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The map label for this gene is astE

Identifier: 77460500

GI number: 77460500

Start: 4827686

End: 4828696

Strand: Reverse

Name: astE

Synonym: Pfl01_4279

Alternate gene names: 77460500

Gene position: 4828696-4827686 (Counterclockwise)

Preceding gene: 77460501

Following gene: 77460498

Centisome position: 75.0

GC content: 61.42

Gene sequence:

>1011_bases
ATGCTCGCCCTCGGCAAACTGCTTGAACTGACCCTCGCCGGCCGTGAACCGGCGGAGAAGACTCAACTGACTGTCGAAGG
CGTGCGCATGCGCTGGTTGAGCGAGGGTGCGCTGGAAGTCCGGCCACCCGAAGCGCGCGACAATGGCCTGGACCTGCTGC
TGTCTGCAGGCATCCACGGCAACGAAACGGCACCGATCGAATTGCTCGACCGGTTGCTGCATGACATCGCCCGCGGCGAT
CTGAAGCCGCGCGCACGCATTCTGTTCCTGTTCGGCAACCCGGAAGCGATTCGCAAGGGCGAGCGTTTCGTCGAGCAGGA
CGTCAATCGGCTGTTCAACGGCCGTCACGAACAAAGCAGCGGTTCCGAAGCCCTGCGTGCCTGCGAGCTGGAACGTCTGG
CGGCGAGTTTCTTCAGTCTTCCTGATCGTCAGCGCCTGCACTACGACCTGCACACCGCGATTCGCGGTTCGAAGATCGAG
CAGTTCGCCCTTTATCCGTGGAAGGAAGGTCGCCAGCATTCCCGTCTTGAGCTGGCCCGCCTGCGCGCCGCCGGCATGGA
GGCGGTGCTGTTGCAGAACAAGCCGTCGATCGTGTTCAGCTCTTACACCTACGACAAGCTGGGGGCCGAGTCCTTCACGC
TGGAACTGGGCAAGGCGCGGCCATTCGGGCAGAACGCCGGGGTCAATGTGTCGCTGTTGGAAACCCGCCTGAAGCAGATC
ATCGAAGGCACCGAACCAGAGATGGCCGAGCAAGGCCTGGATGGTCTGCAACTGTTCAGCGTGGCTCGGGAAATCATCAA
GCACAGCGATGCGTTCCGCCTGAACCTGCCGGCGGACATCGAAAACTTTTCCGAACTGGATGTGGGTTATGTGCTGGCCG
AAGACCTGGCCAATACCCGCTGGATCATCGAAGAGCAGGGCGCGCGGATCATCTTCCCCAACCCCAAGGTCAAGAACGGC
CTGCGGGCGGGTATTCTGATCGTGCCGACCACTGACGAAAATCTTGCCTGA

Upstream 100 bases:

>100_bases
GGTAAGTCTGCAACTGGAGATGGAACCGGCTGACCTCGGCGACGATGACGGCCACGTGCACGGCCCGGATTGCAATCACG
ACCACTAAGGAAACCGCTCT

Downstream 100 bases:

>100_bases
CACTGAAATCTCCCCCACGCAGCGCGCGGGGGAGATCGTTATTGCATCAGACCGCTACAGCACGCGGCTCGGTGCGGCGC
AATGCACGCACTTTCTGCAA

Product: succinylglutamate desuccinylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MLALGKLLELTLAGREPAEKTQLTVEGVRMRWLSEGALEVRPPEARDNGLDLLLSAGIHGNETAPIELLDRLLHDIARGD
LKPRARILFLFGNPEAIRKGERFVEQDVNRLFNGRHEQSSGSEALRACELERLAASFFSLPDRQRLHYDLHTAIRGSKIE
QFALYPWKEGRQHSRLELARLRAAGMEAVLLQNKPSIVFSSYTYDKLGAESFTLELGKARPFGQNAGVNVSLLETRLKQI
IEGTEPEMAEQGLDGLQLFSVAREIIKHSDAFRLNLPADIENFSELDVGYVLAEDLANTRWIIEEQGARIIFPNPKVKNG
LRAGILIVPTTDENLA

Sequences:

>Translated_336_residues
MLALGKLLELTLAGREPAEKTQLTVEGVRMRWLSEGALEVRPPEARDNGLDLLLSAGIHGNETAPIELLDRLLHDIARGD
LKPRARILFLFGNPEAIRKGERFVEQDVNRLFNGRHEQSSGSEALRACELERLAASFFSLPDRQRLHYDLHTAIRGSKIE
QFALYPWKEGRQHSRLELARLRAAGMEAVLLQNKPSIVFSSYTYDKLGAESFTLELGKARPFGQNAGVNVSLLETRLKQI
IEGTEPEMAEQGLDGLQLFSVAREIIKHSDAFRLNLPADIENFSELDVGYVLAEDLANTRWIIEEQGARIIFPNPKVKNG
LRAGILIVPTTDENLA
>Mature_336_residues
MLALGKLLELTLAGREPAEKTQLTVEGVRMRWLSEGALEVRPPEARDNGLDLLLSAGIHGNETAPIELLDRLLHDIARGD
LKPRARILFLFGNPEAIRKGERFVEQDVNRLFNGRHEQSSGSEALRACELERLAASFFSLPDRQRLHYDLHTAIRGSKIE
QFALYPWKEGRQHSRLELARLRAAGMEAVLLQNKPSIVFSSYTYDKLGAESFTLELGKARPFGQNAGVNVSLLETRLKQI
IEGTEPEMAEQGLDGLQLFSVAREIIKHSDAFRLNLPADIENFSELDVGYVLAEDLANTRWIIEEQGARIIFPNPKVKNG
LRAGILIVPTTDENLA

Specific function: Transforms N(2)-succinylglutamate into succinate and glutamate

COG id: COG2988

COG function: function code E; Succinylglutamate desuccinylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AspA/AstE family. Succinylglutamate desuccinylase subfamily

Homologues:

Organism=Escherichia coli, GI1788040, Length=326, Percent_Identity=37.1165644171779, Blast_Score=202, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ASTE_PSEPF (Q3K888)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_350007.1
- HSSP:   Q9KSL4
- ProteinModelPortal:   Q3K888
- SMR:   Q3K888
- STRING:   Q3K888
- GeneID:   3712891
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_4279
- eggNOG:   COG2988
- HOGENOM:   HBG298271
- OMA:   RWRWLGH
- ProtClustDB:   PRK05324
- BioCyc:   PFLU205922:PFL_4279-MONOMER
- HAMAP:   MF_00767
- InterPro:   IPR007036
- InterPro:   IPR016681
- PIRSF:   PIRSF017020
- TIGRFAMs:   TIGR03242

Pfam domain/function: PF04952 AstE_AspA

EC number: =3.5.1.96

Molecular weight: Translated: 37592; Mature: 37592

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: ACT_SITE 215-215

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLALGKLLELTLAGREPAEKTQLTVEGVRMRWLSEGALEVRPPEARDNGLDLLLSAGIHG
CCCHHHHHHHHCCCCCCCHHHEEEHHHHHHHHHCCCCEEECCCCCCCCCCEEEEECCCCC
NETAPIELLDRLLHDIARGDLKPRARILFLFGNPEAIRKGERFVEQDVNRLFNGRHEQSS
CCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCC
GSEALRACELERLAASFFSLPDRQRLHYDLHTAIRGSKIEQFALYPWKEGRQHSRLELAR
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHH
LRAAGMEAVLLQNKPSIVFSSYTYDKLGAESFTLELGKARPFGQNAGVNVSLLETRLKQI
HHHCCCEEEEECCCCCEEEECCCHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHH
IEGTEPEMAEQGLDGLQLFSVAREIIKHSDAFRLNLPADIENFSELDVGYVLAEDLANTR
HCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHCCHHHHHHHHHCCCE
WIIEEQGARIIFPNPKVKNGLRAGILIVPTTDENLA
EEEECCCCEEECCCCCCCCCCCCCEEEEECCCCCCC
>Mature Secondary Structure
MLALGKLLELTLAGREPAEKTQLTVEGVRMRWLSEGALEVRPPEARDNGLDLLLSAGIHG
CCCHHHHHHHHCCCCCCCHHHEEEHHHHHHHHHCCCCEEECCCCCCCCCCEEEEECCCCC
NETAPIELLDRLLHDIARGDLKPRARILFLFGNPEAIRKGERFVEQDVNRLFNGRHEQSS
CCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCC
GSEALRACELERLAASFFSLPDRQRLHYDLHTAIRGSKIEQFALYPWKEGRQHSRLELAR
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHH
LRAAGMEAVLLQNKPSIVFSSYTYDKLGAESFTLELGKARPFGQNAGVNVSLLETRLKQI
HHHCCCEEEEECCCCCEEEECCCHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHH
IEGTEPEMAEQGLDGLQLFSVAREIIKHSDAFRLNLPADIENFSELDVGYVLAEDLANTR
HCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHCCHHHHHHHHHCCCE
WIIEEQGARIIFPNPKVKNGLRAGILIVPTTDENLA
EEEECCCCEEECCCCCCCCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA