Click here to switch to the map view.

The map label for this gene is rhtC [H]

Identifier: 77460410

GI number: 77460410

Start: 4726253

End: 4726891

Strand: Direct

Name: rhtC [H]

Synonym: Pfl01_4189

Alternate gene names: 77460410

Gene position: 4726253-4726891 (Clockwise)

Preceding gene: 77460409

Following gene: 77460411

Centisome position: 73.41

GC content: 59.94

Gene sequence:

>639_bases
ATGACATCGAATTACCTGGGCGAGTTTCTGGCGCTGGCCACCATCCACTTTCTGGCCGTGGTCGCTCCCGGACCGGACTT
CGCCGTGACCATACGCCAGAGTGTGCGCTTCGGCCGCTTGGTCGGCATCTGCACGGCACTGGGCATCGGCGCGGGAATTT
CCGTGCACGTGCTGTACACCCTGCTGGGCGTCGGCGCCCTGATGCACACCACGCCCTGGTTGCTGACCGTCGCCAAAGTG
GTCGGCGGTGCTTACATTCTTTATCTGGGCGTCAGCCTGATCCGCAGCAAACCCAAGACGACAATGGAAGGCGAAAAGAC
CAGTGACGAACCACTGGTCGAGCAATCGCTGTTCAAGGCGTTTTCCACCGGTTTCCTGACCAACGCCACCAACCCCAAGG
CCACGCTGTTTTTCCTGGCGATCTTCACCACGATCATCAGCGCCAGCACGCCGCTGCAAATCCAGGCCCTGTACGGGTTG
TGGATGTGCGGGGTGAATGCGTTGTGGTTCGTGATCGTCGCGCTGTTCTTTTCCAGCAGCAAAGTGCGCGTGCTGTTCAT
GCGCATGGGCCACTGGTTCGAGCGCAGCATGGGGGTGATTCTGATCCTGTTTGCCGGGCGTCTTGTGCTGTCGATGTAA

Upstream 100 bases:

>100_bases
ATGCACTCGGTCACGCCGGGCGTGGCCGATCCGCGCTTGAGCCTTTCGTTTTTTGTCGGCTATCGCGGCAATGCTTCACC
CCTGACTTTCTGGAGCTGAA

Downstream 100 bases:

>100_bases
GCACATTGATCGGAGAAGCCCGGCCCAGACGGATTGGGCGGGCTTTTTCGATTCTGCGCCGCTTTTGCATTTCTGTGCGA
CGCGTCCCACTTCGGGGGCT

Product: lysine exporter protein LysE/YggA

Products: Proton [Cytoplasm]; L-threonine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MTSNYLGEFLALATIHFLAVVAPGPDFAVTIRQSVRFGRLVGICTALGIGAGISVHVLYTLLGVGALMHTTPWLLTVAKV
VGGAYILYLGVSLIRSKPKTTMEGEKTSDEPLVEQSLFKAFSTGFLTNATNPKATLFFLAIFTTIISASTPLQIQALYGL
WMCGVNALWFVIVALFFSSSKVRVLFMRMGHWFERSMGVILILFAGRLVLSM

Sequences:

>Translated_212_residues
MTSNYLGEFLALATIHFLAVVAPGPDFAVTIRQSVRFGRLVGICTALGIGAGISVHVLYTLLGVGALMHTTPWLLTVAKV
VGGAYILYLGVSLIRSKPKTTMEGEKTSDEPLVEQSLFKAFSTGFLTNATNPKATLFFLAIFTTIISASTPLQIQALYGL
WMCGVNALWFVIVALFFSSSKVRVLFMRMGHWFERSMGVILILFAGRLVLSM
>Mature_211_residues
TSNYLGEFLALATIHFLAVVAPGPDFAVTIRQSVRFGRLVGICTALGIGAGISVHVLYTLLGVGALMHTTPWLLTVAKVV
GGAYILYLGVSLIRSKPKTTMEGEKTSDEPLVEQSLFKAFSTGFLTNATNPKATLFFLAIFTTIISASTPLQIQALYGLW
MCGVNALWFVIVALFFSSSKVRVLFMRMGHWFERSMGVILILFAGRLVLSM

Specific function: Conducts the efflux of threonine [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994978, Length=205, Percent_Identity=28.2926829268293, Blast_Score=84, Evalue=5e-18,
Organism=Escherichia coli, GI1786522, Length=189, Percent_Identity=28.5714285714286, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788099, Length=132, Percent_Identity=31.8181818181818, Blast_Score=67, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 23061; Mature: 22930

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSNYLGEFLALATIHFLAVVAPGPDFAVTIRQSVRFGRLVGICTALGIGAGISVHVLYT
CCCHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
LLGVGALMHTTPWLLTVAKVVGGAYILYLGVSLIRSKPKTTMEGEKTSDEPLVEQSLFKA
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
FSTGFLTNATNPKATLFFLAIFTTIISASTPLQIQALYGLWMCGVNALWFVIVALFFSSS
HHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KVRVLFMRMGHWFERSMGVILILFAGRLVLSM
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSNYLGEFLALATIHFLAVVAPGPDFAVTIRQSVRFGRLVGICTALGIGAGISVHVLYT
CCHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
LLGVGALMHTTPWLLTVAKVVGGAYILYLGVSLIRSKPKTTMEGEKTSDEPLVEQSLFKA
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
FSTGFLTNATNPKATLFFLAIFTTIISASTPLQIQALYGLWMCGVNALWFVIVALFFSSS
HHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KVRVLFMRMGHWFERSMGVILILFAGRLVLSM
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; L-threonine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + L-threonine [Cytoplasm] = Proton [Cytoplasm] + L-threonine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]