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The map label for this gene is fadA [H]

Identifier: 77460317

GI number: 77460317

Start: 4631912

End: 4633096

Strand: Reverse

Name: fadA [H]

Synonym: Pfl01_4096

Alternate gene names: 77460317

Gene position: 4633096-4631912 (Counterclockwise)

Preceding gene: 77460318

Following gene: 77460315

Centisome position: 71.96

GC content: 62.11

Gene sequence:

>1185_bases
ATGCGTGAAGTGGTGATCGTCGACAGCGTACGGACCGGCCTGGCCAAGTCCTTTCGCGGCAAGTTCAACCAGACCCGTCC
CGATGACATGGCGGCCCATTGCGTCAATGCGTTGCTCTCGCGCAACGACATCGACCCGGCCAGCGTCGAGGATTGCATCG
TCGGCGCCGGCTCTAACGAAGGCGCTCAGGGCTACAACATCGGCCGCAACGTGGCGGTGCTCTCGCAACTGGGCACCGGC
ACCGCCGGCATGACCCTCAATCGTTTCTGTTCGTCGGGTTTGCAGGCAATTGCGATTGCCGCCAACCAGATCGCGTCGGG
TTGCAGCGACATCATCGTCGCCGGCGGCGTCGAGTCGATCAGCCTGACCCTGAAAAGCGTCAACACCGATCACCTGATCA
ACCCGTTGCTCAAGCAGCAGACGCCGGGCATCTACTACACCATGGGCCAGACTGCCGAAGTGGTGGCGCGCCGTTATGGC
GTCAGCCGCGAGGCGCAGGATCGTTACTCGCTGCAAAGTCAGATCCGCACCGCCCAGGCGCAGGCAGCCGGCCTGTTCAA
CGATGAAATCGTGCCGATGGCGGTGAAGTACCGCGTTGAAGACAAGAACAGCGGTGACGTGCAGATCCTCGACGGCATCG
TCGATCGCGACGACTGCAACCGCCCGGACACCACCTATGAAAGCCTCGCCGGGTTGAAACCGGTGTTCGCCGAGGACGGG
TCGGTGACCGCCGGCAACTCGTCGCAGTTGTCCGACGGCGCCTCGATGACGCTGGTGATGAGCCTGGAAAAAGCCCTGCA
ACTGGGGCTCAAGCCGAAAGCATTTTTCCGTGGGTTTACCGTGGCCGGGTGCGAGCCGGACGAAATGGGCATCGGCCCGG
TGTTCTCGGTGCCGAAACTGCTCAAGGCCAAAGGCTTGCAGGTGGCGGATATCGATCTGTGGGAATTGAACGAAGCGTTT
GCCTCGCAGTGCCTGTATGCCCGTGATCGTCTGGAAATCGACAACGAGAAGTACAACGTCAATGGCGGCTCGATTTCCAT
CGGCCACCCGTTCGGCATGACCGGGTCACGGCAGGTCGGGCATCTGGTGCGCGAGTTGCAGCGGCGCAATCTGCGCTACG
GAATCGTGACCATGTGTGTGGGCGGGGGGATGGGCGCGACCGGGTTGTTTGAGGCCGTACGCTAG

Upstream 100 bases:

>100_bases
CGATGGGCCCGCATACGCGAGGTGAATGATTGTCGCGGTCGGCGATTGGGTGGACTTATGCTCGGTCCACTCTCAACCGA
CTGCAACAATGAGGTATGCC

Downstream 100 bases:

>100_bases
GCAGGCCTGCCGGAATCCATCAATCGCATCCGCGCGATGTACGCGCGGATTTCCTGCTCGGCTTTCTCGGGGCTGTCGAA
CGGCCCTTCGAGGGTGTTTT

Product: Acetyl-CoA C-acyltransferase

Products: NA

Alternate protein names: Acetyl-CoA acyltransferase; Beta-ketothiolase [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MREVVIVDSVRTGLAKSFRGKFNQTRPDDMAAHCVNALLSRNDIDPASVEDCIVGAGSNEGAQGYNIGRNVAVLSQLGTG
TAGMTLNRFCSSGLQAIAIAANQIASGCSDIIVAGGVESISLTLKSVNTDHLINPLLKQQTPGIYYTMGQTAEVVARRYG
VSREAQDRYSLQSQIRTAQAQAAGLFNDEIVPMAVKYRVEDKNSGDVQILDGIVDRDDCNRPDTTYESLAGLKPVFAEDG
SVTAGNSSQLSDGASMTLVMSLEKALQLGLKPKAFFRGFTVAGCEPDEMGIGPVFSVPKLLKAKGLQVADIDLWELNEAF
ASQCLYARDRLEIDNEKYNVNGGSISIGHPFGMTGSRQVGHLVRELQRRNLRYGIVTMCVGGGMGATGLFEAVR

Sequences:

>Translated_394_residues
MREVVIVDSVRTGLAKSFRGKFNQTRPDDMAAHCVNALLSRNDIDPASVEDCIVGAGSNEGAQGYNIGRNVAVLSQLGTG
TAGMTLNRFCSSGLQAIAIAANQIASGCSDIIVAGGVESISLTLKSVNTDHLINPLLKQQTPGIYYTMGQTAEVVARRYG
VSREAQDRYSLQSQIRTAQAQAAGLFNDEIVPMAVKYRVEDKNSGDVQILDGIVDRDDCNRPDTTYESLAGLKPVFAEDG
SVTAGNSSQLSDGASMTLVMSLEKALQLGLKPKAFFRGFTVAGCEPDEMGIGPVFSVPKLLKAKGLQVADIDLWELNEAF
ASQCLYARDRLEIDNEKYNVNGGSISIGHPFGMTGSRQVGHLVRELQRRNLRYGIVTMCVGGGMGATGLFEAVR
>Mature_394_residues
MREVVIVDSVRTGLAKSFRGKFNQTRPDDMAAHCVNALLSRNDIDPASVEDCIVGAGSNEGAQGYNIGRNVAVLSQLGTG
TAGMTLNRFCSSGLQAIAIAANQIASGCSDIIVAGGVESISLTLKSVNTDHLINPLLKQQTPGIYYTMGQTAEVVARRYG
VSREAQDRYSLQSQIRTAQAQAAGLFNDEIVPMAVKYRVEDKNSGDVQILDGIVDRDDCNRPDTTYESLAGLKPVFAEDG
SVTAGNSSQLSDGASMTLVMSLEKALQLGLKPKAFFRGFTVAGCEPDEMGIGPVFSVPKLLKAKGLQVADIDLWELNEAF
ASQCLYARDRLEIDNEKYNVNGGSISIGHPFGMTGSRQVGHLVRELQRRNLRYGIVTMCVGGGMGATGLFEAVR

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4501853, Length=391, Percent_Identity=43.4782608695652, Blast_Score=289, Evalue=3e-78,
Organism=Homo sapiens, GI167614485, Length=399, Percent_Identity=35.8395989974937, Blast_Score=239, Evalue=5e-63,
Organism=Homo sapiens, GI4557237, Length=400, Percent_Identity=33, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI4504327, Length=438, Percent_Identity=33.7899543378995, Blast_Score=202, Evalue=3e-52,
Organism=Homo sapiens, GI148539872, Length=394, Percent_Identity=33.502538071066, Blast_Score=201, Evalue=8e-52,
Organism=Homo sapiens, GI194353979, Length=390, Percent_Identity=32.8205128205128, Blast_Score=165, Evalue=5e-41,
Organism=Escherichia coli, GI48994986, Length=401, Percent_Identity=38.9027431421446, Blast_Score=271, Evalue=4e-74,
Organism=Escherichia coli, GI1787663, Length=416, Percent_Identity=37.9807692307692, Blast_Score=258, Evalue=6e-70,
Organism=Escherichia coli, GI87082165, Length=396, Percent_Identity=38.8888888888889, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1788554, Length=405, Percent_Identity=37.7777777777778, Blast_Score=238, Evalue=7e-64,
Organism=Escherichia coli, GI1788683, Length=424, Percent_Identity=30.188679245283, Blast_Score=169, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI133906874, Length=401, Percent_Identity=36.9077306733167, Blast_Score=246, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI25147385, Length=397, Percent_Identity=31.4861460957179, Blast_Score=195, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI17535921, Length=404, Percent_Identity=32.1782178217822, Blast_Score=188, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI17551802, Length=442, Percent_Identity=29.4117647058824, Blast_Score=177, Evalue=7e-45,
Organism=Caenorhabditis elegans, GI17535917, Length=394, Percent_Identity=27.1573604060914, Blast_Score=131, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6322031, Length=397, Percent_Identity=38.0352644836272, Blast_Score=239, Evalue=6e-64,
Organism=Saccharomyces cerevisiae, GI6325229, Length=410, Percent_Identity=29.7560975609756, Blast_Score=167, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24655093, Length=397, Percent_Identity=36.5239294710327, Blast_Score=243, Evalue=2e-64,
Organism=Drosophila melanogaster, GI17648125, Length=321, Percent_Identity=40.4984423676012, Blast_Score=221, Evalue=5e-58,
Organism=Drosophila melanogaster, GI17137578, Length=436, Percent_Identity=35.3211009174312, Blast_Score=216, Evalue=3e-56,
Organism=Drosophila melanogaster, GI24640423, Length=399, Percent_Identity=33.0827067669173, Blast_Score=211, Evalue=6e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 42154; Mature: 42154

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREVVIVDSVRTGLAKSFRGKFNQTRPDDMAAHCVNALLSRNDIDPASVEDCIVGAGSNE
CCCEEEECCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHEEECCCCC
GAQGYNIGRNVAVLSQLGTGTAGMTLNRFCSSGLQAIAIAANQIASGCSDIIVAGGVESI
CCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEECCCCEE
SLTLKSVNTDHLINPLLKQQTPGIYYTMGQTAEVVARRYGVSREAQDRYSLQSQIRTAQA
EEEEEECCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
QAAGLFNDEIVPMAVKYRVEDKNSGDVQILDGIVDRDDCNRPDTTYESLAGLKPVFAEDG
HHHCCCCCCCEEEEEEEEECCCCCCCEEEEECCCCCCCCCCCCCHHHHHHCCCCEEECCC
SVTAGNSSQLSDGASMTLVMSLEKALQLGLKPKAFFRGFTVAGCEPDEMGIGPVFSVPKL
CEECCCCCCCCCCCCEEHHHHHHHHHHHCCCCHHHHCCCEEECCCCCCCCCCCHHHHHHH
LKAKGLQVADIDLWELNEAFASQCLYARDRLEIDNEKYNVNGGSISIGHPFGMTGSRQVG
HHHCCCEEEECHHHHHHHHHHHHHHHHHHCCEECCCEEECCCCEEEECCCCCCCCCHHHH
HLVRELQRRNLRYGIVTMCVGGGMGATGLFEAVR
HHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHCC
>Mature Secondary Structure
MREVVIVDSVRTGLAKSFRGKFNQTRPDDMAAHCVNALLSRNDIDPASVEDCIVGAGSNE
CCCEEEECCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHEEECCCCC
GAQGYNIGRNVAVLSQLGTGTAGMTLNRFCSSGLQAIAIAANQIASGCSDIIVAGGVESI
CCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEECCCCEE
SLTLKSVNTDHLINPLLKQQTPGIYYTMGQTAEVVARRYGVSREAQDRYSLQSQIRTAQA
EEEEEECCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
QAAGLFNDEIVPMAVKYRVEDKNSGDVQILDGIVDRDDCNRPDTTYESLAGLKPVFAEDG
HHHCCCCCCCEEEEEEEEECCCCCCCEEEEECCCCCCCCCCCCCHHHHHHCCCCEEECCC
SVTAGNSSQLSDGASMTLVMSLEKALQLGLKPKAFFRGFTVAGCEPDEMGIGPVFSVPKL
CEECCCCCCCCCCCCEEHHHHHHHHHHHCCCCHHHHCCCEEECCCCCCCCCCCHHHHHHH
LKAKGLQVADIDLWELNEAFASQCLYARDRLEIDNEKYNVNGGSISIGHPFGMTGSRQVG
HHHCCCEEEECHHHHHHHHHHHHHHHHHHCCEECCCEEECCCCEEEECCCCCCCCCHHHH
HLVRELQRRNLRYGIVTMCVGGGMGATGLFEAVR
HHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]