 26547

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTSKEKTGWSDEELEASVDAYLKMLVLEQQNQPFNKADENRLLRKGPLSKRSASSVEYR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCHHHHHHHH
MQNISAVMDLMGLPRITGYVSAKNLGSGVITRIKEILAKKDMEAIRNSSRRGTQLTRFRL
HHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHEEEE
IEPSAPELNDAPPGVLNPKQTSATVVRLSRKLNVKKWVLKRSQGICEGCGQKAPFQDADG
ECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCHHHHCCCCCCCCCCCC
KPFLEVHHLKHLVNGGTDRTSNAVALCPNCHRRCHYSSDKEEFTAKFYRSVEGLEAE
CCHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHCCCCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MDTSKEKTGWSDEELEASVDAYLKMLVLEQQNQPFNKADENRLLRKGPLSKRSASSVEYR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCHHHHHHHH
MQNISAVMDLMGLPRITGYVSAKNLGSGVITRIKEILAKKDMEAIRNSSRRGTQLTRFRL
HHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHEEEE
IEPSAPELNDAPPGVLNPKQTSATVVRLSRKLNVKKWVLKRSQGICEGCGQKAPFQDADG
ECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCHHHHCCCCCCCCCCCC
KPFLEVHHLKHLVNGGTDRTSNAVALCPNCHRRCHYSSDKEEFTAKFYRSVEGLEAE
CCHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHCCCCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1938927; 8905232; 9278503 [H]