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The map label for this gene is topA [H]

Identifier: 77460097

GI number: 77460097

Start: 4380704

End: 4383334

Strand: Reverse

Name: topA [H]

Synonym: Pfl01_3876

Alternate gene names: 77460097

Gene position: 4383334-4380704 (Counterclockwise)

Preceding gene: 77460098

Following gene: 77460096

Centisome position: 68.08

GC content: 60.55

Gene sequence:

>2631_bases
ATGGGCAAATCGCTGGTCATTGTGGAATCCCCGGCTAAGGCCAAGACCATCAACAAGTATCTGGGTAACCAATACGTGGT
GAAGTCGAGTATCGGCCATATCCGAGACCTGCCCACCAGCGGTTCGGCTAGCGCCAGCAAGGAGCCTGCCGCCAAGCGCG
GCAAGGCTGCTGCGGGCGAAGCGCCAGCGCTGTCGCCGAAAGAGAAGGCGCGCAAGCAGCTGGTCTCGCGCATGGGTGTC
GATCCCGAGCACGGCTGGAAAGCCAAGTACGAGATCCTCCCAGGCAAGGAAAAGGTCATCGAAGAGCTGCGCCGGCTCGC
CAAGGATGCTGACACCATCTATCTCGCAACCGACTTGGATCGCGAGGGGGAAGCCATTGCCTGGCACCTGCGCGAAGCCA
TCGGTGGTGACGACAGCCGCTACAAGCGCGTGGTGTTCAACGAAATCACCAAGAAGGCGATTCAGGAAGCCTTCTCGGAG
CCGGGCGAACTCGACATCGATCGAGTGAACGCGCAGCAGGCCCGTCGTTTCCTCGACCGCGTTGTCGGCTACATGGTTTC
GCCGCTGCTGTGGGCGAAGATCGCCCGTGGCCTGTCCGCCGGTCGCGTGCAATCGGTTGCCGTGAAGCTGGTGGTGGAGC
GTGAGCGCGAGATCCGTGCGTTCAACCCGGAAGAGTACTGGGAAGTGCACGCCGACCTCGGCACCGCCAAAGGCTCGACC
GTGCGTTTCGAAGTCGCTCGCGAGAAAGGCGAAGCGTTCAAGCCGCTGAACGAAGCGCAGGCCACGGCCGCGCTGGAGAA
GCTCAAGTCCTCCAGCTACAGCATCGTCAAGCGTGAAGACAAACCGACCAGCAGCAAGCCGTCGGCACCGTTCATCACCT
CCACACTGCAACAGGCCGCGAGCAACCGCCTGGGCTTCGGTGTGAAGAAAACCATGATGATGGCCCAGCGTCTGTACGAA
GCCGGCTACATCACCTATATGCGTACCGACTCGACCAACCTCTCGGCTGATGCCGTAGCGATGGCGCGCGATTACATCGA
AACCGAGTTCGGCAAGAAGTACCTGCCGGAGTCGCCGAACGTTTACAGCAGCAAGGAAGGCGCACAAGAGGCTCACGAAG
CGATTCGTCCTTCCGACGCCAACACCACGCCAGCCAAGCTGGCCGGCATGGAGCGCGACGCTGAGCGCCTCTACGAGCTG
ATCTGGCGCCAGTTCCTGGCTTGCCAGATGCTGCCGGCGCAATACCTGTCGACCACCGTCAGCGTCGCTGCTGGCGACTT
CGAGCTGCGCGCCAAGGGCCGCATCCTGAAGTTCGACGGTTACACCCGCGTGCTGCCGCAAATCACCAAGCCTGGCGACG
ATGACGTTCTGCCGGACATGGCGCAGGGCGACGTGATGAAGCTTATAAAGCTCGATCCGTCCCAGCACTTCACCAAGCCG
CCGGCCCGTTATTCGGAAGCCAGCCTGGTGAAGGAAATGGAAAAGCGCGGCATCGGTCGTCCTTCGACCTACGCAGCGAT
CATCTCGACCATTCAGGATCGCGGCTACGTGACCCTGCACAATCGTCGTTTCTATTCGGAAAAGATGGGCGACATCGTTA
CCGAGCGTCTGTCGGAAAGCTTCTCGAATCTCATGGACTACGGCTTCACCGCCGGCATGGAAGAGAACCTCGATGACGTG
GCCCAAGGCGAGCGCGACTGGAAAAACGTGCTGGACGAGTTCTACGGCGACTTCAAGAAAAAACTCGAAGTGGCTGAAAA
CCCAGAAGCTGGCATGCGTGCCAACCAGCCGGTGATGACCGACATTCCGTGCACCACTTGCGGCCGTCCAATGCAGATCC
GTACTGCATCGACCGGCGTGTTCCTCGGCTGCTCCGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCCACCGTCAAC
CTGGTGCCGGGCGATGAAATCGCGGCGGACGACGAGGGCGAGTCGGAATCCCTGGTACTGCGCGGCAAGCATCGCTGCCC
GATCTGCAGCACGGCGATGGACGCTTACCTGCTCGACGAGAAGCGCAAGCTGCACATCTGCGGTAACAACCCGGATTGCG
CCGGCTACGAGATCGAAGAGGGCAGCTACCGCATCAAGGGCTACGAAGGCCCGAGCCTGGAATGCGACAAGTGCGGCAGC
GAGATGCAGCTCAAGACCGGCCGTTTCGGCAAGTTCTTCGGTTGCACCAATCCGACGTGCAAGAACACTCGCAAACTGCT
GAAGAGCGGTGACGCGGCACCGCCGAAGATGGATCCGGTGAAGATGCCTGAGCTGAAATGCGAAAAGGTCAACGACACCT
ACATTCTGCGTGACGGTGCTTCCGGCCTGTTCCTGGCGGCTAGCCAGTTCCCGAAAAACCGCGAGACCCGTGCTCCGCTG
GTGATTGAGATTCTGCCGCACAAGGATGAGATCGATCCGAAGTATCACTTCCTGTGCGAAGCGCCGCAGAAGGATCCGGA
TGGTCTGCCAGCCGTGATCCGCTACAGCCGCAAGACCAAGGAGCAATACGTTCAGACCGAAGTCGACGGCAAGCCGACTG
GCTGGAAAGCATTCTTTGACGGTGGCAAATGGACGGTTGAAGACAAGCGTCCGGCGAAAGCCAAGGCTTGA

Upstream 100 bases:

>100_bases
AAACCCTGCGCTTGACCTCACCTTGTAGCCACTATATATAGCGGTGCCGCGTCAGGCGCCAACCGCCTTTCACTTCTAGA
ATTCAGGAATTTTCTGATCC

Downstream 100 bases:

>100_bases
GCAATCGCTGATCTGAAGGTGTCCTGACTGGGCATCTTCAATACAAAAGGCCGCATGACAACCTTCGGGTTTTCATGCGG
CCTTTTTGTTTAATGCTTGC

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 876; Mature: 875

Protein sequence:

>876_residues
MGKSLVIVESPAKAKTINKYLGNQYVVKSSIGHIRDLPTSGSASASKEPAAKRGKAAAGEAPALSPKEKARKQLVSRMGV
DPEHGWKAKYEILPGKEKVIEELRRLAKDADTIYLATDLDREGEAIAWHLREAIGGDDSRYKRVVFNEITKKAIQEAFSE
PGELDIDRVNAQQARRFLDRVVGYMVSPLLWAKIARGLSAGRVQSVAVKLVVEREREIRAFNPEEYWEVHADLGTAKGST
VRFEVAREKGEAFKPLNEAQATAALEKLKSSSYSIVKREDKPTSSKPSAPFITSTLQQAASNRLGFGVKKTMMMAQRLYE
AGYITYMRTDSTNLSADAVAMARDYIETEFGKKYLPESPNVYSSKEGAQEAHEAIRPSDANTTPAKLAGMERDAERLYEL
IWRQFLACQMLPAQYLSTTVSVAAGDFELRAKGRILKFDGYTRVLPQITKPGDDDVLPDMAQGDVMKLIKLDPSQHFTKP
PARYSEASLVKEMEKRGIGRPSTYAAIISTIQDRGYVTLHNRRFYSEKMGDIVTERLSESFSNLMDYGFTAGMEENLDDV
AQGERDWKNVLDEFYGDFKKKLEVAENPEAGMRANQPVMTDIPCTTCGRPMQIRTASTGVFLGCSGYSLPPKERCKATVN
LVPGDEIAADDEGESESLVLRGKHRCPICSTAMDAYLLDEKRKLHICGNNPDCAGYEIEEGSYRIKGYEGPSLECDKCGS
EMQLKTGRFGKFFGCTNPTCKNTRKLLKSGDAAPPKMDPVKMPELKCEKVNDTYILRDGASGLFLAASQFPKNRETRAPL
VIEILPHKDEIDPKYHFLCEAPQKDPDGLPAVIRYSRKTKEQYVQTEVDGKPTGWKAFFDGGKWTVEDKRPAKAKA

Sequences:

>Translated_876_residues
MGKSLVIVESPAKAKTINKYLGNQYVVKSSIGHIRDLPTSGSASASKEPAAKRGKAAAGEAPALSPKEKARKQLVSRMGV
DPEHGWKAKYEILPGKEKVIEELRRLAKDADTIYLATDLDREGEAIAWHLREAIGGDDSRYKRVVFNEITKKAIQEAFSE
PGELDIDRVNAQQARRFLDRVVGYMVSPLLWAKIARGLSAGRVQSVAVKLVVEREREIRAFNPEEYWEVHADLGTAKGST
VRFEVAREKGEAFKPLNEAQATAALEKLKSSSYSIVKREDKPTSSKPSAPFITSTLQQAASNRLGFGVKKTMMMAQRLYE
AGYITYMRTDSTNLSADAVAMARDYIETEFGKKYLPESPNVYSSKEGAQEAHEAIRPSDANTTPAKLAGMERDAERLYEL
IWRQFLACQMLPAQYLSTTVSVAAGDFELRAKGRILKFDGYTRVLPQITKPGDDDVLPDMAQGDVMKLIKLDPSQHFTKP
PARYSEASLVKEMEKRGIGRPSTYAAIISTIQDRGYVTLHNRRFYSEKMGDIVTERLSESFSNLMDYGFTAGMEENLDDV
AQGERDWKNVLDEFYGDFKKKLEVAENPEAGMRANQPVMTDIPCTTCGRPMQIRTASTGVFLGCSGYSLPPKERCKATVN
LVPGDEIAADDEGESESLVLRGKHRCPICSTAMDAYLLDEKRKLHICGNNPDCAGYEIEEGSYRIKGYEGPSLECDKCGS
EMQLKTGRFGKFFGCTNPTCKNTRKLLKSGDAAPPKMDPVKMPELKCEKVNDTYILRDGASGLFLAASQFPKNRETRAPL
VIEILPHKDEIDPKYHFLCEAPQKDPDGLPAVIRYSRKTKEQYVQTEVDGKPTGWKAFFDGGKWTVEDKRPAKAKA
>Mature_875_residues
GKSLVIVESPAKAKTINKYLGNQYVVKSSIGHIRDLPTSGSASASKEPAAKRGKAAAGEAPALSPKEKARKQLVSRMGVD
PEHGWKAKYEILPGKEKVIEELRRLAKDADTIYLATDLDREGEAIAWHLREAIGGDDSRYKRVVFNEITKKAIQEAFSEP
GELDIDRVNAQQARRFLDRVVGYMVSPLLWAKIARGLSAGRVQSVAVKLVVEREREIRAFNPEEYWEVHADLGTAKGSTV
RFEVAREKGEAFKPLNEAQATAALEKLKSSSYSIVKREDKPTSSKPSAPFITSTLQQAASNRLGFGVKKTMMMAQRLYEA
GYITYMRTDSTNLSADAVAMARDYIETEFGKKYLPESPNVYSSKEGAQEAHEAIRPSDANTTPAKLAGMERDAERLYELI
WRQFLACQMLPAQYLSTTVSVAAGDFELRAKGRILKFDGYTRVLPQITKPGDDDVLPDMAQGDVMKLIKLDPSQHFTKPP
ARYSEASLVKEMEKRGIGRPSTYAAIISTIQDRGYVTLHNRRFYSEKMGDIVTERLSESFSNLMDYGFTAGMEENLDDVA
QGERDWKNVLDEFYGDFKKKLEVAENPEAGMRANQPVMTDIPCTTCGRPMQIRTASTGVFLGCSGYSLPPKERCKATVNL
VPGDEIAADDEGESESLVLRGKHRCPICSTAMDAYLLDEKRKLHICGNNPDCAGYEIEEGSYRIKGYEGPSLECDKCGSE
MQLKTGRFGKFFGCTNPTCKNTRKLLKSGDAAPPKMDPVKMPELKCEKVNDTYILRDGASGLFLAASQFPKNRETRAPLV
IEILPHKDEIDPKYHFLCEAPQKDPDGLPAVIRYSRKTKEQYVQTEVDGKPTGWKAFFDGGKWTVEDKRPAKAKA

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=527, Percent_Identity=27.8937381404175, Blast_Score=165, Evalue=2e-40,
Organism=Homo sapiens, GI10835218, Length=608, Percent_Identity=26.4802631578947, Blast_Score=144, Evalue=4e-34,
Organism=Escherichia coli, GI1787529, Length=873, Percent_Identity=67.0103092783505, Blast_Score=1228, Evalue=0.0,
Organism=Escherichia coli, GI1788061, Length=551, Percent_Identity=23.5934664246824, Blast_Score=105, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI32563869, Length=532, Percent_Identity=27.4436090225564, Blast_Score=149, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI17555378, Length=516, Percent_Identity=25.3875968992248, Blast_Score=125, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6323263, Length=491, Percent_Identity=25.4582484725051, Blast_Score=120, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24585251, Length=517, Percent_Identity=27.4661508704062, Blast_Score=135, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24640096, Length=514, Percent_Identity=26.4591439688716, Blast_Score=132, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR013263
- InterPro:   IPR006171 [H]

Pfam domain/function: PF08272 Topo_Zn_Ribbon; PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 97444; Mature: 97313

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKSLVIVESPAKAKTINKYLGNQYVVKSSIGHIRDLPTSGSASASKEPAAKRGKAAAGE
CCCCEEEEECCCHHHHHHHHHCCCEEEHHHCCHHHCCCCCCCCCCCCCCHHHCCCCCCCC
APALSPKEKARKQLVSRMGVDPEHGWKAKYEILPGKEKVIEELRRLAKDADTIYLATDLD
CCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCC
REGEAIAWHLREAIGGDDSRYKRVVFNEITKKAIQEAFSEPGELDIDRVNAQQARRFLDR
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHH
VVGYMVSPLLWAKIARGLSAGRVQSVAVKLVVEREREIRAFNPEEYWEVHADLGTAKGST
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHEEHHHCCCCCCCE
VRFEVAREKGEAFKPLNEAQATAALEKLKSSSYSIVKREDKPTSSKPSAPFITSTLQQAA
EEEEEEHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHH
SNRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSADAVAMARDYIETEFGKKYLPESPN
HCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCHHHCCCCCC
VYSSKEGAQEAHEAIRPSDANTTPAKLAGMERDAERLYELIWRQFLACQMLPAQYLSTTV
CCCCCHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVAAGDFELRAKGRILKFDGYTRVLPQITKPGDDDVLPDMAQGDVMKLIKLDPSQHFTKP
HHCCCCEEEEECCCEEEECCCHHHHHCCCCCCCCCCCCCCCCCCCEEHEECCCHHHCCCC
PARYSEASLVKEMEKRGIGRPSTYAAIISTIQDRGYVTLHNRRFYSEKMGDIVTERLSES
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHH
FSNLMDYGFTAGMEENLDDVAQGERDWKNVLDEFYGDFKKKLEVAENPEAGMRANQPVMT
HHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEE
DIPCTTCGRPMQIRTASTGVFLGCSGYSLPPKERCKATVNLVPGDEIAADDEGESESLVL
CCCCCCCCCCEEEEEECCCEEEECCCCCCCHHHHCCEEEEECCCCCCCCCCCCCCCCEEE
RGKHRCPICSTAMDAYLLDEKRKLHICGNNPDCAGYEIEEGSYRIKGYEGPSLECDKCGS
ECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEECCCCEEEECCCCCCCCHHHCCC
EMQLKTGRFGKFFGCTNPTCKNTRKLLKSGDAAPPKMDPVKMPELKCEKVNDTYILRDGA
CCEECCCCCCCEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCC
SGLFLAASQFPKNRETRAPLVIEILPHKDEIDPKYHFLCEAPQKDPDGLPAVIRYSRKTK
CCEEEEHHHCCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHH
EQYVQTEVDGKPTGWKAFFDGGKWTVEDKRPAKAKA
HHHHHHCCCCCCCCCEEEECCCEEEECCCCCCCCCC
>Mature Secondary Structure 
GKSLVIVESPAKAKTINKYLGNQYVVKSSIGHIRDLPTSGSASASKEPAAKRGKAAAGE
CCCEEEEECCCHHHHHHHHHCCCEEEHHHCCHHHCCCCCCCCCCCCCCHHHCCCCCCCC
APALSPKEKARKQLVSRMGVDPEHGWKAKYEILPGKEKVIEELRRLAKDADTIYLATDLD
CCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCC
REGEAIAWHLREAIGGDDSRYKRVVFNEITKKAIQEAFSEPGELDIDRVNAQQARRFLDR
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHH
VVGYMVSPLLWAKIARGLSAGRVQSVAVKLVVEREREIRAFNPEEYWEVHADLGTAKGST
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHEEHHHCCCCCCCE
VRFEVAREKGEAFKPLNEAQATAALEKLKSSSYSIVKREDKPTSSKPSAPFITSTLQQAA
EEEEEEHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHH
SNRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSADAVAMARDYIETEFGKKYLPESPN
HCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCHHHCCCCCC
VYSSKEGAQEAHEAIRPSDANTTPAKLAGMERDAERLYELIWRQFLACQMLPAQYLSTTV
CCCCCHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVAAGDFELRAKGRILKFDGYTRVLPQITKPGDDDVLPDMAQGDVMKLIKLDPSQHFTKP
HHCCCCEEEEECCCEEEECCCHHHHHCCCCCCCCCCCCCCCCCCCEEHEECCCHHHCCCC
PARYSEASLVKEMEKRGIGRPSTYAAIISTIQDRGYVTLHNRRFYSEKMGDIVTERLSES
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHH
FSNLMDYGFTAGMEENLDDVAQGERDWKNVLDEFYGDFKKKLEVAENPEAGMRANQPVMT
HHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEE
DIPCTTCGRPMQIRTASTGVFLGCSGYSLPPKERCKATVNLVPGDEIAADDEGESESLVL
CCCCCCCCCCEEEEEECCCEEEECCCCCCCHHHHCCEEEEECCCCCCCCCCCCCCCCEEE
RGKHRCPICSTAMDAYLLDEKRKLHICGNNPDCAGYEIEEGSYRIKGYEGPSLECDKCGS
ECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEECCCCEEEECCCCCCCCHHHCCC
EMQLKTGRFGKFFGCTNPTCKNTRKLLKSGDAAPPKMDPVKMPELKCEKVNDTYILRDGA
CCEECCCCCCCEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCC
SGLFLAASQFPKNRETRAPLVIEILPHKDEIDPKYHFLCEAPQKDPDGLPAVIRYSRKTK
CCEEEEHHHCCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHH
EQYVQTEVDGKPTGWKAFFDGGKWTVEDKRPAKAKA
HHHHHHCCCCCCCCCEEEECCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]