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The map label for this gene is gacS [H]

Identifier: 77460200

GI number: 77460200

Start: 4507744

End: 4510080

Strand: Direct

Name: gacS [H]

Synonym: Pfl01_3979

Alternate gene names: 77460200

Gene position: 4507744-4510080 (Clockwise)

Preceding gene: 77460197

Following gene: 77460204

Centisome position: 70.01

GC content: 61.53

Gene sequence:

>2337_bases
GTGGTTCAACACCCCATGGATATCAAATTCACCCACCGGCTGTCGTACAAACAGGCGCGGCTTACCGTGCTGGTCGGGTT
CATTCTGGGCACGCTGCTCAGCCTGCTGCAAATCGGCATCGATTATGCCAGTGAAGACGCGTCCATCAATCGTGAAATCC
TGTCTTTGTTGGAAATCAGCCATAACCCGGCCTCACGCATCGCCTACAACATCGATGCCGAACTGGCGCAGGAGCTGACC
CTGGGCCTGCTGCGCTCGCCGGCCATCATTTCTGCAAGACTCACCGATAACAACCAGACCGTATTGGCCAGCGTCAAACG
TCCGGAGCTGCAAAGCGGCTACCGGATGATCAGCGACTTCCTGTTCGGCGCCAAACGTCAGTTCGAAGACCGTCTTTATC
TCGATCACTTGCCCAACGAATCCCTCGGCACCCTGAGCCTGGAAGTCGATACCTTCGCCTTTGGCAGCCGCTTTCTGCGC
CGCGCGGAAGTCACGCTGCTCAACGGTTTCGCCCGCAGCCTGATCCTGACCGGGATCCTGCTCGCGCTGTTCTACGTGAT
GCTGACCAAACCCCTGGTGCGAGTGATCCGTGAACTCAGCGGCCGCGACCCGCGCAGTGCCGAGCCGACCACCCTGGAAT
GTCCGGGCGGGCACGCCAATGACGAGATCGGCGTGCTGGTCAAAGTTGCCAATCAGCAGTTCGAGAACATCGCCACGGAA
ATCCAGCAACGGCGCAATGCGGAAAACCGTCTCACCGACTACCTCGCACAACTCGAAGACATCGTCTCGGCGCGTACCGC
CGAGCTCAAGGCGATCAACGCGCGACTCAGCCAGTCCAACCAGGAACTGGAAGTGGCCCGTAGTACGGCACTGGACATGG
CCGAGGCTCGCTCGGCATTCCTCGCCAACATGAGCCATGAGATCCGTACCCCGCTCAACGGCTTGCTGGGAATGATCGCT
CTGTCGCTCGATGGTCCGCTCAACGCCGAACAGCAGCAACAACTGTCCATCGCCCACGACTCCGGCAAGGTATTGGTGGA
ATTGCTCAACGACATTCTCGACCTGTCCAAATTCGATGCCGGGCAGCTGGAGCTCGAACACATTCCCTTCGATCTCGGCT
CACTGATCGAAGACACTGCCAACCTGCTGTCGCAAAACGCGGCGCCGAGTGTGGAACTGACCTGCCTGATCGATCCGCAC
TTCCCGGCGCTGGTGCTTGGCGATCCGACCCGGGTCAGGCAAATCGTCAGCAACCTGCTGTCCAATGCCCTGAAATTCAC
TCGTTTCGGCCGGGTCGATGTGCGTTTGTCCACCTGGAAGGACGGCGTCCGGATCGAGGTCTGCGACACCGGCATCGGCA
TCGCCCAGGAAGCGCAGGTGAAGATTTTCCAGCCGTTTACCCAGGCGGGTGCCGGGATCACTCGCCAGTACGGCGGCACC
GGGCTCGGTCTGGCACTGACCTATAACCTGTGCGAAGCCATGCAGGGGCGTCTGACCATCAGTTCCGAAGTGGGTTTCGG
CAGTCAGTTCTGCGCTGAGCTGCCACTGCCCTGCCACACCCGCGCGGTGATTCCACCCCCGTTGCACGGAAAGGTGCTGG
CGATCACCAACGCGGTCAGCGGGCTGGCGGAGCTGCTGGAAAGCCTGCTGCCGGTCTGGGGGCTGACCTACGAACAGCGA
ACCCTCGACGACCCGCTGCTCGGCCTGTCCCCCGACGTCATCATCACCGATTGCCCCGAATGCCTGTTCGGTCTGCGTCC
GGCGGTGACGGCGCCGATTCTGCTGGTGACGGCTTATGGCAGTTTCATGCCGGGTGAAGAAGCCACTGCCCTTGCCCCAT
TGCAGCAGCAGGCTCGACCGCTGGCCCGCAACGCGCTGTATCAGAATCTGCGTCGGACACTGCAGCCGGACCTCACCACC
ATCGACCGCGCACGCCTCGAAAGCGTGCCGTCGCAGCGGCGCGGTCGGGTACTGCTGGTGGAGGACAACCCGGTCAATCA
ACTGGTGGCCAAGGGCATGCTGGGCAAACTCGGCTGCGATGTGGTGGTTGCCGCCCATGGCGTGGAGGCGCTGGATCAGT
TGGAACACGACGAGTTCGATCTGGTACTGATGGACTGCAACATGCCGGTGATGGACGGTTACGAAGCCAGCCGCCAGATT
CGTCAAAGCGGACGCTGGCCGCATCTGCCGATCGTCGCCCTGACCGCCAACGCCATGTCCGAGGAGCGCGAACGTTGCCG
GGCGGCGGGCATGAGCGACTATCTGGCCAAGCCGTTCCGTCGTGAAGAATTGGCGGCGCTGCTGGACCAGTGGATTCCGA
CTACGACAGTGCTTTGA

Upstream 100 bases:

>100_bases
ACAGCGTTCGAAACCTGTCCCGGGCCTTGTTACAAGGCGCTTCGGGAACCCCGGAATCCGTGCATGATCGACTATGCTTG
AGCAGCGAAGGGAAACGGAA

Downstream 100 bases:

>100_bases
TCTGCCCCAGCAAGTGATCAAGACCGCTACGCAAGTCATTGAGCCGGTCCAGATCAACCCCGCTGTCGCACAACAGCCGC
GCCTTCAACGGCCCGACCTG

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 778; Mature: 778

Protein sequence:

>778_residues
MVQHPMDIKFTHRLSYKQARLTVLVGFILGTLLSLLQIGIDYASEDASINREILSLLEISHNPASRIAYNIDAELAQELT
LGLLRSPAIISARLTDNNQTVLASVKRPELQSGYRMISDFLFGAKRQFEDRLYLDHLPNESLGTLSLEVDTFAFGSRFLR
RAEVTLLNGFARSLILTGILLALFYVMLTKPLVRVIRELSGRDPRSAEPTTLECPGGHANDEIGVLVKVANQQFENIATE
IQQRRNAENRLTDYLAQLEDIVSARTAELKAINARLSQSNQELEVARSTALDMAEARSAFLANMSHEIRTPLNGLLGMIA
LSLDGPLNAEQQQQLSIAHDSGKVLVELLNDILDLSKFDAGQLELEHIPFDLGSLIEDTANLLSQNAAPSVELTCLIDPH
FPALVLGDPTRVRQIVSNLLSNALKFTRFGRVDVRLSTWKDGVRIEVCDTGIGIAQEAQVKIFQPFTQAGAGITRQYGGT
GLGLALTYNLCEAMQGRLTISSEVGFGSQFCAELPLPCHTRAVIPPPLHGKVLAITNAVSGLAELLESLLPVWGLTYEQR
TLDDPLLGLSPDVIITDCPECLFGLRPAVTAPILLVTAYGSFMPGEEATALAPLQQQARPLARNALYQNLRRTLQPDLTT
IDRARLESVPSQRRGRVLLVEDNPVNQLVAKGMLGKLGCDVVVAAHGVEALDQLEHDEFDLVLMDCNMPVMDGYEASRQI
RQSGRWPHLPIVALTANAMSEERERCRAAGMSDYLAKPFRREELAALLDQWIPTTTVL

Sequences:

>Translated_778_residues
MVQHPMDIKFTHRLSYKQARLTVLVGFILGTLLSLLQIGIDYASEDASINREILSLLEISHNPASRIAYNIDAELAQELT
LGLLRSPAIISARLTDNNQTVLASVKRPELQSGYRMISDFLFGAKRQFEDRLYLDHLPNESLGTLSLEVDTFAFGSRFLR
RAEVTLLNGFARSLILTGILLALFYVMLTKPLVRVIRELSGRDPRSAEPTTLECPGGHANDEIGVLVKVANQQFENIATE
IQQRRNAENRLTDYLAQLEDIVSARTAELKAINARLSQSNQELEVARSTALDMAEARSAFLANMSHEIRTPLNGLLGMIA
LSLDGPLNAEQQQQLSIAHDSGKVLVELLNDILDLSKFDAGQLELEHIPFDLGSLIEDTANLLSQNAAPSVELTCLIDPH
FPALVLGDPTRVRQIVSNLLSNALKFTRFGRVDVRLSTWKDGVRIEVCDTGIGIAQEAQVKIFQPFTQAGAGITRQYGGT
GLGLALTYNLCEAMQGRLTISSEVGFGSQFCAELPLPCHTRAVIPPPLHGKVLAITNAVSGLAELLESLLPVWGLTYEQR
TLDDPLLGLSPDVIITDCPECLFGLRPAVTAPILLVTAYGSFMPGEEATALAPLQQQARPLARNALYQNLRRTLQPDLTT
IDRARLESVPSQRRGRVLLVEDNPVNQLVAKGMLGKLGCDVVVAAHGVEALDQLEHDEFDLVLMDCNMPVMDGYEASRQI
RQSGRWPHLPIVALTANAMSEERERCRAAGMSDYLAKPFRREELAALLDQWIPTTTVL
>Mature_778_residues
MVQHPMDIKFTHRLSYKQARLTVLVGFILGTLLSLLQIGIDYASEDASINREILSLLEISHNPASRIAYNIDAELAQELT
LGLLRSPAIISARLTDNNQTVLASVKRPELQSGYRMISDFLFGAKRQFEDRLYLDHLPNESLGTLSLEVDTFAFGSRFLR
RAEVTLLNGFARSLILTGILLALFYVMLTKPLVRVIRELSGRDPRSAEPTTLECPGGHANDEIGVLVKVANQQFENIATE
IQQRRNAENRLTDYLAQLEDIVSARTAELKAINARLSQSNQELEVARSTALDMAEARSAFLANMSHEIRTPLNGLLGMIA
LSLDGPLNAEQQQQLSIAHDSGKVLVELLNDILDLSKFDAGQLELEHIPFDLGSLIEDTANLLSQNAAPSVELTCLIDPH
FPALVLGDPTRVRQIVSNLLSNALKFTRFGRVDVRLSTWKDGVRIEVCDTGIGIAQEAQVKIFQPFTQAGAGITRQYGGT
GLGLALTYNLCEAMQGRLTISSEVGFGSQFCAELPLPCHTRAVIPPPLHGKVLAITNAVSGLAELLESLLPVWGLTYEQR
TLDDPLLGLSPDVIITDCPECLFGLRPAVTAPILLVTAYGSFMPGEEATALAPLQQQARPLARNALYQNLRRTLQPDLTT
IDRARLESVPSQRRGRVLLVEDNPVNQLVAKGMLGKLGCDVVVAAHGVEALDQLEHDEFDLVLMDCNMPVMDGYEASRQI
RQSGRWPHLPIVALTANAMSEERERCRAAGMSDYLAKPFRREELAALLDQWIPTTTVL

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=561, Percent_Identity=33.1550802139037, Blast_Score=258, Evalue=1e-69,
Organism=Escherichia coli, GI145693157, Length=496, Percent_Identity=35.0806451612903, Blast_Score=243, Evalue=3e-65,
Organism=Escherichia coli, GI87081816, Length=373, Percent_Identity=36.1930294906166, Blast_Score=172, Evalue=6e-44,
Organism=Escherichia coli, GI1788713, Length=272, Percent_Identity=35.6617647058824, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI48994928, Length=230, Percent_Identity=38.2608695652174, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI1786600, Length=243, Percent_Identity=32.9218106995885, Blast_Score=96, Evalue=1e-20,
Organism=Escherichia coli, GI1788393, Length=242, Percent_Identity=30.1652892561983, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI87082128, Length=261, Percent_Identity=29.5019157088123, Blast_Score=93, Evalue=7e-20,
Organism=Escherichia coli, GI1790436, Length=218, Percent_Identity=31.1926605504587, Blast_Score=91, Evalue=4e-19,
Organism=Escherichia coli, GI1788549, Length=271, Percent_Identity=26.1992619926199, Blast_Score=80, Evalue=6e-16,
Organism=Escherichia coli, GI1786783, Length=227, Percent_Identity=28.1938325991189, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI1790346, Length=232, Percent_Identity=28.448275862069, Blast_Score=79, Evalue=2e-15,
Organism=Escherichia coli, GI1787894, Length=228, Percent_Identity=29.3859649122807, Blast_Score=73, Evalue=7e-14,
Organism=Escherichia coli, GI1790300, Length=258, Percent_Identity=27.1317829457364, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1788191, Length=116, Percent_Identity=34.4827586206897, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1789808, Length=234, Percent_Identity=26.4957264957265, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6322000, Length=138, Percent_Identity=36.231884057971, Blast_Score=85, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6322044, Length=374, Percent_Identity=24.8663101604278, Blast_Score=81, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 85576; Mature: 85576

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQHPMDIKFTHRLSYKQARLTVLVGFILGTLLSLLQIGIDYASEDASINREILSLLEIS
CCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHC
HNPASRIAYNIDAELAQELTLGLLRSPAIISARLTDNNQTVLASVKRPELQSGYRMISDF
CCCHHHEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCCCHHHHHHHHHHH
LFGAKRQFEDRLYLDHLPNESLGTLSLEVDTFAFGSRFLRRAEVTLLNGFARSLILTGIL
HHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LALFYVMLTKPLVRVIRELSGRDPRSAEPTTLECPGGHANDEIGVLVKVANQQFENIATE
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCEEEEEEHHHHHHHHHHH
IQQRRNAENRLTDYLAQLEDIVSARTAELKAINARLSQSNQELEVARSTALDMAEARSAF
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LANMSHEIRTPLNGLLGMIALSLDGPLNAEQQQQLSIAHDSGKVLVELLNDILDLSKFDA
HHHHHHHHHCHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
GQLELEHIPFDLGSLIEDTANLLSQNAAPSVELTCLIDPHFPALVLGDPTRVRQIVSNLL
CCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEECCHHHHHHHHHHHH
SNALKFTRFGRVDVRLSTWKDGVRIEVCDTGIGIAQEAQVKIFQPFTQAGAGITRQYGGT
HHHHHHHHCCCEEEEEECCCCCCEEEEECCCCCCCCCCCEEEECCHHHHCCCCCHHCCCC
GLGLALTYNLCEAMQGRLTISSEVGFGSQFCAELPLPCHTRAVIPPPLHGKVLAITNAVS
CCHHHHHHHHHHHHCCCEEEECCCCCCHHHHHCCCCCCCCCCCCCCCCCCEEEEHHHHHH
GLAELLESLLPVWGLTYEQRTLDDPLLGLSPDVIITDCPECLFGLRPAVTAPILLVTAYG
HHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCEEEECCHHHHHCCCHHHHHHHHHEEHHC
SFMPGEEATALAPLQQQARPLARNALYQNLRRTLQPDLTTIDRARLESVPSQRRGRVLLV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHCCCEEEE
EDNPVNQLVAKGMLGKLGCDVVVAAHGVEALDQLEHDEFDLVLMDCNMPVMDGYEASRQI
CCCCHHHHHHHHHHHHCCCCEEEEHHCHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHH
RQSGRWPHLPIVALTANAMSEERERCRAAGMSDYLAKPFRREELAALLDQWIPTTTVL
HHCCCCCCCCEEEEEHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MVQHPMDIKFTHRLSYKQARLTVLVGFILGTLLSLLQIGIDYASEDASINREILSLLEIS
CCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHC
HNPASRIAYNIDAELAQELTLGLLRSPAIISARLTDNNQTVLASVKRPELQSGYRMISDF
CCCHHHEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCCCHHHHHHHHHHH
LFGAKRQFEDRLYLDHLPNESLGTLSLEVDTFAFGSRFLRRAEVTLLNGFARSLILTGIL
HHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LALFYVMLTKPLVRVIRELSGRDPRSAEPTTLECPGGHANDEIGVLVKVANQQFENIATE
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCEEEEEEHHHHHHHHHHH
IQQRRNAENRLTDYLAQLEDIVSARTAELKAINARLSQSNQELEVARSTALDMAEARSAF
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LANMSHEIRTPLNGLLGMIALSLDGPLNAEQQQQLSIAHDSGKVLVELLNDILDLSKFDA
HHHHHHHHHCHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
GQLELEHIPFDLGSLIEDTANLLSQNAAPSVELTCLIDPHFPALVLGDPTRVRQIVSNLL
CCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEECCHHHHHHHHHHHH
SNALKFTRFGRVDVRLSTWKDGVRIEVCDTGIGIAQEAQVKIFQPFTQAGAGITRQYGGT
HHHHHHHHCCCEEEEEECCCCCCEEEEECCCCCCCCCCCEEEECCHHHHCCCCCHHCCCC
GLGLALTYNLCEAMQGRLTISSEVGFGSQFCAELPLPCHTRAVIPPPLHGKVLAITNAVS
CCHHHHHHHHHHHHCCCEEEECCCCCCHHHHHCCCCCCCCCCCCCCCCCCEEEEHHHHHH
GLAELLESLLPVWGLTYEQRTLDDPLLGLSPDVIITDCPECLFGLRPAVTAPILLVTAYG
HHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCEEEECCHHHHHCCCHHHHHHHHHEEHHC
SFMPGEEATALAPLQQQARPLARNALYQNLRRTLQPDLTTIDRARLESVPSQRRGRVLLV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHCCCEEEE
EDNPVNQLVAKGMLGKLGCDVVVAAHGVEALDQLEHDEFDLVLMDCNMPVMDGYEASRQI
CCCCHHHHHHHHHHHHCCCCEEEEHHCHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHH
RQSGRWPHLPIVALTANAMSEERERCRAAGMSDYLAKPFRREELAALLDQWIPTTTVL
HHCCCCCCCCEEEEEHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]