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The map label for this gene is asnB [H]

Identifier: 77460321

GI number: 77460321

Start: 4642018

End: 4643790

Strand: Direct

Name: asnB [H]

Synonym: Pfl01_4100

Alternate gene names: 77460321

Gene position: 4642018-4643790 (Clockwise)

Preceding gene: 77460319

Following gene: 77460322

Centisome position: 72.1

GC content: 62.44

Gene sequence:

>1773_bases
ATGTGCGGATTAGCTGGCGAGTTACGTTTTGATCAACAACCTGCAGACCTTGCAGCCATCGAGCGAATCACCCATCACCT
GGCGCCTCGCGGCCCCGACGCGTGGGGTTTCCATGCCCAAGGGCCGATTGCCCTGGGCCATCGACGCCTGAAAATCATGG
ACTTGTCGGACGGCTCGGCGCAGCCGATGATCGACAGTCAACTGGGCCTGTCCCTGGCCTTCAACGGCGCGATCTACAAC
TTCCCGGAACTGCGCGCCGAACTCGAAGCGCTCGGTTATGCGTTTTACTCCGGCGGCGACACCGAAGTGCTGCTCAAGGG
TTATCACGCCTGGGGCGAAGCGCTGCTGCCGAAACTCAACGGCATGTTTGCCTTCGCCATCTGGGAGCGTGATGCCCAGC
GTCTGTTCATTGCCCGCGACCGTCTCGGCGTGAAGCCGCTGTACCTGTCGCGCACCGGCCAGCGCCTGCGTTTTGCCTCG
GCCCTGCCAGCGCTGCTCAAGGGCGGTGACATCAACCCGATCCTCGATCCGGTGGCGCTCAACCATTACCTGAATTTCCA
CGCCGTGGTGCCTGCGCCGCGCACCTTGCTGGCGGGCATCGAAAAGCTGCCACCCGCGAGCTGGATGCGCATTGAAGCGG
ACGGCACCACCGAGCAGAAAACCTGGTGGACCCTGCCCTACGGCCCACACGCCGACGAGAAAAACCTGACCCTGGAAGAC
TGGCGTGACCGCGTACTCGACAGCACCCGCGAAGCCGTAGCCATCCGCCAACGGGCGGCGGTGGATGTCGGCGTGCTGCT
TTCCGGCGGCGTCGATTCGAGCATGCTCGTCGGCCTGTTACGCGAAGTCGGCGTGGAAAACCTGTCGACCTTTTCCATCG
GTTTTCAGGATGCCGGCGGCGAGCGCGGCGACGAATTCCAGTACTCCGATCTGATCGCCAAGCATTACGGTACGCAACAC
CATCAACTGCGCATCGACGAAAAAGAAATCATCGAGCAACTGCCCGCCGCGTTCCGCGCGATGAGCGAGCCGATGGTCAG
CCACGATTGCATCGCCTTCTATCTGCTGTCCCGCGAAGTGGCCAAGCACTGCAAGGTTGTGCAAAGCGGCCAGGGCGCCG
ACGAGTTGTTCGCCGGTTATCACTGGTATCCGCAAGTCGACGGTGCAGCCGATCCCTATGCGGCTTATCGCGAGGCATTT
TTCGACCGCAGCTACGACGACTATGCGGCCACGGTGCAGCCGAAATGGCTGACCGCCAATGACGCTGCCGGCGACTTCGT
GAAGGAACATTTCGCACAGCCCGGCGCCGATGCGGCGGTCGACAAGGCCCTGCGCCTGGACAGCACGGTGATGCTGGTGG
ACGACCCGGTCAAACGCGTCGACAACATGACCATGGCCTGGGGCCTGGAGGCGCGCACGCCGTTTCTCGACTATCGCCTG
GTGGAATTGTCGGCCCGCATTCCGGGCCAGTTCAAACTGCCGGACGGCGGCAAGCAGGTGTTGAAAGAAGCCGCGCGACG
GGTGATTCCGAGTGAAGTGATCGACCGCAAGAAAGGCTACTTCCCGGTGCCGGGTCTCAAGCATTTGCAGGGCGAGACCC
TGAGCTGGGTCCGCGAACTGCTGCTGGATCCGAGTCAGGATCGCGGCCTGTTCAACCCGGCCATGCTCGACCAATTGCTG
ACCGATCCACAAGGCCAGTTGACCCCGCTGCGCGGCTCCAAGCTGTGGCAACTGGCGGCTTTGAACCTGTGGCTCAGCGA
ACAAGGAATCTGA

Upstream 100 bases:

>100_bases
TCTAAAACTCCCACATCCGAGCCGGGTCAGTTTCTGGGTACCGGCCATTTCGGCGCGGTACAGCGACACCTTCCCCAACG
GAAAAACCGGTAAGGACTTT

Downstream 100 bases:

>100_bases
TTGATGAAACCTCACGCCACGGCTTACAGCCAGCGACTGCTGCGCGGCCAGACGCCGTCCTACGAACGCTTGCAGGCGCG
TCTGGCCGAAGATGGCAGCG

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 590; Mature: 590

Protein sequence:

>590_residues
MCGLAGELRFDQQPADLAAIERITHHLAPRGPDAWGFHAQGPIALGHRRLKIMDLSDGSAQPMIDSQLGLSLAFNGAIYN
FPELRAELEALGYAFYSGGDTEVLLKGYHAWGEALLPKLNGMFAFAIWERDAQRLFIARDRLGVKPLYLSRTGQRLRFAS
ALPALLKGGDINPILDPVALNHYLNFHAVVPAPRTLLAGIEKLPPASWMRIEADGTTEQKTWWTLPYGPHADEKNLTLED
WRDRVLDSTREAVAIRQRAAVDVGVLLSGGVDSSMLVGLLREVGVENLSTFSIGFQDAGGERGDEFQYSDLIAKHYGTQH
HQLRIDEKEIIEQLPAAFRAMSEPMVSHDCIAFYLLSREVAKHCKVVQSGQGADELFAGYHWYPQVDGAADPYAAYREAF
FDRSYDDYAATVQPKWLTANDAAGDFVKEHFAQPGADAAVDKALRLDSTVMLVDDPVKRVDNMTMAWGLEARTPFLDYRL
VELSARIPGQFKLPDGGKQVLKEAARRVIPSEVIDRKKGYFPVPGLKHLQGETLSWVRELLLDPSQDRGLFNPAMLDQLL
TDPQGQLTPLRGSKLWQLAALNLWLSEQGI

Sequences:

>Translated_590_residues
MCGLAGELRFDQQPADLAAIERITHHLAPRGPDAWGFHAQGPIALGHRRLKIMDLSDGSAQPMIDSQLGLSLAFNGAIYN
FPELRAELEALGYAFYSGGDTEVLLKGYHAWGEALLPKLNGMFAFAIWERDAQRLFIARDRLGVKPLYLSRTGQRLRFAS
ALPALLKGGDINPILDPVALNHYLNFHAVVPAPRTLLAGIEKLPPASWMRIEADGTTEQKTWWTLPYGPHADEKNLTLED
WRDRVLDSTREAVAIRQRAAVDVGVLLSGGVDSSMLVGLLREVGVENLSTFSIGFQDAGGERGDEFQYSDLIAKHYGTQH
HQLRIDEKEIIEQLPAAFRAMSEPMVSHDCIAFYLLSREVAKHCKVVQSGQGADELFAGYHWYPQVDGAADPYAAYREAF
FDRSYDDYAATVQPKWLTANDAAGDFVKEHFAQPGADAAVDKALRLDSTVMLVDDPVKRVDNMTMAWGLEARTPFLDYRL
VELSARIPGQFKLPDGGKQVLKEAARRVIPSEVIDRKKGYFPVPGLKHLQGETLSWVRELLLDPSQDRGLFNPAMLDQLL
TDPQGQLTPLRGSKLWQLAALNLWLSEQGI
>Mature_590_residues
MCGLAGELRFDQQPADLAAIERITHHLAPRGPDAWGFHAQGPIALGHRRLKIMDLSDGSAQPMIDSQLGLSLAFNGAIYN
FPELRAELEALGYAFYSGGDTEVLLKGYHAWGEALLPKLNGMFAFAIWERDAQRLFIARDRLGVKPLYLSRTGQRLRFAS
ALPALLKGGDINPILDPVALNHYLNFHAVVPAPRTLLAGIEKLPPASWMRIEADGTTEQKTWWTLPYGPHADEKNLTLED
WRDRVLDSTREAVAIRQRAAVDVGVLLSGGVDSSMLVGLLREVGVENLSTFSIGFQDAGGERGDEFQYSDLIAKHYGTQH
HQLRIDEKEIIEQLPAAFRAMSEPMVSHDCIAFYLLSREVAKHCKVVQSGQGADELFAGYHWYPQVDGAADPYAAYREAF
FDRSYDDYAATVQPKWLTANDAAGDFVKEHFAQPGADAAVDKALRLDSTVMLVDDPVKRVDNMTMAWGLEARTPFLDYRL
VELSARIPGQFKLPDGGKQVLKEAARRVIPSEVIDRKKGYFPVPGLKHLQGETLSWVRELLLDPSQDRGLFNPAMLDQLL
TDPQGQLTPLRGSKLWQLAALNLWLSEQGI

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786889, Length=570, Percent_Identity=26.140350877193, Blast_Score=149, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI17560178, Length=537, Percent_Identity=24.7672253258845, Blast_Score=116, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6321563, Length=649, Percent_Identity=24.9614791987673, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6325403, Length=613, Percent_Identity=25.7748776508972, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI45553209, Length=566, Percent_Identity=24.5583038869258, Blast_Score=119, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 65575; Mature: 65575

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGLAGELRFDQQPADLAAIERITHHLAPRGPDAWGFHAQGPIALGHRRLKIMDLSDGSA
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCEEEEEECCCCCC
QPMIDSQLGLSLAFNGAIYNFPELRAELEALGYAFYSGGDTEVLLKGYHAWGEALLPKLN
CCCCHHCCCEEEEECCCCCCCHHHHHHHHHHHHHEECCCCCEEEEEHHHHHHHHHHHHHC
GMFAFAIWERDAQRLFIARDRLGVKPLYLSRTGQRLRFASALPALLKGGDINPILDPVAL
CEEEEEEECCCHHHEEEEHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHH
NHYLNFHAVVPAPRTLLAGIEKLPPASWMRIEADGTTEQKTWWTLPYGPHADEKNLTLED
HHHCCEEEECCCCHHHHHHHHHCCCCCEEEEECCCCCCCCEEEECCCCCCCCCCCCCHHH
WRDRVLDSTREAVAIRQRAAVDVGVLLSGGVDSSMLVGLLREVGVENLSTFSIGFQDAGG
HHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCC
ERGDEFQYSDLIAKHYGTQHHQLRIDEKEIIEQLPAAFRAMSEPMVSHDCIAFYLLSREV
CCCCCCHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
AKHCKVVQSGQGADELFAGYHWYPQVDGAADPYAAYREAFFDRSYDDYAATVQPKWLTAN
HHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEECCCCEEECC
DAAGDFVKEHFAQPGADAAVDKALRLDSTVMLVDDPVKRVDNMTMAWGLEARTPFLDYRL
CCHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEECCHHHHHCCCEEEECCCCCCCCHHEEE
VELSARIPGQFKLPDGGKQVLKEAARRVIPSEVIDRKKGYFPVPGLKHLQGETLSWVREL
EEECCCCCCCEECCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCCCHHHHCCCHHHHHHHH
LLDPSQDRGLFNPAMLDQLLTDPQGQLTPLRGSKLWQLAALNLWLSEQGI
HCCCCCCCCCCCHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MCGLAGELRFDQQPADLAAIERITHHLAPRGPDAWGFHAQGPIALGHRRLKIMDLSDGSA
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCEEEEEECCCCCC
QPMIDSQLGLSLAFNGAIYNFPELRAELEALGYAFYSGGDTEVLLKGYHAWGEALLPKLN
CCCCHHCCCEEEEECCCCCCCHHHHHHHHHHHHHEECCCCCEEEEEHHHHHHHHHHHHHC
GMFAFAIWERDAQRLFIARDRLGVKPLYLSRTGQRLRFASALPALLKGGDINPILDPVAL
CEEEEEEECCCHHHEEEEHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHH
NHYLNFHAVVPAPRTLLAGIEKLPPASWMRIEADGTTEQKTWWTLPYGPHADEKNLTLED
HHHCCEEEECCCCHHHHHHHHHCCCCCEEEEECCCCCCCCEEEECCCCCCCCCCCCCHHH
WRDRVLDSTREAVAIRQRAAVDVGVLLSGGVDSSMLVGLLREVGVENLSTFSIGFQDAGG
HHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCC
ERGDEFQYSDLIAKHYGTQHHQLRIDEKEIIEQLPAAFRAMSEPMVSHDCIAFYLLSREV
CCCCCCHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
AKHCKVVQSGQGADELFAGYHWYPQVDGAADPYAAYREAFFDRSYDDYAATVQPKWLTAN
HHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEECCCCEEECC
DAAGDFVKEHFAQPGADAAVDKALRLDSTVMLVDDPVKRVDNMTMAWGLEARTPFLDYRL
CCHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEECCHHHHHCCCEEEECCCCCCCCHHEEE
VELSARIPGQFKLPDGGKQVLKEAARRVIPSEVIDRKKGYFPVPGLKHLQGETLSWVREL
EEECCCCCCCEECCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCCCHHHHCCCHHHHHHHH
LLDPSQDRGLFNPAMLDQLLTDPQGQLTPLRGSKLWQLAALNLWLSEQGI
HCCCCCCCCCCCHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]