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The map label for this gene is gph2 [H]

Identifier: 77460303

GI number: 77460303

Start: 4614053

End: 4614724

Strand: Direct

Name: gph2 [H]

Synonym: Pfl01_4082

Alternate gene names: 77460303

Gene position: 4614053-4614724 (Clockwise)

Preceding gene: 77460302

Following gene: 77460304

Centisome position: 71.66

GC content: 61.9

Gene sequence:

>672_bases
ATGGCCATCAGAGCAGTCCTTTTCGACATGGACGGCACTCTGCTCGACACCGCGCCGGACTTTATCGCCATCTGCCAGGC
GATGCGCGCGGATCGTGGCTTGCCGCCGATGAACGACAAACACATCCGCGATGAAATCTCCGGCGGCGCCAGGGCCATGG
TCGCGGTGACGTTCTCGATGGATCCGGAGTCGCCGGGCTTCGAGGAACTGCGTCTTGAGTTCCTCGAACGCTATCTCGTC
GGTTGCGCCGTGCACAGCAAGCTGTTCGACGGCATGGGCGAATTGCTGGCCGACATCGAGAAGGCCAATCTGGTCTGGGG
CGTGGTCACCAACAAGCCGCTGCGCTTCGCCGAGCCAATCATGCGGCAACTGGGGCTGGCCGAACGCTCTGCCCTGTTGA
TCTGCCCGGATCACGTGAAGAACAGCAAGCCGGATCCCGAGATGCTGACGCTGGCCTGCAAGATGCTTGATCTGGATCCG
TCGACCGTGCTGTTTATCGGCGATGATCTGCGCGATATCGAATCCGGCCGTGACGCCGGCACCAGGACCGCAGCCGTGAC
CTATGGCTACATTCACCCCGACGACAACCCTCGGCACTGGGGCGCGGACGTGGTGGTGGATCATCCGCTGGAGTTGCGCA
AGGTGCTGGATAACGCTCTGTGCAGTTGCTGA

Upstream 100 bases:

>100_bases
ATCATCGGCCTGACTTACAACCCGCTGACCAAGCACTACAAGCTGGCGGCGGACGTTGACGTCAACTACATGATCCAGAC
CCTGCGCGAGGAGTAAGCCG

Downstream 100 bases:

>100_bases
GTGGTGCTGCAAAGCGACCATCGCTGCATTCCCGCGCAAGACGCGGGAGCGATCAGAAGTTAATGGATTAGAGGTTTCTT
ATGTTTGATTACTCCGCTCG

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP 2; PGPase2 [H]

Number of amino acids: Translated: 223; Mature: 222

Protein sequence:

>223_residues
MAIRAVLFDMDGTLLDTAPDFIAICQAMRADRGLPPMNDKHIRDEISGGARAMVAVTFSMDPESPGFEELRLEFLERYLV
GCAVHSKLFDGMGELLADIEKANLVWGVVTNKPLRFAEPIMRQLGLAERSALLICPDHVKNSKPDPEMLTLACKMLDLDP
STVLFIGDDLRDIESGRDAGTRTAAVTYGYIHPDDNPRHWGADVVVDHPLELRKVLDNALCSC

Sequences:

>Translated_223_residues
MAIRAVLFDMDGTLLDTAPDFIAICQAMRADRGLPPMNDKHIRDEISGGARAMVAVTFSMDPESPGFEELRLEFLERYLV
GCAVHSKLFDGMGELLADIEKANLVWGVVTNKPLRFAEPIMRQLGLAERSALLICPDHVKNSKPDPEMLTLACKMLDLDP
STVLFIGDDLRDIESGRDAGTRTAAVTYGYIHPDDNPRHWGADVVVDHPLELRKVLDNALCSC
>Mature_222_residues
AIRAVLFDMDGTLLDTAPDFIAICQAMRADRGLPPMNDKHIRDEISGGARAMVAVTFSMDPESPGFEELRLEFLERYLVG
CAVHSKLFDGMGELLADIEKANLVWGVVTNKPLRFAEPIMRQLGLAERSALLICPDHVKNSKPDPEMLTLACKMLDLDPS
TVLFIGDDLRDIESGRDAGTRTAAVTYGYIHPDDNPRHWGADVVVDHPLELRKVLDNALCSC

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=210, Percent_Identity=30, Blast_Score=96, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 24635; Mature: 24503

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIRAVLFDMDGTLLDTAPDFIAICQAMRADRGLPPMNDKHIRDEISGGARAMVAVTFSM
CCEEEEEECCCCCEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCEEEEEEEEEC
DPESPGFEELRLEFLERYLVGCAVHSKLFDGMGELLADIEKANLVWGVVTNKPLRFAEPI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHH
MRQLGLAERSALLICPDHVKNSKPDPEMLTLACKMLDLDPSTVLFIGDDLRDIESGRDAG
HHHHCCCCCCEEEEECHHHCCCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHCCCCCC
TRTAAVTYGYIHPDDNPRHWGADVVVDHPLELRKVLDNALCSC
CEEEEEEEEEECCCCCCCCCCCCEEECCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
AIRAVLFDMDGTLLDTAPDFIAICQAMRADRGLPPMNDKHIRDEISGGARAMVAVTFSM
CEEEEEECCCCCEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCEEEEEEEEEC
DPESPGFEELRLEFLERYLVGCAVHSKLFDGMGELLADIEKANLVWGVVTNKPLRFAEPI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHH
MRQLGLAERSALLICPDHVKNSKPDPEMLTLACKMLDLDPSTVLFIGDDLRDIESGRDAG
HHHHCCCCCCEEEEECHHHCCCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHCCCCCC
TRTAAVTYGYIHPDDNPRHWGADVVVDHPLELRKVLDNALCSC
CEEEEEEEEEECCCCCCCCCCCCEEECCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]