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The map label for this gene is dosC [H]

Identifier: 77460305

GI number: 77460305

Start: 4615908

End: 4616834

Strand: Direct

Name: dosC [H]

Synonym: Pfl01_4084

Alternate gene names: 77460305

Gene position: 4615908-4616834 (Clockwise)

Preceding gene: 77460304

Following gene: 77460308

Centisome position: 71.69

GC content: 62.24

Gene sequence:

>927_bases
ATGAAATCACCCTCCCAGACCACTGCAATTGACTTCGACAGTGCCAAATTGCAACGCCTGGGCTTTGGTCAGCAGCCGCC
TCTCTTGGCGCGACCTGTCAGCCTGGCGCAATTGCGCCAGCAAATGAGCCTGCAACTGCAAACCAGCCTTGAGCCTCAAC
GCATCCTCGGTCTGTTTTTCCGCGAAGTTCAGCGTCTGGTGCCGCTGGACGCCTTGACCTACGTGCACAACGGTTGCGAC
CTGCGCCTGGAGTTCGGTGCCCGCGGCCACCACTCGGCCAGCTACCGCCTCAGCCACGAAGGCGAGCACATGGGTGAGCT
GGTGTTCCGCCGCAACCAGCGCTTCAGCGAACAGGAACTGGGCAATCTCGAATCGTTGCTGTCTGCGCTGCTGTATCCGA
TGCGCAATGCCCTGCTGTACCGCGCCGCCACGCAAAGCGCGCTGCGTGATCCGCTGACCGGTGCCGGCAACCGCATTGCC
ATGGAGCAGACGCTGCACCGCGAAATCGAGATGTCCCGCCGGCACCTGCAACCGCTGTCGTTGCTGATGCTGGACATCGA
CCACTTCAAGCAGGTCAACGACCTCCATGGTCACAGCGCCGGCGACGACGTGCTCAAAGCTGTGGCCGCATCGATCAAGG
GCCAGTTGCGCAACGTCGACATGGTCTTCCGTTATGGCGGGGAAGAGTTTCTGATCCTGTTGTCCAACACCGGTCGCGAC
GCGGCCGCCATGGTCGGTGAGCGCCTGCGTCAGGCAGCCCAGGCCCAGGATTACTACGCCAATAACCAATTGATCGAACT
GACCGTGAGCCTCGGCTGTTCGACCCTGTTGCCGGGTGAATCTGCCGACAGCCTGTTGCGCCGCGCCGACAGCGCGCTGT
ATGTCGCCAAGCGTGAGGGCCGCAATCGCCTGACCATGGCGGGCTAG

Upstream 100 bases:

>100_bases
CCAAATGTCCTACGGGACTGATGGATCAGTATGACGCGCAGCCCATAGCCGCTCTCACCCAGCCTGTAAGACAGACTTAC
TGCTTAGGGGCTCACGCCAT

Downstream 100 bases:

>100_bases
ATATTCGCTGCCAACAAAAAACGGGAGCCAGGGCTCCCGTTTTTTTTATTGATGTCGCCGAAACTAGCTCTCGACCAGTG
CCCGACGCTCACGCCCGACC

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MKSPSQTTAIDFDSAKLQRLGFGQQPPLLARPVSLAQLRQQMSLQLQTSLEPQRILGLFFREVQRLVPLDALTYVHNGCD
LRLEFGARGHHSASYRLSHEGEHMGELVFRRNQRFSEQELGNLESLLSALLYPMRNALLYRAATQSALRDPLTGAGNRIA
MEQTLHREIEMSRRHLQPLSLLMLDIDHFKQVNDLHGHSAGDDVLKAVAASIKGQLRNVDMVFRYGGEEFLILLSNTGRD
AAAMVGERLRQAAQAQDYYANNQLIELTVSLGCSTLLPGESADSLLRRADSALYVAKREGRNRLTMAG

Sequences:

>Translated_308_residues
MKSPSQTTAIDFDSAKLQRLGFGQQPPLLARPVSLAQLRQQMSLQLQTSLEPQRILGLFFREVQRLVPLDALTYVHNGCD
LRLEFGARGHHSASYRLSHEGEHMGELVFRRNQRFSEQELGNLESLLSALLYPMRNALLYRAATQSALRDPLTGAGNRIA
MEQTLHREIEMSRRHLQPLSLLMLDIDHFKQVNDLHGHSAGDDVLKAVAASIKGQLRNVDMVFRYGGEEFLILLSNTGRD
AAAMVGERLRQAAQAQDYYANNQLIELTVSLGCSTLLPGESADSLLRRADSALYVAKREGRNRLTMAG
>Mature_308_residues
MKSPSQTTAIDFDSAKLQRLGFGQQPPLLARPVSLAQLRQQMSLQLQTSLEPQRILGLFFREVQRLVPLDALTYVHNGCD
LRLEFGARGHHSASYRLSHEGEHMGELVFRRNQRFSEQELGNLESLLSALLYPMRNALLYRAATQSALRDPLTGAGNRIA
MEQTLHREIEMSRRHLQPLSLLMLDIDHFKQVNDLHGHSAGDDVLKAVAASIKGQLRNVDMVFRYGGEEFLILLSNTGRD
AAAMVGERLRQAAQAQDYYANNQLIELTVSLGCSTLLPGESADSLLRRADSALYVAKREGRNRLTMAG

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=158, Percent_Identity=37.3417721518987, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI87081881, Length=166, Percent_Identity=40.9638554216867, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1786584, Length=205, Percent_Identity=33.6585365853659, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787816, Length=156, Percent_Identity=36.5384615384615, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1787262, Length=173, Percent_Identity=35.2601156069364, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI87082007, Length=179, Percent_Identity=35.195530726257, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787802, Length=203, Percent_Identity=30.5418719211823, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1788381, Length=170, Percent_Identity=31.1764705882353, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787541, Length=166, Percent_Identity=34.9397590361446, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87081974, Length=165, Percent_Identity=27.2727272727273, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87081977, Length=151, Percent_Identity=31.7880794701987, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788085, Length=187, Percent_Identity=26.7379679144385, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788956, Length=173, Percent_Identity=31.7919075144509, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 34434; Mature: 34434

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSPSQTTAIDFDSAKLQRLGFGQQPPLLARPVSLAQLRQQMSLQLQTSLEPQRILGLFF
CCCCCCCCEEECCHHHHHHCCCCCCCCCHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
REVQRLVPLDALTYVHNGCDLRLEFGARGHHSASYRLSHEGEHMGELVFRRNQRFSEQEL
HHHHHHCCHHHHHHHHCCCEEEEECCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCHHHH
GNLESLLSALLYPMRNALLYRAATQSALRDPLTGAGNRIAMEQTLHREIEMSRRHLQPLS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHCCHHH
LLMLDIDHFKQVNDLHGHSAGDDVLKAVAASIKGQLRNVDMVFRYGGEEFLILLSNTGRD
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCEEEEEEECCCCH
AAAMVGERLRQAAQAQDYYANNQLIELTVSLGCSTLLPGESADSLLRRADSALYVAKREG
HHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCHHHHHHHHCHHEEEEHHCC
RNRLTMAG
CCCCCCCC
>Mature Secondary Structure
MKSPSQTTAIDFDSAKLQRLGFGQQPPLLARPVSLAQLRQQMSLQLQTSLEPQRILGLFF
CCCCCCCCEEECCHHHHHHCCCCCCCCCHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
REVQRLVPLDALTYVHNGCDLRLEFGARGHHSASYRLSHEGEHMGELVFRRNQRFSEQEL
HHHHHHCCHHHHHHHHCCCEEEEECCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCHHHH
GNLESLLSALLYPMRNALLYRAATQSALRDPLTGAGNRIAMEQTLHREIEMSRRHLQPLS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHCCHHH
LLMLDIDHFKQVNDLHGHSAGDDVLKAVAASIKGQLRNVDMVFRYGGEEFLILLSNTGRD
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCEEEEEEECCCCH
AAAMVGERLRQAAQAQDYYANNQLIELTVSLGCSTLLPGESADSLLRRADSALYVAKREG
HHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCHHHHHHHHCHHEEEEHHCC
RNRLTMAG
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]