Click here to switch to the map view.

The map label for this gene is tmk

Identifier: 77460373

GI number: 77460373

Start: 4689738

End: 4690370

Strand: Reverse

Name: tmk

Synonym: Pfl01_4152

Alternate gene names: 77460373

Gene position: 4690370-4689738 (Counterclockwise)

Preceding gene: 77460374

Following gene: 77460372

Centisome position: 72.85

GC content: 63.67

Gene sequence:

>633_bases
GTGACTGGCTTGTTTATTACCCTGGAAGGCCCGGAAGGCGCCGGCAAAAGCACCAACCGCGAATACCTCGCCGAGCGCCT
GCGCGCCGCCGGTATCGAAGTGGTGCTGACCCGCGAGCCCGGCGGTACACCGCTGGCCGAGCGCATTCGTGAGGTGTTGC
TGGCCCCGGTCGACGAAGTGATGAACCCGGACACCGAGCTGCTGCTGGTGTTCGCCGCCCGTGCCCAGCATCTGGCCGAA
GTCATTCGCCCGGCACTGGCCCGTGGTGCGGTGGTGCTGTGTGACCGTTTCACCGATTCGACTTATGCCTATCAGGGTGG
CGGTCGCGGTTTGTCGCTGGAGCGCATCGCGGCGCTTGAGACTTTCGTGCAGGGCGACCTGCGTCCCGACCTGACGCTGA
TTTTCGATCTGCCGGTGGAAATCGGCCTGGCCCGCGCAAGTGCCCGAGGTCGTCTGGATCGTTTCGAGCTGGAAGGCCGT
GAGTTTTTCGAGGCCGTGCGCAATGCGTTCCTCAAGCGTGCCGAAGACGATCCGGCGCGTTACGTGCGGATCGACGCCGG
TCAGCCGTTGGTCAAGGTTCAACAATCGCTGGATACCCTGATTCCGAATTTGCTGGAGCTGAGCCGTGGCTGA

Upstream 100 bases:

>100_bases
GAATCGTTGCCGCAGCCGCCCGCTCAAGCGCCTGCGCCGACGCCGGAACCGGAAGCCTCCGCTCCCCAAAACACGCAATG
ACTTTGATTAAGGACTGCCT

Downstream 100 bases:

>100_bases
GGCCTATCCGTGGCAGGACAGCCTCTGGCAGCAACTGGCGGGGCGCACTCAGCATGCCCACGCTTATCTGCTGCACGGCC
CGGCCGGGATCGGCAAGCGC

Product: thymidylate kinase

Products: NA

Alternate protein names: dTMP kinase

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MTGLFITLEGPEGAGKSTNREYLAERLRAAGIEVVLTREPGGTPLAERIREVLLAPVDEVMNPDTELLLVFAARAQHLAE
VIRPALARGAVVLCDRFTDSTYAYQGGGRGLSLERIAALETFVQGDLRPDLTLIFDLPVEIGLARASARGRLDRFELEGR
EFFEAVRNAFLKRAEDDPARYVRIDAGQPLVKVQQSLDTLIPNLLELSRG

Sequences:

>Translated_210_residues
MTGLFITLEGPEGAGKSTNREYLAERLRAAGIEVVLTREPGGTPLAERIREVLLAPVDEVMNPDTELLLVFAARAQHLAE
VIRPALARGAVVLCDRFTDSTYAYQGGGRGLSLERIAALETFVQGDLRPDLTLIFDLPVEIGLARASARGRLDRFELEGR
EFFEAVRNAFLKRAEDDPARYVRIDAGQPLVKVQQSLDTLIPNLLELSRG
>Mature_209_residues
TGLFITLEGPEGAGKSTNREYLAERLRAAGIEVVLTREPGGTPLAERIREVLLAPVDEVMNPDTELLLVFAARAQHLAEV
IRPALARGAVVLCDRFTDSTYAYQGGGRGLSLERIAALETFVQGDLRPDLTLIFDLPVEIGLARASARGRLDRFELEGRE
FFEAVRNAFLKRAEDDPARYVRIDAGQPLVKVQQSLDTLIPNLLELSRG

Specific function: Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis

COG id: COG0125

COG function: function code F; Thymidylate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate kinase family

Homologues:

Organism=Escherichia coli, GI1787340, Length=210, Percent_Identity=45.7142857142857, Blast_Score=166, Evalue=8e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KTHY_PSEPF (Q3K8L5)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_349880.1
- HSSP:   P65248
- ProteinModelPortal:   Q3K8L5
- SMR:   Q3K8L5
- STRING:   Q3K8L5
- GeneID:   3714917
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_4152
- eggNOG:   COG0125
- HOGENOM:   HBG626009
- OMA:   LFITLEG
- ProtClustDB:   PRK00698
- BioCyc:   PFLU205922:PFL_4152-MONOMER
- HAMAP:   MF_00165
- InterPro:   IPR018095
- InterPro:   IPR018094
- PANTHER:   PTHR10344
- TIGRFAMs:   TIGR00041

Pfam domain/function: PF02223 Thymidylate_kin

EC number: =2.7.4.9

Molecular weight: Translated: 23113; Mature: 22982

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS01331 THYMIDYLATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGLFITLEGPEGAGKSTNREYLAERLRAAGIEVVLTREPGGTPLAERIREVLLAPVDEV
CCEEEEEEECCCCCCCCCCHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHH
MNPDTELLLVFAARAQHLAEVIRPALARGAVVLCDRFTDSTYAYQGGGRGLSLERIAALE
HCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEECCCCCCCCHHHHHHHH
TFVQGDLRPDLTLIFDLPVEIGLARASARGRLDRFELEGREFFEAVRNAFLKRAEDDPAR
HHHHCCCCCCEEEEEECCHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCCE
YVRIDAGQPLVKVQQSLDTLIPNLLELSRG
EEEECCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TGLFITLEGPEGAGKSTNREYLAERLRAAGIEVVLTREPGGTPLAERIREVLLAPVDEV
CEEEEEEECCCCCCCCCCHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHH
MNPDTELLLVFAARAQHLAEVIRPALARGAVVLCDRFTDSTYAYQGGGRGLSLERIAALE
HCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEECCCCCCCCHHHHHHHH
TFVQGDLRPDLTLIFDLPVEIGLARASARGRLDRFELEGREFFEAVRNAFLKRAEDDPAR
HHHHCCCCCCEEEEEECCHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCCE
YVRIDAGQPLVKVQQSLDTLIPNLLELSRG
EEEECCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA