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The map label for this gene is pmpM [H]

Identifier: 77460108

GI number: 77460108

Start: 4396513

End: 4397913

Strand: Direct

Name: pmpM [H]

Synonym: Pfl01_3887

Alternate gene names: 77460108

Gene position: 4396513-4397913 (Clockwise)

Preceding gene: 77460106

Following gene: 77460111

Centisome position: 68.29

GC content: 65.67

Gene sequence:

>1401_bases
GTGACTGACCGCCCTGCTGCCGTTTCCCTCAACCGCCCTGCCCGGGTTCGCCTGGAGCTGAAGAATCTGCTCGGCCTGGC
CCTGCCGATCATGATCGCGCAGCTGGCAACCACCGCCATGGGCTTCGTCGATGCGGTGATGGCCGGGCGCGTCGGGCCCA
AGGATCTGGCGGCCGTGGCGCTGGGCAACTCGATCTGGGTGCCGGTGTTCCTGCTGATGACCGGCACTTTGCTGGCGACC
ACGCCTAAAGTCGCCCAGCGCTTTGGCGCCGGCACCCACAGCGAGATCGGCCCGATCGTGCGTCAGGCCCTGTGGCTGGC
ACTGGTGGTGGGCCTGATGGCGACCACCATGCTGTTCAGCGCCGAACCGATCCTGCATTTGATGAAAGTCGATCCGGAGC
TGATCGGCCCATGCATGCAGTACCTGCACGGCATCGCCAGCGGTCTGCCGGCCGTGGCGTTCTACCATGTGCTGCGCTGC
ACCAGTGACGGCCTCGGTCGCACCCGCCCGGCGATGGTGCTGGGCCTGTGCGGCCTGGCGCTGAACATTCCGCTCAACTA
CGTGTTCATTTATGGCCACTTTGGCGTACCGGCCATGGGCGGCGTCGGTTGCGGCTGGGCCACGGCGATCGTGATGTGGG
TGATGGCACTGGGTCTGGCCGGTTATGAGCGCTGGGCACCGGCCTACAAGTCGAGCGAACTGTTCAGCCGTTTCGACTGG
CCGCAATGGGCGGTGATCAAGCGTTTGCTGGCTATCGGTCTGCCGATCGGCGTCGCGGTGTTTGCCGAGTCGAGCATCTT
CGCGGTGATCGCGCTGCTGATCGGCAGCCTCGGCGCCACCGTGGTGGCCGGGCACCAGATCGCGCTGAACGTCAGCTCGC
TGGTGTTCATGATTCCGTACTCGCTGGGCATGGCCGTGACCGTGCGGGTCGGTCAGGCCCTGGGCCGTGAAGAACCTCGC
GAAGCACGTTTCGCGGCGGGTGTCGGCATGGGCACTGCGCTGGCTTACGCCTGCCTGTCGGCGAGCATGATGCTGCTGAT
GCGCGAGCACATTGCGGCGATCTACACGTCGGATCCGATGGTGATTCACATCGCCGCGATGCTGATCGTGTATTCGGCGC
TGTTCCAGTTTTCCGACGCGATTCAGGTCACCGCCGCCGGCGCCCTGCGCGGTTATCAGGACACTCGCGTGACGATGATT
CTGACCCTGTTCGCCTATTGGGGCATCGGCCTGCCGGTGGGTTACGCCCTCGGCCTGACCGACTGGCTCGGCGAGCCACG
CGGCCCGAGCGGCTTGTGGGAAGGCCTGATCGTCGGCCTGAGCTGCGCGGCGCTGATGCTGTCGATCCGCCTGACGCGCA
GTGCACGCAAACGCATTCGCATCAGCCGTTCGATGGGCTGA

Upstream 100 bases:

>100_bases
TACAAATCCGCAACAGAGGATTTTTCCTGACCATAACGTCAGCGGCGTAGAATGCCCGGCCTTGCGCATCAACCCCTTGG
ACGCTTTGCCTGTGAATTCC

Downstream 100 bases:

>100_bases
CCGGCCATAAAAAATGACCTGCACATGCAGGTCTTTTTTTTGCCTTCCGATCAACCGGACTTCTTGCGAATCCAGTACAG
ATAGGTCCCGGCTTCTTCGT

Product: multi anti extrusion protein MatE

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: H(+)/drug antiporter; Multidrug-efflux transporter [H]

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MTDRPAAVSLNRPARVRLELKNLLGLALPIMIAQLATTAMGFVDAVMAGRVGPKDLAAVALGNSIWVPVFLLMTGTLLAT
TPKVAQRFGAGTHSEIGPIVRQALWLALVVGLMATTMLFSAEPILHLMKVDPELIGPCMQYLHGIASGLPAVAFYHVLRC
TSDGLGRTRPAMVLGLCGLALNIPLNYVFIYGHFGVPAMGGVGCGWATAIVMWVMALGLAGYERWAPAYKSSELFSRFDW
PQWAVIKRLLAIGLPIGVAVFAESSIFAVIALLIGSLGATVVAGHQIALNVSSLVFMIPYSLGMAVTVRVGQALGREEPR
EARFAAGVGMGTALAYACLSASMMLLMREHIAAIYTSDPMVIHIAAMLIVYSALFQFSDAIQVTAAGALRGYQDTRVTMI
LTLFAYWGIGLPVGYALGLTDWLGEPRGPSGLWEGLIVGLSCAALMLSIRLTRSARKRIRISRSMG

Sequences:

>Translated_466_residues
MTDRPAAVSLNRPARVRLELKNLLGLALPIMIAQLATTAMGFVDAVMAGRVGPKDLAAVALGNSIWVPVFLLMTGTLLAT
TPKVAQRFGAGTHSEIGPIVRQALWLALVVGLMATTMLFSAEPILHLMKVDPELIGPCMQYLHGIASGLPAVAFYHVLRC
TSDGLGRTRPAMVLGLCGLALNIPLNYVFIYGHFGVPAMGGVGCGWATAIVMWVMALGLAGYERWAPAYKSSELFSRFDW
PQWAVIKRLLAIGLPIGVAVFAESSIFAVIALLIGSLGATVVAGHQIALNVSSLVFMIPYSLGMAVTVRVGQALGREEPR
EARFAAGVGMGTALAYACLSASMMLLMREHIAAIYTSDPMVIHIAAMLIVYSALFQFSDAIQVTAAGALRGYQDTRVTMI
LTLFAYWGIGLPVGYALGLTDWLGEPRGPSGLWEGLIVGLSCAALMLSIRLTRSARKRIRISRSMG
>Mature_465_residues
TDRPAAVSLNRPARVRLELKNLLGLALPIMIAQLATTAMGFVDAVMAGRVGPKDLAAVALGNSIWVPVFLLMTGTLLATT
PKVAQRFGAGTHSEIGPIVRQALWLALVVGLMATTMLFSAEPILHLMKVDPELIGPCMQYLHGIASGLPAVAFYHVLRCT
SDGLGRTRPAMVLGLCGLALNIPLNYVFIYGHFGVPAMGGVGCGWATAIVMWVMALGLAGYERWAPAYKSSELFSRFDWP
QWAVIKRLLAIGLPIGVAVFAESSIFAVIALLIGSLGATVVAGHQIALNVSSLVFMIPYSLGMAVTVRVGQALGREEPRE
ARFAAGVGMGTALAYACLSASMMLLMREHIAAIYTSDPMVIHIAAMLIVYSALFQFSDAIQVTAAGALRGYQDTRVTMIL
TLFAYWGIGLPVGYALGLTDWLGEPRGPSGLWEGLIVGLSCAALMLSIRLTRSARKRIRISRSMG

Specific function: Multidrug efflux pump that functions as an H(+)/drug antiporter. Confers resistance to benzalkonium chloride, fluoroquinolones, ethidium bromide, acriflavine and tetraphenylphosphonium chloride [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family [H]

Homologues:

Organism=Homo sapiens, GI22907060, Length=434, Percent_Identity=25.8064516129032, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI153792564, Length=406, Percent_Identity=23.6453201970443, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI38261960, Length=442, Percent_Identity=22.1719457013575, Blast_Score=83, Evalue=5e-16,
Organism=Escherichia coli, GI48994890, Length=434, Percent_Identity=47.2350230414747, Blast_Score=374, Evalue=1e-105,
Organism=Escherichia coli, GI226510954, Length=461, Percent_Identity=27.3318872017354, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6320545, Length=440, Percent_Identity=23.6363636363636, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6321821, Length=384, Percent_Identity=23.4375, Blast_Score=85, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 49674; Mature: 49543

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDRPAAVSLNRPARVRLELKNLLGLALPIMIAQLATTAMGFVDAVMAGRVGPKDLAAVA
CCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
LGNSIWVPVFLLMTGTLLATTPKVAQRFGAGTHSEIGPIVRQALWLALVVGLMATTMLFS
HCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEPILHLMKVDPELIGPCMQYLHGIASGLPAVAFYHVLRCTSDGLGRTRPAMVLGLCGLA
HHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
LNIPLNYVFIYGHFGVPAMGGVGCGWATAIVMWVMALGLAGYERWAPAYKSSELFSRFDW
HCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHCCC
PQWAVIKRLLAIGLPIGVAVFAESSIFAVIALLIGSLGATVVAGHQIALNVSSLVFMIPY
CHHHHHHHHHHHCCCHHHHHHHCHHHHHHHHHHHHCCCHHHHCCCHHEEEHHHHHHHHHH
SLGMAVTVRVGQALGREEPREARFAAGVGMGTALAYACLSASMMLLMREHIAAIYTSDPM
HHCHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
VIHIAAMLIVYSALFQFSDAIQVTAAGALRGYQDTRVTMILTLFAYWGIGLPVGYALGLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHH
DWLGEPRGPSGLWEGLIVGLSCAALMLSIRLTRSARKRIRISRSMG
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TDRPAAVSLNRPARVRLELKNLLGLALPIMIAQLATTAMGFVDAVMAGRVGPKDLAAVA
CCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
LGNSIWVPVFLLMTGTLLATTPKVAQRFGAGTHSEIGPIVRQALWLALVVGLMATTMLFS
HCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEPILHLMKVDPELIGPCMQYLHGIASGLPAVAFYHVLRCTSDGLGRTRPAMVLGLCGLA
HHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
LNIPLNYVFIYGHFGVPAMGGVGCGWATAIVMWVMALGLAGYERWAPAYKSSELFSRFDW
HCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHCCC
PQWAVIKRLLAIGLPIGVAVFAESSIFAVIALLIGSLGATVVAGHQIALNVSSLVFMIPY
CHHHHHHHHHHHCCCHHHHHHHCHHHHHHHHHHHHCCCHHHHCCCHHEEEHHHHHHHHHH
SLGMAVTVRVGQALGREEPREARFAAGVGMGTALAYACLSASMMLLMREHIAAIYTSDPM
HHCHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
VIHIAAMLIVYSALFQFSDAIQVTAAGALRGYQDTRVTMILTLFAYWGIGLPVGYALGLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHH
DWLGEPRGPSGLWEGLIVGLSCAALMLSIRLTRSARKRIRISRSMG
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]