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The map label for this gene is shiA [H]

Identifier: 77460122

GI number: 77460122

Start: 4411989

End: 4413290

Strand: Reverse

Name: shiA [H]

Synonym: Pfl01_3901

Alternate gene names: 77460122

Gene position: 4413290-4411989 (Counterclockwise)

Preceding gene: 77460124

Following gene: 77460121

Centisome position: 68.55

GC content: 62.14

Gene sequence:

>1302_bases
ATGCACACCACCGCTCAGCCGCGTCGCGCCGCGGCCGCTGCCTTCATTGGCACGACCATCGAGTTTTACGATTTCTACAT
CTACGCCACCGCCGCCGCACTGGTGCTCGGGCAAGTGTTCTTTCCCAGCAGCGATCCGGTGACCAGCACACTCGCGGCGT
TCGGCAGTTTCGCCGTCGGCTTCATCGCCCGCCCGATGGCCGGCATGGTGTTCGGACATCTGGGTGATCGCCTCGGTCGC
AAGAAAATGCTGCTGGTGACCATGGCACTGATGGGCCTCGCCACCGCCGGTATTGGTCTATTGCCGAGCTACGCCAGCGT
CGGCATCTGGGCGCCGATCGGGTTGATCGTATTACGCCTGATCCAGGGCATCTCCGTCGGCGGCGAGTGGGGCGGGGCGG
TGTTGATGGCCAGCGAACACGCCCCGGCCAAACGCAAAACCTTTTACGCCTCGTTCGCCCAGCTCGGCAGCCCGGCGGGT
TTGCTGCTGGCGTTGATCGCGTTTCGACTGGTGACGTCGCTGTCCCCGGAAGACTTCCTCGCCTGGGGATGGCGTCTGCC
GTTTCTTGCCAGCGGCGTGCTGATGATGGTCGGTCTGATGATCCGTTCCGGGGTGCATGAATCGCCGGAATTCGCCAAGG
CCAAAGACAACAACGAAACCGCCAAATACCCGGTGAAAGACGTGATCCGTCAGTGCTGGCGGCAGATCCTGTTTGCTGCC
GCCGCCGTCACCATCGGCTCGGCCGGGTTCTTCTTCACCAACACCTTCATGATCACCTACGTCACCCAATATCAGGGCAT
CCCAAGGGCGACCATTCTCGACAGCCTGTTCCTGGTGACGATCATTCAATTGCTCTCGCAACCGTTGTCGGCACTGCTCG
CCGAGCGCATCGGCGAGGGCCGATTCCTGATGCTGGTGGCGTTGCTGTGCACGGTCACGCCGTACCCGATGTTCCTGCTG
GTCGGCACTCAGAACATTCTGTTGATGACCCTCGGCATCGCCGTCGCGGTGGTGATTCTCTCGGCCCTGTACGCGGTGAT
CGCCGGGTACATGACCCAGGCCTTCCCGGTGCATCTGCGCTATTCGGGCATCTCCATCGCTTACCAACTGAGCTGCGCAG
TAGCCGGCGGCACCACGCCGCTGATCGGTACGCTGCTGGCCAGCAAGTTCTCCGGGCAGTGGCTGCCATTGGCGGTGTTC
TTCACGTTGCTGTCGGCCCTGTCTCTGATCGGTGTCTGTGGCCTCGCACGTCTGCGGGCCAACCCGGTCACCCTTCATAC
CAACAAAGAGGTGTATTCGTGA

Upstream 100 bases:

>100_bases
TAATCCTGTCACGGAAGCGATAAAGCCCACAAGCGCCACAGAGCGCGACCGTCAGCCGCCTCGATACCTGCCCAAAACTA
TAAATACACAGGGTTCTTGC

Downstream 100 bases:

>100_bases
GTAAGCCCGCCCACATTGACCCGATCGAATGGCAAGCCCGTTGCGAGCTCGCGGCGCTGTACCGCCTGATCGCGCACTTT
CGCATGACCGACCTGATCGA

Product: general substrate transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MHTTAQPRRAAAAAFIGTTIEFYDFYIYATAAALVLGQVFFPSSDPVTSTLAAFGSFAVGFIARPMAGMVFGHLGDRLGR
KKMLLVTMALMGLATAGIGLLPSYASVGIWAPIGLIVLRLIQGISVGGEWGGAVLMASEHAPAKRKTFYASFAQLGSPAG
LLLALIAFRLVTSLSPEDFLAWGWRLPFLASGVLMMVGLMIRSGVHESPEFAKAKDNNETAKYPVKDVIRQCWRQILFAA
AAVTIGSAGFFFTNTFMITYVTQYQGIPRATILDSLFLVTIIQLLSQPLSALLAERIGEGRFLMLVALLCTVTPYPMFLL
VGTQNILLMTLGIAVAVVILSALYAVIAGYMTQAFPVHLRYSGISIAYQLSCAVAGGTTPLIGTLLASKFSGQWLPLAVF
FTLLSALSLIGVCGLARLRANPVTLHTNKEVYS

Sequences:

>Translated_433_residues
MHTTAQPRRAAAAAFIGTTIEFYDFYIYATAAALVLGQVFFPSSDPVTSTLAAFGSFAVGFIARPMAGMVFGHLGDRLGR
KKMLLVTMALMGLATAGIGLLPSYASVGIWAPIGLIVLRLIQGISVGGEWGGAVLMASEHAPAKRKTFYASFAQLGSPAG
LLLALIAFRLVTSLSPEDFLAWGWRLPFLASGVLMMVGLMIRSGVHESPEFAKAKDNNETAKYPVKDVIRQCWRQILFAA
AAVTIGSAGFFFTNTFMITYVTQYQGIPRATILDSLFLVTIIQLLSQPLSALLAERIGEGRFLMLVALLCTVTPYPMFLL
VGTQNILLMTLGIAVAVVILSALYAVIAGYMTQAFPVHLRYSGISIAYQLSCAVAGGTTPLIGTLLASKFSGQWLPLAVF
FTLLSALSLIGVCGLARLRANPVTLHTNKEVYS
>Mature_433_residues
MHTTAQPRRAAAAAFIGTTIEFYDFYIYATAAALVLGQVFFPSSDPVTSTLAAFGSFAVGFIARPMAGMVFGHLGDRLGR
KKMLLVTMALMGLATAGIGLLPSYASVGIWAPIGLIVLRLIQGISVGGEWGGAVLMASEHAPAKRKTFYASFAQLGSPAG
LLLALIAFRLVTSLSPEDFLAWGWRLPFLASGVLMMVGLMIRSGVHESPEFAKAKDNNETAKYPVKDVIRQCWRQILFAA
AAVTIGSAGFFFTNTFMITYVTQYQGIPRATILDSLFLVTIIQLLSQPLSALLAERIGEGRFLMLVALLCTVTPYPMFLL
VGTQNILLMTLGIAVAVVILSALYAVIAGYMTQAFPVHLRYSGISIAYQLSCAVAGGTTPLIGTLLASKFSGQWLPLAVF
FTLLSALSLIGVCGLARLRANPVTLHTNKEVYS

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=411, Percent_Identity=39.4160583941606, Blast_Score=288, Evalue=5e-79,
Organism=Escherichia coli, GI1790550, Length=391, Percent_Identity=34.2710997442455, Blast_Score=237, Evalue=9e-64,
Organism=Escherichia coli, GI1789941, Length=391, Percent_Identity=34.7826086956522, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1788942, Length=397, Percent_Identity=29.9748110831234, Blast_Score=157, Evalue=9e-40,
Organism=Escherichia coli, GI87082231, Length=233, Percent_Identity=24.4635193133047, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46313; Mature: 46313

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCEEEECCCCCCC
>Mature Secondary Structure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CCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]