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The map label for this gene is mnmC

Identifier: 77460319

GI number: 77460319

Start: 4634886

End: 4636865

Strand: Direct

Name: mnmC

Synonym: Pfl01_4098

Alternate gene names: 77460319

Gene position: 4634886-4636865 (Clockwise)

Preceding gene: 77460316

Following gene: 77460321

Centisome position: 71.99

GC content: 63.54

Gene sequence:

>1980_bases
ATGAAACCTGTAATGCCCCACGCCCAACTCGACTGGGATGACCAGGGACGCCCGCGTTCGCGAGTGTTCGACGATGTGTA
TTTTTCCGACCAGTCGGGGCTGGATGAAACCCGCTACGTGTTCCTTGAACAGAACCGTCTGGCCGAGCGTTTTGCCGCGT
TGTCGGCGGGTGGGCGACTGGTGATCGGTGAAACCGGCTTCGGCACCGGGCTGAATTTTCTCTGCGCCTGGCAGTTGTTC
GAACAGCACGCGGTGGCCGGTGCGCGACTGCATTTTGTCAGCGTCGAAAAATTTCCGTTGAGCCCTGCCGACCTGCAACG
GGCGCTGGCGTTGTGGCCCGACCTGAAGCGATTCTCCGATCAGTTGCTCAAGCATTACGTGGCAATCCATCAGGGATTTC
AGCGCATTGTTCTGGATAACGGGCGCATAACCCTGACCCTGTTGATCGGTGACGCGCTGGAGCAGTTGCCACAACTGGAC
GGCCAGATCGACGCCTGGTTTCTCGACGGTTTCGCGCCAGCGAAAAACCCCGAGATGTGGACCGCCGAACTGTTCGCCGA
ACTGGCCCGGCTGGCGGCACCCGGCTCGACCATCAGCACGTTTACCAGCACCGGTTGGGTGCGACGCCTGCTCAACGCCG
CCGGCTTCAAGATGAAACGCACGCCGGGCATCGGCCACAAATGGGAAATCCTGCGCGGCGAATTTCTCGGCTGGCCCGAG
GGTGTTGCGCCACCTGCGGCGGCAAAACCGTGGTTCGCCCGCCCCACACGGTTGACAGGTGACCGTCGAGCCCTGGTGAT
CGGCGCCGGTCTGGCCGGTTGCGCCACGGCGGCCAGCCTCGCGGCGCGGGGCTGGCAGGTCAGTCTGCTGGAACGGCATG
ATGCGGTGGCCCAGGAAGCCTCGGGTAACCCGCAAGGCGTTCTGTATCTGAAACTGTCCGCCCATGGCACGGCACTTTCG
CAGTTGATCGTCAGCGGTTTCGGTTACACCCGGCGCGTGCTGGAAACCTTGCAGCGTGGCACCGACTGGGATGATTGCGG
GGTGCTGCAACTGGCGTTCAATGAGAAGGAGCGCGAGCGTCAGGCACAACTGGCATCCGCGTTTCCCGAAGACCTGCTGC
AATGGCTCGATCAACCCGAAGCGCAGGCCCGTGCCGGAGTCGGTCTGGCTCACGGCGGTTTGTATTACCCCGAGGGTGGC
TGGGTACATCCGCCGGCGTTGTGTCAGGCGCAATCGGCACAGCCGGGCGTCACACTGCTCCCCCATCAGGAAGCCGTCGA
GTTGCGCAAAGTGGGCGATCAATGGCAAGCCTTCGACGGTGAACGATTGATTGCAACAGCGCCCGTCGTGGTGCTGGCCG
GTGCCGCCGAAATCAAGCGTTTTGCCCAAAGCGGCGAGCTGCCCCTCAAGCGCATTCGCGGGCAGATCACCCGCCTGGCG
CAGACTGCGCAAAGCCAGGCGCTGGCCACTGTCGTGTGTGCCGAAGGTTATGTCGCACCGGCACGATGGGGCGAGCACAC
CCTCGGCGCCAGTTTCGACTTCAGCAGCGACGACCTGACCCCGACCACCGCCGAACACCTGGGCAATCTGGCGATGCTCG
AAGAGATCTCCACCGATCTGGTCGCGCGCCTGCACGTCAGCGAGCGCGACGCCGAAAGTCTTCAGGGCCGGGCAGCCTTC
CGTTGCACCAGCCCCGATTACCTGCCGATGGTCGGCCCGCTCGCCGACCGCGAAGCGTTTACCCGGATCTACGCCGCATT
GAGCAAGGATGCACGGCAGGTGCCGGACATGCCTTGTCCCTGGCTCGACGGACTGTACGTCAACAGCGGTCATGGCTCCC
GAGGTCTGATCACCGCACCCTTGTCCGGTGAACTGCTCGCGGCCTGGCTGGACAACGAACCGCTGCCCCTGCCCAGAAGT
GTCGCAGAGGCGTGTCATCCGAACCGGTTTGCCTTGCGGCGACTGATCCGGGGCAAATGA

Upstream 100 bases:

>100_bases
GGCGGATTGTCCTTGAGTCATCTGTGGCAAGGTTTGCCATGGATCAAGCGCCGCCCGCGTGATCGGCTAGAATGGCCGCC
TTGCCGTTGCCGAGCCTGCC

Downstream 100 bases:

>100_bases
GCGGATCATCGGGAGAATGATCAACCAGCGGGCAACACGCAGAGGTTGCCCTCCAGCAGCCGCAACACCTGGTCGGTCAG
TTGCTCGAGCAAGGCGCTTT

Product: 5-methylaminomethyl-2-thiouridine methyltransferase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase

Number of amino acids: Translated: 659; Mature: 659

Protein sequence:

>659_residues
MKPVMPHAQLDWDDQGRPRSRVFDDVYFSDQSGLDETRYVFLEQNRLAERFAALSAGGRLVIGETGFGTGLNFLCAWQLF
EQHAVAGARLHFVSVEKFPLSPADLQRALALWPDLKRFSDQLLKHYVAIHQGFQRIVLDNGRITLTLLIGDALEQLPQLD
GQIDAWFLDGFAPAKNPEMWTAELFAELARLAAPGSTISTFTSTGWVRRLLNAAGFKMKRTPGIGHKWEILRGEFLGWPE
GVAPPAAAKPWFARPTRLTGDRRALVIGAGLAGCATAASLAARGWQVSLLERHDAVAQEASGNPQGVLYLKLSAHGTALS
QLIVSGFGYTRRVLETLQRGTDWDDCGVLQLAFNEKERERQAQLASAFPEDLLQWLDQPEAQARAGVGLAHGGLYYPEGG
WVHPPALCQAQSAQPGVTLLPHQEAVELRKVGDQWQAFDGERLIATAPVVVLAGAAEIKRFAQSGELPLKRIRGQITRLA
QTAQSQALATVVCAEGYVAPARWGEHTLGASFDFSSDDLTPTTAEHLGNLAMLEEISTDLVARLHVSERDAESLQGRAAF
RCTSPDYLPMVGPLADREAFTRIYAALSKDARQVPDMPCPWLDGLYVNSGHGSRGLITAPLSGELLAAWLDNEPLPLPRS
VAEACHPNRFALRRLIRGK

Sequences:

>Translated_659_residues
MKPVMPHAQLDWDDQGRPRSRVFDDVYFSDQSGLDETRYVFLEQNRLAERFAALSAGGRLVIGETGFGTGLNFLCAWQLF
EQHAVAGARLHFVSVEKFPLSPADLQRALALWPDLKRFSDQLLKHYVAIHQGFQRIVLDNGRITLTLLIGDALEQLPQLD
GQIDAWFLDGFAPAKNPEMWTAELFAELARLAAPGSTISTFTSTGWVRRLLNAAGFKMKRTPGIGHKWEILRGEFLGWPE
GVAPPAAAKPWFARPTRLTGDRRALVIGAGLAGCATAASLAARGWQVSLLERHDAVAQEASGNPQGVLYLKLSAHGTALS
QLIVSGFGYTRRVLETLQRGTDWDDCGVLQLAFNEKERERQAQLASAFPEDLLQWLDQPEAQARAGVGLAHGGLYYPEGG
WVHPPALCQAQSAQPGVTLLPHQEAVELRKVGDQWQAFDGERLIATAPVVVLAGAAEIKRFAQSGELPLKRIRGQITRLA
QTAQSQALATVVCAEGYVAPARWGEHTLGASFDFSSDDLTPTTAEHLGNLAMLEEISTDLVARLHVSERDAESLQGRAAF
RCTSPDYLPMVGPLADREAFTRIYAALSKDARQVPDMPCPWLDGLYVNSGHGSRGLITAPLSGELLAAWLDNEPLPLPRS
VAEACHPNRFALRRLIRGK
>Mature_659_residues
MKPVMPHAQLDWDDQGRPRSRVFDDVYFSDQSGLDETRYVFLEQNRLAERFAALSAGGRLVIGETGFGTGLNFLCAWQLF
EQHAVAGARLHFVSVEKFPLSPADLQRALALWPDLKRFSDQLLKHYVAIHQGFQRIVLDNGRITLTLLIGDALEQLPQLD
GQIDAWFLDGFAPAKNPEMWTAELFAELARLAAPGSTISTFTSTGWVRRLLNAAGFKMKRTPGIGHKWEILRGEFLGWPE
GVAPPAAAKPWFARPTRLTGDRRALVIGAGLAGCATAASLAARGWQVSLLERHDAVAQEASGNPQGVLYLKLSAHGTALS
QLIVSGFGYTRRVLETLQRGTDWDDCGVLQLAFNEKERERQAQLASAFPEDLLQWLDQPEAQARAGVGLAHGGLYYPEGG
WVHPPALCQAQSAQPGVTLLPHQEAVELRKVGDQWQAFDGERLIATAPVVVLAGAAEIKRFAQSGELPLKRIRGQITRLA
QTAQSQALATVVCAEGYVAPARWGEHTLGASFDFSSDDLTPTTAEHLGNLAMLEEISTDLVARLHVSERDAESLQGRAAF
RCTSPDYLPMVGPLADREAFTRIYAALSKDARQVPDMPCPWLDGLYVNSGHGSRGLITAPLSGELLAAWLDNEPLPLPRS
VAEACHPNRFALRRLIRGK

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family

Homologues:

Organism=Escherichia coli, GI87082084, Length=669, Percent_Identity=39.6113602391629, Blast_Score=424, Evalue=1e-119,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMC_PSEPF (Q3K8R9)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_349826.1
- ProteinModelPortal:   Q3K8R9
- SMR:   Q3K8R9
- STRING:   Q3K8R9
- GeneID:   3712402
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_4098
- eggNOG:   COG0665
- HOGENOM:   HBG677640
- OMA:   VIGETGF
- ProtClustDB:   PRK01747
- BioCyc:   PFLU205922:PFL_4098-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01102
- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610
- TIGRFAMs:   TIGR03197

Pfam domain/function: PF01266 DAO; PF05430 DUF752

EC number: =2.1.1.61

Molecular weight: Translated: 72140; Mature: 72140

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPVMPHAQLDWDDQGRPRSRVFDDVYFSDQSGLDETRYVFLEQNRLAERFAALSAGGRL
CCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHCCCCEE
VIGETGFGTGLNFLCAWQLFEQHAVAGARLHFVSVEKFPLSPADLQRALALWPDLKRFSD
EEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHCHHHHHHHH
QLLKHYVAIHQGFQRIVLDNGRITLTLLIGDALEQLPQLDGQIDAWFLDGFAPAKNPEMW
HHHHHHHHHHHCCCEEEEECCCEEEEEEECHHHHHHHHCCCCEEEEEECCCCCCCCCCHH
TAELFAELARLAAPGSTISTFTSTGWVRRLLNAAGFKMKRTPGIGHKWEILRGEFLGWPE
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEECCCCCCCCC
GVAPPAAAKPWFARPTRLTGDRRALVIGAGLAGCATAASLAARGWQVSLLERHDAVAQEA
CCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHC
SGNPQGVLYLKLSAHGTALSQLIVSGFGYTRRVLETLQRGTDWDDCGVLQLAFNEKERER
CCCCCEEEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHH
QAQLASAFPEDLLQWLDQPEAQARAGVGLAHGGLYYPEGGWVHPPALCQAQSAQPGVTLL
HHHHHHHCHHHHHHHHCCCHHHHHCCCEEECCCEECCCCCCCCCHHHHCCCCCCCCEEEC
PHQEAVELRKVGDQWQAFDGERLIATAPVVVLAGAAEIKRFAQSGELPLKRIRGQITRLA
CCHHHHHHHHCCCHHHCCCCCEEEEECCHHEECCHHHHHHHHHCCCCHHHHHHHHHHHHH
QTAQSQALATVVCAEGYVAPARWGEHTLGASFDFSSDDLTPTTAEHLGNLAMLEEISTDL
HHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHH
VARLHVSERDAESLQGRAAFRCTSPDYLPMVGPLADREAFTRIYAALSKDARQVPDMPCP
HHHHHCCCCCHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
WLDGLYVNSGHGSRGLITAPLSGELLAAWLDNEPLPLPRSVAEACHPNRFALRRLIRGK
CCCCEEEECCCCCCCEEECCCCCCEEEEECCCCCCCCCHHHHHHCCCCHHHHHHHHCCC
>Mature Secondary Structure
MKPVMPHAQLDWDDQGRPRSRVFDDVYFSDQSGLDETRYVFLEQNRLAERFAALSAGGRL
CCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHCCCCEE
VIGETGFGTGLNFLCAWQLFEQHAVAGARLHFVSVEKFPLSPADLQRALALWPDLKRFSD
EEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHCHHHHHHHH
QLLKHYVAIHQGFQRIVLDNGRITLTLLIGDALEQLPQLDGQIDAWFLDGFAPAKNPEMW
HHHHHHHHHHHCCCEEEEECCCEEEEEEECHHHHHHHHCCCCEEEEEECCCCCCCCCCHH
TAELFAELARLAAPGSTISTFTSTGWVRRLLNAAGFKMKRTPGIGHKWEILRGEFLGWPE
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEECCCCCCCCC
GVAPPAAAKPWFARPTRLTGDRRALVIGAGLAGCATAASLAARGWQVSLLERHDAVAQEA
CCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHC
SGNPQGVLYLKLSAHGTALSQLIVSGFGYTRRVLETLQRGTDWDDCGVLQLAFNEKERER
CCCCCEEEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHH
QAQLASAFPEDLLQWLDQPEAQARAGVGLAHGGLYYPEGGWVHPPALCQAQSAQPGVTLL
HHHHHHHCHHHHHHHHCCCHHHHHCCCEEECCCEECCCCCCCCCHHHHCCCCCCCCEEEC
PHQEAVELRKVGDQWQAFDGERLIATAPVVVLAGAAEIKRFAQSGELPLKRIRGQITRLA
CCHHHHHHHHCCCHHHCCCCCEEEEECCHHEECCHHHHHHHHHCCCCHHHHHHHHHHHHH
QTAQSQALATVVCAEGYVAPARWGEHTLGASFDFSSDDLTPTTAEHLGNLAMLEEISTDL
HHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHH
VARLHVSERDAESLQGRAAFRCTSPDYLPMVGPLADREAFTRIYAALSKDARQVPDMPCP
HHHHHCCCCCHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
WLDGLYVNSGHGSRGLITAPLSGELLAAWLDNEPLPLPRSVAEACHPNRFALRRLIRGK
CCCCEEEECCCCCCCEEECCCCCCEEEEECCCCCCCCCHHHHHHCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA