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The map label for this gene is cysB [H]

Identifier: 77460224

GI number: 77460224

Start: 4530356

End: 4531330

Strand: Reverse

Name: cysB [H]

Synonym: Pfl01_4003

Alternate gene names: 77460224

Gene position: 4531330-4530356 (Counterclockwise)

Preceding gene: 77460225

Following gene: 77460221

Centisome position: 70.38

GC content: 59.49

Gene sequence:

>975_bases
ATGAAGCTTCAACAACTGCGCTACATCTGGGAAGTGGCGCACCACGACCTCAACGTTTCCGCTACAGCCCAAAGCCTCTA
CACCTCGCAACCGGGCATCAGTAAACAGATCCGTCTGCTGGAAGACGAACTGGGCGTCGAAGTGTTCGCCCGCAGCGGCA
AGCACCTGACCCGCGTCACTCCGGCCGGCGAGCGCATCATCACCACTGCCGGTGAGATTCTGCGCAAGGTCGAAAGCATC
AAGCAGATCGCCCAGGAATTCTCCAACGAGAAGAAAGGCACCCTGTCGATCGCCACCACCCATACCCAGGCACGTTATGC
ACTGCCGCCGGTGATCAGCAATTTCATCAAGCAATATCCGGACGTGGCGCTGCATATGCACCAGGGTTCGCCGATGCAGA
TCGCCGAAATGGCCGCTGACGGCACCGTCGATTTCGCCATCGCCACCGAAGCGCTGGAGCTGTTCGGCGATCTGGTGATG
ATGCCGTGCTATCGCTGGAACCGTTGCGTGGTCGTGCCTCAGGGCCACCCGCTGACCAAACTGCCGAAGCTGACCCTCGA
AGCGCTGGCCGAATACCCGATCGTGACTTACGTGTTCGGTTTCACCGGCCGTTCGAAACTCGACGAAGCCTTCAGCCATC
GCGGCCTGACGCCGAAAGTGGTGTTCACCGCTGCCGATGCCGACGTGATCAAAACCTACGTGCGCCTGGGCCTGGGTGTT
GGCATCGTGGCGAAAATGGCGGTCGACACCAAGCTGGATAACGATCTGGTGGTGCTCGATGCCAGCGAACTGTTCGAGTC
CAGCGTGACCAAGATCGGTTTCCGTCGCGGTACTTTCCTGCGCGGCTTCATGTGCGACTTCATCGAGAAGTTCGCGCCGC
ACCTGACCCGCGAAGTCATGGCCAAGGCCATTCAGTGCCACAACAAGCAGGAGCTGGAAGAGCTGTTCGAAGGCGTCGAA
CTGCCGGTTCACTAA

Upstream 100 bases:

>100_bases
GCACTTTGATAAAAAAGTTCTAGATTTATTCTTCAAACCATTAATATAGTTATATACCGTCGCTGATGCCCGTGGCGTCT
ACCTGCTTTGAGGGATTCAT

Downstream 100 bases:

>100_bases
GCCCCTGTCTACAGCACCTCGGTGACAGCAAACTGTTGCCGGGTGCCCGCCACCAGAATCTCCACCTCGTCCCCCTCAAA
CTTGCCCAGCAGGCTTTTGC

Product: transcriptional regulator CysB

Products: NA

Alternate protein names: Cys regulon transcriptional activator [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKLQQLRYIWEVAHHDLNVSATAQSLYTSQPGISKQIRLLEDELGVEVFARSGKHLTRVTPAGERIITTAGEILRKVESI
KQIAQEFSNEKKGTLSIATTHTQARYALPPVISNFIKQYPDVALHMHQGSPMQIAEMAADGTVDFAIATEALELFGDLVM
MPCYRWNRCVVVPQGHPLTKLPKLTLEALAEYPIVTYVFGFTGRSKLDEAFSHRGLTPKVVFTAADADVIKTYVRLGLGV
GIVAKMAVDTKLDNDLVVLDASELFESSVTKIGFRRGTFLRGFMCDFIEKFAPHLTREVMAKAIQCHNKQELEELFEGVE
LPVH

Sequences:

>Translated_324_residues
MKLQQLRYIWEVAHHDLNVSATAQSLYTSQPGISKQIRLLEDELGVEVFARSGKHLTRVTPAGERIITTAGEILRKVESI
KQIAQEFSNEKKGTLSIATTHTQARYALPPVISNFIKQYPDVALHMHQGSPMQIAEMAADGTVDFAIATEALELFGDLVM
MPCYRWNRCVVVPQGHPLTKLPKLTLEALAEYPIVTYVFGFTGRSKLDEAFSHRGLTPKVVFTAADADVIKTYVRLGLGV
GIVAKMAVDTKLDNDLVVLDASELFESSVTKIGFRRGTFLRGFMCDFIEKFAPHLTREVMAKAIQCHNKQELEELFEGVE
LPVH
>Mature_324_residues
MKLQQLRYIWEVAHHDLNVSATAQSLYTSQPGISKQIRLLEDELGVEVFARSGKHLTRVTPAGERIITTAGEILRKVESI
KQIAQEFSNEKKGTLSIATTHTQARYALPPVISNFIKQYPDVALHMHQGSPMQIAEMAADGTVDFAIATEALELFGDLVM
MPCYRWNRCVVVPQGHPLTKLPKLTLEALAEYPIVTYVFGFTGRSKLDEAFSHRGLTPKVVFTAADADVIKTYVRLGLGV
GIVAKMAVDTKLDNDLVVLDASELFESSVTKIGFRRGTFLRGFMCDFIEKFAPHLTREVMAKAIQCHNKQELEELFEGVE
LPVH

Specific function: This protein is a positive regulator of gene expression for the cysteine regulon. The inducer for cysB is N-acetylserine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=322, Percent_Identity=63.9751552795031, Blast_Score=443, Evalue=1e-126,
Organism=Escherichia coli, GI1788296, Length=313, Percent_Identity=41.2140575079872, Blast_Score=272, Evalue=3e-74,
Organism=Escherichia coli, GI157672245, Length=235, Percent_Identity=29.7872340425532, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1790399, Length=258, Percent_Identity=24.8062015503876, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787879, Length=253, Percent_Identity=29.2490118577075, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788887, Length=253, Percent_Identity=28.0632411067194, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787601, Length=149, Percent_Identity=29.5302013422819, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI2367136, Length=242, Percent_Identity=22.7272727272727, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1788481, Length=258, Percent_Identity=27.1317829457364, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790262, Length=191, Percent_Identity=27.7486910994764, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1789204, Length=269, Percent_Identity=24.5353159851301, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1790208, Length=269, Percent_Identity=21.9330855018587, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36129; Mature: 36129

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQQLRYIWEVAHHDLNVSATAQSLYTSQPGISKQIRLLEDELGVEVFARSGKHLTRVT
CCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHCCCCCEEEEC
PAGERIITTAGEILRKVESIKQIAQEFSNEKKGTLSIATTHTQARYALPPVISNFIKQYP
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHCCCHHHHHHHHHCC
DVALHMHQGSPMQIAEMAADGTVDFAIATEALELFGDLVMMPCYRWNRCVVVPQGHPLTK
CEEEEECCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCH
LPKLTLEALAEYPIVTYVFGFTGRSKLDEAFSHRGLTPKVVFTAADADVIKTYVRLGLGV
HHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHCCH
GIVAKMAVDTKLDNDLVVLDASELFESSVTKIGFRRGTFLRGFMCDFIEKFAPHLTREVM
HHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
AKAIQCHNKQELEELFEGVELPVH
HHHHHHCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKLQQLRYIWEVAHHDLNVSATAQSLYTSQPGISKQIRLLEDELGVEVFARSGKHLTRVT
CCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHCCCCCEEEEC
PAGERIITTAGEILRKVESIKQIAQEFSNEKKGTLSIATTHTQARYALPPVISNFIKQYP
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHCCCHHHHHHHHHCC
DVALHMHQGSPMQIAEMAADGTVDFAIATEALELFGDLVMMPCYRWNRCVVVPQGHPLTK
CEEEEECCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCH
LPKLTLEALAEYPIVTYVFGFTGRSKLDEAFSHRGLTPKVVFTAADADVIKTYVRLGLGV
HHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHCCH
GIVAKMAVDTKLDNDLVVLDASELFESSVTKIGFRRGTFLRGFMCDFIEKFAPHLTREVM
HHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
AKAIQCHNKQELEELFEGVELPVH
HHHHHHCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]