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The map label for this gene is slt [H]

Identifier: 77460104

GI number: 77460104

Start: 4390847

End: 4392799

Strand: Direct

Name: slt [H]

Synonym: Pfl01_3883

Alternate gene names: 77460104

Gene position: 4390847-4392799 (Clockwise)

Preceding gene: 77460102

Following gene: 77460106

Centisome position: 68.2

GC content: 63.49

Gene sequence:

>1953_bases
ATGATTTCTCTGCCCGGATGTCTCATGCGCAGTCGCCTCTTCAGTGTTTTGTCATGTTTGCTGCTTTCCGCCGCTGCCGT
TCAATCCGCTCAGGCGGTGGACCTGTCCACCCAACGCCAGTATTACGATGAAGCCAAGCGCGCCCTGGCCAAGGGCGACA
GCGGCCCGTATTTCCGTTACAGCCAGGCTCTCGCCGATTATCCGCTGGAGCCGTACCTCGCCTACGACGAGCTGACTGCG
CGGCTGAAAACCGCCAGCAACGCGGAGATCGAGAAATTCCTCGCCGAGCACGGTGACCTGCCGCAGGCCAACTGGATGAA
ATTGCGCTGGCTGCGCTGGCTGGCCGATCGCGGCGATTGGGCGACGTTCGTCAAGTATTACGACCCGAAACTGAATTTCA
CTGAACTTGACTGCCTCCACGCGCAATACCAGATCAGCCACAACAAGAAGGCCGAGGGCTACGCCAACGCCGAGAAGCTC
TGGCTGACCGGCAAATCCCAGCCTGCCGCCTGTGATGCGCTGTTTGGCATGTGGGCCGCCGAAGGTCAGTTGACCGAACA
GAAACGCTGGGAGCGCGCCAAACTCGCCGCCCAACACCGCAACTATCCGCTGGCCAACAGCCTGGTCAACGGCCTGACCA
CCCTCGCCCCGCGCGGCCGCCTGCTGGTGGACGTGGCGCAGAAACCGGAACTGCTCAATCAGCCTTCGCGGTTCATGCCG
GCCGATGAACCGATGTCGGACGTGGTCAGCCTCGGCCTGCGCCGCCTCGCGCGTCAGGACCCGGACAAGGCCATGGCCCT
GCTCGACGGTTATGCCAGCAGCATGCATTTCTCCCGCGATGAAAAAGTCGCGATCGCCCGGGAAATCGGCCTGACCCTCG
CCCGGCGCTTCGACAGCCGCGCGCTGGACGTGATGACCAAGTACGACCCGGAACTGCGCGACAACACCGTGTCCGAATGG
CGCCTGCGGCTGCTGCTGCGTCTGGCGCGCTGGGACGACGCCTACCAACTGACCCGCCGTCTCCCGCAGGATCTGGCCAC
TACCAACCGCTGGCGCTACTGGCAGGCCCGCAGCCTGGAACTGGCGCAACCGCAAAACCCGGAAGCGCAGACGCTGTACA
AGAATCTGTCGCGGGAACGGGATTTCTACGGCTTCCTCGCCGCCGACCGCTCGCAATCGCCGTACTCGCTGAACAACAAG
CCGCTGGTGCTCAGTCAGGCGCTGATCAACAAGGTGCGCAACACCCCGGGCGTGCGTCGTGCGCTGGAGTTCCACGCCCG
TGGGCAGATCGTCGATGGCCGCCGCGAGTGGTATCACGTCAGCCGGCATTTCAGCCGCGACGAAATGGTCGCCCAGGCGA
AACTGGCCTACGACCTGAGATGGTACTTCCCGGCCATCCGCACCATCAGTCAGGCGCAGTACTGGGATGATCTGGACATC
CGTTTCCCGATGGCCCACCGCGACACTCTGGTGCGCGAAGCCAAGGTGCGCGGCCTGCATTCGAGCTGGGTGTTCGCCAT
CACCCGTCAGGAAAGTGCATTCATGGACGACGCCCGTTCCAGCGTCGGCGCCAGCGGCCTGATGCAACTGATGCCCGGCA
CCGCCAAGGAAACCGCGCGCAAGTTCAGCATTCCGCTGGCCTCGCCACAGCAGGTGCTGGATCCGGATAAAAACATCCAG
CTCGGCGCCGCTTACCTGAGCCAGGTGCACAGCCAGTTCAACGGCAACCGCGTGCTCGCCTCCGCCGCCTACAACGCCGG
CCCCGGCCGCGTGCGCCAGTGGCTGCGTGGCGCCGACCATCTGAGCTTCGACGTGTGGGTGGAAAGCATCCCGTTCGACG
AAACCCGCCAGTACGTGCAGAACGTGCTGTCCTACTCGGTGATCTACGGCCAGAAACTCAATTCGCCGCAACCGCTGGTG
GATTGGCATGAGCGGTATTTCGACGATCAGTGA

Upstream 100 bases:

>100_bases
GTTGCGAACTTGTGCTGCAAGGCCGGCAAAGCTTTCGCCCCTTGCTGGCAAAAGGCTAAGCTACAGGCAATTCAGTGCGG
GTTTTGAGCCGGCACTTGTC

Downstream 100 bases:

>100_bases
TCGGGGCGTGTTTCGCCGATAGATCGACGTAAACAAAAATGCCCGCATCGAGAGATGCGGGCATTTTTTATGGCGTTCTA
CTAGGTCGATTACTTAGTCG

Product: lytic transglycosylase catalytic subunit

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 650; Mature: 650

Protein sequence:

>650_residues
MISLPGCLMRSRLFSVLSCLLLSAAAVQSAQAVDLSTQRQYYDEAKRALAKGDSGPYFRYSQALADYPLEPYLAYDELTA
RLKTASNAEIEKFLAEHGDLPQANWMKLRWLRWLADRGDWATFVKYYDPKLNFTELDCLHAQYQISHNKKAEGYANAEKL
WLTGKSQPAACDALFGMWAAEGQLTEQKRWERAKLAAQHRNYPLANSLVNGLTTLAPRGRLLVDVAQKPELLNQPSRFMP
ADEPMSDVVSLGLRRLARQDPDKAMALLDGYASSMHFSRDEKVAIAREIGLTLARRFDSRALDVMTKYDPELRDNTVSEW
RLRLLLRLARWDDAYQLTRRLPQDLATTNRWRYWQARSLELAQPQNPEAQTLYKNLSRERDFYGFLAADRSQSPYSLNNK
PLVLSQALINKVRNTPGVRRALEFHARGQIVDGRREWYHVSRHFSRDEMVAQAKLAYDLRWYFPAIRTISQAQYWDDLDI
RFPMAHRDTLVREAKVRGLHSSWVFAITRQESAFMDDARSSVGASGLMQLMPGTAKETARKFSIPLASPQQVLDPDKNIQ
LGAAYLSQVHSQFNGNRVLASAAYNAGPGRVRQWLRGADHLSFDVWVESIPFDETRQYVQNVLSYSVIYGQKLNSPQPLV
DWHERYFDDQ

Sequences:

>Translated_650_residues
MISLPGCLMRSRLFSVLSCLLLSAAAVQSAQAVDLSTQRQYYDEAKRALAKGDSGPYFRYSQALADYPLEPYLAYDELTA
RLKTASNAEIEKFLAEHGDLPQANWMKLRWLRWLADRGDWATFVKYYDPKLNFTELDCLHAQYQISHNKKAEGYANAEKL
WLTGKSQPAACDALFGMWAAEGQLTEQKRWERAKLAAQHRNYPLANSLVNGLTTLAPRGRLLVDVAQKPELLNQPSRFMP
ADEPMSDVVSLGLRRLARQDPDKAMALLDGYASSMHFSRDEKVAIAREIGLTLARRFDSRALDVMTKYDPELRDNTVSEW
RLRLLLRLARWDDAYQLTRRLPQDLATTNRWRYWQARSLELAQPQNPEAQTLYKNLSRERDFYGFLAADRSQSPYSLNNK
PLVLSQALINKVRNTPGVRRALEFHARGQIVDGRREWYHVSRHFSRDEMVAQAKLAYDLRWYFPAIRTISQAQYWDDLDI
RFPMAHRDTLVREAKVRGLHSSWVFAITRQESAFMDDARSSVGASGLMQLMPGTAKETARKFSIPLASPQQVLDPDKNIQ
LGAAYLSQVHSQFNGNRVLASAAYNAGPGRVRQWLRGADHLSFDVWVESIPFDETRQYVQNVLSYSVIYGQKLNSPQPLV
DWHERYFDDQ
>Mature_650_residues
MISLPGCLMRSRLFSVLSCLLLSAAAVQSAQAVDLSTQRQYYDEAKRALAKGDSGPYFRYSQALADYPLEPYLAYDELTA
RLKTASNAEIEKFLAEHGDLPQANWMKLRWLRWLADRGDWATFVKYYDPKLNFTELDCLHAQYQISHNKKAEGYANAEKL
WLTGKSQPAACDALFGMWAAEGQLTEQKRWERAKLAAQHRNYPLANSLVNGLTTLAPRGRLLVDVAQKPELLNQPSRFMP
ADEPMSDVVSLGLRRLARQDPDKAMALLDGYASSMHFSRDEKVAIAREIGLTLARRFDSRALDVMTKYDPELRDNTVSEW
RLRLLLRLARWDDAYQLTRRLPQDLATTNRWRYWQARSLELAQPQNPEAQTLYKNLSRERDFYGFLAADRSQSPYSLNNK
PLVLSQALINKVRNTPGVRRALEFHARGQIVDGRREWYHVSRHFSRDEMVAQAKLAYDLRWYFPAIRTISQAQYWDDLDI
RFPMAHRDTLVREAKVRGLHSSWVFAITRQESAFMDDARSSVGASGLMQLMPGTAKETARKFSIPLASPQQVLDPDKNIQ
LGAAYLSQVHSQFNGNRVLASAAYNAGPGRVRQWLRGADHLSFDVWVESIPFDETRQYVQNVLSYSVIYGQKLNSPQPLV
DWHERYFDDQ

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=650, Percent_Identity=32.4615384615385, Blast_Score=299, Evalue=4e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 74615; Mature: 74615

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISLPGCLMRSRLFSVLSCLLLSAAAVQSAQAVDLSTQRQYYDEAKRALAKGDSGPYFRY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCHHH
SQALADYPLEPYLAYDELTARLKTASNAEIEKFLAEHGDLPQANWMKLRWLRWLADRGDW
HHHHHCCCCCCCEEHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCH
ATFVKYYDPKLNFTELDCLHAQYQISHNKKAEGYANAEKLWLTGKSQPAACDALFGMWAA
HHHHHHCCCCCCCHHHHHHHHHHEECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHC
EGQLTEQKRWERAKLAAQHRNYPLANSLVNGLTTLAPRGRLLVDVAQKPELLNQPSRFMP
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHCCCCCCCC
ADEPMSDVVSLGLRRLARQDPDKAMALLDGYASSMHFSRDEKVAIAREIGLTLARRFDSR
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
ALDVMTKYDPELRDNTVSEWRLRLLLRLARWDDAYQLTRRLPQDLATTNRWRYWQARSLE
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEECCCCC
LAQPQNPEAQTLYKNLSRERDFYGFLAADRSQSPYSLNNKPLVLSQALINKVRNTPGVRR
CCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHH
ALEFHARGQIVDGRREWYHVSRHFSRDEMVAQAKLAYDLRWYFPAIRTISQAQYWDDLDI
HHHHHHCCCEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
RFPMAHRDTLVREAKVRGLHSSWVFAITRQESAFMDDARSSVGASGLMQLMPGTAKETAR
ECCCHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCHHHHHHHCCCCHHHHHH
KFSIPLASPQQVLDPDKNIQLGAAYLSQVHSQFNGNRVLASAAYNAGPGRVRQWLRGADH
HHCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCC
LSFDVWVESIPFDETRQYVQNVLSYSVIYGQKLNSPQPLVDWHERYFDDQ
CEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MISLPGCLMRSRLFSVLSCLLLSAAAVQSAQAVDLSTQRQYYDEAKRALAKGDSGPYFRY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCHHH
SQALADYPLEPYLAYDELTARLKTASNAEIEKFLAEHGDLPQANWMKLRWLRWLADRGDW
HHHHHCCCCCCCEEHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCH
ATFVKYYDPKLNFTELDCLHAQYQISHNKKAEGYANAEKLWLTGKSQPAACDALFGMWAA
HHHHHHCCCCCCCHHHHHHHHHHEECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHC
EGQLTEQKRWERAKLAAQHRNYPLANSLVNGLTTLAPRGRLLVDVAQKPELLNQPSRFMP
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHCCCCCCCC
ADEPMSDVVSLGLRRLARQDPDKAMALLDGYASSMHFSRDEKVAIAREIGLTLARRFDSR
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
ALDVMTKYDPELRDNTVSEWRLRLLLRLARWDDAYQLTRRLPQDLATTNRWRYWQARSLE
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEECCCCC
LAQPQNPEAQTLYKNLSRERDFYGFLAADRSQSPYSLNNKPLVLSQALINKVRNTPGVRR
CCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHH
ALEFHARGQIVDGRREWYHVSRHFSRDEMVAQAKLAYDLRWYFPAIRTISQAQYWDDLDI
HHHHHHCCCEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
RFPMAHRDTLVREAKVRGLHSSWVFAITRQESAFMDDARSSVGASGLMQLMPGTAKETAR
ECCCHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCHHHHHHHCCCCHHHHHH
KFSIPLASPQQVLDPDKNIQLGAAYLSQVHSQFNGNRVLASAAYNAGPGRVRQWLRGADH
HHCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCC
LSFDVWVESIPFDETRQYVQNVLSYSVIYGQKLNSPQPLVDWHERYFDDQ
CEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]