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The map label for this gene is lexA1 [H]

Identifier: 77460093

GI number: 77460093

Start: 4378489

End: 4379097

Strand: Direct

Name: lexA1 [H]

Synonym: Pfl01_3872

Alternate gene names: 77460093

Gene position: 4378489-4379097 (Clockwise)

Preceding gene: 77460089

Following gene: 77460094

Centisome position: 68.01

GC content: 58.78

Gene sequence:

>609_bases
ATGCTTAAACTGACGCCACGCCAAGCCGAGATTCTGGCCTTTATCAAACGTTGCCTCGAAGACAACGGCTACCCGCCGAC
CCGTGCGGAAATCGCTCAGGAACTGGGTTTCAAGTCGCCCAATGCGGCCGAAGAGCACCTCAAGGCACTCGCCCGCAAAG
GCGCGATCGAGATGACTCCCGGCGCCTCGCGCGGCATTCGGATCCCCGGTTTTGAAGCCAAGGTCGACGAATCCACCCTG
CCGATCATCGGTCGAGTAGCTGCCGGCGCGCCGATTCTCGCCCAACAACACATCGAAGAGTCCTGCAACATCAACCCGGC
CTTCTTTCATCCGCGTGCCGACTACCTGTTGCGCGTTCATGGCATGAGCATGAAGGACATCGGCATTTTCGACGGTGATC
TGCTGGCGGTTCATACCACTCGCGAGGCACGCAACGGTCAGATCGTGGTTGCGCGGATCGGCGACGAAGTCACCGTCAAA
CGCTTCAAGCGTGACGGCAGCAAAGTCTGGCTGATTGCCGAAAACCCCGAGTTCGCCCCCATCGAAGTCAACCTGAAAGA
TCAGGAACTGGTGATCGAAGGCTTGAGTGTCGGCGTTATCCGCCGCTAA

Upstream 100 bases:

>100_bases
CGTCACTGACCGTCCGGCGGCATGGCAAAAAACGATCATTGCCAAGACCGCTTCACTGTATATAATCCCAGTCACTGTAT
AAAAAGACAGAGCGATCAAT

Downstream 100 bases:

>100_bases
AGGAGGCTTTATGCAGTTCCCACACGCACCTCAGCAAACACAATTGCCGTTGTTCGAAGCGTTTCTGGCGCAACCCCTCG
CCCCCATCCTCAAGGATGTC

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MLKLTPRQAEILAFIKRCLEDNGYPPTRAEIAQELGFKSPNAAEEHLKALARKGAIEMTPGASRGIRIPGFEAKVDESTL
PIIGRVAAGAPILAQQHIEESCNINPAFFHPRADYLLRVHGMSMKDIGIFDGDLLAVHTTREARNGQIVVARIGDEVTVK
RFKRDGSKVWLIAENPEFAPIEVNLKDQELVIEGLSVGVIRR

Sequences:

>Translated_202_residues
MLKLTPRQAEILAFIKRCLEDNGYPPTRAEIAQELGFKSPNAAEEHLKALARKGAIEMTPGASRGIRIPGFEAKVDESTL
PIIGRVAAGAPILAQQHIEESCNINPAFFHPRADYLLRVHGMSMKDIGIFDGDLLAVHTTREARNGQIVVARIGDEVTVK
RFKRDGSKVWLIAENPEFAPIEVNLKDQELVIEGLSVGVIRR
>Mature_202_residues
MLKLTPRQAEILAFIKRCLEDNGYPPTRAEIAQELGFKSPNAAEEHLKALARKGAIEMTPGASRGIRIPGFEAKVDESTL
PIIGRVAAGAPILAQQHIEESCNINPAFFHPRADYLLRVHGMSMKDIGIFDGDLLAVHTTREARNGQIVVARIGDEVTVK
RFKRDGSKVWLIAENPEFAPIEVNLKDQELVIEGLSVGVIRR

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family [H]

Homologues:

Organism=Escherichia coli, GI1790476, Length=201, Percent_Identity=63.681592039801, Blast_Score=256, Evalue=6e-70,
Organism=Escherichia coli, GI1787431, Length=125, Percent_Identity=34.4, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24 [H]

EC number: =3.4.21.88 [H]

Molecular weight: Translated: 22283; Mature: 22283

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKLTPRQAEILAFIKRCLEDNGYPPTRAEIAQELGFKSPNAAEEHLKALARKGAIEMTP
CCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEECC
GASRGIRIPGFEAKVDESTLPIIGRVAAGAPILAQQHIEESCNINPAFFHPRADYLLRVH
CCCCCEECCCCCCCCCCCCCCEEEHHHCCCCHHHHHHHHHHCCCCCEEECCCCCEEEEEC
GMSMKDIGIFDGDLLAVHTTREARNGQIVVARIGDEVTVKRFKRDGSKVWLIAENPEFAP
CCCCCCCCCCCCCEEEEEECCCCCCCCEEEEECCCHHHHHHHHCCCCEEEEEECCCCCCE
IEVNLKDQELVIEGLSVGVIRR
EEECCCHHHEEEECCCEEEECC
>Mature Secondary Structure
MLKLTPRQAEILAFIKRCLEDNGYPPTRAEIAQELGFKSPNAAEEHLKALARKGAIEMTP
CCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEECC
GASRGIRIPGFEAKVDESTLPIIGRVAAGAPILAQQHIEESCNINPAFFHPRADYLLRVH
CCCCCEECCCCCCCCCCCCCCEEEHHHCCCCHHHHHHHHHHCCCCCEEECCCCCEEEEEC
GMSMKDIGIFDGDLLAVHTTREARNGQIVVARIGDEVTVKRFKRDGSKVWLIAENPEFAP
CCCCCCCCCCCCCEEEEEECCCCCCCCEEEEECCCHHHHHHHHCCCCEEEEEECCCCCCE
IEVNLKDQELVIEGLSVGVIRR
EEECCCHHHEEEECCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]