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The map label for this gene is holB [H]

Identifier: 77460372

GI number: 77460372

Start: 4688762

End: 4689745

Strand: Reverse

Name: holB [H]

Synonym: Pfl01_4151

Alternate gene names: 77460372

Gene position: 4689745-4688762 (Counterclockwise)

Preceding gene: 77460373

Following gene: 77460371

Centisome position: 72.84

GC content: 63.01

Gene sequence:

>984_bases
GTGGCTGAGGCCTATCCGTGGCAGGACAGCCTCTGGCAGCAACTGGCGGGGCGCACTCAGCATGCCCACGCTTATCTGCT
GCACGGCCCGGCCGGGATCGGCAAGCGCGCGCTGGCCGAGCGGCTGATGGCCAGCCTGCTGTGCCAGCGTCCGACGCCTG
AAGCCTGCGGCGAATGCAAATCCTGCCTGCTGCTCAAGGCCGGCAGCCATCCGGACAATTACATCCTCGAACCGGAAGAA
GCGGACAAGGCGATCAAGGTCGACCAGGTGCGCGATCTGGTCAGTTTCGTGGTGCAGACCGCGCAACTGGGCGGGCGCAA
GGTGGTGCTGATCGAGCCGGTGGAGTCGATGAACATCAACGCCGCCAACGCCTTGCTCAAGAGCCTTGAAGAGCCGTCCG
GCGATACCGTGCTGTTGCTGGTCAGCCACCAGCCGAGCCGGTTGTTGCCGACCATCAAGAGCCGCTGCGTGCAACAGGCC
TGTCCGCTGCCGAGCGAATCCGTGAGCCTGCAATGGCTGGCTCAGGCACTGCCGGAATGTTCCGAAGACGAACGTGTCGA
ACTGCTGACCCTGGCCGCCGGCTCGCCGTTGGCCGCCGTCACGTTGCAGGGGCAGGGCGTACGTGAACAGCGCGCGCAGG
TGGTGGAAGGCGTGAAGAAGCTGCTCAAGCAGCAGCAATCGCCGACGCAACTGGCCGAGGAGTGGAAAAACATTCCGATG
CTGCGGCTGTTCGACTGGTTCTGCGACTGGTCGAGCCTGATCCTGCGCTATCAATTGACCCAGGATGAAGCAGGCCTCGG
CCTGACCGACATGCGCAAGGTCGTGCAGTACCTGGCGCAGAAAAGTGCCCAGCCTAAAGTGCTGGATATTCAGGACTGGA
TCCTCGCCCAGCGTCAGATAGTCCTGAGCAAGGCCAACCTCAATGCGGCGTTGCTGCTGGAAGCGTTGCTGGTGCAATGG
GCGAGCTTGCCTGGCCAAAGATAA

Upstream 100 bases:

>100_bases
GACGATCCGGCGCGTTACGTGCGGATCGACGCCGGTCAGCCGTTGGTCAAGGTTCAACAATCGCTGGATACCCTGATTCC
GAATTTGCTGGAGCTGAGCC

Downstream 100 bases:

>100_bases
GAATGTGTACCTAGACTCTGAACATCAGTAGTGGAGGTCAACATGAATGAACCTGTCAGCCCGGGGCCGCGCAACGGCAT
CTTGTCCCTGACCATCAAGG

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MAEAYPWQDSLWQQLAGRTQHAHAYLLHGPAGIGKRALAERLMASLLCQRPTPEACGECKSCLLLKAGSHPDNYILEPEE
ADKAIKVDQVRDLVSFVVQTAQLGGRKVVLIEPVESMNINAANALLKSLEEPSGDTVLLLVSHQPSRLLPTIKSRCVQQA
CPLPSESVSLQWLAQALPECSEDERVELLTLAAGSPLAAVTLQGQGVREQRAQVVEGVKKLLKQQQSPTQLAEEWKNIPM
LRLFDWFCDWSSLILRYQLTQDEAGLGLTDMRKVVQYLAQKSAQPKVLDIQDWILAQRQIVLSKANLNAALLLEALLVQW
ASLPGQR

Sequences:

>Translated_327_residues
MAEAYPWQDSLWQQLAGRTQHAHAYLLHGPAGIGKRALAERLMASLLCQRPTPEACGECKSCLLLKAGSHPDNYILEPEE
ADKAIKVDQVRDLVSFVVQTAQLGGRKVVLIEPVESMNINAANALLKSLEEPSGDTVLLLVSHQPSRLLPTIKSRCVQQA
CPLPSESVSLQWLAQALPECSEDERVELLTLAAGSPLAAVTLQGQGVREQRAQVVEGVKKLLKQQQSPTQLAEEWKNIPM
LRLFDWFCDWSSLILRYQLTQDEAGLGLTDMRKVVQYLAQKSAQPKVLDIQDWILAQRQIVLSKANLNAALLLEALLVQW
ASLPGQR
>Mature_326_residues
AEAYPWQDSLWQQLAGRTQHAHAYLLHGPAGIGKRALAERLMASLLCQRPTPEACGECKSCLLLKAGSHPDNYILEPEEA
DKAIKVDQVRDLVSFVVQTAQLGGRKVVLIEPVESMNINAANALLKSLEEPSGDTVLLLVSHQPSRLLPTIKSRCVQQAC
PLPSESVSLQWLAQALPECSEDERVELLTLAAGSPLAAVTLQGQGVREQRAQVVEGVKKLLKQQQSPTQLAEEWKNIPML
RLFDWFCDWSSLILRYQLTQDEAGLGLTDMRKVVQYLAQKSAQPKVLDIQDWILAQRQIVLSKANLNAALLLEALLVQWA
SLPGQR

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=204, Percent_Identity=39.7058823529412, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1786676, Length=137, Percent_Identity=37.956204379562, Blast_Score=82, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 36187; Mature: 36056

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEAYPWQDSLWQQLAGRTQHAHAYLLHGPAGIGKRALAERLMASLLCQRPTPEACGECK
CCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
SCLLLKAGSHPDNYILEPEEADKAIKVDQVRDLVSFVVQTAQLGGRKVVLIEPVESMNIN
HEEEEECCCCCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHCCCC
AANALLKSLEEPSGDTVLLLVSHQPSRLLPTIKSRCVQQACPLPSESVSLQWLAQALPEC
HHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
SEDERVELLTLAAGSPLAAVTLQGQGVREQRAQVVEGVKKLLKQQQSPTQLAEEWKNIPM
CCCCCEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCH
LRLFDWFCDWSSLILRYQLTQDEAGLGLTDMRKVVQYLAQKSAQPKVLDIQDWILAQRQI
HHHHHHHHCHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHH
VLSKANLNAALLLEALLVQWASLPGQR
HHHHCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AEAYPWQDSLWQQLAGRTQHAHAYLLHGPAGIGKRALAERLMASLLCQRPTPEACGECK
CCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
SCLLLKAGSHPDNYILEPEEADKAIKVDQVRDLVSFVVQTAQLGGRKVVLIEPVESMNIN
HEEEEECCCCCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHCCCC
AANALLKSLEEPSGDTVLLLVSHQPSRLLPTIKSRCVQQACPLPSESVSLQWLAQALPEC
HHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
SEDERVELLTLAAGSPLAAVTLQGQGVREQRAQVVEGVKKLLKQQQSPTQLAEEWKNIPM
CCCCCEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCH
LRLFDWFCDWSSLILRYQLTQDEAGLGLTDMRKVVQYLAQKSAQPKVLDIQDWILAQRQI
HHHHHHHHCHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHH
VLSKANLNAALLLEALLVQWASLPGQR
HHHHCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]