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The map label for this gene is araF [H]

Identifier: 77460362

GI number: 77460362

Start: 4680216

End: 4681220

Strand: Reverse

Name: araF [H]

Synonym: Pfl01_4141

Alternate gene names: 77460362

Gene position: 4681220-4680216 (Counterclockwise)

Preceding gene: 77460363

Following gene: 77460361

Centisome position: 72.71

GC content: 61.79

Gene sequence:

>1005_bases
ATGAAACGTCGTTTCGGGATTCGTACCCTGTGCAGTACCGCGCTGGCGGTCACTGCGTTCAGCCTGAGCAGTGCCCTGCT
GGCCGCCGATCCGGTGAAGATCGGGTTTCTGGTCAAGCAGGCCGAAGAGCCGTGGTTCCAGACTGAATGGGCCTTCGCCG
AGAAAGCTGCCAAAGACAAAGGCTTCGAGCTGATCAAGATCGCCGTGCCGGACGGCGAGAAAACCCTCTCCGCTATCGAC
AGCCTTGCGGCCAACGGCGCCAAGGGCTTCGTCATCTGCCCGCCGGACGTGTCGCTCGGCCCGGCGATCATGGCCAAGGC
CAAGCTCAACGACCTGAAAGTGATCGCCGTCGACGACCGTTTCGTAGATGCCAACGGCAAGTTCATGGAAGACGTGCCGT
ACCTCGGCATGGCCGCCTTCGAAGTCGGCCAGAAGCAGGGTGCCGCCATGGCCGCCGAGGCGAAAAAGCGTAACTGGGAC
TGGAAAGACACCTACGCGGTGGTCAACACCTACAACGAACTCGACACCGGCAAGAAGCGCACCGACGGCTCGATGAAGGC
CCTCGAAGAGGCCGGCGTGCCGAAAGAGCACATTCTGACCGCCGCTCTGAAAACCCTCGACGTACCGGGCAGCATGGACG
CCACCAACTCGGCGCTGGTCAAGCTGCCGAGTGCGGCGAAGAACCTGATCATCGGCGGCATGAACGACAACACTGTGCTG
GGCGGCGTGCGCGCCACTGAAGCCGCCGGTTTTGCCGCGGCCAACGTGATCGGCATCGGCATCAACGGCACCGACGCGAT
CGGCGAGCTGAAAAAGCCCAACAGCGGCTTCTACGGTTCGATGCTGCCGAGCCCGCACATCGAAGGCTACAAGACCGCCG
AGATGATGTACGAGTGGGTCACCACCGGCAAAGAACCGCCGAAGTACACCGCAATGGACGACGTCACCCTGATCACCCGC
GACAACTTCAAGCAGGAGCTGGAGAAGATCGGTCTGTGGAACTGA

Upstream 100 bases:

>100_bases
ATCATACGATATGACTATTGTCGTCATGATTAGCAGGAACGCTTCGACCGCTTTAACGTTTTTCAAATAACGCTCAAGAA
AAATAACAAGGAGTCTGCTT

Downstream 100 bases:

>100_bases
CGGCAGCGGCGGCCCGGCCAGCCGGGCCGCTTGAACGGTGTGATGAGGTGGCTTAAATGCACGCGCAAGTACAGACACAA
GAACACAGCGCCAGCGGCAG

Product: periplasmic binding protein/LacI transcriptional regulator

Products: ADP; phosphate; arabinose [Cytoplasm] [C]

Alternate protein names: ABP [H]

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MKRRFGIRTLCSTALAVTAFSLSSALLAADPVKIGFLVKQAEEPWFQTEWAFAEKAAKDKGFELIKIAVPDGEKTLSAID
SLAANGAKGFVICPPDVSLGPAIMAKAKLNDLKVIAVDDRFVDANGKFMEDVPYLGMAAFEVGQKQGAAMAAEAKKRNWD
WKDTYAVVNTYNELDTGKKRTDGSMKALEEAGVPKEHILTAALKTLDVPGSMDATNSALVKLPSAAKNLIIGGMNDNTVL
GGVRATEAAGFAAANVIGIGINGTDAIGELKKPNSGFYGSMLPSPHIEGYKTAEMMYEWVTTGKEPPKYTAMDDVTLITR
DNFKQELEKIGLWN

Sequences:

>Translated_334_residues
MKRRFGIRTLCSTALAVTAFSLSSALLAADPVKIGFLVKQAEEPWFQTEWAFAEKAAKDKGFELIKIAVPDGEKTLSAID
SLAANGAKGFVICPPDVSLGPAIMAKAKLNDLKVIAVDDRFVDANGKFMEDVPYLGMAAFEVGQKQGAAMAAEAKKRNWD
WKDTYAVVNTYNELDTGKKRTDGSMKALEEAGVPKEHILTAALKTLDVPGSMDATNSALVKLPSAAKNLIIGGMNDNTVL
GGVRATEAAGFAAANVIGIGINGTDAIGELKKPNSGFYGSMLPSPHIEGYKTAEMMYEWVTTGKEPPKYTAMDDVTLITR
DNFKQELEKIGLWN
>Mature_334_residues
MKRRFGIRTLCSTALAVTAFSLSSALLAADPVKIGFLVKQAEEPWFQTEWAFAEKAAKDKGFELIKIAVPDGEKTLSAID
SLAANGAKGFVICPPDVSLGPAIMAKAKLNDLKVIAVDDRFVDANGKFMEDVPYLGMAAFEVGQKQGAAMAAEAKKRNWD
WKDTYAVVNTYNELDTGKKRTDGSMKALEEAGVPKEHILTAALKTLDVPGSMDATNSALVKLPSAAKNLIIGGMNDNTVL
GGVRATEAAGFAAANVIGIGINGTDAIGELKKPNSGFYGSMLPSPHIEGYKTAEMMYEWVTTGKEPPKYTAMDDVTLITR
DNFKQELEKIGLWN

Specific function: Involved in the high-affinity L-arabinose membrane transport system. Binds with high affinity to arabinose, but can also bind D-galactose (approximately 2-fold reduction) and D- fucose (approximately 40-fold reduction) [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788211, Length=320, Percent_Identity=60, Blast_Score=380, Evalue=1e-107,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35846; Mature: 35846

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRRFGIRTLCSTALAVTAFSLSSALLAADPVKIGFLVKQAEEPWFQTEWAFAEKAAKDK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHCCC
GFELIKIAVPDGEKTLSAIDSLAANGAKGFVICPPDVSLGPAIMAKAKLNDLKVIAVDDR
CCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHEEEECCCCEEEEEECCE
FVDANGKFMEDVPYLGMAAFEVGQKQGAAMAAEAKKRNWDWKDTYAVVNTYNELDTGKKR
EECCCCCCHHCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCHHHEEEEECHHHHCCCCCC
TDGSMKALEEAGVPKEHILTAALKTLDVPGSMDATNSALVKLPSAAKNLIIGGMNDNTVL
CCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCEEEECCCCCEEE
GGVRATEAAGFAAANVIGIGINGTDAIGELKKPNSGFYGSMLPSPHIEGYKTAEMMYEWV
CCCCHHHHCCCEEEEEEEECCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
TTGKEPPKYTAMDDVTLITRDNFKQELEKIGLWN
HCCCCCCCCCCCCCEEEEECCCHHHHHHHCCCCC
>Mature Secondary Structure
MKRRFGIRTLCSTALAVTAFSLSSALLAADPVKIGFLVKQAEEPWFQTEWAFAEKAAKDK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHCCC
GFELIKIAVPDGEKTLSAIDSLAANGAKGFVICPPDVSLGPAIMAKAKLNDLKVIAVDDR
CCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHEEEECCCCEEEEEECCE
FVDANGKFMEDVPYLGMAAFEVGQKQGAAMAAEAKKRNWDWKDTYAVVNTYNELDTGKKR
EECCCCCCHHCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCHHHEEEEECHHHHCCCCCC
TDGSMKALEEAGVPKEHILTAALKTLDVPGSMDATNSALVKLPSAAKNLIIGGMNDNTVL
CCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCEEEECCCCCEEE
GGVRATEAAGFAAANVIGIGINGTDAIGELKKPNSGFYGSMLPSPHIEGYKTAEMMYEWV
CCCCHHHHCCCEEEEEEEECCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
TTGKEPPKYTAMDDVTLITRDNFKQELEKIGLWN
HCCCCCCCCCCCCCEEEEECCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arabinose [Periplasm]; H2O [C]

Specific reaction: ATP + arabinose [Periplasm] + H2O = ADP + phosphate + arabinose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2445996; 9097040; 9278503; 6885805; 326784; 9298646; 7031057; 326785; 2204627 [H]