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The map label for this gene is ynfL [H]

Identifier: 77460123

GI number: 77460123

Start: 4413435

End: 4414394

Strand: Direct

Name: ynfL [H]

Synonym: Pfl01_3902

Alternate gene names: 77460123

Gene position: 4413435-4414394 (Clockwise)

Preceding gene: 77460119

Following gene: 77460125

Centisome position: 68.55

GC content: 57.19

Gene sequence:

>960_bases
ATGACTTCTACTGCTAATCCTTGGGTCGGTCGGCGTTTTCTCAATGACCGGCTGGACTGGAACCTGCTGCGCACGTATCT
GGTGATCGGGCAGGAAGGCAGCATGAGCCGCGCTGCTGCGCGGTTGCACATCACCCAATCGGCGGTCAGTCAGGCGTTGA
AACGTCTCGAAGAGCAACTGGATTGTGTACTGATTGCGCGCAGCGGACGGCGCTTTGATCTGACGGAAACCGGGGAGGAA
GTCCTGCGGATTGCGGCGGACATCTACGGGGATATTTCCCGTCTGGGCACCGTGGTCGAGAATCGCCATGACGACGTGGT
GGGCAAGATCCGTATCCTCACCGTCAGTGGCGTTCAGGCGCGGCATTACGATGAGTTTCTTGCGGACTTCCACGAAACTC
ACCCGAAAATCGAGCTGGAAGTCGAGGTGATGGGCAGCTCGAACATCATCAGTTCATTGCTGCAGAAGACCGCTACGGTG
GGGGTCGGACTGTGTCGATTACCCCAACCAAGGTTGGAGCAACGGGTACTGTTTCGTGAGCGCTATGCGTACTTTTGCGG
ACAGCGACATCGATTGTTCGGGCAGCAGAATCTGACGCTGGAGCAACTGGCGGCGGAGAACTTCGTCAGCTTCACCAGCG
ATCAGCTCGGTGGCAATCTTTCGCCATTGACGCTGTTTCGTGACCAGCAGGGGTTTACCGGCAAGATCGTTGCTTCCTCC
ACCAGTTTCGAAGAGATTTATCGCCTGATCTGTGCGGGGTTCGGGATTGGTTGCTTGCCGATTCACCTGGTGCGACGGGA
TGTCGAACAGGGTTTGCTGTGGCGCTTGCCGCCGGAGGATGGGGTGGTGGATTTCGATATTCAGCTGCTGTGGAACCGCG
AGCAGAAGATGACTCAGGCGGAAACGGTATTCCTCGACAGCTTCCAGCACATGCTCAGCTTGCGTGAGCCTGTGCTGTGA

Upstream 100 bases:

>100_bases
GCTGACGGTCGCGCTCTGTGGCGCTTGTGGGCTTTATCGCTTCCGTGACAGGATTAGTAAATTTTCAAAAACTTATCTTT
ACTATTAGCGTGACTACTAC

Downstream 100 bases:

>100_bases
AGCTTTAGCGGGTTACACGTGAGGATCGCCCGGCGCTTTGGTCGGCGCCGCGTATTGCGGCTTGAGGTGACCGTCCTGGT
CGAGTAGCCAGGCGTCCATG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MTSTANPWVGRRFLNDRLDWNLLRTYLVIGQEGSMSRAAARLHITQSAVSQALKRLEEQLDCVLIARSGRRFDLTETGEE
VLRIAADIYGDISRLGTVVENRHDDVVGKIRILTVSGVQARHYDEFLADFHETHPKIELEVEVMGSSNIISSLLQKTATV
GVGLCRLPQPRLEQRVLFRERYAYFCGQRHRLFGQQNLTLEQLAAENFVSFTSDQLGGNLSPLTLFRDQQGFTGKIVASS
TSFEEIYRLICAGFGIGCLPIHLVRRDVEQGLLWRLPPEDGVVDFDIQLLWNREQKMTQAETVFLDSFQHMLSLREPVL

Sequences:

>Translated_319_residues
MTSTANPWVGRRFLNDRLDWNLLRTYLVIGQEGSMSRAAARLHITQSAVSQALKRLEEQLDCVLIARSGRRFDLTETGEE
VLRIAADIYGDISRLGTVVENRHDDVVGKIRILTVSGVQARHYDEFLADFHETHPKIELEVEVMGSSNIISSLLQKTATV
GVGLCRLPQPRLEQRVLFRERYAYFCGQRHRLFGQQNLTLEQLAAENFVSFTSDQLGGNLSPLTLFRDQQGFTGKIVASS
TSFEEIYRLICAGFGIGCLPIHLVRRDVEQGLLWRLPPEDGVVDFDIQLLWNREQKMTQAETVFLDSFQHMLSLREPVL
>Mature_318_residues
TSTANPWVGRRFLNDRLDWNLLRTYLVIGQEGSMSRAAARLHITQSAVSQALKRLEEQLDCVLIARSGRRFDLTETGEEV
LRIAADIYGDISRLGTVVENRHDDVVGKIRILTVSGVQARHYDEFLADFHETHPKIELEVEVMGSSNIISSLLQKTATVG
VGLCRLPQPRLEQRVLFRERYAYFCGQRHRLFGQQNLTLEQLAAENFVSFTSDQLGGNLSPLTLFRDQQGFTGKIVASST
SFEEIYRLICAGFGIGCLPIHLVRRDVEQGLLWRLPPEDGVVDFDIQLLWNREQKMTQAETVFLDSFQHMLSLREPVL

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=286, Percent_Identity=27.6223776223776, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI157672245, Length=225, Percent_Identity=26.2222222222222, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1786401, Length=255, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788296, Length=233, Percent_Identity=27.8969957081545, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787589, Length=130, Percent_Identity=31.5384615384615, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1788748, Length=245, Percent_Identity=22.0408163265306, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI145693105, Length=247, Percent_Identity=21.0526315789474, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87081904, Length=198, Percent_Identity=26.2626262626263, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87082132, Length=274, Percent_Identity=25.5474452554745, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787530, Length=238, Percent_Identity=23.5294117647059, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36263; Mature: 36132

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTANPWVGRRFLNDRLDWNLLRTYLVIGQEGSMSRAAARLHITQSAVSQALKRLEEQL
CCCCCCCHHHHHHHHHCCCHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
DCVLIARSGRRFDLTETGEEVLRIAADIYGDISRLGTVVENRHDDVVGKIRILTVSGVQA
CEEEEECCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCH
RHYDEFLADFHETHPKIELEVEVMGSSNIISSLLQKTATVGVGLCRLPQPRLEQRVLFRE
HHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHH
RYAYFCGQRHRLFGQQNLTLEQLAAENFVSFTSDQLGGNLSPLTLFRDQQGFTGKIVASS
HHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEECC
TSFEEIYRLICAGFGIGCLPIHLVRRDVEQGLLWRLPPEDGVVDFDIQLLWNREQKMTQA
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEECCCCCCEEEEEEEEEECCHHHHHHH
ETVFLDSFQHMLSLREPVL
HHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TSTANPWVGRRFLNDRLDWNLLRTYLVIGQEGSMSRAAARLHITQSAVSQALKRLEEQL
CCCCCCHHHHHHHHHCCCHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
DCVLIARSGRRFDLTETGEEVLRIAADIYGDISRLGTVVENRHDDVVGKIRILTVSGVQA
CEEEEECCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCH
RHYDEFLADFHETHPKIELEVEVMGSSNIISSLLQKTATVGVGLCRLPQPRLEQRVLFRE
HHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHH
RYAYFCGQRHRLFGQQNLTLEQLAAENFVSFTSDQLGGNLSPLTLFRDQQGFTGKIVASS
HHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEECC
TSFEEIYRLICAGFGIGCLPIHLVRRDVEQGLLWRLPPEDGVVDFDIQLLWNREQKMTQA
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEECCCCCCEEEEEEEEEECCHHHHHHH
ETVFLDSFQHMLSLREPVL
HHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]